Amino acid dipepetide frequency for Pantoea phage vB_PagM_PSKM

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.342AlaAla: 10.342 ± 1.248
1.113AlaCys: 1.113 ± 0.266
5.891AlaAsp: 5.891 ± 0.563
7.2AlaGlu: 7.2 ± 0.969
3.077AlaPhe: 3.077 ± 0.495
8.771AlaGly: 8.771 ± 0.673
1.44AlaHis: 1.44 ± 0.423
6.153AlaIle: 6.153 ± 0.626
5.04AlaLys: 5.04 ± 0.686
7.462AlaLeu: 7.462 ± 0.653
3.011AlaMet: 3.011 ± 0.403
3.666AlaAsn: 3.666 ± 0.529
2.356AlaPro: 2.356 ± 0.382
3.338AlaGln: 3.338 ± 0.36
4.255AlaArg: 4.255 ± 0.642
6.153AlaSer: 6.153 ± 0.823
4.517AlaThr: 4.517 ± 0.675
6.218AlaVal: 6.218 ± 0.675
1.375AlaTrp: 1.375 ± 0.405
3.142AlaTyr: 3.142 ± 0.471
0.0AlaXaa: 0.0 ± 0.0
Cys
0.72CysAla: 0.72 ± 0.211
0.393CysCys: 0.393 ± 0.152
0.131CysAsp: 0.131 ± 0.102
1.047CysGlu: 1.047 ± 0.35
0.458CysPhe: 0.458 ± 0.219
0.785CysGly: 0.785 ± 0.229
0.524CysHis: 0.524 ± 0.213
1.113CysIle: 1.113 ± 0.358
0.393CysLys: 0.393 ± 0.179
0.785CysLeu: 0.785 ± 0.2
0.065CysMet: 0.065 ± 0.079
0.589CysAsn: 0.589 ± 0.239
0.393CysPro: 0.393 ± 0.214
0.589CysGln: 0.589 ± 0.25
0.524CysArg: 0.524 ± 0.163
0.458CysSer: 0.458 ± 0.152
0.196CysThr: 0.196 ± 0.107
0.327CysVal: 0.327 ± 0.15
0.262CysTrp: 0.262 ± 0.105
0.327CysTyr: 0.327 ± 0.141
0.0CysXaa: 0.0 ± 0.0
Asp
6.742AspAla: 6.742 ± 0.808
1.047AspCys: 1.047 ± 0.301
4.582AspAsp: 4.582 ± 0.579
3.927AspGlu: 3.927 ± 0.626
2.553AspPhe: 2.553 ± 0.381
6.48AspGly: 6.48 ± 0.75
1.506AspHis: 1.506 ± 0.345
3.338AspIle: 3.338 ± 0.513
3.469AspLys: 3.469 ± 0.414
5.433AspLeu: 5.433 ± 0.667
1.571AspMet: 1.571 ± 0.313
2.16AspAsn: 2.16 ± 0.335
1.702AspPro: 1.702 ± 0.276
1.964AspGln: 1.964 ± 0.353
3.077AspArg: 3.077 ± 0.52
3.469AspSer: 3.469 ± 0.519
3.273AspThr: 3.273 ± 0.478
4.909AspVal: 4.909 ± 0.545
0.982AspTrp: 0.982 ± 0.232
2.095AspTyr: 2.095 ± 0.345
0.0AspXaa: 0.0 ± 0.0
Glu
5.564GluAla: 5.564 ± 0.67
0.589GluCys: 0.589 ± 0.165
3.142GluAsp: 3.142 ± 0.422
3.731GluGlu: 3.731 ± 0.54
2.684GluPhe: 2.684 ± 0.477
3.927GluGly: 3.927 ± 0.567
1.506GluHis: 1.506 ± 0.317
3.273GluIle: 3.273 ± 0.489
4.124GluLys: 4.124 ± 0.572
4.648GluLeu: 4.648 ± 0.632
2.16GluMet: 2.16 ± 0.376
2.291GluAsn: 2.291 ± 0.314
1.636GluPro: 1.636 ± 0.33
3.011GluGln: 3.011 ± 0.383
4.058GluArg: 4.058 ± 0.461
2.815GluSer: 2.815 ± 0.518
3.011GluThr: 3.011 ± 0.469
3.731GluVal: 3.731 ± 0.451
1.244GluTrp: 1.244 ± 0.32
2.291GluTyr: 2.291 ± 0.378
0.0GluXaa: 0.0 ± 0.0
Phe
3.404PheAla: 3.404 ± 0.419
0.589PheCys: 0.589 ± 0.209
3.404PheAsp: 3.404 ± 0.468
2.356PheGlu: 2.356 ± 0.457
1.113PhePhe: 1.113 ± 0.333
3.207PheGly: 3.207 ± 0.568
0.589PheHis: 0.589 ± 0.202
2.553PheIle: 2.553 ± 0.422
1.636PheLys: 1.636 ± 0.309
2.16PheLeu: 2.16 ± 0.427
1.178PheMet: 1.178 ± 0.278
2.16PheAsn: 2.16 ± 0.339
1.44PhePro: 1.44 ± 0.302
0.851PheGln: 0.851 ± 0.294
1.964PheArg: 1.964 ± 0.396
2.487PheSer: 2.487 ± 0.389
2.029PheThr: 2.029 ± 0.388
1.898PheVal: 1.898 ± 0.324
0.458PheTrp: 0.458 ± 0.2
1.113PheTyr: 1.113 ± 0.294
0.0PheXaa: 0.0 ± 0.0
Gly
6.808GlyAla: 6.808 ± 0.701
0.458GlyCys: 0.458 ± 0.208
4.844GlyAsp: 4.844 ± 0.554
4.517GlyGlu: 4.517 ± 0.609
2.684GlyPhe: 2.684 ± 0.567
6.48GlyGly: 6.48 ± 0.895
1.047GlyHis: 1.047 ± 0.225
4.844GlyIle: 4.844 ± 0.511
5.433GlyLys: 5.433 ± 0.698
5.106GlyLeu: 5.106 ± 0.661
2.684GlyMet: 2.684 ± 0.372
4.582GlyAsn: 4.582 ± 0.695
2.095GlyPro: 2.095 ± 0.377
3.469GlyGln: 3.469 ± 0.551
4.909GlyArg: 4.909 ± 0.476
5.368GlySer: 5.368 ± 0.623
3.731GlyThr: 3.731 ± 0.766
6.415GlyVal: 6.415 ± 0.599
0.982GlyTrp: 0.982 ± 0.212
3.338GlyTyr: 3.338 ± 0.501
0.0GlyXaa: 0.0 ± 0.0
His
1.767HisAla: 1.767 ± 0.425
0.262HisCys: 0.262 ± 0.16
1.506HisAsp: 1.506 ± 0.325
1.047HisGlu: 1.047 ± 0.294
0.655HisPhe: 0.655 ± 0.199
1.833HisGly: 1.833 ± 0.354
0.589HisHis: 0.589 ± 0.239
1.178HisIle: 1.178 ± 0.254
0.589HisLys: 0.589 ± 0.194
1.375HisLeu: 1.375 ± 0.323
0.196HisMet: 0.196 ± 0.11
1.309HisAsn: 1.309 ± 0.321
0.785HisPro: 0.785 ± 0.254
0.851HisGln: 0.851 ± 0.214
1.375HisArg: 1.375 ± 0.306
0.916HisSer: 0.916 ± 0.223
0.916HisThr: 0.916 ± 0.356
1.375HisVal: 1.375 ± 0.243
0.131HisTrp: 0.131 ± 0.082
1.244HisTyr: 1.244 ± 0.291
0.0HisXaa: 0.0 ± 0.0
Ile
6.546IleAla: 6.546 ± 0.673
0.524IleCys: 0.524 ± 0.21
4.124IleAsp: 4.124 ± 0.489
4.451IleGlu: 4.451 ± 0.48
1.767IlePhe: 1.767 ± 0.292
4.451IleGly: 4.451 ± 0.564
1.309IleHis: 1.309 ± 0.357
3.927IleIle: 3.927 ± 0.595
4.255IleLys: 4.255 ± 0.584
3.535IleLeu: 3.535 ± 0.5
1.44IleMet: 1.44 ± 0.313
2.684IleAsn: 2.684 ± 0.466
2.553IlePro: 2.553 ± 0.371
2.618IleGln: 2.618 ± 0.415
3.993IleArg: 3.993 ± 0.505
3.993IleSer: 3.993 ± 0.546
4.058IleThr: 4.058 ± 0.45
4.32IleVal: 4.32 ± 0.496
1.113IleTrp: 1.113 ± 0.267
1.833IleTyr: 1.833 ± 0.394
0.0IleXaa: 0.0 ± 0.0
Lys
6.153LysAla: 6.153 ± 0.539
0.589LysCys: 0.589 ± 0.207
3.6LysAsp: 3.6 ± 0.641
2.684LysGlu: 2.684 ± 0.422
2.226LysPhe: 2.226 ± 0.417
3.469LysGly: 3.469 ± 0.416
1.244LysHis: 1.244 ± 0.297
3.142LysIle: 3.142 ± 0.515
3.338LysLys: 3.338 ± 0.454
3.927LysLeu: 3.927 ± 0.511
2.618LysMet: 2.618 ± 0.42
2.422LysAsn: 2.422 ± 0.368
2.029LysPro: 2.029 ± 0.389
2.422LysGln: 2.422 ± 0.425
2.946LysArg: 2.946 ± 0.447
3.273LysSer: 3.273 ± 0.51
3.469LysThr: 3.469 ± 0.47
4.189LysVal: 4.189 ± 0.452
0.916LysTrp: 0.916 ± 0.248
1.506LysTyr: 1.506 ± 0.356
0.0LysXaa: 0.0 ± 0.0
Leu
6.939LeuAla: 6.939 ± 0.785
0.655LeuCys: 0.655 ± 0.19
4.582LeuAsp: 4.582 ± 0.558
3.666LeuGlu: 3.666 ± 0.582
2.291LeuPhe: 2.291 ± 0.405
4.189LeuGly: 4.189 ± 0.616
1.113LeuHis: 1.113 ± 0.275
4.909LeuIle: 4.909 ± 0.505
4.189LeuLys: 4.189 ± 0.551
4.386LeuLeu: 4.386 ± 0.479
2.095LeuMet: 2.095 ± 0.366
4.124LeuAsn: 4.124 ± 0.591
2.749LeuPro: 2.749 ± 0.442
2.422LeuGln: 2.422 ± 0.378
3.273LeuArg: 3.273 ± 0.45
6.415LeuSer: 6.415 ± 0.752
5.106LeuThr: 5.106 ± 0.474
5.237LeuVal: 5.237 ± 0.555
1.047LeuTrp: 1.047 ± 0.26
1.898LeuTyr: 1.898 ± 0.368
0.0LeuXaa: 0.0 ± 0.0
Met
3.338MetAla: 3.338 ± 0.48
0.131MetCys: 0.131 ± 0.096
1.375MetAsp: 1.375 ± 0.276
0.589MetGlu: 0.589 ± 0.218
0.655MetPhe: 0.655 ± 0.19
1.767MetGly: 1.767 ± 0.354
0.524MetHis: 0.524 ± 0.19
2.487MetIle: 2.487 ± 0.393
2.226MetLys: 2.226 ± 0.439
1.964MetLeu: 1.964 ± 0.321
0.982MetMet: 0.982 ± 0.258
1.047MetAsn: 1.047 ± 0.314
1.636MetPro: 1.636 ± 0.353
1.506MetGln: 1.506 ± 0.333
1.636MetArg: 1.636 ± 0.36
2.291MetSer: 2.291 ± 0.502
2.095MetThr: 2.095 ± 0.392
1.506MetVal: 1.506 ± 0.28
0.458MetTrp: 0.458 ± 0.157
0.72MetTyr: 0.72 ± 0.22
0.0MetXaa: 0.0 ± 0.0
Asn
4.451AsnAla: 4.451 ± 0.581
0.327AsnCys: 0.327 ± 0.163
2.618AsnAsp: 2.618 ± 0.528
2.618AsnGlu: 2.618 ± 0.446
2.095AsnPhe: 2.095 ± 0.309
3.731AsnGly: 3.731 ± 0.499
1.375AsnHis: 1.375 ± 0.395
3.142AsnIle: 3.142 ± 0.456
2.16AsnLys: 2.16 ± 0.449
3.731AsnLeu: 3.731 ± 0.436
1.113AsnMet: 1.113 ± 0.265
2.815AsnAsn: 2.815 ± 0.382
2.356AsnPro: 2.356 ± 0.311
1.898AsnGln: 1.898 ± 0.386
1.964AsnArg: 1.964 ± 0.388
2.815AsnSer: 2.815 ± 0.42
2.88AsnThr: 2.88 ± 0.493
3.207AsnVal: 3.207 ± 0.39
0.327AsnTrp: 0.327 ± 0.125
1.636AsnTyr: 1.636 ± 0.288
0.0AsnXaa: 0.0 ± 0.0
Pro
3.666ProAla: 3.666 ± 0.5
0.327ProCys: 0.327 ± 0.148
3.207ProAsp: 3.207 ± 0.423
2.356ProGlu: 2.356 ± 0.389
1.506ProPhe: 1.506 ± 0.311
3.207ProGly: 3.207 ± 0.442
0.327ProHis: 0.327 ± 0.142
2.684ProIle: 2.684 ± 0.454
1.506ProLys: 1.506 ± 0.335
1.898ProLeu: 1.898 ± 0.305
0.655ProMet: 0.655 ± 0.199
1.898ProAsn: 1.898 ± 0.364
1.44ProPro: 1.44 ± 0.296
1.375ProGln: 1.375 ± 0.345
1.636ProArg: 1.636 ± 0.301
1.767ProSer: 1.767 ± 0.433
1.636ProThr: 1.636 ± 0.258
3.6ProVal: 3.6 ± 0.415
0.458ProTrp: 0.458 ± 0.189
0.982ProTyr: 0.982 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
3.404GlnAla: 3.404 ± 0.498
0.327GlnCys: 0.327 ± 0.135
1.702GlnAsp: 1.702 ± 0.353
1.964GlnGlu: 1.964 ± 0.382
1.44GlnPhe: 1.44 ± 0.237
2.88GlnGly: 2.88 ± 0.384
0.982GlnHis: 0.982 ± 0.28
3.077GlnIle: 3.077 ± 0.428
1.833GlnLys: 1.833 ± 0.319
3.797GlnLeu: 3.797 ± 0.367
1.244GlnMet: 1.244 ± 0.256
1.636GlnAsn: 1.636 ± 0.315
1.244GlnPro: 1.244 ± 0.311
2.291GlnGln: 2.291 ± 0.456
3.469GlnArg: 3.469 ± 0.534
1.833GlnSer: 1.833 ± 0.357
2.684GlnThr: 2.684 ± 0.414
2.553GlnVal: 2.553 ± 0.423
0.327GlnTrp: 0.327 ± 0.138
1.375GlnTyr: 1.375 ± 0.392
0.0GlnXaa: 0.0 ± 0.0
Arg
4.909ArgAla: 4.909 ± 0.647
1.113ArgCys: 1.113 ± 0.318
2.815ArgAsp: 2.815 ± 0.367
3.142ArgGlu: 3.142 ± 0.573
2.291ArgPhe: 2.291 ± 0.457
3.927ArgGly: 3.927 ± 0.495
1.047ArgHis: 1.047 ± 0.302
3.862ArgIle: 3.862 ± 0.468
3.993ArgLys: 3.993 ± 0.42
4.189ArgLeu: 4.189 ± 0.6
1.636ArgMet: 1.636 ± 0.302
2.16ArgAsn: 2.16 ± 0.378
1.964ArgPro: 1.964 ± 0.352
2.684ArgGln: 2.684 ± 0.411
3.142ArgArg: 3.142 ± 0.445
3.338ArgSer: 3.338 ± 0.4
2.422ArgThr: 2.422 ± 0.371
4.648ArgVal: 4.648 ± 0.526
0.524ArgTrp: 0.524 ± 0.208
2.226ArgTyr: 2.226 ± 0.405
0.0ArgXaa: 0.0 ± 0.0
Ser
4.975SerAla: 4.975 ± 0.62
0.262SerCys: 0.262 ± 0.141
4.713SerAsp: 4.713 ± 0.675
4.058SerGlu: 4.058 ± 0.521
2.749SerPhe: 2.749 ± 0.388
5.957SerGly: 5.957 ± 0.581
1.309SerHis: 1.309 ± 0.329
3.273SerIle: 3.273 ± 0.437
2.749SerLys: 2.749 ± 0.444
5.368SerLeu: 5.368 ± 0.67
1.571SerMet: 1.571 ± 0.305
3.011SerAsn: 3.011 ± 0.481
2.16SerPro: 2.16 ± 0.413
2.618SerGln: 2.618 ± 0.358
3.731SerArg: 3.731 ± 0.522
2.553SerSer: 2.553 ± 0.408
3.273SerThr: 3.273 ± 0.48
4.778SerVal: 4.778 ± 0.57
0.72SerTrp: 0.72 ± 0.239
1.767SerTyr: 1.767 ± 0.341
0.0SerXaa: 0.0 ± 0.0
Thr
5.695ThrAla: 5.695 ± 0.66
0.524ThrCys: 0.524 ± 0.183
4.255ThrAsp: 4.255 ± 0.513
3.011ThrGlu: 3.011 ± 0.446
1.702ThrPhe: 1.702 ± 0.292
6.153ThrGly: 6.153 ± 0.699
1.178ThrHis: 1.178 ± 0.289
3.731ThrIle: 3.731 ± 0.559
2.946ThrLys: 2.946 ± 0.526
4.255ThrLeu: 4.255 ± 0.499
1.636ThrMet: 1.636 ± 0.311
1.898ThrAsn: 1.898 ± 0.301
2.749ThrPro: 2.749 ± 0.435
1.898ThrGln: 1.898 ± 0.378
2.946ThrArg: 2.946 ± 0.47
2.946ThrSer: 2.946 ± 0.355
3.404ThrThr: 3.404 ± 0.504
3.927ThrVal: 3.927 ± 0.542
0.524ThrTrp: 0.524 ± 0.196
1.898ThrTyr: 1.898 ± 0.368
0.0ThrXaa: 0.0 ± 0.0
Val
5.76ValAla: 5.76 ± 0.671
0.524ValCys: 0.524 ± 0.203
4.844ValAsp: 4.844 ± 0.428
3.927ValGlu: 3.927 ± 0.447
2.88ValPhe: 2.88 ± 0.439
4.648ValGly: 4.648 ± 0.505
0.982ValHis: 0.982 ± 0.263
5.106ValIle: 5.106 ± 0.601
4.058ValLys: 4.058 ± 0.508
3.731ValLeu: 3.731 ± 0.441
1.767ValMet: 1.767 ± 0.37
4.909ValAsn: 4.909 ± 0.572
2.88ValPro: 2.88 ± 0.439
1.767ValGln: 1.767 ± 0.361
3.666ValArg: 3.666 ± 0.507
5.498ValSer: 5.498 ± 0.609
5.302ValThr: 5.302 ± 0.592
4.32ValVal: 4.32 ± 0.526
0.982ValTrp: 0.982 ± 0.279
2.815ValTyr: 2.815 ± 0.403
0.0ValXaa: 0.0 ± 0.0
Trp
1.244TrpAla: 1.244 ± 0.385
0.065TrpCys: 0.065 ± 0.072
1.178TrpAsp: 1.178 ± 0.223
0.851TrpGlu: 0.851 ± 0.237
0.262TrpPhe: 0.262 ± 0.132
0.589TrpGly: 0.589 ± 0.172
0.458TrpHis: 0.458 ± 0.153
0.589TrpIle: 0.589 ± 0.206
0.982TrpLys: 0.982 ± 0.225
1.178TrpLeu: 1.178 ± 0.311
0.196TrpMet: 0.196 ± 0.105
0.524TrpAsn: 0.524 ± 0.201
0.393TrpPro: 0.393 ± 0.181
0.785TrpGln: 0.785 ± 0.23
1.375TrpArg: 1.375 ± 0.33
0.655TrpSer: 0.655 ± 0.252
0.72TrpThr: 0.72 ± 0.211
1.047TrpVal: 1.047 ± 0.31
0.065TrpTrp: 0.065 ± 0.057
0.196TrpTyr: 0.196 ± 0.11
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.095TyrAla: 2.095 ± 0.441
0.262TyrCys: 0.262 ± 0.127
1.767TyrAsp: 1.767 ± 0.355
2.291TyrGlu: 2.291 ± 0.366
1.506TyrPhe: 1.506 ± 0.309
3.338TyrGly: 3.338 ± 0.535
0.851TyrHis: 0.851 ± 0.23
1.113TyrIle: 1.113 ± 0.233
1.244TyrLys: 1.244 ± 0.31
2.356TyrLeu: 2.356 ± 0.369
1.047TyrMet: 1.047 ± 0.291
1.375TyrAsn: 1.375 ± 0.274
1.571TyrPro: 1.571 ± 0.392
1.702TyrGln: 1.702 ± 0.341
2.095TyrArg: 2.095 ± 0.389
2.618TyrSer: 2.618 ± 0.438
2.553TyrThr: 2.553 ± 0.379
2.16TyrVal: 2.16 ± 0.328
0.458TyrTrp: 0.458 ± 0.197
1.047TyrTyr: 1.047 ± 0.341
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (15278 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski