Beauveria bassiana (White muscardine disease fungus) (Tritirachium shiotae)
Average proteome isoelectric point is 6.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 10656 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N6NWK6|A0A2N6NWK6_BEABA Uncharacterized protein OS=Beauveria bassiana OX=176275 GN=BM221_001757 PE=4 SV=1
MM1 pKa = 7.32 SNYY4 pKa = 10.16 NGRR7 pKa = 11.84 HH8 pKa = 5.09 GPNVANYY15 pKa = 10.07 LRR17 pKa = 11.84 DD18 pKa = 3.73 LNTISPQEE26 pKa = 3.9 GSVDD30 pKa = 3.38 EE31 pKa = 4.36 NFNIEE36 pKa = 3.76 GDD38 pKa = 3.58 LALFTNTEE46 pKa = 3.91 FFDD49 pKa = 4.93 FEE51 pKa = 4.58 TGQNTDD57 pKa = 3.67 FQAQPVKK64 pKa = 10.15 EE65 pKa = 4.36 ATHH68 pKa = 5.75 STPSSDD74 pKa = 4.38 DD75 pKa = 3.21 VSAAPSVMGDD85 pKa = 3.4 MPNLDD90 pKa = 5.16 FISSTYY96 pKa = 10.27 IFFYY100 pKa = 10.89 VLFYY104 pKa = 10.61 FPP106 pKa = 5.4
Molecular weight: 11.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.897
Patrickios 0.769
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|A0A2N6NLC1|A0A2N6NLC1_BEABA Cation-transporting ATPase OS=Beauveria bassiana OX=176275 GN=BM221_006212 PE=3 SV=1
MM1 pKa = 7.27 SAVKK5 pKa = 10.14 AAWTPYY11 pKa = 9.16 SQSASSPPPPAPPAPSPSSQHH32 pKa = 4.95 SASRR36 pKa = 11.84 IAAMEE41 pKa = 3.94 AVAPAHH47 pKa = 6.5 RR48 pKa = 11.84 AGSAHH53 pKa = 6.74 AADD56 pKa = 4.26 TASVPATGGGRR67 pKa = 11.84 CSSSTKK73 pKa = 10.32 LLYY76 pKa = 10.13 VRR78 pKa = 11.84 CSVALHH84 pKa = 7.04 DD85 pKa = 4.15 GAARR89 pKa = 11.84 PRR91 pKa = 11.84 VAADD95 pKa = 3.43 GLSAGGSCTRR105 pKa = 11.84 KK106 pKa = 9.56 AARR109 pKa = 11.84 ASSARR114 pKa = 11.84 RR115 pKa = 11.84 DD116 pKa = 3.27 AATRR120 pKa = 11.84 SCVEE124 pKa = 3.71 EE125 pKa = 4.89 RR126 pKa = 11.84 SAQRR130 pKa = 11.84 DD131 pKa = 3.22 RR132 pKa = 11.84 MAEE135 pKa = 3.61 AKK137 pKa = 8.9 MAQSLACCMAKK148 pKa = 8.95 STWAIHH154 pKa = 5.16 QSLVHH159 pKa = 6.01 SRR161 pKa = 11.84 RR162 pKa = 11.84 CTGSILEE169 pKa = 4.62 CVASRR174 pKa = 11.84 CMPWPMAMRR183 pKa = 11.84 IAKK186 pKa = 9.16 PIQCHH191 pKa = 5.4 AFATISSCPSEE202 pKa = 3.93 YY203 pKa = 10.4 HH204 pKa = 6.35 VISSATCTYY213 pKa = 9.32 MHH215 pKa = 6.49 MHH217 pKa = 7.24 AHH219 pKa = 4.58 QQHH222 pKa = 7.14 RR223 pKa = 11.84 IQQRR227 pKa = 11.84 QHH229 pKa = 4.7 EE230 pKa = 4.61 RR231 pKa = 11.84 VPRR234 pKa = 11.84 VAAAVLRR241 pKa = 11.84 HH242 pKa = 5.7 KK243 pKa = 10.89 VLLVHH248 pKa = 6.25 NRR250 pKa = 11.84 PSRR253 pKa = 11.84 VHH255 pKa = 5.46 AVKK258 pKa = 10.8 VGAQQAALEE267 pKa = 4.23 RR268 pKa = 11.84 RR269 pKa = 11.84 GAPRR273 pKa = 11.84 QGLGRR278 pKa = 11.84 HH279 pKa = 5.63 ALQLVRR285 pKa = 11.84 RR286 pKa = 11.84 VAPRR290 pKa = 11.84 KK291 pKa = 9.17 LAAAKK296 pKa = 10.04 VKK298 pKa = 10.44 GVASLRR304 pKa = 11.84 LRR306 pKa = 11.84 DD307 pKa = 4.59 LPHH310 pKa = 5.87 QVAKK314 pKa = 10.15 VGHH317 pKa = 5.53 VAADD321 pKa = 3.56 EE322 pKa = 4.01 AHH324 pKa = 6.43 AAEE327 pKa = 4.53 LPEE330 pKa = 5.16 LGVAQVLPVDD340 pKa = 3.95 EE341 pKa = 4.72 VGQVAGLMFLVLCC354 pKa = 5.17
Molecular weight: 37.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.353
IPC2_protein 9.458
IPC_protein 10.087
Toseland 10.599
ProMoST 10.277
Dawson 10.687
Bjellqvist 10.423
Wikipedia 10.891
Rodwell 10.862
Grimsley 10.73
Solomon 10.818
Lehninger 10.789
Nozaki 10.657
DTASelect 10.394
Thurlkill 10.599
EMBOSS 11.008
Sillero 10.643
Patrickios 10.57
IPC_peptide 10.818
IPC2_peptide 9.838
IPC2.peptide.svr19 8.552
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
10656
0
10656
4698728
19
4917
440.9
48.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.732 ± 0.025
1.223 ± 0.008
5.93 ± 0.017
5.937 ± 0.021
3.579 ± 0.016
7.043 ± 0.025
2.409 ± 0.012
4.58 ± 0.018
4.7 ± 0.021
8.89 ± 0.025
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.244 ± 0.009
3.482 ± 0.013
5.812 ± 0.026
4.027 ± 0.017
6.307 ± 0.024
7.936 ± 0.03
5.841 ± 0.017
6.293 ± 0.018
1.423 ± 0.008
2.612 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here