Arthrobacter crystallopoietes BAB-32

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Micrococcaceae; Arthrobacter; Arthrobacter crystallopoietes

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N1V3T6|N1V3T6_9MICC Uncharacterized protein (Fragment) OS=Arthrobacter crystallopoietes BAB-32 OX=1246476 GN=D477_001264 PE=4 SV=1
MM1 pKa = 8.0PEE3 pKa = 3.95TPRR6 pKa = 11.84GQLVCKK12 pKa = 10.67ASDD15 pKa = 3.71VQVKK19 pKa = 8.96QALRR23 pKa = 11.84ILIDD27 pKa = 4.44DD28 pKa = 3.99YY29 pKa = 11.38PVAVVRR35 pKa = 11.84DD36 pKa = 3.82SDD38 pKa = 4.45GEE40 pKa = 4.12LHH42 pKa = 7.15ALGDD46 pKa = 4.11TCSHH50 pKa = 6.6EE51 pKa = 5.22DD52 pKa = 3.56YY53 pKa = 10.86SLSEE57 pKa = 4.56GDD59 pKa = 5.25VEE61 pKa = 5.6DD62 pKa = 4.63CTIEE66 pKa = 4.11CWGHH70 pKa = 6.36GSQFDD75 pKa = 4.08LRR77 pKa = 11.84SGAPLSLPAYY87 pKa = 10.09DD88 pKa = 4.21PVPVFALTVDD98 pKa = 3.63GDD100 pKa = 4.01DD101 pKa = 3.92VYY103 pKa = 12.12VDD105 pKa = 3.4VTTVTNGAPAPTSNN119 pKa = 3.98

Molecular weight: 12.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N1V8J9|N1V8J9_9MICC Phenylacetate-CoA oxygenase subunit PaaK OS=Arthrobacter crystallopoietes BAB-32 OX=1246476 GN=D477_009228 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4118

0

4118

1230685

26

2025

298.9

32.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.164 ± 0.06

0.634 ± 0.011

5.662 ± 0.034

6.204 ± 0.034

3.155 ± 0.025

9.088 ± 0.039

2.069 ± 0.019

4.271 ± 0.032

2.631 ± 0.025

10.342 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.019 ± 0.015

2.384 ± 0.021

5.471 ± 0.028

3.316 ± 0.02

6.775 ± 0.037

5.552 ± 0.023

5.562 ± 0.026

8.159 ± 0.038

1.431 ± 0.017

2.111 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski