Amino acid dipepetide frequency for Cellulophaga phage phiST

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.706AlaAla: 2.706 ± 0.525
0.301AlaCys: 0.301 ± 0.11
3.007AlaAsp: 3.007 ± 0.335
4.124AlaGlu: 4.124 ± 0.475
2.105AlaPhe: 2.105 ± 0.373
2.406AlaGly: 2.406 ± 0.399
0.687AlaHis: 0.687 ± 0.157
4.94AlaIle: 4.94 ± 0.544
5.155AlaLys: 5.155 ± 0.466
4.468AlaLeu: 4.468 ± 0.53
1.546AlaMet: 1.546 ± 0.261
3.351AlaAsn: 3.351 ± 0.4
1.203AlaPro: 1.203 ± 0.242
1.933AlaGln: 1.933 ± 0.348
2.363AlaArg: 2.363 ± 0.429
3.351AlaSer: 3.351 ± 0.394
2.148AlaThr: 2.148 ± 0.303
2.749AlaVal: 2.749 ± 0.304
0.515AlaTrp: 0.515 ± 0.174
1.761AlaTyr: 1.761 ± 0.255
0.0AlaXaa: 0.0 ± 0.0
Cys
0.687CysAla: 0.687 ± 0.197
0.086CysCys: 0.086 ± 0.055
0.601CysAsp: 0.601 ± 0.185
0.859CysGlu: 0.859 ± 0.207
0.387CysPhe: 0.387 ± 0.148
0.601CysGly: 0.601 ± 0.149
0.129CysHis: 0.129 ± 0.077
0.601CysIle: 0.601 ± 0.158
0.558CysLys: 0.558 ± 0.166
0.73CysLeu: 0.73 ± 0.194
0.129CysMet: 0.129 ± 0.072
0.644CysAsn: 0.644 ± 0.177
0.344CysPro: 0.344 ± 0.132
0.473CysGln: 0.473 ± 0.148
0.43CysArg: 0.43 ± 0.139
1.246CysSer: 1.246 ± 0.28
0.43CysThr: 0.43 ± 0.135
0.558CysVal: 0.558 ± 0.139
0.129CysTrp: 0.129 ± 0.084
0.344CysTyr: 0.344 ± 0.124
0.0CysXaa: 0.0 ± 0.0
Asp
3.222AspAla: 3.222 ± 0.395
1.074AspCys: 1.074 ± 0.21
4.081AspAsp: 4.081 ± 0.399
4.081AspGlu: 4.081 ± 0.435
3.522AspPhe: 3.522 ± 0.38
3.995AspGly: 3.995 ± 0.416
1.031AspHis: 1.031 ± 0.211
5.713AspIle: 5.713 ± 0.519
4.639AspLys: 4.639 ± 0.402
7.346AspLeu: 7.346 ± 0.566
1.418AspMet: 1.418 ± 0.273
3.179AspAsn: 3.179 ± 0.328
2.277AspPro: 2.277 ± 0.328
3.007AspGln: 3.007 ± 0.419
2.663AspArg: 2.663 ± 0.319
4.596AspSer: 4.596 ± 0.402
2.148AspThr: 2.148 ± 0.378
3.437AspVal: 3.437 ± 0.434
1.418AspTrp: 1.418 ± 0.244
3.394AspTyr: 3.394 ± 0.323
0.0AspXaa: 0.0 ± 0.0
Glu
3.78GluAla: 3.78 ± 0.475
0.73GluCys: 0.73 ± 0.215
5.026GluAsp: 5.026 ± 0.491
5.456GluGlu: 5.456 ± 0.694
3.093GluPhe: 3.093 ± 0.372
4.468GluGly: 4.468 ± 0.467
0.601GluHis: 0.601 ± 0.14
7.174GluIle: 7.174 ± 0.643
7.217GluLys: 7.217 ± 0.72
7.689GluLeu: 7.689 ± 0.644
1.89GluMet: 1.89 ± 0.292
4.468GluAsn: 4.468 ± 0.5
1.031GluPro: 1.031 ± 0.18
2.835GluGln: 2.835 ± 0.375
2.492GluArg: 2.492 ± 0.392
5.026GluSer: 5.026 ± 0.457
2.921GluThr: 2.921 ± 0.345
3.694GluVal: 3.694 ± 0.341
0.902GluTrp: 0.902 ± 0.213
2.534GluTyr: 2.534 ± 0.379
0.0GluXaa: 0.0 ± 0.0
Phe
1.933PheAla: 1.933 ± 0.319
0.515PheCys: 0.515 ± 0.154
3.995PheAsp: 3.995 ± 0.395
4.425PheGlu: 4.425 ± 0.431
1.632PhePhe: 1.632 ± 0.247
3.222PheGly: 3.222 ± 0.331
0.773PheHis: 0.773 ± 0.168
3.694PheIle: 3.694 ± 0.356
3.222PheLys: 3.222 ± 0.364
4.468PheLeu: 4.468 ± 0.419
1.074PheMet: 1.074 ± 0.241
2.835PheAsn: 2.835 ± 0.421
1.504PhePro: 1.504 ± 0.27
1.718PheGln: 1.718 ± 0.246
1.761PheArg: 1.761 ± 0.216
3.565PheSer: 3.565 ± 0.344
2.449PheThr: 2.449 ± 0.311
2.492PheVal: 2.492 ± 0.439
0.086PheTrp: 0.086 ± 0.064
1.461PheTyr: 1.461 ± 0.242
0.0PheXaa: 0.0 ± 0.0
Gly
2.32GlyAla: 2.32 ± 0.469
0.644GlyCys: 0.644 ± 0.188
4.553GlyAsp: 4.553 ± 0.577
4.296GlyGlu: 4.296 ± 0.582
2.964GlyPhe: 2.964 ± 0.329
4.21GlyGly: 4.21 ± 0.494
0.773GlyHis: 0.773 ± 0.301
4.553GlyIle: 4.553 ± 0.485
5.413GlyLys: 5.413 ± 0.439
5.627GlyLeu: 5.627 ± 0.474
1.332GlyMet: 1.332 ± 0.229
2.921GlyAsn: 2.921 ± 0.358
0.816GlyPro: 0.816 ± 0.254
1.546GlyGln: 1.546 ± 0.275
2.406GlyArg: 2.406 ± 0.334
4.468GlySer: 4.468 ± 0.491
3.437GlyThr: 3.437 ± 0.582
2.878GlyVal: 2.878 ± 0.4
1.031GlyTrp: 1.031 ± 0.181
2.921GlyTyr: 2.921 ± 0.355
0.0GlyXaa: 0.0 ± 0.0
His
0.473HisAla: 0.473 ± 0.131
0.172HisCys: 0.172 ± 0.083
0.816HisAsp: 0.816 ± 0.162
0.73HisGlu: 0.73 ± 0.168
0.902HisPhe: 0.902 ± 0.217
1.074HisGly: 1.074 ± 0.24
0.43HisHis: 0.43 ± 0.154
1.117HisIle: 1.117 ± 0.216
1.289HisLys: 1.289 ± 0.216
1.461HisLeu: 1.461 ± 0.293
0.258HisMet: 0.258 ± 0.1
0.859HisAsn: 0.859 ± 0.2
0.687HisPro: 0.687 ± 0.155
0.773HisGln: 0.773 ± 0.191
0.644HisArg: 0.644 ± 0.168
1.203HisSer: 1.203 ± 0.233
0.515HisThr: 0.515 ± 0.149
0.773HisVal: 0.773 ± 0.2
0.43HisTrp: 0.43 ± 0.167
0.387HisTyr: 0.387 ± 0.141
0.0HisXaa: 0.0 ± 0.0
Ile
5.327IleAla: 5.327 ± 0.604
0.687IleCys: 0.687 ± 0.166
5.971IleAsp: 5.971 ± 0.548
8.119IleGlu: 8.119 ± 0.802
4.167IlePhe: 4.167 ± 0.529
4.253IleGly: 4.253 ± 0.524
1.632IleHis: 1.632 ± 0.3
6.787IleIle: 6.787 ± 0.714
8.806IleLys: 8.806 ± 0.665
6.572IleLeu: 6.572 ± 0.494
1.804IleMet: 1.804 ± 0.321
6.315IleAsn: 6.315 ± 0.621
2.835IlePro: 2.835 ± 0.381
3.007IleGln: 3.007 ± 0.319
2.792IleArg: 2.792 ± 0.341
6.229IleSer: 6.229 ± 0.573
4.983IleThr: 4.983 ± 0.392
4.425IleVal: 4.425 ± 0.45
0.644IleTrp: 0.644 ± 0.163
2.577IleTyr: 2.577 ± 0.461
0.0IleXaa: 0.0 ± 0.0
Lys
5.284LysAla: 5.284 ± 0.433
0.687LysCys: 0.687 ± 0.21
6.014LysAsp: 6.014 ± 0.641
5.885LysGlu: 5.885 ± 0.56
3.909LysPhe: 3.909 ± 0.446
4.596LysGly: 4.596 ± 0.734
1.246LysHis: 1.246 ± 0.189
6.358LysIle: 6.358 ± 0.532
7.646LysLys: 7.646 ± 0.692
7.346LysLeu: 7.346 ± 0.581
2.706LysMet: 2.706 ± 0.375
5.155LysAsn: 5.155 ± 0.475
2.191LysPro: 2.191 ± 0.294
4.639LysGln: 4.639 ± 0.541
2.921LysArg: 2.921 ± 0.472
4.811LysSer: 4.811 ± 0.497
5.971LysThr: 5.971 ± 0.605
6.401LysVal: 6.401 ± 0.606
0.816LysTrp: 0.816 ± 0.212
3.737LysTyr: 3.737 ± 0.423
0.0LysXaa: 0.0 ± 0.0
Leu
3.737LeuAla: 3.737 ± 0.593
1.117LeuCys: 1.117 ± 0.274
6.83LeuAsp: 6.83 ± 0.543
6.744LeuGlu: 6.744 ± 0.801
3.608LeuPhe: 3.608 ± 0.427
5.112LeuGly: 5.112 ± 0.39
0.73LeuHis: 0.73 ± 0.182
8.334LeuIle: 8.334 ± 0.667
7.603LeuLys: 7.603 ± 0.675
7.346LeuLeu: 7.346 ± 0.631
2.019LeuMet: 2.019 ± 0.318
4.854LeuAsn: 4.854 ± 0.437
2.406LeuPro: 2.406 ± 0.34
3.351LeuGln: 3.351 ± 0.425
3.48LeuArg: 3.48 ± 0.367
7.732LeuSer: 7.732 ± 0.42
4.725LeuThr: 4.725 ± 0.479
4.811LeuVal: 4.811 ± 0.389
0.73LeuTrp: 0.73 ± 0.186
2.406LeuTyr: 2.406 ± 0.301
0.0LeuXaa: 0.0 ± 0.0
Met
1.332MetAla: 1.332 ± 0.207
0.215MetCys: 0.215 ± 0.106
0.687MetAsp: 0.687 ± 0.182
1.332MetGlu: 1.332 ± 0.209
0.859MetPhe: 0.859 ± 0.186
0.816MetGly: 0.816 ± 0.235
0.43MetHis: 0.43 ± 0.137
2.148MetIle: 2.148 ± 0.297
3.394MetLys: 3.394 ± 0.378
1.332MetLeu: 1.332 ± 0.228
0.644MetMet: 0.644 ± 0.167
1.761MetAsn: 1.761 ± 0.283
0.387MetPro: 0.387 ± 0.119
1.031MetGln: 1.031 ± 0.209
1.203MetArg: 1.203 ± 0.204
1.976MetSer: 1.976 ± 0.281
1.289MetThr: 1.289 ± 0.241
1.461MetVal: 1.461 ± 0.309
0.215MetTrp: 0.215 ± 0.107
0.473MetTyr: 0.473 ± 0.158
0.0MetXaa: 0.0 ± 0.0
Asn
3.05AsnAla: 3.05 ± 0.419
0.601AsnCys: 0.601 ± 0.185
2.792AsnAsp: 2.792 ± 0.287
4.038AsnGlu: 4.038 ± 0.38
2.964AsnPhe: 2.964 ± 0.43
3.737AsnGly: 3.737 ± 0.598
0.945AsnHis: 0.945 ± 0.214
5.241AsnIle: 5.241 ± 0.552
4.682AsnLys: 4.682 ± 0.533
5.756AsnLeu: 5.756 ± 0.671
1.203AsnMet: 1.203 ± 0.211
3.995AsnAsn: 3.995 ± 0.588
2.148AsnPro: 2.148 ± 0.284
2.749AsnGln: 2.749 ± 0.386
2.492AsnArg: 2.492 ± 0.293
4.038AsnSer: 4.038 ± 0.432
2.749AsnThr: 2.749 ± 0.377
3.222AsnVal: 3.222 ± 0.376
0.558AsnTrp: 0.558 ± 0.15
2.749AsnTyr: 2.749 ± 0.343
0.0AsnXaa: 0.0 ± 0.0
Pro
1.117ProAla: 1.117 ± 0.238
0.129ProCys: 0.129 ± 0.075
1.504ProAsp: 1.504 ± 0.309
1.546ProGlu: 1.546 ± 0.256
1.332ProPhe: 1.332 ± 0.247
0.301ProGly: 0.301 ± 0.12
0.558ProHis: 0.558 ± 0.172
2.191ProIle: 2.191 ± 0.318
2.406ProLys: 2.406 ± 0.386
1.976ProLeu: 1.976 ± 0.342
0.601ProMet: 0.601 ± 0.13
2.363ProAsn: 2.363 ± 0.322
0.43ProPro: 0.43 ± 0.145
1.203ProGln: 1.203 ± 0.211
0.687ProArg: 0.687 ± 0.191
1.933ProSer: 1.933 ± 0.286
2.792ProThr: 2.792 ± 0.304
1.074ProVal: 1.074 ± 0.171
0.172ProTrp: 0.172 ± 0.079
1.332ProTyr: 1.332 ± 0.276
0.0ProXaa: 0.0 ± 0.0
Gln
2.449GlnAla: 2.449 ± 0.321
0.301GlnCys: 0.301 ± 0.12
2.577GlnAsp: 2.577 ± 0.349
2.663GlnGlu: 2.663 ± 0.301
1.933GlnPhe: 1.933 ± 0.281
2.62GlnGly: 2.62 ± 0.329
0.258GlnHis: 0.258 ± 0.096
3.651GlnIle: 3.651 ± 0.375
3.651GlnLys: 3.651 ± 0.41
3.78GlnLeu: 3.78 ± 0.446
1.16GlnMet: 1.16 ± 0.248
2.019GlnAsn: 2.019 ± 0.292
0.644GlnPro: 0.644 ± 0.167
1.675GlnGln: 1.675 ± 0.254
1.761GlnArg: 1.761 ± 0.241
2.792GlnSer: 2.792 ± 0.347
2.148GlnThr: 2.148 ± 0.332
2.019GlnVal: 2.019 ± 0.302
0.558GlnTrp: 0.558 ± 0.146
1.203GlnTyr: 1.203 ± 0.213
0.0GlnXaa: 0.0 ± 0.0
Arg
1.761ArgAla: 1.761 ± 0.299
0.258ArgCys: 0.258 ± 0.111
2.878ArgAsp: 2.878 ± 0.429
2.663ArgGlu: 2.663 ± 0.359
2.062ArgPhe: 2.062 ± 0.26
2.32ArgGly: 2.32 ± 0.395
0.558ArgHis: 0.558 ± 0.123
2.878ArgIle: 2.878 ± 0.332
3.093ArgLys: 3.093 ± 0.39
2.964ArgLeu: 2.964 ± 0.354
1.16ArgMet: 1.16 ± 0.313
1.804ArgAsn: 1.804 ± 0.293
1.375ArgPro: 1.375 ± 0.196
1.504ArgGln: 1.504 ± 0.253
1.461ArgArg: 1.461 ± 0.332
2.835ArgSer: 2.835 ± 0.428
1.675ArgThr: 1.675 ± 0.264
1.976ArgVal: 1.976 ± 0.265
0.558ArgTrp: 0.558 ± 0.145
1.847ArgTyr: 1.847 ± 0.27
0.0ArgXaa: 0.0 ± 0.0
Ser
3.136SerAla: 3.136 ± 0.312
1.074SerCys: 1.074 ± 0.229
4.94SerAsp: 4.94 ± 0.378
5.155SerGlu: 5.155 ± 0.463
3.394SerPhe: 3.394 ± 0.386
4.811SerGly: 4.811 ± 0.36
1.332SerHis: 1.332 ± 0.248
7.518SerIle: 7.518 ± 0.697
7.088SerLys: 7.088 ± 0.518
5.541SerLeu: 5.541 ± 0.534
1.203SerMet: 1.203 ± 0.219
5.026SerAsn: 5.026 ± 0.533
1.761SerPro: 1.761 ± 0.289
2.663SerGln: 2.663 ± 0.353
2.492SerArg: 2.492 ± 0.372
5.284SerSer: 5.284 ± 0.715
4.382SerThr: 4.382 ± 0.532
3.651SerVal: 3.651 ± 0.396
0.73SerTrp: 0.73 ± 0.195
2.191SerTyr: 2.191 ± 0.286
0.0SerXaa: 0.0 ± 0.0
Thr
2.663ThrAla: 2.663 ± 0.324
0.344ThrCys: 0.344 ± 0.102
2.32ThrAsp: 2.32 ± 0.304
3.308ThrGlu: 3.308 ± 0.311
3.007ThrPhe: 3.007 ± 0.363
3.651ThrGly: 3.651 ± 0.565
0.859ThrHis: 0.859 ± 0.231
6.572ThrIle: 6.572 ± 0.558
4.253ThrLys: 4.253 ± 0.389
4.811ThrLeu: 4.811 ± 0.541
0.902ThrMet: 0.902 ± 0.211
3.093ThrAsn: 3.093 ± 0.421
1.246ThrPro: 1.246 ± 0.286
2.191ThrGln: 2.191 ± 0.306
1.804ThrArg: 1.804 ± 0.292
3.737ThrSer: 3.737 ± 0.492
3.351ThrThr: 3.351 ± 0.322
2.62ThrVal: 2.62 ± 0.354
0.387ThrTrp: 0.387 ± 0.138
2.105ThrTyr: 2.105 ± 0.276
0.0ThrXaa: 0.0 ± 0.0
Val
3.308ValAla: 3.308 ± 0.428
0.515ValCys: 0.515 ± 0.168
4.425ValAsp: 4.425 ± 0.458
4.425ValGlu: 4.425 ± 0.478
2.792ValPhe: 2.792 ± 0.314
3.823ValGly: 3.823 ± 0.467
0.773ValHis: 0.773 ± 0.21
4.339ValIle: 4.339 ± 0.409
4.382ValLys: 4.382 ± 0.371
4.253ValLeu: 4.253 ± 0.45
1.289ValMet: 1.289 ± 0.233
3.05ValAsn: 3.05 ± 0.394
1.203ValPro: 1.203 ± 0.197
1.804ValGln: 1.804 ± 0.308
1.804ValArg: 1.804 ± 0.237
4.253ValSer: 4.253 ± 0.417
2.663ValThr: 2.663 ± 0.312
3.179ValVal: 3.179 ± 0.448
0.773ValTrp: 0.773 ± 0.213
1.546ValTyr: 1.546 ± 0.301
0.0ValXaa: 0.0 ± 0.0
Trp
0.301TrpAla: 0.301 ± 0.101
0.129TrpCys: 0.129 ± 0.082
0.515TrpAsp: 0.515 ± 0.14
1.074TrpGlu: 1.074 ± 0.21
0.43TrpPhe: 0.43 ± 0.122
0.687TrpGly: 0.687 ± 0.179
0.258TrpHis: 0.258 ± 0.126
1.332TrpIle: 1.332 ± 0.266
1.203TrpLys: 1.203 ± 0.234
0.73TrpLeu: 0.73 ± 0.205
0.129TrpMet: 0.129 ± 0.084
0.43TrpAsn: 0.43 ± 0.12
0.043TrpPro: 0.043 ± 0.038
0.215TrpGln: 0.215 ± 0.091
0.43TrpArg: 0.43 ± 0.141
1.117TrpSer: 1.117 ± 0.212
0.73TrpThr: 0.73 ± 0.157
0.859TrpVal: 0.859 ± 0.168
0.172TrpTrp: 0.172 ± 0.113
0.43TrpTyr: 0.43 ± 0.128
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.234TyrAla: 2.234 ± 0.298
0.387TyrCys: 0.387 ± 0.131
2.706TyrAsp: 2.706 ± 0.314
2.105TyrGlu: 2.105 ± 0.268
1.761TyrPhe: 1.761 ± 0.265
2.406TyrGly: 2.406 ± 0.368
0.988TyrHis: 0.988 ± 0.205
2.792TyrIle: 2.792 ± 0.4
2.663TyrLys: 2.663 ± 0.306
3.48TyrLeu: 3.48 ± 0.388
0.473TyrMet: 0.473 ± 0.155
1.589TyrAsn: 1.589 ± 0.275
1.117TyrPro: 1.117 ± 0.214
1.546TyrGln: 1.546 ± 0.265
1.546TyrArg: 1.546 ± 0.214
3.179TyrSer: 3.179 ± 0.33
1.718TyrThr: 1.718 ± 0.351
2.32TyrVal: 2.32 ± 0.35
0.387TyrTrp: 0.387 ± 0.119
1.461TyrTyr: 1.461 ± 0.258
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (23280 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski