Amino acid dipepetide frequency for Erythrobacter sp. QSSC1-22B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.019AlaAla: 17.019 ± 0.188
1.093AlaCys: 1.093 ± 0.039
7.026AlaAsp: 7.026 ± 0.095
8.195AlaGlu: 8.195 ± 0.119
4.103AlaPhe: 4.103 ± 0.074
10.966AlaGly: 10.966 ± 0.131
2.178AlaHis: 2.178 ± 0.048
6.763AlaIle: 6.763 ± 0.103
3.895AlaLys: 3.895 ± 0.08
14.086AlaLeu: 14.086 ± 0.173
3.913AlaMet: 3.913 ± 0.07
3.086AlaAsn: 3.086 ± 0.053
5.731AlaPro: 5.731 ± 0.099
4.905AlaGln: 4.905 ± 0.081
9.407AlaArg: 9.407 ± 0.12
6.637AlaSer: 6.637 ± 0.095
6.189AlaThr: 6.189 ± 0.088
8.249AlaVal: 8.249 ± 0.107
1.488AlaTrp: 1.488 ± 0.043
2.466AlaTyr: 2.466 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
1.08CysAla: 1.08 ± 0.039
0.103CysCys: 0.103 ± 0.01
0.594CysAsp: 0.594 ± 0.026
0.501CysGlu: 0.501 ± 0.024
0.266CysPhe: 0.266 ± 0.018
0.859CysGly: 0.859 ± 0.029
0.221CysHis: 0.221 ± 0.015
0.385CysIle: 0.385 ± 0.019
0.163CysLys: 0.163 ± 0.014
0.711CysLeu: 0.711 ± 0.027
0.134CysMet: 0.134 ± 0.011
0.23CysAsn: 0.23 ± 0.016
0.428CysPro: 0.428 ± 0.025
0.246CysGln: 0.246 ± 0.015
0.546CysArg: 0.546 ± 0.024
0.487CysSer: 0.487 ± 0.024
0.422CysThr: 0.422 ± 0.021
0.541CysVal: 0.541 ± 0.023
0.112CysTrp: 0.112 ± 0.01
0.159CysTyr: 0.159 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.056AspAla: 7.056 ± 0.098
0.591AspCys: 0.591 ± 0.025
3.464AspAsp: 3.464 ± 0.067
4.165AspGlu: 4.165 ± 0.067
2.249AspPhe: 2.249 ± 0.051
5.693AspGly: 5.693 ± 0.091
1.315AspHis: 1.315 ± 0.045
2.964AspIle: 2.964 ± 0.061
1.681AspLys: 1.681 ± 0.054
5.905AspLeu: 5.905 ± 0.076
1.421AspMet: 1.421 ± 0.038
1.458AspAsn: 1.458 ± 0.043
3.891AspPro: 3.891 ± 0.069
1.794AspGln: 1.794 ± 0.045
4.991AspArg: 4.991 ± 0.07
2.633AspSer: 2.633 ± 0.049
2.845AspThr: 2.845 ± 0.056
3.685AspVal: 3.685 ± 0.067
1.119AspTrp: 1.119 ± 0.04
1.569AspTyr: 1.569 ± 0.046
0.0AspXaa: 0.0 ± 0.0
Glu
8.256GluAla: 8.256 ± 0.113
0.39GluCys: 0.39 ± 0.019
3.435GluAsp: 3.435 ± 0.056
3.831GluGlu: 3.831 ± 0.084
2.006GluPhe: 2.006 ± 0.046
5.13GluGly: 5.13 ± 0.08
1.331GluHis: 1.331 ± 0.042
3.383GluIle: 3.383 ± 0.062
1.942GluLys: 1.942 ± 0.051
6.226GluLeu: 6.226 ± 0.08
1.681GluMet: 1.681 ± 0.045
1.629GluAsn: 1.629 ± 0.046
3.127GluPro: 3.127 ± 0.058
2.523GluGln: 2.523 ± 0.056
5.814GluArg: 5.814 ± 0.085
2.689GluSer: 2.689 ± 0.057
3.583GluThr: 3.583 ± 0.07
4.1GluVal: 4.1 ± 0.066
0.91GluTrp: 0.91 ± 0.031
1.077GluTyr: 1.077 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
5.126PheAla: 5.126 ± 0.077
0.322PheCys: 0.322 ± 0.018
2.8PheAsp: 2.8 ± 0.058
2.3PheGlu: 2.3 ± 0.047
1.333PhePhe: 1.333 ± 0.041
3.807PheGly: 3.807 ± 0.075
0.69PheHis: 0.69 ± 0.03
1.491PheIle: 1.491 ± 0.043
0.838PheLys: 0.838 ± 0.03
3.168PheLeu: 3.168 ± 0.075
0.76PheMet: 0.76 ± 0.029
0.982PheAsn: 0.982 ± 0.038
1.521PhePro: 1.521 ± 0.037
0.914PheGln: 0.914 ± 0.033
2.095PheArg: 2.095 ± 0.047
2.032PheSer: 2.032 ± 0.051
2.059PheThr: 2.059 ± 0.048
2.73PheVal: 2.73 ± 0.057
0.552PheTrp: 0.552 ± 0.027
0.904PheTyr: 0.904 ± 0.036
0.0PheXaa: 0.0 ± 0.0
Gly
9.695GlyAla: 9.695 ± 0.114
0.861GlyCys: 0.861 ± 0.029
5.107GlyAsp: 5.107 ± 0.068
5.925GlyGlu: 5.925 ± 0.093
3.786GlyPhe: 3.786 ± 0.059
7.91GlyGly: 7.91 ± 0.125
1.793GlyHis: 1.793 ± 0.046
4.476GlyIle: 4.476 ± 0.073
3.081GlyLys: 3.081 ± 0.067
8.732GlyLeu: 8.732 ± 0.107
2.403GlyMet: 2.403 ± 0.053
2.163GlyAsn: 2.163 ± 0.056
3.605GlyPro: 3.605 ± 0.064
2.982GlyGln: 2.982 ± 0.064
6.01GlyArg: 6.01 ± 0.099
5.203GlySer: 5.203 ± 0.087
4.856GlyThr: 4.856 ± 0.069
5.973GlyVal: 5.973 ± 0.077
1.56GlyTrp: 1.56 ± 0.046
2.287GlyTyr: 2.287 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
2.276HisAla: 2.276 ± 0.058
0.264HisCys: 0.264 ± 0.015
1.214HisAsp: 1.214 ± 0.036
1.115HisGlu: 1.115 ± 0.038
0.801HisPhe: 0.801 ± 0.031
1.939HisGly: 1.939 ± 0.055
0.506HisHis: 0.506 ± 0.021
0.914HisIle: 0.914 ± 0.031
0.465HisLys: 0.465 ± 0.022
1.849HisLeu: 1.849 ± 0.05
0.392HisMet: 0.392 ± 0.021
0.426HisAsn: 0.426 ± 0.023
1.262HisPro: 1.262 ± 0.041
0.497HisGln: 0.497 ± 0.022
1.543HisArg: 1.543 ± 0.043
1.068HisSer: 1.068 ± 0.038
0.774HisThr: 0.774 ± 0.029
1.288HisVal: 1.288 ± 0.037
0.336HisTrp: 0.336 ± 0.02
0.512HisTyr: 0.512 ± 0.026
0.0HisXaa: 0.0 ± 0.0
Ile
7.926IleAla: 7.926 ± 0.092
0.449IleCys: 0.449 ± 0.021
3.824IleAsp: 3.824 ± 0.057
3.945IleGlu: 3.945 ± 0.069
1.646IlePhe: 1.646 ± 0.044
5.171IleGly: 5.171 ± 0.079
0.898IleHis: 0.898 ± 0.031
2.058IleIle: 2.058 ± 0.057
1.195IleLys: 1.195 ± 0.036
3.807IleLeu: 3.807 ± 0.062
0.922IleMet: 0.922 ± 0.029
1.268IleAsn: 1.268 ± 0.045
2.312IlePro: 2.312 ± 0.047
1.106IleGln: 1.106 ± 0.034
3.146IleArg: 3.146 ± 0.063
2.528IleSer: 2.528 ± 0.053
2.43IleThr: 2.43 ± 0.05
3.968IleVal: 3.968 ± 0.069
0.62IleTrp: 0.62 ± 0.028
1.032IleTyr: 1.032 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
3.418LysAla: 3.418 ± 0.074
0.161LysCys: 0.161 ± 0.013
1.458LysAsp: 1.458 ± 0.047
1.332LysGlu: 1.332 ± 0.045
0.815LysPhe: 0.815 ± 0.032
2.341LysGly: 2.341 ± 0.06
0.598LysHis: 0.598 ± 0.024
1.388LysIle: 1.388 ± 0.046
1.062LysLys: 1.062 ± 0.044
3.168LysLeu: 3.168 ± 0.056
0.7LysMet: 0.7 ± 0.03
0.687LysAsn: 0.687 ± 0.027
1.821LysPro: 1.821 ± 0.053
0.946LysGln: 0.946 ± 0.037
2.35LysArg: 2.35 ± 0.054
1.608LysSer: 1.608 ± 0.042
1.642LysThr: 1.642 ± 0.048
1.965LysVal: 1.965 ± 0.047
0.342LysTrp: 0.342 ± 0.019
0.604LysTyr: 0.604 ± 0.024
0.0LysXaa: 0.0 ± 0.0
Leu
14.81LeuAla: 14.81 ± 0.177
0.759LeuCys: 0.759 ± 0.032
6.417LeuAsp: 6.417 ± 0.085
5.82LeuGlu: 5.82 ± 0.087
3.654LeuPhe: 3.654 ± 0.076
8.567LeuGly: 8.567 ± 0.121
1.749LeuHis: 1.749 ± 0.043
4.67LeuIle: 4.67 ± 0.075
2.71LeuLys: 2.71 ± 0.061
9.739LeuLeu: 9.739 ± 0.152
2.133LeuMet: 2.133 ± 0.047
2.235LeuAsn: 2.235 ± 0.047
5.447LeuPro: 5.447 ± 0.08
2.608LeuGln: 2.608 ± 0.054
7.003LeuArg: 7.003 ± 0.093
5.778LeuSer: 5.778 ± 0.087
5.556LeuThr: 5.556 ± 0.075
7.679LeuVal: 7.679 ± 0.111
1.203LeuTrp: 1.203 ± 0.036
1.927LeuTyr: 1.927 ± 0.046
0.0LeuXaa: 0.0 ± 0.0
Met
3.337MetAla: 3.337 ± 0.053
0.159MetCys: 0.159 ± 0.012
1.175MetAsp: 1.175 ± 0.045
1.243MetGlu: 1.243 ± 0.04
0.706MetPhe: 0.706 ± 0.031
1.968MetGly: 1.968 ± 0.051
0.453MetHis: 0.453 ± 0.022
1.402MetIle: 1.402 ± 0.043
0.884MetLys: 0.884 ± 0.03
2.633MetLeu: 2.633 ± 0.057
0.705MetMet: 0.705 ± 0.029
0.699MetAsn: 0.699 ± 0.029
1.454MetPro: 1.454 ± 0.035
0.82MetGln: 0.82 ± 0.026
1.88MetArg: 1.88 ± 0.044
1.43MetSer: 1.43 ± 0.043
1.668MetThr: 1.668 ± 0.05
1.689MetVal: 1.689 ± 0.04
0.236MetTrp: 0.236 ± 0.017
0.246MetTyr: 0.246 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.054AsnAla: 3.054 ± 0.06
0.274AsnCys: 0.274 ± 0.018
1.438AsnAsp: 1.438 ± 0.042
1.265AsnGlu: 1.265 ± 0.038
0.982AsnPhe: 0.982 ± 0.038
2.147AsnGly: 2.147 ± 0.057
0.481AsnHis: 0.481 ± 0.019
1.253AsnIle: 1.253 ± 0.038
0.586AsnLys: 0.586 ± 0.026
2.458AsnLeu: 2.458 ± 0.054
0.525AsnMet: 0.525 ± 0.023
0.597AsnAsn: 0.597 ± 0.029
1.741AsnPro: 1.741 ± 0.048
0.778AsnGln: 0.778 ± 0.027
1.924AsnArg: 1.924 ± 0.048
1.379AsnSer: 1.379 ± 0.042
1.182AsnThr: 1.182 ± 0.038
1.692AsnVal: 1.692 ± 0.044
0.409AsnTrp: 0.409 ± 0.02
0.662AsnTyr: 0.662 ± 0.031
0.0AsnXaa: 0.0 ± 0.0
Pro
6.279ProAla: 6.279 ± 0.094
0.333ProCys: 0.333 ± 0.019
3.714ProAsp: 3.714 ± 0.063
4.135ProGlu: 4.135 ± 0.065
1.884ProPhe: 1.884 ± 0.043
4.583ProGly: 4.583 ± 0.073
1.013ProHis: 1.013 ± 0.035
2.46ProIle: 2.46 ± 0.051
1.368ProLys: 1.368 ± 0.042
4.936ProLeu: 4.936 ± 0.066
1.185ProMet: 1.185 ± 0.036
1.152ProAsn: 1.152 ± 0.032
2.583ProPro: 2.583 ± 0.071
1.854ProGln: 1.854 ± 0.049
3.021ProArg: 3.021 ± 0.054
2.631ProSer: 2.631 ± 0.053
2.479ProThr: 2.479 ± 0.055
4.091ProVal: 4.091 ± 0.078
0.625ProTrp: 0.625 ± 0.027
1.044ProTyr: 1.044 ± 0.034
0.0ProXaa: 0.0 ± 0.0
Gln
4.306GlnAla: 4.306 ± 0.064
0.231GlnCys: 0.231 ± 0.014
1.592GlnAsp: 1.592 ± 0.043
1.544GlnGlu: 1.544 ± 0.044
1.192GlnPhe: 1.192 ± 0.036
2.633GlnGly: 2.633 ± 0.06
0.629GlnHis: 0.629 ± 0.028
1.76GlnIle: 1.76 ± 0.044
0.834GlnLys: 0.834 ± 0.029
3.345GlnLeu: 3.345 ± 0.061
0.893GlnMet: 0.893 ± 0.028
0.834GlnAsn: 0.834 ± 0.033
1.797GlnPro: 1.797 ± 0.044
1.243GlnGln: 1.243 ± 0.039
2.633GlnArg: 2.633 ± 0.06
1.833GlnSer: 1.833 ± 0.044
1.67GlnThr: 1.67 ± 0.048
2.39GlnVal: 2.39 ± 0.052
0.462GlnTrp: 0.462 ± 0.023
0.63GlnTyr: 0.63 ± 0.022
0.0GlnXaa: 0.0 ± 0.0
Arg
8.317ArgAla: 8.317 ± 0.112
0.543ArgCys: 0.543 ± 0.022
4.447ArgAsp: 4.447 ± 0.067
4.88ArgGlu: 4.88 ± 0.07
3.04ArgPhe: 3.04 ± 0.062
5.114ArgGly: 5.114 ± 0.081
1.557ArgHis: 1.557 ± 0.041
4.138ArgIle: 4.138 ± 0.055
2.248ArgLys: 2.248 ± 0.053
8.005ArgLeu: 8.005 ± 0.1
2.068ArgMet: 2.068 ± 0.049
1.899ArgAsn: 1.899 ± 0.046
3.417ArgPro: 3.417 ± 0.063
2.589ArgGln: 2.589 ± 0.058
5.443ArgArg: 5.443 ± 0.087
4.022ArgSer: 4.022 ± 0.07
3.456ArgThr: 3.456 ± 0.06
4.74ArgVal: 4.74 ± 0.077
1.24ArgTrp: 1.24 ± 0.043
1.713ArgTyr: 1.713 ± 0.05
0.0ArgXaa: 0.0 ± 0.0
Ser
6.317SerAla: 6.317 ± 0.087
0.385SerCys: 0.385 ± 0.017
3.39SerAsp: 3.39 ± 0.068
3.227SerGlu: 3.227 ± 0.061
2.237SerPhe: 2.237 ± 0.047
5.577SerGly: 5.577 ± 0.074
0.998SerHis: 0.998 ± 0.036
2.606SerIle: 2.606 ± 0.049
1.393SerLys: 1.393 ± 0.037
5.254SerLeu: 5.254 ± 0.073
1.253SerMet: 1.253 ± 0.037
1.355SerAsn: 1.355 ± 0.039
2.774SerPro: 2.774 ± 0.06
1.744SerGln: 1.744 ± 0.051
3.677SerArg: 3.677 ± 0.063
2.894SerSer: 2.894 ± 0.06
2.638SerThr: 2.638 ± 0.056
3.759SerVal: 3.759 ± 0.059
0.784SerTrp: 0.784 ± 0.025
1.316SerTyr: 1.316 ± 0.037
0.0SerXaa: 0.0 ± 0.0
Thr
6.256ThrAla: 6.256 ± 0.088
0.386ThrCys: 0.386 ± 0.019
2.932ThrAsp: 2.932 ± 0.056
2.714ThrGlu: 2.714 ± 0.054
1.766ThrPhe: 1.766 ± 0.042
5.353ThrGly: 5.353 ± 0.076
0.941ThrHis: 0.941 ± 0.032
2.922ThrIle: 2.922 ± 0.054
1.342ThrLys: 1.342 ± 0.038
5.616ThrLeu: 5.616 ± 0.075
1.278ThrMet: 1.278 ± 0.034
1.309ThrAsn: 1.309 ± 0.039
3.103ThrPro: 3.103 ± 0.071
1.634ThrGln: 1.634 ± 0.044
3.488ThrArg: 3.488 ± 0.064
2.683ThrSer: 2.683 ± 0.056
2.676ThrThr: 2.676 ± 0.057
3.985ThrVal: 3.985 ± 0.063
0.549ThrTrp: 0.549 ± 0.025
1.165ThrTyr: 1.165 ± 0.041
0.0ThrXaa: 0.0 ± 0.0
Val
9.125ValAla: 9.125 ± 0.102
0.536ValCys: 0.536 ± 0.022
4.193ValAsp: 4.193 ± 0.066
4.962ValGlu: 4.962 ± 0.083
2.451ValPhe: 2.451 ± 0.054
5.634ValGly: 5.634 ± 0.084
1.279ValHis: 1.279 ± 0.039
3.646ValIle: 3.646 ± 0.066
1.841ValLys: 1.841 ± 0.047
6.875ValLeu: 6.875 ± 0.095
1.71ValMet: 1.71 ± 0.045
1.828ValAsn: 1.828 ± 0.045
3.687ValPro: 3.687 ± 0.06
1.941ValGln: 1.941 ± 0.046
4.801ValArg: 4.801 ± 0.067
3.877ValSer: 3.877 ± 0.063
4.184ValThr: 4.184 ± 0.065
5.272ValVal: 5.272 ± 0.074
0.914ValTrp: 0.914 ± 0.033
1.277ValTyr: 1.277 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
1.292TrpAla: 1.292 ± 0.046
0.12TrpCys: 0.12 ± 0.011
0.721TrpAsp: 0.721 ± 0.026
0.669TrpGlu: 0.669 ± 0.027
0.62TrpPhe: 0.62 ± 0.028
0.94TrpGly: 0.94 ± 0.033
0.356TrpHis: 0.356 ± 0.02
0.708TrpIle: 0.708 ± 0.029
0.408TrpLys: 0.408 ± 0.021
1.809TrpLeu: 1.809 ± 0.051
0.35TrpMet: 0.35 ± 0.018
0.428TrpAsn: 0.428 ± 0.02
0.652TrpPro: 0.652 ± 0.025
0.676TrpGln: 0.676 ± 0.029
1.236TrpArg: 1.236 ± 0.038
0.898TrpSer: 0.898 ± 0.032
0.768TrpThr: 0.768 ± 0.028
0.852TrpVal: 0.852 ± 0.033
0.244TrpTrp: 0.244 ± 0.018
0.268TrpTyr: 0.268 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.451TyrAla: 2.451 ± 0.047
0.229TyrCys: 0.229 ± 0.016
1.545TyrAsp: 1.545 ± 0.037
1.295TyrGlu: 1.295 ± 0.041
0.888TyrPhe: 0.888 ± 0.034
2.077TyrGly: 2.077 ± 0.044
0.455TyrHis: 0.455 ± 0.019
0.867TyrIle: 0.867 ± 0.032
0.468TyrLys: 0.468 ± 0.023
2.14TyrLeu: 2.14 ± 0.043
0.363TyrMet: 0.363 ± 0.018
0.56TyrAsn: 0.56 ± 0.027
1.015TyrPro: 1.015 ± 0.038
0.653TyrGln: 0.653 ± 0.028
1.868TyrArg: 1.868 ± 0.048
1.267TyrSer: 1.267 ± 0.039
1.021TyrThr: 1.021 ± 0.039
1.384TyrVal: 1.384 ± 0.043
0.311TyrTrp: 0.311 ± 0.019
0.583TyrTyr: 0.583 ± 0.026
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3036 proteins (946070 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski