Elderberry carlavirus A
Average proteome isoelectric point is 6.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0A7MDS4|A0A0A7MDS4_9VIRU Triple gene block protein 1 OS=Elderberry carlavirus A OX=1569052 PE=4 SV=1
MM1 pKa = 7.98 DD2 pKa = 4.21 VLIKK6 pKa = 10.59 LALEE10 pKa = 4.1 RR11 pKa = 11.84 GFDD14 pKa = 4.26 RR15 pKa = 11.84 IHH17 pKa = 6.89 SNYY20 pKa = 9.77 SGCAVFHH27 pKa = 6.19 CVPGAGKK34 pKa = 6.92 TTLIRR39 pKa = 11.84 DD40 pKa = 4.13 LLQRR44 pKa = 11.84 DD45 pKa = 3.68 SRR47 pKa = 11.84 FQALTFGVVDD57 pKa = 3.86 KK58 pKa = 11.21 QSLSGRR64 pKa = 11.84 RR65 pKa = 11.84 IKK67 pKa = 10.9 GPSEE71 pKa = 3.57 RR72 pKa = 11.84 LEE74 pKa = 4.11 EE75 pKa = 4.39 GKK77 pKa = 8.59 LTIVDD82 pKa = 4.71 EE83 pKa = 4.33 YY84 pKa = 11.52 TEE86 pKa = 4.65 GDD88 pKa = 3.23 WEE90 pKa = 4.36 IYY92 pKa = 10.37 KK93 pKa = 10.35 PIALFGDD100 pKa = 5.22 PIQSCNLSRR109 pKa = 11.84 VKK111 pKa = 10.39 PSNFEE116 pKa = 3.67 CRR118 pKa = 11.84 RR119 pKa = 11.84 TFRR122 pKa = 11.84 FGRR125 pKa = 11.84 QTCALLGRR133 pKa = 11.84 LGFHH137 pKa = 6.41 ITSEE141 pKa = 4.12 FDD143 pKa = 3.49 DD144 pKa = 4.04 EE145 pKa = 5.32 VIIGDD150 pKa = 3.76 VFSIEE155 pKa = 4.06 PEE157 pKa = 3.92 GLIIAFEE164 pKa = 4.35 PEE166 pKa = 4.19 VKK168 pKa = 10.28 EE169 pKa = 3.76 LLEE172 pKa = 4.62 DD173 pKa = 3.76 HH174 pKa = 6.63 GCDD177 pKa = 3.58 YY178 pKa = 11.48 SEE180 pKa = 4.48 PCALRR185 pKa = 11.84 GRR187 pKa = 11.84 TTKK190 pKa = 10.73 AVTFITASEE199 pKa = 4.62 SIPEE203 pKa = 4.14 SLAHH207 pKa = 6.62 LFFIALTRR215 pKa = 11.84 HH216 pKa = 4.23 QEE218 pKa = 3.89 KK219 pKa = 10.84 LIVLSRR225 pKa = 11.84 NATFGATT232 pKa = 3.31
Molecular weight: 26.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.536
IPC2_protein 5.55
IPC_protein 5.525
Toseland 5.753
ProMoST 5.792
Dawson 5.69
Bjellqvist 5.741
Wikipedia 5.652
Rodwell 5.652
Grimsley 5.817
Solomon 5.69
Lehninger 5.664
Nozaki 5.906
DTASelect 6.071
Thurlkill 6.008
EMBOSS 5.97
Sillero 6.008
Patrickios 4.253
IPC_peptide 5.703
IPC2_peptide 6.008
IPC2.peptide.svr19 6.021
Protein with the highest isoelectric point:
>tr|A0A0A7M910|A0A0A7M910_9VIRU Capsid protein OS=Elderberry carlavirus A OX=1569052 PE=3 SV=1
MM1 pKa = 7.84 DD2 pKa = 4.79 RR3 pKa = 11.84 VKK5 pKa = 10.91 LMCDD9 pKa = 3.71 IIRR12 pKa = 11.84 ILSSEE17 pKa = 4.01 SRR19 pKa = 11.84 EE20 pKa = 3.92 AYY22 pKa = 10.41 YY23 pKa = 10.13 IDD25 pKa = 2.97 IARR28 pKa = 11.84 YY29 pKa = 8.59 IVHH32 pKa = 7.12 LSAEE36 pKa = 4.03 LHH38 pKa = 6.45 APGKK42 pKa = 9.11 STFAKK47 pKa = 9.85 RR48 pKa = 11.84 RR49 pKa = 11.84 RR50 pKa = 11.84 AKK52 pKa = 10.69 SLGRR56 pKa = 11.84 CHH58 pKa = 6.31 RR59 pKa = 11.84 CYY61 pKa = 10.46 RR62 pKa = 11.84 ISPGFYY68 pKa = 8.89 HH69 pKa = 5.52 TTKK72 pKa = 10.56 CDD74 pKa = 4.11 GISCTDD80 pKa = 3.2 SFGSRR85 pKa = 11.84 SKK87 pKa = 9.68 HH88 pKa = 5.23 RR89 pKa = 11.84 NYY91 pKa = 9.76 IVHH94 pKa = 6.51 GQRR97 pKa = 11.84 KK98 pKa = 7.88 HH99 pKa = 5.07 CC100 pKa = 4.36
Molecular weight: 11.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.231
IPC2_protein 9.311
IPC_protein 9.472
Toseland 9.809
ProMoST 9.648
Dawson 10.087
Bjellqvist 9.882
Wikipedia 10.292
Rodwell 10.321
Grimsley 10.175
Solomon 10.145
Lehninger 10.101
Nozaki 9.999
DTASelect 9.823
Thurlkill 9.94
EMBOSS 10.248
Sillero 10.058
Patrickios 7.512
IPC_peptide 10.131
IPC2_peptide 9.107
IPC2.peptide.svr19 8.081
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2807
72
1995
467.8
52.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.27 ± 0.587
3.206 ± 0.389
4.881 ± 0.345
6.947 ± 1.084
5.522 ± 0.854
6.056 ± 0.507
2.458 ± 0.706
5.949 ± 0.555
6.484 ± 0.599
10.046 ± 1.05
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.031 ± 0.336
4.239 ± 0.558
4.275 ± 0.628
2.814 ± 0.462
5.878 ± 0.985
7.731 ± 0.685
5.166 ± 0.555
5.771 ± 0.497
0.891 ± 0.184
3.384 ± 0.662
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here