Mycobacterium phage Bxz2 (Mycobacteriophage Bxz2)

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q857E6|Q857E6_BPMB2 Uncharacterized protein OS=Mycobacterium phage Bxz2 OX=205870 GN=76 PE=4 SV=1
MM1 pKa = 7.89EE2 pKa = 5.9IGNVDD7 pKa = 3.53QVKK10 pKa = 10.36SALEE14 pKa = 4.06SFRR17 pKa = 11.84SMLQLKK23 pKa = 10.17HH24 pKa = 6.86DD25 pKa = 5.14FIEE28 pKa = 4.54EE29 pKa = 4.06NQDD32 pKa = 3.39PDD34 pKa = 4.63DD35 pKa = 4.84GEE37 pKa = 4.5PEE39 pKa = 4.21CGYY42 pKa = 10.72SRR44 pKa = 11.84WDD46 pKa = 3.67EE47 pKa = 4.76AITDD51 pKa = 3.85YY52 pKa = 11.16NYY54 pKa = 10.32EE55 pKa = 3.71LAYY58 pKa = 10.82YY59 pKa = 10.44GEE61 pKa = 4.21QLADD65 pKa = 3.58AVAEE69 pKa = 3.83ALGIKK74 pKa = 10.29EE75 pKa = 3.9EE76 pKa = 4.34DD77 pKa = 3.27

Molecular weight: 8.81 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q857F3|Q857F3_BPMB2 Uncharacterized protein OS=Mycobacterium phage Bxz2 OX=205870 GN=69 PE=4 SV=1
MM1 pKa = 6.74TVKK4 pKa = 10.63VNDD7 pKa = 3.85RR8 pKa = 11.84KK9 pKa = 10.65LEE11 pKa = 4.43PGTEE15 pKa = 3.96VSIKK19 pKa = 10.17GEE21 pKa = 3.73RR22 pKa = 11.84GRR24 pKa = 11.84FRR26 pKa = 11.84FVKK29 pKa = 9.64STTTSQGKK37 pKa = 7.26TVLDD41 pKa = 4.81FIGGPAGHH49 pKa = 5.74EE50 pKa = 4.16QWRR53 pKa = 11.84SFYY56 pKa = 9.51PEE58 pKa = 3.66RR59 pKa = 11.84VEE61 pKa = 4.27TVHH64 pKa = 7.27RR65 pKa = 11.84IARR68 pKa = 11.84TRR70 pKa = 11.84ANAKK74 pKa = 9.79KK75 pKa = 10.47

Molecular weight: 8.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

15408

20

1008

179.2

19.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.014 ± 0.392

0.811 ± 0.123

6.36 ± 0.241

6.737 ± 0.354

3.466 ± 0.191

8.638 ± 0.545

2.077 ± 0.181

4.679 ± 0.178

4.277 ± 0.228

8.165 ± 0.312

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.186

3.368 ± 0.253

5.517 ± 0.378

3.894 ± 0.276

6.763 ± 0.33

5.134 ± 0.211

5.925 ± 0.238

6.86 ± 0.22

1.992 ± 0.155

2.895 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski