Cellulophaga phage phiST

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cbastvirus; Cellulophaga virus ST

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9ZZ12|R9ZZ12_9CAUD DNA polymerase III alpha subunit OS=Cellulophaga phage phiST OX=756282 GN=PhiST_gp044 PE=4 SV=1
MM1 pKa = 6.92TTFTTLINEE10 pKa = 4.09NFLLNTNTIYY20 pKa = 10.93GQVIAKK26 pKa = 9.87AVLGSILIVSFVDD39 pKa = 3.19NNEE42 pKa = 3.74VVTIEE47 pKa = 4.87FEE49 pKa = 4.1EE50 pKa = 4.8CMGNLVQSS58 pKa = 4.1

Molecular weight: 6.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4SNA7|M4SNA7_9CAUD Uncharacterized protein OS=Cellulophaga phage phiST OX=756282 GN=CGPG_00067 PE=4 SV=1
MM1 pKa = 7.02KK2 pKa = 10.48QEE4 pKa = 4.69LFQTNQIFIFILISWFIVGASCYY27 pKa = 9.31ALSWIEE33 pKa = 3.31IHH35 pKa = 7.1RR36 pKa = 11.84RR37 pKa = 11.84TNKK40 pKa = 10.42PIDD43 pKa = 4.03PKK45 pKa = 11.16QLFQQSLFYY54 pKa = 10.86SGLGFFMVIITIIFFYY70 pKa = 9.7QNRR73 pKa = 11.84RR74 pKa = 3.36

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

23280

35

1248

213.6

24.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.082 ± 0.275

0.997 ± 0.108

6.624 ± 0.173

7.302 ± 0.302

4.682 ± 0.157

5.911 ± 0.384

1.499 ± 0.15

8.793 ± 0.263

8.836 ± 0.363

8.767 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.149

5.971 ± 0.229

2.564 ± 0.12

3.986 ± 0.169

3.763 ± 0.151

7.337 ± 0.198

5.322 ± 0.27

5.46 ± 0.176

1.121 ± 0.072

3.686 ± 0.227
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski