Gyrovirus Tu243
Average proteome isoelectric point is 7.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|U5UAI8|U5UAI8_9VIRU CA1 OS=Gyrovirus Tu243 OX=1415627 PE=3 SV=1
MM1 pKa = 7.48 LADD4 pKa = 4.74 EE5 pKa = 5.29 LPGDD9 pKa = 4.08 AQASVQLRR17 pKa = 11.84 QFQEE21 pKa = 3.83 PLVPVGGEE29 pKa = 3.9 ACVGSCYY36 pKa = 10.42 GHH38 pKa = 6.34 SDD40 pKa = 3.53 RR41 pKa = 11.84 PISSEE46 pKa = 3.88 TSVWWFRR53 pKa = 11.84 HH54 pKa = 4.92 CRR56 pKa = 11.84 DD57 pKa = 2.69 WSEE60 pKa = 3.26 ARR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 KK65 pKa = 9.92 EE66 pKa = 3.17 ILEE69 pKa = 3.92 FFKK72 pKa = 10.78 VQKK75 pKa = 10.21 RR76 pKa = 11.84 RR77 pKa = 11.84 LPGVGGGLQEE87 pKa = 4.21 EE88 pKa = 4.87 LL89 pKa = 4.07
Molecular weight: 10.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.846
IPC2_protein 5.881
IPC_protein 5.881
Toseland 6.084
ProMoST 6.186
Dawson 6.122
Bjellqvist 6.097
Wikipedia 6.109
Rodwell 6.097
Grimsley 6.16
Solomon 6.122
Lehninger 6.109
Nozaki 6.351
DTASelect 6.532
Thurlkill 6.532
EMBOSS 6.504
Sillero 6.465
Patrickios 4.457
IPC_peptide 6.135
IPC2_peptide 6.453
IPC2.peptide.svr19 6.438
Protein with the highest isoelectric point:
>tr|U5UAI8|U5UAI8_9VIRU CA1 OS=Gyrovirus Tu243 OX=1415627 PE=3 SV=1
MM1 pKa = 6.57 VRR3 pKa = 11.84 YY4 pKa = 9.5 RR5 pKa = 11.84 RR6 pKa = 11.84 RR7 pKa = 11.84 RR8 pKa = 11.84 PWGRR12 pKa = 11.84 LYY14 pKa = 10.86 KK15 pKa = 9.68 WFRR18 pKa = 11.84 GGWHH22 pKa = 5.56 YY23 pKa = 11.06 KK24 pKa = 9.81 RR25 pKa = 11.84 RR26 pKa = 11.84 PKK28 pKa = 10.33 GRR30 pKa = 11.84 WYY32 pKa = 10.06 RR33 pKa = 11.84 SSWGRR38 pKa = 11.84 KK39 pKa = 6.44 RR40 pKa = 11.84 RR41 pKa = 11.84 YY42 pKa = 6.9 HH43 pKa = 4.8 HH44 pKa = 7.11 RR45 pKa = 11.84 YY46 pKa = 9.14 RR47 pKa = 11.84 RR48 pKa = 11.84 GLFGRR53 pKa = 11.84 NFKK56 pKa = 10.66 RR57 pKa = 11.84 SFYY60 pKa = 8.59 VQHH63 pKa = 6.88 PGSYY67 pKa = 7.84 TVRR70 pKa = 11.84 RR71 pKa = 11.84 TNPYY75 pKa = 7.3 NTQRR79 pKa = 11.84 LRR81 pKa = 11.84 FQGLILVNNAVTQTGTSSVSNMRR104 pKa = 11.84 ATAIEE109 pKa = 3.72 ISLRR113 pKa = 11.84 GLLLAYY119 pKa = 9.79 FMNHH123 pKa = 4.75 STGGPGWAGDD133 pKa = 3.72 SGTKK137 pKa = 10.02 VAISPLEE144 pKa = 3.79 WWRR147 pKa = 11.84 WAYY150 pKa = 9.93 IRR152 pKa = 11.84 IEE154 pKa = 4.1 PCNDD158 pKa = 2.2 GRR160 pKa = 11.84 VFGMAVPHH168 pKa = 7.52 DD169 pKa = 3.9 EE170 pKa = 4.53 VLYY173 pKa = 11.11 NWFCQWQLFKK183 pKa = 10.76 HH184 pKa = 5.74 VKK186 pKa = 8.69 TDD188 pKa = 3.62 LRR190 pKa = 11.84 VLNTDD195 pKa = 2.66 VRR197 pKa = 11.84 TSAEE201 pKa = 3.74 ARR203 pKa = 11.84 IVASLLVQDD212 pKa = 5.43 SYY214 pKa = 10.96 WQVGNRR220 pKa = 11.84 GATQLSQIKK229 pKa = 9.78 FPSFLEE235 pKa = 4.05 LSAWGAQWSFQPGLQSVSSRR255 pKa = 11.84 SFNHH259 pKa = 6.18 HH260 pKa = 6.2 SFVGTNDD267 pKa = 3.25 PMGEE271 pKa = 3.97 PWKK274 pKa = 10.7 VFCPLDD280 pKa = 4.05 NMTQQDD286 pKa = 3.98 TQISWVYY293 pKa = 9.29 ATLFLAEE300 pKa = 4.45 FASVTNKK307 pKa = 9.93 QGYY310 pKa = 7.24 NTEE313 pKa = 4.13 QTSLEE318 pKa = 4.08 MQALSDD324 pKa = 3.34 TWAVIKK330 pKa = 10.69 VRR332 pKa = 11.84 SEE334 pKa = 3.46 WMLGNQNRR342 pKa = 11.84 VKK344 pKa = 11.02 SNMVTFTTGEE354 pKa = 4.08 PPP356 pKa = 3.24
Molecular weight: 41.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.329
IPC2_protein 9.882
IPC_protein 10.745
Toseland 10.555
ProMoST 10.365
Dawson 10.73
Bjellqvist 10.482
Wikipedia 10.965
Rodwell 10.862
Grimsley 10.804
Solomon 10.818
Lehninger 10.774
Nozaki 10.54
DTASelect 10.482
Thurlkill 10.599
EMBOSS 10.979
Sillero 10.643
Patrickios 10.496
IPC_peptide 10.818
IPC2_peptide 9.531
IPC2.peptide.svr19 8.329
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
676
89
356
225.3
25.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.325 ± 0.093
1.775 ± 0.738
3.698 ± 0.645
5.621 ± 1.87
4.734 ± 0.233
9.911 ± 1.728
1.923 ± 0.49
3.254 ± 0.514
4.734 ± 1.009
7.544 ± 0.433
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.923 ± 0.454
3.994 ± 1.363
4.438 ± 0.357
5.178 ± 0.654
8.58 ± 1.159
8.136 ± 0.082
5.917 ± 1.439
6.657 ± 0.519
3.698 ± 0.995
2.959 ± 0.957
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here