Achromobacter phage JWX
Average proteome isoelectric point is 6.49
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0B5A482|A0A0B5A482_9CAUD Terminase-like family protein OS=Achromobacter phage JWX OX=1589746 GN=JWX_00008 PE=4 SV=1
MM1 pKa = 8.18 DD2 pKa = 4.04 YY3 pKa = 10.99 SIDD6 pKa = 3.23 IHH8 pKa = 7.07 NLEE11 pKa = 4.43 EE12 pKa = 4.29 VISYY16 pKa = 10.35 ISNNLPCSDD25 pKa = 3.13 EE26 pKa = 4.0 MQRR29 pKa = 11.84 LVSQIEE35 pKa = 3.86 LRR37 pKa = 11.84 IAEE40 pKa = 4.41 LDD42 pKa = 3.72 EE43 pKa = 5.93 LNDD46 pKa = 3.74 LL47 pKa = 4.67
Molecular weight: 5.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.782
IPC2_protein 3.872
IPC_protein 3.732
Toseland 3.554
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.579
Grimsley 3.478
Solomon 3.681
Lehninger 3.63
Nozaki 3.859
DTASelect 3.999
Thurlkill 3.63
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.681
IPC2_peptide 3.821
IPC2.peptide.svr19 3.77
Protein with the highest isoelectric point:
>tr|A0A0B4ZZE8|A0A0B4ZZE8_9CAUD Uncharacterized protein OS=Achromobacter phage JWX OX=1589746 GN=JWX_00057 PE=4 SV=1
MM1 pKa = 7.85 AMVKK5 pKa = 10.3 CITPVGRR12 pKa = 11.84 VSFPSVSAASSYY24 pKa = 10.69 EE25 pKa = 3.68 GSAPKK30 pKa = 10.59 YY31 pKa = 8.68 SLKK34 pKa = 10.77 LAFEE38 pKa = 4.3 PEE40 pKa = 3.88 KK41 pKa = 11.05 VQNLKK46 pKa = 10.2 QALHH50 pKa = 5.94 YY51 pKa = 9.62 EE52 pKa = 4.1 QAEE55 pKa = 4.04 LLNRR59 pKa = 11.84 RR60 pKa = 11.84 KK61 pKa = 9.67 SAYY64 pKa = 10.14 HH65 pKa = 3.85 FTIRR69 pKa = 11.84 QAVRR73 pKa = 11.84 EE74 pKa = 4.05 MIRR77 pKa = 11.84 RR78 pKa = 11.84 RR79 pKa = 11.84 ITARR83 pKa = 11.84 LAEE86 pKa = 4.57 LLAASAKK93 pKa = 10.13 RR94 pKa = 11.84 AA95 pKa = 3.44
Molecular weight: 10.71 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.765
IPC_protein 10.379
Toseland 10.584
ProMoST 10.233
Dawson 10.701
Bjellqvist 10.394
Wikipedia 10.891
Rodwell 11.038
Grimsley 10.76
Solomon 10.774
Lehninger 10.745
Nozaki 10.555
DTASelect 10.379
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.628
Patrickios 10.789
IPC_peptide 10.774
IPC2_peptide 9.224
IPC2.peptide.svr19 8.616
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
61
0
61
14194
47
1160
232.7
25.84
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.511 ± 0.371
0.817 ± 0.121
5.721 ± 0.225
6.735 ± 0.292
3.607 ± 0.195
7.665 ± 0.322
1.761 ± 0.184
4.995 ± 0.174
5.39 ± 0.307
8.412 ± 0.24
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.783 ± 0.16
4.227 ± 0.238
4.946 ± 0.283
4.199 ± 0.283
5.777 ± 0.238
6.073 ± 0.279
5.432 ± 0.288
7.003 ± 0.233
1.86 ± 0.198
3.086 ± 0.161
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here