Rochambeau virus
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D3R216|A0A0D3R216_9RHAB Glycoprotein OS=Rochambeau virus OX=380435 PE=4 SV=1
MM1 pKa = 7.22 KK2 pKa = 10.33 VVFIIYY8 pKa = 8.89 TLLFSHH14 pKa = 6.94 LNSSEE19 pKa = 3.87 IFDD22 pKa = 5.11 DD23 pKa = 4.7 EE24 pKa = 5.02 EE25 pKa = 4.71 SSCDD29 pKa = 3.53 GNEE32 pKa = 4.09 LEE34 pKa = 5.79 KK35 pKa = 10.53 LQCMLDD41 pKa = 3.68 SLNSGGVLEE50 pKa = 5.24 SVPQTSTSPQGVGAIISGIEE70 pKa = 3.88 KK71 pKa = 10.33 PSFFLQLFLPFAMAFRR87 pKa = 11.84 DD88 pKa = 4.12 SIVV91 pKa = 2.78
Molecular weight: 9.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.251
IPC2_protein 4.19
IPC_protein 4.037
Toseland 3.872
ProMoST 4.177
Dawson 3.999
Bjellqvist 4.164
Wikipedia 3.91
Rodwell 3.872
Grimsley 3.783
Solomon 3.986
Lehninger 3.935
Nozaki 4.126
DTASelect 4.266
Thurlkill 3.91
EMBOSS 3.923
Sillero 4.151
Patrickios 3.465
IPC_peptide 3.986
IPC2_peptide 4.139
IPC2.peptide.svr19 4.056
Protein with the highest isoelectric point:
>tr|A0A0D3R1V8|A0A0D3R1V8_9RHAB Phosphoprotein OS=Rochambeau virus OX=380435 PE=4 SV=1
MM1 pKa = 7.48 EE2 pKa = 5.56 PVLEE6 pKa = 4.59 KK7 pKa = 10.94 NWGGNILEE15 pKa = 4.6 GFGKK19 pKa = 9.58 YY20 pKa = 7.09 WVEE23 pKa = 3.44 VRR25 pKa = 11.84 YY26 pKa = 8.72 WVSVSFWFFIGIILIKK42 pKa = 10.02 IIKK45 pKa = 9.51 SLVLIGEE52 pKa = 4.7 KK53 pKa = 9.89 IVSCSGAIYY62 pKa = 10.13 SIIKK66 pKa = 8.79 KK67 pKa = 9.11 HH68 pKa = 5.63 CLRR71 pKa = 11.84 IKK73 pKa = 9.1 RR74 pKa = 11.84 TTRR77 pKa = 11.84 RR78 pKa = 11.84 RR79 pKa = 11.84 RR80 pKa = 11.84 KK81 pKa = 9.14 IKK83 pKa = 9.86 KK84 pKa = 8.28 HH85 pKa = 5.0 HH86 pKa = 6.99 HH87 pKa = 6.24 NDD89 pKa = 3.23 PTRR92 pKa = 4.41
Molecular weight: 10.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.275
IPC2_protein 9.706
IPC_protein 9.926
Toseland 10.672
ProMoST 10.35
Dawson 10.76
Bjellqvist 10.379
Wikipedia 10.891
Rodwell 11.286
Grimsley 10.789
Solomon 10.818
Lehninger 10.789
Nozaki 10.643
DTASelect 10.379
Thurlkill 10.657
EMBOSS 11.052
Sillero 10.672
Patrickios 11.023
IPC_peptide 10.818
IPC2_peptide 9.136
IPC2.peptide.svr19 8.615
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
11
0
11
4388
74
2137
398.9
45.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.441 ± 0.415
1.755 ± 0.31
6.199 ± 0.524
8.159 ± 0.566
4.307 ± 0.507
6.085 ± 0.289
2.119 ± 0.223
6.996 ± 0.282
7.976 ± 0.548
10.255 ± 0.606
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.552 ± 0.29
4.444 ± 0.328
3.874 ± 0.385
2.302 ± 0.18
5.743 ± 0.618
8.706 ± 0.44
4.626 ± 0.317
5.082 ± 0.364
1.96 ± 0.12
3.418 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here