Hanseniaspora osmophila
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4653 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E5RYQ3|A0A1E5RYQ3_9ASCO Prefoldin subunit 5 OS=Hanseniaspora osmophila OX=56408 GN=AWRI3579_g252 PE=4 SV=1
MM1 pKa = 7.8 ASTISVKK8 pKa = 10.74 DD9 pKa = 3.41 QIDD12 pKa = 3.26 PRR14 pKa = 11.84 ACVLQAKK21 pKa = 8.19 EE22 pKa = 3.98 ALQDD26 pKa = 3.54 SSHH29 pKa = 6.82 AEE31 pKa = 3.8 NDD33 pKa = 3.42 RR34 pKa = 11.84 QLQGTTICEE43 pKa = 3.76 EE44 pKa = 4.42 DD45 pKa = 3.41 GLYY48 pKa = 10.22 QYY50 pKa = 10.72 EE51 pKa = 4.37 DD52 pKa = 3.65 EE53 pKa = 5.08 EE54 pKa = 4.58 MPPGCISVKK63 pKa = 10.53 DD64 pKa = 3.63 FAYY67 pKa = 9.65 TLEE70 pKa = 5.03 DD71 pKa = 3.67 PLHH74 pKa = 5.99 YY75 pKa = 10.46 GFYY78 pKa = 10.5 SDD80 pKa = 3.44 TEE82 pKa = 4.35 EE83 pKa = 4.53 YY84 pKa = 10.85 DD85 pKa = 3.37 EE86 pKa = 4.24 EE87 pKa = 6.08 AYY89 pKa = 10.49 GGEE92 pKa = 3.74 SDD94 pKa = 5.42 EE95 pKa = 4.85 YY96 pKa = 11.4 DD97 pKa = 3.25 EE98 pKa = 5.67 HH99 pKa = 7.93 DD100 pKa = 4.5 PNSVLQEE107 pKa = 4.12 YY108 pKa = 10.06 YY109 pKa = 10.83 EE110 pKa = 4.76 NDD112 pKa = 4.0 DD113 pKa = 4.99 DD114 pKa = 6.94 DD115 pKa = 5.3 EE116 pKa = 7.28 DD117 pKa = 4.36 EE118 pKa = 4.83 DD119 pKa = 4.72 NKK121 pKa = 9.94 TNHH124 pKa = 5.45 YY125 pKa = 9.8 QEE127 pKa = 5.35 HH128 pKa = 6.59 DD129 pKa = 3.3 QGNEE133 pKa = 3.71 YY134 pKa = 10.87 SQDD137 pKa = 3.12 KK138 pKa = 10.58 YY139 pKa = 11.41 FEE141 pKa = 4.7 DD142 pKa = 3.8 FDD144 pKa = 4.02 RR145 pKa = 11.84 RR146 pKa = 11.84 KK147 pKa = 10.41 SYY149 pKa = 10.85 IMPSEE154 pKa = 4.33 HH155 pKa = 6.72 IINRR159 pKa = 11.84 KK160 pKa = 9.52 AIALYY165 pKa = 10.52 DD166 pKa = 3.63 FEE168 pKa = 5.61 NEE170 pKa = 4.11 NEE172 pKa = 4.16 NEE174 pKa = 4.16 LGLQEE179 pKa = 4.18 GQVVFIDD186 pKa = 3.77 YY187 pKa = 10.61 RR188 pKa = 11.84 HH189 pKa = 5.17 GQGWLVVEE197 pKa = 4.92 TMDD200 pKa = 5.1 GMHH203 pKa = 6.78 TGLVPEE209 pKa = 5.77 DD210 pKa = 3.52 YY211 pKa = 11.04 LQILDD216 pKa = 4.32 EE217 pKa = 4.63 GEE219 pKa = 3.94 EE220 pKa = 4.47 DD221 pKa = 3.53 EE222 pKa = 5.91 DD223 pKa = 5.78 ADD225 pKa = 4.55 AEE227 pKa = 4.3 AHH229 pKa = 6.31 NGFANEE235 pKa = 4.11 YY236 pKa = 10.89 ADD238 pKa = 3.5 ATINNTVVNNTLDD251 pKa = 3.67 EE252 pKa = 4.22 THH254 pKa = 7.01 EE255 pKa = 4.65 DD256 pKa = 3.57 CAKK259 pKa = 10.67 RR260 pKa = 11.84 PDD262 pKa = 3.84 YY263 pKa = 10.9 LAGLFDD269 pKa = 4.16 VEE271 pKa = 4.72 QGTGPLPSNGNNYY284 pKa = 9.94 SSHH287 pKa = 6.46 TSLTDD292 pKa = 3.7 PLNEE296 pKa = 4.22 DD297 pKa = 3.97 LKK299 pKa = 10.31 TLSIHH304 pKa = 6.58
Molecular weight: 34.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.777
IPC2_protein 3.859
IPC_protein 3.872
Toseland 3.668
ProMoST 3.999
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.745
Rodwell 3.694
Grimsley 3.579
Solomon 3.834
Lehninger 3.795
Nozaki 3.948
DTASelect 4.151
Thurlkill 3.706
EMBOSS 3.757
Sillero 3.986
Patrickios 0.617
IPC_peptide 3.834
IPC2_peptide 3.973
IPC2.peptide.svr19 3.864
Protein with the highest isoelectric point:
>tr|A0A1E5RGH0|A0A1E5RGH0_9ASCO Uncharacterized protein OS=Hanseniaspora osmophila OX=56408 GN=AWRI3579_g1086 PE=4 SV=1
MM1 pKa = 7.69 NMIIQRR7 pKa = 11.84 VGPQVRR13 pKa = 11.84 DD14 pKa = 3.25 NSQVFGVARR23 pKa = 11.84 IYY25 pKa = 11.32 ASFNDD30 pKa = 4.2 TFVHH34 pKa = 5.59 VTDD37 pKa = 4.97 LSGKK41 pKa = 7.19 EE42 pKa = 4.09 TICRR46 pKa = 11.84 VTGGMKK52 pKa = 10.15 VKK54 pKa = 10.61 ADD56 pKa = 4.11 RR57 pKa = 11.84 DD58 pKa = 3.64 EE59 pKa = 4.65 SSPYY63 pKa = 11.08 AAMLAAQDD71 pKa = 3.41 VAVRR75 pKa = 11.84 CKK77 pKa = 10.53 EE78 pKa = 3.66 IGITAVHH85 pKa = 5.5 VKK87 pKa = 9.98 IRR89 pKa = 11.84 GTGGTRR95 pKa = 11.84 SKK97 pKa = 10.09 TPGPGGQSALRR108 pKa = 11.84 ALARR112 pKa = 11.84 SGLRR116 pKa = 11.84 IGRR119 pKa = 11.84 IEE121 pKa = 4.8 DD122 pKa = 3.41 VTPIPSDD129 pKa = 3.39 STRR132 pKa = 11.84 KK133 pKa = 9.52 KK134 pKa = 8.91 GGRR137 pKa = 11.84 RR138 pKa = 11.84 GRR140 pKa = 11.84 RR141 pKa = 11.84 LL142 pKa = 3.07
Molecular weight: 15.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.318
IPC2_protein 9.721
IPC_protein 10.76
Toseland 11.038
ProMoST 11.038
Dawson 11.082
Bjellqvist 10.877
Wikipedia 11.374
Rodwell 11.184
Grimsley 11.125
Solomon 11.345
Lehninger 11.286
Nozaki 11.038
DTASelect 10.877
Thurlkill 11.038
EMBOSS 11.477
Sillero 11.038
Patrickios 10.921
IPC_peptide 11.359
IPC2_peptide 10.101
IPC2.peptide.svr19 8.844
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4653
0
4653
2567851
66
4918
551.9
62.05
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.478 ± 0.027
1.238 ± 0.013
5.709 ± 0.025
6.534 ± 0.036
4.515 ± 0.024
4.893 ± 0.03
2.206 ± 0.013
5.868 ± 0.028
7.771 ± 0.036
9.228 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.114 ± 0.013
6.874 ± 0.043
4.066 ± 0.025
4.477 ± 0.03
3.466 ± 0.021
9.203 ± 0.059
6.122 ± 0.042
5.574 ± 0.026
0.875 ± 0.009
3.465 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here