Legionella waltersii
Average proteome isoelectric point is 6.73
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3346 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W1A4V5|A0A0W1A4V5_9GAMM Sporulation initiation inhibitor protein Soj OS=Legionella waltersii OX=66969 GN=parA PE=4 SV=1
MM1 pKa = 7.17 FSKK4 pKa = 11.12 YY5 pKa = 7.51 NTPRR9 pKa = 11.84 PDD11 pKa = 2.88 TSAVVGVVSCVAALATVLLLKK32 pKa = 10.79 AMSSEE37 pKa = 4.1 EE38 pKa = 4.28 VKK40 pKa = 10.91 FEE42 pKa = 4.64 PYY44 pKa = 10.59 LILNGTLDD52 pKa = 4.71 FYY54 pKa = 10.98 PSNDD58 pKa = 3.87 DD59 pKa = 4.25 YY60 pKa = 11.65 DD61 pKa = 3.82 IQSDD65 pKa = 4.72 DD66 pKa = 3.64 EE67 pKa = 5.7 DD68 pKa = 3.54 EE69 pKa = 4.71 TPFQFQFF76 pKa = 3.41
Molecular weight: 8.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.753
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.516
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.439
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.846
Patrickios 1.875
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.763
Protein with the highest isoelectric point:
>tr|A0A0W1ABZ8|A0A0W1ABZ8_9GAMM Major facilitator family transporter OS=Legionella waltersii OX=66969 GN=Lwal_1577 PE=4 SV=1
MM1 pKa = 6.78 SQARR5 pKa = 11.84 RR6 pKa = 11.84 LLALIQLLRR15 pKa = 11.84 QPRR18 pKa = 11.84 YY19 pKa = 9.58 AVSGQHH25 pKa = 6.75 LADD28 pKa = 3.56 TLGVSLRR35 pKa = 11.84 TILEE39 pKa = 4.2 RR40 pKa = 11.84 VKK42 pKa = 10.74 SVCAPHH48 pKa = 7.17 SRR50 pKa = 11.84 DD51 pKa = 3.42 SDD53 pKa = 3.82 LLRR56 pKa = 11.84 ADD58 pKa = 4.55 RR59 pKa = 11.84 EE60 pKa = 4.08 ATLRR64 pKa = 11.84 KK65 pKa = 9.57 RR66 pKa = 11.84 IKK68 pKa = 10.2 SANHH72 pKa = 4.81 QSIDD76 pKa = 3.63 EE77 pKa = 4.43 LKK79 pKa = 10.49 PLANALAMM87 pKa = 4.68
Molecular weight: 9.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.619
IPC_protein 10.687
Toseland 10.979
ProMoST 10.979
Dawson 11.023
Bjellqvist 10.818
Wikipedia 11.316
Rodwell 11.082
Grimsley 11.052
Solomon 11.286
Lehninger 11.242
Nozaki 10.965
DTASelect 10.818
Thurlkill 10.965
EMBOSS 11.418
Sillero 10.979
Patrickios 10.862
IPC_peptide 11.301
IPC2_peptide 10.043
IPC2.peptide.svr19 8.887
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3346
0
3346
1065030
50
3523
318.3
35.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.22 ± 0.044
1.169 ± 0.015
4.9 ± 0.032
6.03 ± 0.049
4.412 ± 0.032
5.998 ± 0.044
2.456 ± 0.021
7.268 ± 0.033
6.447 ± 0.041
10.877 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.461 ± 0.02
5.021 ± 0.035
4.07 ± 0.024
4.613 ± 0.038
4.121 ± 0.037
7.003 ± 0.035
5.258 ± 0.029
6.084 ± 0.043
1.123 ± 0.016
3.456 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here