Tropicimonas isoalkanivorans

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Tropicimonas

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4790 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I1E176|A0A1I1E176_9RHOB Bifunctional NAD(P)H-hydrate repair enzyme OS=Tropicimonas isoalkanivorans OX=441112 GN=nnrD PE=3 SV=1
MM1 pKa = 7.18VKK3 pKa = 10.28INIANTAGTGLIMSPDD19 pKa = 3.84DD20 pKa = 3.85ALQFATNVPAASDD33 pKa = 3.95YY34 pKa = 10.88EE35 pKa = 4.56KK36 pKa = 11.14VSSTVLSTDD45 pKa = 3.44PEE47 pKa = 4.42NPNYY51 pKa = 9.44MDD53 pKa = 3.52VVATLSTGIDD63 pKa = 3.24APFQDD68 pKa = 3.25VEE70 pKa = 4.35AYY72 pKa = 10.54YY73 pKa = 10.57DD74 pKa = 3.93YY75 pKa = 11.42DD76 pKa = 4.06PEE78 pKa = 4.24TTEE81 pKa = 3.66YY82 pKa = 10.63HH83 pKa = 5.05LTGLVFSNADD93 pKa = 3.08GVTTLTLTDD102 pKa = 4.9LYY104 pKa = 10.86TPATEE109 pKa = 4.38AEE111 pKa = 4.59VNAEE115 pKa = 4.19TIDD118 pKa = 3.73FGDD121 pKa = 3.61FFEE124 pKa = 5.18GRR126 pKa = 11.84DD127 pKa = 3.67VIHH130 pKa = 6.74GGKK133 pKa = 9.89FDD135 pKa = 4.04DD136 pKa = 4.31VLYY139 pKa = 9.92GQQRR143 pKa = 11.84GDD145 pKa = 3.07VLYY148 pKa = 10.2GHH150 pKa = 7.39RR151 pKa = 11.84GNDD154 pKa = 3.8EE155 pKa = 3.68IHH157 pKa = 6.58GGAGSDD163 pKa = 3.62MLAGGYY169 pKa = 9.83GADD172 pKa = 3.51TLFGGTGDD180 pKa = 4.99DD181 pKa = 3.81LLKK184 pKa = 10.94GQVGRR189 pKa = 11.84DD190 pKa = 3.35TLYY193 pKa = 11.18GGGSNDD199 pKa = 3.08ILRR202 pKa = 11.84GGWGQDD208 pKa = 2.98TLYY211 pKa = 11.24GGAGYY216 pKa = 10.39DD217 pKa = 3.66YY218 pKa = 10.98LYY220 pKa = 10.61GGPGADD226 pKa = 2.2TFVFFSGDD234 pKa = 3.26GVAEE238 pKa = 4.44IYY240 pKa = 10.41RR241 pKa = 11.84FRR243 pKa = 11.84DD244 pKa = 3.33GRR246 pKa = 11.84DD247 pKa = 2.99HH248 pKa = 6.46VQIEE252 pKa = 4.41SGAEE256 pKa = 3.83SFDD259 pKa = 4.16DD260 pKa = 4.44VEE262 pKa = 4.38ITQDD266 pKa = 3.22HH267 pKa = 7.03ADD269 pKa = 3.46AVATFDD275 pKa = 3.81DD276 pKa = 3.88VTIIFLDD283 pKa = 3.43TDD285 pKa = 3.9ASVLGADD292 pKa = 4.71DD293 pKa = 5.76FIFF296 pKa = 4.54

Molecular weight: 31.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I1RBE9|A0A1I1RBE9_9RHOB Putative transposase OS=Tropicimonas isoalkanivorans OX=441112 GN=SAMN04488094_1261 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4790

0

4790

1477901

39

2946

308.5

33.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.385 ± 0.044

0.89 ± 0.011

6.12 ± 0.034

6.169 ± 0.036

3.666 ± 0.025

8.826 ± 0.049

2.012 ± 0.016

4.979 ± 0.023

2.86 ± 0.026

9.928 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.646 ± 0.018

2.443 ± 0.021

5.237 ± 0.028

2.934 ± 0.019

7.128 ± 0.041

5.199 ± 0.027

5.518 ± 0.023

7.446 ± 0.029

1.429 ± 0.015

2.183 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski