Thiosulfatimonas sediminis
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2439 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6F8PWB1|A0A6F8PWB1_9GAMM Phenylalanine--tRNA ligase alpha subunit OS=Thiosulfatimonas sediminis OX=2675054 GN=pheS PE=3 SV=1
MM1 pKa = 7.64 GYY3 pKa = 10.67 DD4 pKa = 3.64 KK5 pKa = 11.29 DD6 pKa = 3.93 VDD8 pKa = 3.37 TGGYY12 pKa = 9.07 VEE14 pKa = 6.19 YY15 pKa = 10.6 IGAWDD20 pKa = 4.76 LNDD23 pKa = 5.08 DD24 pKa = 4.36 YY25 pKa = 11.44 IPYY28 pKa = 10.42 GSFGFTHH35 pKa = 7.44 KK36 pKa = 10.38 ISANTQWDD44 pKa = 3.32 IGSEE48 pKa = 4.25 VALDD52 pKa = 3.72 NPGQDD57 pKa = 3.34 FEE59 pKa = 5.27 IFFGLIQRR67 pKa = 11.84 DD68 pKa = 3.44
Molecular weight: 7.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.736
IPC2_protein 3.694
IPC_protein 3.643
Toseland 3.427
ProMoST 3.77
Dawson 3.668
Bjellqvist 3.897
Wikipedia 3.656
Rodwell 3.478
Grimsley 3.338
Solomon 3.63
Lehninger 3.592
Nozaki 3.808
DTASelect 4.062
Thurlkill 3.516
EMBOSS 3.656
Sillero 3.77
Patrickios 0.693
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.781
Protein with the highest isoelectric point:
>tr|A0A6F8PRC4|A0A6F8PRC4_9GAMM Thiol:disulfide interchange protein OS=Thiosulfatimonas sediminis OX=2675054 GN=dsbA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.49 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.92 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 NGRR28 pKa = 11.84 KK29 pKa = 9.16 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.89 GRR39 pKa = 11.84 KK40 pKa = 8.92 RR41 pKa = 11.84 LIPP44 pKa = 4.02
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.676
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.398
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2439
0
2439
786091
43
3553
322.3
35.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.182 ± 0.063
0.846 ± 0.015
5.405 ± 0.052
6.13 ± 0.05
4.089 ± 0.037
6.494 ± 0.054
2.239 ± 0.029
6.195 ± 0.046
5.274 ± 0.057
10.806 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.67 ± 0.026
4.283 ± 0.045
4.057 ± 0.035
5.847 ± 0.059
4.541 ± 0.043
6.122 ± 0.041
5.053 ± 0.063
6.623 ± 0.045
1.261 ± 0.023
2.883 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here