Passion fruit chlorotic mottle virus
Average proteome isoelectric point is 7.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2R4Q8V4|A0A2R4Q8V4_9GEMI Replication-associated protein OS=Passion fruit chlorotic mottle virus OX=2162638 PE=3 SV=1
MM1 pKa = 7.52 SISSEE6 pKa = 3.5 IFEE9 pKa = 4.66 EE10 pKa = 3.98 IKK12 pKa = 10.46 EE13 pKa = 4.25 NKK15 pKa = 9.73 VSITVFFIIFFCVSVINVVCCAFGFWQSAGPRR47 pKa = 11.84 GGAVVYY53 pKa = 10.82 VGGEE57 pKa = 4.09 AINDD61 pKa = 3.89 GGEE64 pKa = 4.3 EE65 pKa = 3.84 ICSGKK70 pKa = 10.06 KK71 pKa = 9.6 AQPGG75 pKa = 3.34
Molecular weight: 7.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.632
IPC2_protein 4.863
IPC_protein 4.584
Toseland 4.457
ProMoST 4.647
Dawson 4.495
Bjellqvist 4.647
Wikipedia 4.317
Rodwell 4.431
Grimsley 4.368
Solomon 4.482
Lehninger 4.431
Nozaki 4.609
DTASelect 4.635
Thurlkill 4.444
EMBOSS 4.329
Sillero 4.673
Patrickios 3.3
IPC_peptide 4.495
IPC2_peptide 4.673
IPC2.peptide.svr19 4.672
Protein with the highest isoelectric point:
>tr|A0A2R4Q8V2|A0A2R4Q8V2_9GEMI Movement protein BC1 OS=Passion fruit chlorotic mottle virus OX=2162638 PE=3 SV=1
MM1 pKa = 6.44 VTTRR5 pKa = 11.84 SGRR8 pKa = 11.84 TYY10 pKa = 10.96 GPPTAQTRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 FPPMRR25 pKa = 11.84 RR26 pKa = 11.84 RR27 pKa = 11.84 PAMDD31 pKa = 3.42 RR32 pKa = 11.84 VIGPSKK38 pKa = 9.92 KK39 pKa = 7.95 TVKK42 pKa = 10.17 RR43 pKa = 11.84 MKK45 pKa = 9.71 KK46 pKa = 9.09 GKK48 pKa = 10.12 SGGIPVGCKK57 pKa = 10.25 GPCKK61 pKa = 9.43 THH63 pKa = 5.36 TVDD66 pKa = 4.55 VIATVTHH73 pKa = 6.94 DD74 pKa = 3.49 GKK76 pKa = 11.54 GPGLVSNISKK86 pKa = 10.87 GDD88 pKa = 3.58 DD89 pKa = 3.1 FGQRR93 pKa = 11.84 EE94 pKa = 4.14 GRR96 pKa = 11.84 RR97 pKa = 11.84 IRR99 pKa = 11.84 VTKK102 pKa = 10.48 LLLRR106 pKa = 11.84 GKK108 pKa = 9.77 VWLPQDD114 pKa = 3.25 KK115 pKa = 9.57 ATIAGSNIMRR125 pKa = 11.84 LWVMKK130 pKa = 10.18 DD131 pKa = 3.08 RR132 pKa = 11.84 RR133 pKa = 11.84 PGSQHH138 pKa = 4.92 VAFEE142 pKa = 4.47 ALFDD146 pKa = 3.97 MADD149 pKa = 4.06 KK150 pKa = 10.68 EE151 pKa = 4.29 PSTALVKK158 pKa = 9.75 MDD160 pKa = 3.2 YY161 pKa = 10.03 RR162 pKa = 11.84 DD163 pKa = 3.33 RR164 pKa = 11.84 FIVIKK169 pKa = 10.75 DD170 pKa = 3.48 MEE172 pKa = 6.08 IDD174 pKa = 3.4 LHH176 pKa = 6.29 GGRR179 pKa = 11.84 DD180 pKa = 3.71 FRR182 pKa = 11.84 VDD184 pKa = 3.43 EE185 pKa = 4.26 EE186 pKa = 4.73 TFDD189 pKa = 3.27 IMVPINCDD197 pKa = 3.07 VLFDD201 pKa = 4.75 HH202 pKa = 7.1 NDD204 pKa = 3.44 EE205 pKa = 4.8 GSLTTTLEE213 pKa = 3.72 NGIIVYY219 pKa = 9.53 YY220 pKa = 10.78 AVTDD224 pKa = 4.07 PAQVMQLTAQCRR236 pKa = 11.84 LYY238 pKa = 11.08 FFDD241 pKa = 3.86 STSNN245 pKa = 3.26
Molecular weight: 27.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.991
IPC2_protein 8.902
IPC_protein 8.873
Toseland 9.78
ProMoST 9.414
Dawson 9.955
Bjellqvist 9.633
Wikipedia 10.087
Rodwell 10.365
Grimsley 9.999
Solomon 9.999
Lehninger 9.97
Nozaki 9.867
DTASelect 9.604
Thurlkill 9.823
EMBOSS 10.175
Sillero 9.897
Patrickios 9.911
IPC_peptide 9.999
IPC2_peptide 8.273
IPC2.peptide.svr19 7.839
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
1335
75
319
222.5
25.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.318 ± 0.604
2.397 ± 0.279
7.116 ± 0.713
5.169 ± 0.649
6.367 ± 0.983
4.944 ± 1.263
2.996 ± 0.352
6.891 ± 0.599
5.693 ± 0.914
5.843 ± 0.723
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.221 ± 0.348
4.419 ± 0.48
6.067 ± 0.767
2.472 ± 0.319
7.865 ± 0.973
7.566 ± 1.001
5.019 ± 0.929
5.993 ± 1.053
1.723 ± 0.363
2.921 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here