Erythrobacter sp. QSSC1-22B
Average proteome isoelectric point is 6.05
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3036 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1A7QIF4|A0A1A7QIF4_9SPHN Uncharacterized protein OS=Erythrobacter sp. QSSC1-22B OX=1860125 GN=A9995_08195 PE=4 SV=1
MM1 pKa = 7.46 LLAAPLAAQDD11 pKa = 3.58 AGAPGVRR18 pKa = 11.84 AEE20 pKa = 4.15 APEE23 pKa = 4.5 LPEE26 pKa = 3.95 STVFDD31 pKa = 3.75 GDD33 pKa = 4.09 YY34 pKa = 9.15 LTVGIGVGYY43 pKa = 9.04 GASYY47 pKa = 10.48 SGSDD51 pKa = 3.57 DD52 pKa = 3.09 YY53 pKa = 11.39 RR54 pKa = 11.84 TFVLPIVQGSLAGVDD69 pKa = 3.36 ISPRR73 pKa = 11.84 PAGVALDD80 pKa = 4.97 FIPDD84 pKa = 3.68 PDD86 pKa = 3.79 EE87 pKa = 4.4 GVSFSFGPVIKK98 pKa = 10.49 LNRR101 pKa = 11.84 DD102 pKa = 3.11 RR103 pKa = 11.84 ADD105 pKa = 3.49 LDD107 pKa = 4.46 GIKK110 pKa = 10.75 DD111 pKa = 3.94 PVVEE115 pKa = 5.14 AYY117 pKa = 10.55 GEE119 pKa = 4.4 LDD121 pKa = 3.28 TAIEE125 pKa = 4.35 VGPSAGVSFPQVLNAFDD142 pKa = 3.95 SLSFSVEE149 pKa = 4.02 AAWDD153 pKa = 3.42 VAGAHH158 pKa = 7.12 DD159 pKa = 4.57 GMSVSPAITYY169 pKa = 7.5 FTPLSRR175 pKa = 11.84 GIAASLSLSTKK186 pKa = 10.78 YY187 pKa = 10.29 IDD189 pKa = 5.56 DD190 pKa = 5.12 DD191 pKa = 4.03 YY192 pKa = 12.02 ADD194 pKa = 4.07 YY195 pKa = 10.75 YY196 pKa = 11.45 YY197 pKa = 11.02 SVPATNAALPAADD210 pKa = 4.23 VLPGFQADD218 pKa = 4.03 GGFEE222 pKa = 4.15 SAGVNVFMAFDD233 pKa = 4.39 LNGDD237 pKa = 3.71 VTDD240 pKa = 4.83 GGLALIALGGYY251 pKa = 9.73 SRR253 pKa = 11.84 MLGDD257 pKa = 4.63 AKK259 pKa = 10.77 DD260 pKa = 3.76 SPFTSIRR267 pKa = 11.84 GEE269 pKa = 3.67 ADD271 pKa = 2.88 QFLGALGIGYY281 pKa = 7.14 TFF283 pKa = 4.27
Molecular weight: 29.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.656
IPC_protein 3.694
Toseland 3.452
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.668
Rodwell 3.516
Grimsley 3.363
Solomon 3.694
Lehninger 3.643
Nozaki 3.808
DTASelect 4.101
Thurlkill 3.516
EMBOSS 3.668
Sillero 3.821
Patrickios 1.875
IPC_peptide 3.681
IPC2_peptide 3.783
IPC2.peptide.svr19 3.744
Protein with the highest isoelectric point:
>tr|A0A1A7QFK8|A0A1A7QFK8_9SPHN Uncharacterized protein OS=Erythrobacter sp. QSSC1-22B OX=1860125 GN=A9995_13480 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 QRR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.72 GFFARR21 pKa = 11.84 KK22 pKa = 7.44 ATPGGRR28 pKa = 11.84 NVLRR32 pKa = 11.84 NRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 6.15 TLCAA44 pKa = 4.05
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.438
IPC2_protein 10.935
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.31
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.047
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.117
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3036
0
3036
946070
41
1567
311.6
33.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.74 ± 0.065
0.819 ± 0.015
6.049 ± 0.032
6.306 ± 0.042
3.643 ± 0.029
8.841 ± 0.04
1.944 ± 0.022
5.025 ± 0.031
2.84 ± 0.04
10.046 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.479 ± 0.025
2.482 ± 0.025
5.137 ± 0.033
3.245 ± 0.024
7.288 ± 0.043
5.358 ± 0.031
5.21 ± 0.028
7.003 ± 0.034
1.402 ± 0.02
2.144 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here