Saliniradius amylolyticus
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2945 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2S2E1X4|A0A2S2E1X4_9ALTE tRNA pseudouridine synthase D OS=Saliniradius amylolyticus OX=2183582 GN=truD PE=3 SV=1
MM1 pKa = 7.79 KK2 pKa = 10.42 SITPILAALTLPFVLTGCLNSDD24 pKa = 3.81 SDD26 pKa = 4.59 PLPEE30 pKa = 4.82 PPEE33 pKa = 4.15 PPAFSNLRR41 pKa = 11.84 VTHH44 pKa = 6.33 AVSDD48 pKa = 3.96 APTVNVIANGDD59 pKa = 4.05 TLSEE63 pKa = 4.22 GADD66 pKa = 3.74 YY67 pKa = 10.63 QASTGWQSVLSDD79 pKa = 3.86 TYY81 pKa = 10.32 TVEE84 pKa = 4.27 VNANLPGGDD93 pKa = 3.55 SATVIGPVDD102 pKa = 3.77 LTLTGDD108 pKa = 3.31 MNYY111 pKa = 10.86 DD112 pKa = 3.41 VVAVGQVGDD121 pKa = 3.93 IEE123 pKa = 4.38 PLVLEE128 pKa = 4.29 NAEE131 pKa = 4.31 TTVTSGNARR140 pKa = 11.84 VQILHH145 pKa = 6.81 AAANAPMVDD154 pKa = 3.63 IYY156 pKa = 10.59 VTAPDD161 pKa = 4.11 AEE163 pKa = 4.48 LASEE167 pKa = 4.07 QALATLSFKK176 pKa = 10.72 EE177 pKa = 4.1 YY178 pKa = 9.37 TAQTEE183 pKa = 4.58 VAGGDD188 pKa = 3.32 YY189 pKa = 10.17 RR190 pKa = 11.84 VRR192 pKa = 11.84 ITPAGADD199 pKa = 3.1 TVVFDD204 pKa = 4.87 SGTINLADD212 pKa = 4.27 GADD215 pKa = 3.73 LMVAATDD222 pKa = 3.5 NTGAGDD228 pKa = 3.99 SPVTLLAVDD237 pKa = 3.97 ANGATRR243 pKa = 11.84 LWDD246 pKa = 3.7 TNTGANLRR254 pKa = 11.84 VLHH257 pKa = 6.52 GSPDD261 pKa = 3.44 APAVDD266 pKa = 3.46 VLVNNATMPMHH277 pKa = 5.85 YY278 pKa = 9.99 QLDD281 pKa = 3.73 GLAYY285 pKa = 9.81 PNATDD290 pKa = 3.9 YY291 pKa = 11.51 LAVPADD297 pKa = 3.99 EE298 pKa = 5.12 LLVDD302 pKa = 4.25 VVADD306 pKa = 3.74 ADD308 pKa = 3.91 NSVVAIDD315 pKa = 4.33 DD316 pKa = 3.87 AAVAPMQGMAYY327 pKa = 8.59 TAIATNTLADD337 pKa = 3.52 IQLALLTDD345 pKa = 3.72 DD346 pKa = 4.43 HH347 pKa = 7.79 RR348 pKa = 11.84 SIATAAKK355 pKa = 9.92 VRR357 pKa = 11.84 LIHH360 pKa = 6.13 NAPAAGDD367 pKa = 3.29 VDD369 pKa = 3.95 IYY371 pKa = 10.52 VTADD375 pKa = 3.79 DD376 pKa = 5.98 DD377 pKa = 5.31 ISDD380 pKa = 5.09 DD381 pKa = 4.48 DD382 pKa = 4.34 PAFTDD387 pKa = 3.6 VPYY390 pKa = 10.91 DD391 pKa = 3.59 ATNLATTGYY400 pKa = 10.37 VEE402 pKa = 4.27 LAEE405 pKa = 4.03 GTYY408 pKa = 10.73 YY409 pKa = 9.31 ITITPADD416 pKa = 4.06 TKK418 pKa = 10.76 TEE420 pKa = 4.68 AIGPLMVEE428 pKa = 4.59 LNNGDD433 pKa = 3.76 VLSAVALEE441 pKa = 4.32 ANGGGTPLQAVILDD455 pKa = 3.93 DD456 pKa = 4.33 SKK458 pKa = 10.97 PAPVSLDD465 pKa = 3.02 ATQTFEE471 pKa = 4.04 VSLDD475 pKa = 3.47 GMQEE479 pKa = 3.94 VPAVDD484 pKa = 3.85 TAATAAATVLLDD496 pKa = 3.6 EE497 pKa = 5.44 DD498 pKa = 4.66 DD499 pKa = 4.66 RR500 pKa = 11.84 LFSVTVDD507 pKa = 3.21 TSAVDD512 pKa = 3.39 NVTGVHH518 pKa = 4.92 VHH520 pKa = 7.22 DD521 pKa = 5.15 GNIGRR526 pKa = 11.84 NGPVAFPLQASGDD539 pKa = 3.8 DD540 pKa = 4.3 TYY542 pKa = 11.62 TLPATNLLDD551 pKa = 4.22 PMIEE555 pKa = 3.97 ALKK558 pKa = 10.59 SGEE561 pKa = 3.88 WYY563 pKa = 10.22 INVHH567 pKa = 5.27 TEE569 pKa = 3.88 ANPSGEE575 pKa = 4.01 VRR577 pKa = 11.84 GQIVPEE583 pKa = 4.05 TMAVVTFPLSGSQEE597 pKa = 4.35 VPSVTTDD604 pKa = 2.63 AMGYY608 pKa = 10.59 GYY610 pKa = 9.02 ATLDD614 pKa = 3.55 TTTLAVDD621 pKa = 4.09 LVAVTMGVEE630 pKa = 4.34 DD631 pKa = 3.3 ATMAHH636 pKa = 6.57 IHH638 pKa = 5.92 TGYY641 pKa = 10.6 AGEE644 pKa = 4.34 NGPVYY649 pKa = 9.8 VTLEE653 pKa = 3.81 QSMDD657 pKa = 3.82 DD658 pKa = 3.59 ANVWMTPAGAMIDD671 pKa = 3.54 ATNAARR677 pKa = 11.84 LVEE680 pKa = 4.37 GGHH683 pKa = 5.74 YY684 pKa = 10.93 VNIHH688 pKa = 5.45 TPANPSGEE696 pKa = 4.24 LRR698 pKa = 11.84 GQITPDD704 pKa = 3.38 NIEE707 pKa = 4.21 VYY709 pKa = 10.57 GVTATGDD716 pKa = 3.59 QEE718 pKa = 4.69 VPAVDD723 pKa = 3.78 TNAMGEE729 pKa = 4.25 GAITLNTSTMTIKK742 pKa = 10.87 AIINVMDD749 pKa = 4.07 ISPNAAHH756 pKa = 7.0 IHH758 pKa = 5.03 QGAVGEE764 pKa = 4.21 NGGVVIGLTNPDD776 pKa = 3.33 AGLWTLDD783 pKa = 3.25 ATLTEE788 pKa = 4.16 AQMALMQAEE797 pKa = 4.01 GLYY800 pKa = 10.82 TNFHH804 pKa = 6.36 TDD806 pKa = 3.12 AFPSGEE812 pKa = 3.71 IRR814 pKa = 11.84 GQITLGFEE822 pKa = 4.07
Molecular weight: 85.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.684
IPC2_protein 3.656
IPC_protein 3.706
Toseland 3.478
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.872
Wikipedia 3.643
Rodwell 3.528
Grimsley 3.376
Solomon 3.706
Lehninger 3.656
Nozaki 3.808
DTASelect 4.088
Thurlkill 3.528
EMBOSS 3.656
Sillero 3.834
Patrickios 1.087
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.737
Protein with the highest isoelectric point:
>tr|A0A2S2E7F6|A0A2S2E7F6_9ALTE OMP_b-brl domain-containing protein OS=Saliniradius amylolyticus OX=2183582 GN=HMF8227_02709 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNIKK11 pKa = 10.11 RR12 pKa = 11.84 KK13 pKa = 9.04 RR14 pKa = 11.84 SHH16 pKa = 6.18 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.42 NGRR28 pKa = 11.84 KK29 pKa = 8.55 VLKK32 pKa = 10.18 SRR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.31 GRR39 pKa = 11.84 ARR41 pKa = 11.84 LAAA44 pKa = 4.44
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.705
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.427
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.142
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2945
0
2945
972597
30
1835
330.3
36.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.898 ± 0.046
0.945 ± 0.017
5.918 ± 0.039
6.405 ± 0.046
3.831 ± 0.03
7.162 ± 0.038
2.397 ± 0.021
5.357 ± 0.032
4.535 ± 0.039
10.685 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.567 ± 0.022
3.723 ± 0.027
4.242 ± 0.03
5.368 ± 0.043
5.376 ± 0.036
6.203 ± 0.034
5.038 ± 0.03
6.946 ± 0.039
1.34 ± 0.019
3.065 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here