Gyrovirus Tu243 
Average proteome isoelectric point is 7.35 
Get precalculated fractions of proteins 
 
  
    Acidic  
     
   
 
  
    pI < 6.8  
     
   
 
  
    6.8-7.4  
     
   
 
  
    pI > 7.4  
     
   
 
  
    Basic  
     
   
    
 
  
    All  
     
   
 
 
Note: above files contain also dissociation constants (pKa) 
 
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 
 Summary statistics related to proteome-wise predictions 
 
Protein with the lowest isoelectric point: 
>tr|U5UAI8|U5UAI8_9VIRU CA1 OS=Gyrovirus Tu243 OX=1415627 PE=3 SV=1 
MM1 pKa = 7.48  LADD4 pKa = 4.74  EE5 pKa = 5.29  LPGDD9 pKa = 4.08  AQASVQLRR17 pKa = 11.84  QFQEE21 pKa = 3.83  PLVPVGGEE29 pKa = 3.9  ACVGSCYY36 pKa = 10.42  GHH38 pKa = 6.34  SDD40 pKa = 3.53  RR41 pKa = 11.84  PISSEE46 pKa = 3.88  TSVWWFRR53 pKa = 11.84  HH54 pKa = 4.92  CRR56 pKa = 11.84  DD57 pKa = 2.69  WSEE60 pKa = 3.26  ARR62 pKa = 11.84  RR63 pKa = 11.84  RR64 pKa = 11.84  KK65 pKa = 9.92  EE66 pKa = 3.17  ILEE69 pKa = 3.92  FFKK72 pKa = 10.78  VQKK75 pKa = 10.21  RR76 pKa = 11.84  RR77 pKa = 11.84  LPGVGGGLQEE87 pKa = 4.21  EE88 pKa = 4.87  LL89 pKa = 4.07   
 Molecular weight: 10.12 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  5.846 
IPC2_protein 5.881 
IPC_protein 5.881 
Toseland    6.084 
ProMoST     6.186 
Dawson      6.122 
Bjellqvist  6.097 
Wikipedia   6.109 
Rodwell     6.097 
Grimsley    6.16 
Solomon     6.122 
Lehninger   6.109 
Nozaki      6.351 
DTASelect   6.532 
Thurlkill   6.532 
EMBOSS      6.504 
Sillero     6.465 
Patrickios  4.457 
IPC_peptide 6.135 
IPC2_peptide  6.453 
IPC2.peptide.svr19  6.438 
 Protein with the highest isoelectric point: 
>tr|U5UAI8|U5UAI8_9VIRU CA1 OS=Gyrovirus Tu243 OX=1415627 PE=3 SV=1 
MM1 pKa = 6.57  VRR3 pKa = 11.84  YY4 pKa = 9.5  RR5 pKa = 11.84  RR6 pKa = 11.84  RR7 pKa = 11.84  RR8 pKa = 11.84  PWGRR12 pKa = 11.84  LYY14 pKa = 10.86  KK15 pKa = 9.68  WFRR18 pKa = 11.84  GGWHH22 pKa = 5.56  YY23 pKa = 11.06  KK24 pKa = 9.81  RR25 pKa = 11.84  RR26 pKa = 11.84  PKK28 pKa = 10.33  GRR30 pKa = 11.84  WYY32 pKa = 10.06  RR33 pKa = 11.84  SSWGRR38 pKa = 11.84  KK39 pKa = 6.44  RR40 pKa = 11.84  RR41 pKa = 11.84  YY42 pKa = 6.9  HH43 pKa = 4.8  HH44 pKa = 7.11  RR45 pKa = 11.84  YY46 pKa = 9.14  RR47 pKa = 11.84  RR48 pKa = 11.84  GLFGRR53 pKa = 11.84  NFKK56 pKa = 10.66  RR57 pKa = 11.84  SFYY60 pKa = 8.59  VQHH63 pKa = 6.88  PGSYY67 pKa = 7.84  TVRR70 pKa = 11.84  RR71 pKa = 11.84  TNPYY75 pKa = 7.3  NTQRR79 pKa = 11.84  LRR81 pKa = 11.84  FQGLILVNNAVTQTGTSSVSNMRR104 pKa = 11.84  ATAIEE109 pKa = 3.72  ISLRR113 pKa = 11.84  GLLLAYY119 pKa = 9.79  FMNHH123 pKa = 4.75  STGGPGWAGDD133 pKa = 3.72  SGTKK137 pKa = 10.02  VAISPLEE144 pKa = 3.79  WWRR147 pKa = 11.84  WAYY150 pKa = 9.93  IRR152 pKa = 11.84  IEE154 pKa = 4.1  PCNDD158 pKa = 2.2  GRR160 pKa = 11.84  VFGMAVPHH168 pKa = 7.52  DD169 pKa = 3.9  EE170 pKa = 4.53  VLYY173 pKa = 11.11  NWFCQWQLFKK183 pKa = 10.76  HH184 pKa = 5.74  VKK186 pKa = 8.69  TDD188 pKa = 3.62  LRR190 pKa = 11.84  VLNTDD195 pKa = 2.66  VRR197 pKa = 11.84  TSAEE201 pKa = 3.74  ARR203 pKa = 11.84  IVASLLVQDD212 pKa = 5.43  SYY214 pKa = 10.96  WQVGNRR220 pKa = 11.84  GATQLSQIKK229 pKa = 9.78  FPSFLEE235 pKa = 4.05  LSAWGAQWSFQPGLQSVSSRR255 pKa = 11.84  SFNHH259 pKa = 6.18  HH260 pKa = 6.2  SFVGTNDD267 pKa = 3.25  PMGEE271 pKa = 3.97  PWKK274 pKa = 10.7  VFCPLDD280 pKa = 4.05  NMTQQDD286 pKa = 3.98  TQISWVYY293 pKa = 9.29  ATLFLAEE300 pKa = 4.45  FASVTNKK307 pKa = 9.93  QGYY310 pKa = 7.24  NTEE313 pKa = 4.13  QTSLEE318 pKa = 4.08  MQALSDD324 pKa = 3.34  TWAVIKK330 pKa = 10.69  VRR332 pKa = 11.84  SEE334 pKa = 3.46  WMLGNQNRR342 pKa = 11.84  VKK344 pKa = 11.02  SNMVTFTTGEE354 pKa = 4.08  PPP356 pKa = 3.24   
 Molecular weight: 41.61 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.329 
IPC2_protein 9.882 
IPC_protein 10.745 
Toseland    10.555 
ProMoST     10.365 
Dawson      10.73 
Bjellqvist  10.482 
Wikipedia   10.965 
Rodwell     10.862 
Grimsley    10.804 
Solomon     10.818 
Lehninger   10.774 
Nozaki      10.54 
DTASelect   10.482 
Thurlkill   10.599 
EMBOSS      10.979 
Sillero     10.643 
Patrickios  10.496 
IPC_peptide 10.818 
IPC2_peptide  9.531 
IPC2.peptide.svr19  8.329 
  
 
Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try ESI  
     
   
 
  
    ChTry ESI  
     
   
 
  
    ArgC ESI  
     
   
 
  
    LysN ESI  
     
   
 
  
    TryLysC ESI  
     
   
 
  
    Try MALDI  
     
   
 
  
    ChTry MALDI  
     
   
 
  
    ArgC MALDI  
     
   
 
  
    LysN MALDI  
     
   
 
  
    TryLysC MALDI  
     
   
 
  
    Try LTQ  
     
   
 
  
    ChTry LTQ  
     
   
 
  
    ArgC LTQ  
     
   
 
  
    LysN LTQ  
     
   
 
  
    TryLysC LTQ  
     
   
 
  
    Try MSlow  
     
   
 
  
    ChTry MSlow  
     
   
 
  
    ArgC MSlow  
     
   
 
  
    LysN MSlow  
     
   
 
  
    TryLysC MSlow  
     
   
 
  
    Try MShigh  
     
   
 
  
    ChTry MShigh  
     
   
 
  
    ArgC MShigh  
     
   
 
  
    LysN MShigh  
     
   
 
  
    TryLysC MShigh  
     
   
   
  
                       
General Statistics 
    
      
        Number of major isoforms
 
        Number of additional isoforms
 
        Number of all proteins
         
        Number of amino acids
 
        Min. Seq. Length
 
        Max. Seq. Length
 
        Avg. Seq. Length
 
        Avg. Mol. Weight
 
       
     
    
      
        3 
 
        
        0
 
        
        3 
         
        676
 
        89
 
        356
 
        225.3
 
        25.59
 
                
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
 
        Cys
 
        Asp
 
        Glu
 
        Phe
 
        Gly
 
        His
 
        Ile
 
        Lys
 
        Leu
 
       
     
    
                 
        5.325 ± 0.093
1.775 ± 0.738
 
        3.698 ± 0.645
5.621 ± 1.87
 
        4.734 ± 0.233
9.911 ± 1.728
 
        1.923 ± 0.49
3.254 ± 0.514
       
        4.734 ± 1.009
7.544 ± 0.433
         
                
     
  
  
  
      
          
        Met
         
        Asn
 
        Gln
 
        Pro
         
        Arg
 
        Ser
 
        Thr
 
        Val
 
        Trp
 
        Tyr
  
       
     
    
                 
        1.923 ± 0.454
3.994 ± 1.363
 
        4.438 ± 0.357
5.178 ± 0.654
 
        8.58 ± 1.159
8.136 ± 0.082
 
        5.917 ± 1.439
6.657 ± 0.519
       
        3.698 ± 0.995
2.959 ± 0.957
         
                
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level 
Most of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here