Sphingomonas jeddahensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas

Average proteome isoelectric point is 6.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2894 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V2ET61|A0A1V2ET61_9SPHN Uncharacterized protein OS=Sphingomonas jeddahensis OX=1915074 GN=SPHI_24410 PE=4 SV=1
MM1 pKa = 7.77EE2 pKa = 6.44IIKK5 pKa = 10.41QPIGEE10 pKa = 4.4AAPIDD15 pKa = 4.11GDD17 pKa = 3.96CIRR20 pKa = 11.84VQEE23 pKa = 4.15LEE25 pKa = 3.86GGRR28 pKa = 11.84FLLNGSVLLNCGDD41 pKa = 3.8VEE43 pKa = 4.22PAEE46 pKa = 4.47SVALVGGEE54 pKa = 4.29PYY56 pKa = 10.83ASYY59 pKa = 11.0DD60 pKa = 3.37AAEE63 pKa = 4.27AAGLAWANDD72 pKa = 3.28HH73 pKa = 6.1CTDD76 pKa = 3.3RR77 pKa = 11.84LYY79 pKa = 11.22VCRR82 pKa = 11.84SDD84 pKa = 3.7GTIPLPDD91 pKa = 3.38MVV93 pKa = 4.4

Molecular weight: 9.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V2EXV6|A0A1V2EXV6_9SPHN Putative HTH-type transcriptional regulator OS=Sphingomonas jeddahensis OX=1915074 GN=SPHI_07420 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84SVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.02KK41 pKa = 10.61LSAA44 pKa = 4.03

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2894

0

2894

925443

29

1540

319.8

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.89 ± 0.073

0.704 ± 0.014

5.949 ± 0.035

5.389 ± 0.046

3.409 ± 0.03

9.006 ± 0.041

1.974 ± 0.022

4.823 ± 0.027

2.819 ± 0.037

9.804 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.022

2.491 ± 0.033

5.388 ± 0.037

3.043 ± 0.023

7.671 ± 0.051

4.835 ± 0.029

5.464 ± 0.03

7.456 ± 0.034

1.422 ± 0.019

2.109 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski