Citricoccus muralis
Average proteome isoelectric point is 5.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3030 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3D9LDI5|A0A3D9LDI5_9MICC Serine transporter OS=Citricoccus muralis OX=169134 GN=C8E99_2325 PE=4 SV=1
MM1 pKa = 7.84 SDD3 pKa = 3.37 YY4 pKa = 10.85 EE5 pKa = 4.68 IYY7 pKa = 10.89 VEE9 pKa = 4.06 NCQGVIDD16 pKa = 4.15 AAYY19 pKa = 8.8 GTMADD24 pKa = 3.33 KK25 pKa = 10.25 TVNFSLMATEE35 pKa = 4.03 ATEE38 pKa = 3.84 AATALKK44 pKa = 10.25 PSFVVDD50 pKa = 3.53 SRR52 pKa = 11.84 GTHH55 pKa = 6.62 LDD57 pKa = 2.94 FGTYY61 pKa = 10.09 LDD63 pKa = 4.46 SFQAFYY69 pKa = 11.56 DD70 pKa = 3.73 NLLKK74 pKa = 10.78 PEE76 pKa = 4.35 FDD78 pKa = 4.5 DD79 pKa = 3.74 VCSDD83 pKa = 3.25 IEE85 pKa = 4.15 LVIGAFQTALNSYY98 pKa = 8.02 TAGDD102 pKa = 4.19 GEE104 pKa = 4.56 MLAQAKK110 pKa = 9.94 ARR112 pKa = 11.84 EE113 pKa = 4.38 DD114 pKa = 3.76 GVSEE118 pKa = 3.94 VGYY121 pKa = 7.94 PTVIAKK127 pKa = 10.38 DD128 pKa = 3.55 GSTLHH133 pKa = 6.65 EE134 pKa = 5.1 GGTAVRR140 pKa = 11.84 EE141 pKa = 4.17 DD142 pKa = 3.63 VNGDD146 pKa = 3.74 GVVDD150 pKa = 4.38 GQDD153 pKa = 2.7 TGTPQGEE160 pKa = 4.14 PGGLGVFEE168 pKa = 4.49 NQEE171 pKa = 4.1 QYY173 pKa = 11.01 QEE175 pKa = 3.95 ASEE178 pKa = 4.09
Molecular weight: 18.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.757
IPC_protein 3.745
Toseland 3.541
ProMoST 3.872
Dawson 3.732
Bjellqvist 3.923
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.452
Solomon 3.719
Lehninger 3.668
Nozaki 3.846
DTASelect 4.05
Thurlkill 3.592
EMBOSS 3.656
Sillero 3.859
Patrickios 1.036
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.797
Protein with the highest isoelectric point:
>tr|A0A3D9L987|A0A3D9L987_9MICC NitT/TauT family transport system substrate-binding protein OS=Citricoccus muralis OX=169134 GN=C8E99_0719 PE=4 SV=1
MM1 pKa = 7.6 KK2 pKa = 10.25 VRR4 pKa = 11.84 NSVRR8 pKa = 11.84 ALKK11 pKa = 9.9 KK12 pKa = 10.01 VPGAQVVRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 GRR24 pKa = 11.84 TFVINKK30 pKa = 9.03 KK31 pKa = 8.56 NPRR34 pKa = 11.84 MKK36 pKa = 10.47 ARR38 pKa = 11.84 QGG40 pKa = 3.24
Molecular weight: 4.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.111
IPC_protein 12.691
Toseland 12.852
ProMoST 13.349
Dawson 12.852
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.618
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.852
DTASelect 12.852
Thurlkill 12.852
EMBOSS 13.349
Sillero 12.852
Patrickios 12.34
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.103
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3030
0
3030
1053604
29
2148
347.7
37.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.763 ± 0.06
0.551 ± 0.01
5.989 ± 0.034
6.13 ± 0.043
2.918 ± 0.026
9.426 ± 0.035
2.211 ± 0.022
3.894 ± 0.033
2.011 ± 0.031
10.028 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.068 ± 0.018
2.111 ± 0.022
5.691 ± 0.033
3.29 ± 0.023
6.872 ± 0.043
5.803 ± 0.032
6.351 ± 0.035
8.488 ± 0.036
1.471 ± 0.021
1.935 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here