Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109)
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2011 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B1VGL6|B1VGL6_CORU7 Probable nicotinate-nucleotide adenylyltransferase OS=Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109) OX=504474 GN=nadD PE=3 SV=1
MM1 pKa = 7.02 VLSAACANFRR11 pKa = 11.84 GMTKK15 pKa = 10.06 PQDD18 pKa = 3.37 HH19 pKa = 7.0 HH20 pKa = 7.19 PDD22 pKa = 3.31 DD23 pKa = 4.3 TGAEE27 pKa = 4.3 GGDD30 pKa = 3.37 ASGPQPPQYY39 pKa = 9.84 RR40 pKa = 11.84 RR41 pKa = 11.84 PGDD44 pKa = 3.47 TPTADD49 pKa = 3.24 GSSQQGANPWAQPGAGPSAQQGAGQWGSQGSGQWGQAGAGYY90 pKa = 9.95 GAGASQQWAQQNPYY104 pKa = 9.15 GAQGQSAQWQGQDD117 pKa = 3.87 YY118 pKa = 9.71 GAQGYY123 pKa = 9.37 NNQGYY128 pKa = 10.14 GGPNYY133 pKa = 10.34 GNQGYY138 pKa = 8.98 PEE140 pKa = 3.98 QGYY143 pKa = 10.47 AGPGGQWQGQGYY155 pKa = 9.15 EE156 pKa = 3.95 NQGYY160 pKa = 8.42 QEE162 pKa = 4.05 QPEE165 pKa = 4.36 EE166 pKa = 4.22 KK167 pKa = 10.49 SNGRR171 pKa = 11.84 NILIIVLLVLALLAAVAFAVFWFTGKK197 pKa = 9.7 PGEE200 pKa = 4.61 GKK202 pKa = 10.34 SSEE205 pKa = 4.1 QTTVSSSEE213 pKa = 3.84 ATDD216 pKa = 3.3 ARR218 pKa = 11.84 GTEE221 pKa = 4.63 DD222 pKa = 4.22 EE223 pKa = 5.12 DD224 pKa = 5.98 ADD226 pKa = 3.94 AQGDD230 pKa = 4.7 DD231 pKa = 4.03 RR232 pKa = 11.84 DD233 pKa = 3.63 EE234 pKa = 4.45 DD235 pKa = 4.12 ANRR238 pKa = 11.84 EE239 pKa = 4.02 DD240 pKa = 5.32 DD241 pKa = 5.94 DD242 pKa = 5.3 EE243 pKa = 4.58 DD244 pKa = 3.84 TGDD247 pKa = 3.75 EE248 pKa = 4.35 AEE250 pKa = 4.75 SRR252 pKa = 11.84 PKK254 pKa = 10.63 NPRR257 pKa = 11.84 LPGYY261 pKa = 7.35 ATPVNSSARR270 pKa = 11.84 GSNPGGDD277 pKa = 4.21 LNSVWKK283 pKa = 10.55 SGPTSDD289 pKa = 4.78 SFALAVRR296 pKa = 11.84 DD297 pKa = 4.26 AYY299 pKa = 11.33 VDD301 pKa = 3.68 AYY303 pKa = 11.42 LDD305 pKa = 3.81 GDD307 pKa = 3.58 EE308 pKa = 6.08 DD309 pKa = 4.01 EE310 pKa = 5.07 FNHH313 pKa = 5.83 VVEE316 pKa = 5.43 AYY318 pKa = 10.8 SDD320 pKa = 3.79 VTGTSYY326 pKa = 11.02 TMSCRR331 pKa = 11.84 DD332 pKa = 3.09 NGEE335 pKa = 4.29 YY336 pKa = 9.8 VHH338 pKa = 6.46 CTGGNDD344 pKa = 3.39 ANVYY348 pKa = 9.43 IAA350 pKa = 5.1
Molecular weight: 36.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.961
IPC_protein 3.973
Toseland 3.757
ProMoST 4.113
Dawson 3.948
Bjellqvist 4.101
Wikipedia 3.872
Rodwell 3.795
Grimsley 3.668
Solomon 3.948
Lehninger 3.91
Nozaki 4.062
DTASelect 4.291
Thurlkill 3.795
EMBOSS 3.884
Sillero 4.088
Patrickios 1.049
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.969
Protein with the highest isoelectric point:
>tr|B1VEK0|B1VEK0_CORU7 Delta-aminolevulinic acid dehydratase OS=Corynebacterium urealyticum (strain ATCC 43042 / DSM 7109) OX=504474 GN=cu0229 PE=3 SV=1
MM1 pKa = 7.4 GSVIKK6 pKa = 10.38 KK7 pKa = 8.42 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.06 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.56 KK16 pKa = 9.27 HH17 pKa = 5.36 RR18 pKa = 11.84 KK19 pKa = 5.5 MLKK22 pKa = 7.41 RR23 pKa = 11.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 KK30 pKa = 10.11 LGKK33 pKa = 9.87
Molecular weight: 4.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.493
IPC2_protein 11.14
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.735
Grimsley 12.91
Solomon 13.349
Lehninger 13.261
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.364
Sillero 12.866
Patrickios 12.457
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.071
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2011
0
2011
702396
15
3618
349.3
37.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.974 ± 0.077
0.662 ± 0.015
5.854 ± 0.046
6.654 ± 0.056
3.123 ± 0.03
8.76 ± 0.05
2.1 ± 0.023
4.687 ± 0.037
3.461 ± 0.048
9.386 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.184 ± 0.025
2.871 ± 0.033
5.245 ± 0.045
3.618 ± 0.03
6.458 ± 0.05
5.743 ± 0.039
5.848 ± 0.038
7.873 ± 0.048
1.404 ± 0.022
2.094 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here