Vitreoscilla filiformis
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3396 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A221KBU1|A0A221KBU1_VITFI Transposase OS=Vitreoscilla filiformis OX=63 GN=VITFI_CDS0715 PE=4 SV=1
MM1 pKa = 7.2 MSIAGWKK8 pKa = 8.51 VAQAEE13 pKa = 4.21 LAQASSDD20 pKa = 3.91 TILGSTATTASVSVTGSASSAVNTAGDD47 pKa = 3.7 QDD49 pKa = 3.26 WWKK52 pKa = 8.61 VTLSAGTTYY61 pKa = 10.3 TFKK64 pKa = 11.21 LNAGTGSTLDD74 pKa = 3.03 AYY76 pKa = 11.09 LRR78 pKa = 11.84 LLNSSGTQVGVNNDD92 pKa = 3.14 AATGTKK98 pKa = 9.64 NAQITVKK105 pKa = 9.62 ATVSGTYY112 pKa = 9.78 YY113 pKa = 10.96 LSAQGYY119 pKa = 8.97 NSTTGAYY126 pKa = 9.05 ALSVAATDD134 pKa = 3.71 TVLGTKK140 pKa = 8.7 ATTASVAVNGSASSTVDD157 pKa = 3.72 FAGDD161 pKa = 3.44 QDD163 pKa = 3.25 WWKK166 pKa = 8.61 VTLSAGTTYY175 pKa = 10.3 TFKK178 pKa = 11.21 LNAGTGSTLDD188 pKa = 3.03 AYY190 pKa = 11.09 LRR192 pKa = 11.84 LLNSSGTQVGVNNDD206 pKa = 3.14 AATGTKK212 pKa = 9.64 NAQITVKK219 pKa = 9.62 ATVSGTYY226 pKa = 9.78 YY227 pKa = 10.96 LSAQGYY233 pKa = 8.97 NSTTGAYY240 pKa = 9.05 ALSVAATDD248 pKa = 3.71 TVLGTKK254 pKa = 8.7 ATTASVAVNGSASSTVDD271 pKa = 3.72 FAGDD275 pKa = 3.44 QDD277 pKa = 3.25 WWKK280 pKa = 8.61 VTLSAGTTYY289 pKa = 10.3 TFKK292 pKa = 11.21 LNAGTGSTLDD302 pKa = 3.03 AYY304 pKa = 11.09 LRR306 pKa = 11.84 LLNSSGTQMLSNNDD320 pKa = 3.27 AATGTKK326 pKa = 9.59 NAQITFTATTSGTYY340 pKa = 10.13 YY341 pKa = 10.94 LSAQGNGTSTGAYY354 pKa = 8.81 ALSVVATDD362 pKa = 3.7 TVLGTTATTSSAVINGTASSTVDD385 pKa = 3.78 FAGDD389 pKa = 3.44 QDD391 pKa = 3.25 WWKK394 pKa = 8.61 VTLSAGTTYY403 pKa = 10.3 TFKK406 pKa = 11.21 LNAGTGSTLDD416 pKa = 3.03 AYY418 pKa = 11.09 LRR420 pKa = 11.84 LLNSSGTQVGANNDD434 pKa = 3.35 AATGDD439 pKa = 3.74 KK440 pKa = 10.35 NAQITVTATTSGTYY454 pKa = 10.13 YY455 pKa = 10.94 LSAQGNGTSTGAYY468 pKa = 8.81 ALSVVATDD476 pKa = 3.7 TVLGTTATTSSAVINGTASSTVDD499 pKa = 3.78 FAGDD503 pKa = 3.44 QDD505 pKa = 3.25 WWKK508 pKa = 8.61 VTLSAGTTYY517 pKa = 10.3 TFKK520 pKa = 11.21 LNAGTGSTLDD530 pKa = 3.03 AYY532 pKa = 11.09 LRR534 pKa = 11.84 LLNSSGTQVGANNDD548 pKa = 3.35 AATGDD553 pKa = 3.74 KK554 pKa = 10.35 NAQITVTATTSGTYY568 pKa = 10.13 YY569 pKa = 10.94 LSAQGNGTSTGAYY582 pKa = 8.81 ALSVVATDD590 pKa = 3.7 TVLGTTATTSSAVINGTASSTVDD613 pKa = 3.78 FAGDD617 pKa = 3.44 QDD619 pKa = 3.25 WWKK622 pKa = 8.61 VTLSAGTTYY631 pKa = 10.3 TFKK634 pKa = 11.21 LNAGTGSTLDD644 pKa = 3.03 AYY646 pKa = 11.09 LRR648 pKa = 11.84 LLNSSGTQVGANNDD662 pKa = 3.35 AATGDD667 pKa = 3.74 KK668 pKa = 10.35 NAQITVTATTSGTYY682 pKa = 10.13 YY683 pKa = 10.94 LSAQGNGTSTGAYY696 pKa = 8.81 ALSVVATDD704 pKa = 3.7 TVLGTTATTSSAVINGTASSTVDD727 pKa = 3.78 FAGDD731 pKa = 3.44 QDD733 pKa = 3.25 WWKK736 pKa = 8.61 VTLSAGTTYY745 pKa = 10.3 TFKK748 pKa = 11.21 LNAGTGSTLDD758 pKa = 3.03 AYY760 pKa = 11.09 LRR762 pKa = 11.84 LLNSSGTQVGANNDD776 pKa = 3.35 AATGDD781 pKa = 3.74 KK782 pKa = 10.35 NAQITVTATTSGTYY796 pKa = 10.13 YY797 pKa = 10.94 LSAQGNGTSTGAYY810 pKa = 8.81 ALSVVATDD818 pKa = 3.7 TVLGTTATTSSAVINGTASSTVDD841 pKa = 3.78 FAGDD845 pKa = 3.44 QDD847 pKa = 3.25 WWKK850 pKa = 8.61 VTLSAGTTYY859 pKa = 10.3 TFKK862 pKa = 11.21 LNAGTGSTLDD872 pKa = 3.03 AYY874 pKa = 11.09 LRR876 pKa = 11.84 LLNSSGTQVGANNDD890 pKa = 3.35 AATGDD895 pKa = 3.74 KK896 pKa = 10.35 NAQITVTATTSGTYY910 pKa = 10.13 YY911 pKa = 10.94 LSAQGNGTSTGAYY924 pKa = 8.81 ALSVVATDD932 pKa = 3.7 TVLGTTATTSSAVINGTASSTVDD955 pKa = 3.78 FAGDD959 pKa = 3.44 QDD961 pKa = 3.25 WWKK964 pKa = 8.61 VTLSAGTTYY973 pKa = 10.3 TFKK976 pKa = 11.21 LNAGTGSTLDD986 pKa = 3.03 AYY988 pKa = 11.09 LRR990 pKa = 11.84 LLNSSGTQVASNDD1003 pKa = 3.29 NATSGTSSAQITVTATANGTYY1024 pKa = 10.35 YY1025 pKa = 11.03 LAAQGSGGTTGAYY1038 pKa = 10.06 VLDD1041 pKa = 3.99 VLATDD1046 pKa = 4.79 TILGSTATTASVAVNGSSGSTVDD1069 pKa = 4.42 FAGDD1073 pKa = 3.44 QDD1075 pKa = 3.25 WWKK1078 pKa = 8.61 VTLSAGTTYY1087 pKa = 10.3 TFKK1090 pKa = 11.21 LNAGTGSVLDD1100 pKa = 3.94 AYY1102 pKa = 11.16 LRR1104 pKa = 11.84 VLNSSGTQVASNDD1117 pKa = 3.29 NATSGTSSAQITFTATANGTYY1138 pKa = 10.35 YY1139 pKa = 11.03 LAAQGSGSTTGAYY1152 pKa = 10.23 VLDD1155 pKa = 3.99 VLATDD1160 pKa = 4.79 TILGSTATTASVGINDD1176 pKa = 4.18 SANSTVDD1183 pKa = 3.38 YY1184 pKa = 10.95 AGEE1187 pKa = 3.91 QDD1189 pKa = 2.98 WWKK1192 pKa = 8.63 VTLSAGTTYY1201 pKa = 10.78 TFDD1204 pKa = 3.24 LKK1206 pKa = 11.11 ARR1208 pKa = 11.84 SGSLLDD1214 pKa = 3.82 AYY1216 pKa = 11.0 LRR1218 pKa = 11.84 LLDD1221 pKa = 4.07 SNGVQLKK1228 pKa = 10.74 VNDD1231 pKa = 4.12 AASDD1235 pKa = 3.93 GALDD1239 pKa = 3.72 AQITFTATRR1248 pKa = 11.84 NGTYY1252 pKa = 10.18 HH1253 pKa = 7.41 LSAQGASGTSGAYY1266 pKa = 9.42 VLSVQALDD1274 pKa = 3.54 AVKK1277 pKa = 10.7 GSTATTDD1284 pKa = 3.39 SVTLNATAVEE1294 pKa = 4.41 STVDD1298 pKa = 3.83 FAGDD1302 pKa = 3.44 QDD1304 pKa = 3.26 WWKK1307 pKa = 8.8 VTLTAGTTYY1316 pKa = 10.13 TFKK1319 pKa = 11.04 LGAGTDD1325 pKa = 3.9 STLDD1329 pKa = 2.91 AYY1331 pKa = 11.24 LRR1333 pKa = 11.84 VLSSTGALLSVNNDD1347 pKa = 3.01 ASSSTTDD1354 pKa = 3.3 AEE1356 pKa = 4.27 ITFTAGSSGTYY1367 pKa = 9.83 YY1368 pKa = 11.03 LSAQGNGTSTGAYY1381 pKa = 8.72 TLFATTDD1388 pKa = 3.75 TIVASSSTASIQAVNASASDD1408 pKa = 3.5 SAIDD1412 pKa = 4.34 FRR1414 pKa = 11.84 GDD1416 pKa = 3.27 QDD1418 pKa = 2.63 WWKK1421 pKa = 10.09 VSLVAGTTYY1430 pKa = 10.65 VFEE1433 pKa = 5.02 LKK1435 pKa = 10.66 ASAGSSLDD1443 pKa = 3.15 AFLRR1447 pKa = 11.84 LIDD1450 pKa = 4.75 DD1451 pKa = 4.02 SGSQVGARR1459 pKa = 11.84 DD1460 pKa = 3.78 GGFSSGTDD1468 pKa = 3.06 AKK1470 pKa = 9.69 ITYY1473 pKa = 7.66 TATTSGTYY1481 pKa = 10.09 YY1482 pKa = 10.87 LSAQGADD1489 pKa = 3.65 GTTGDD1494 pKa = 3.83 YY1495 pKa = 10.77 TVFATTDD1502 pKa = 4.57 LIAGSTEE1509 pKa = 3.97 TTSTVGLDD1517 pKa = 3.5 GTASTDD1523 pKa = 3.54 VIDD1526 pKa = 4.32 YY1527 pKa = 11.16 AGDD1530 pKa = 3.7 QDD1532 pKa = 3.62 WWTATLTAGTTYY1544 pKa = 10.78 VFYY1547 pKa = 11.04 LSASTDD1553 pKa = 3.39 SSLDD1557 pKa = 3.12 AYY1559 pKa = 11.05 LRR1561 pKa = 11.84 LLDD1564 pKa = 3.92 GNGAVLEE1571 pKa = 4.5 TNNNQSSDD1579 pKa = 3.32 TANARR1584 pKa = 11.84 VVYY1587 pKa = 9.63 EE1588 pKa = 4.08 ATQSGTYY1595 pKa = 9.32 YY1596 pKa = 10.89 LSAQGVSGSLGAYY1609 pKa = 10.03 DD1610 pKa = 3.71 VFTIVDD1616 pKa = 4.31 AVPGSTATTSEE1627 pKa = 4.56 LSTAPGAASSGTIDD1641 pKa = 4.31 YY1642 pKa = 7.79 GTDD1645 pKa = 3.27 QDD1647 pKa = 3.0 WWKK1650 pKa = 8.33 ITLVAGRR1657 pKa = 11.84 TYY1659 pKa = 10.03 TFRR1662 pKa = 11.84 LGADD1666 pKa = 2.97 VDD1668 pKa = 4.59 SPLDD1672 pKa = 3.57 AYY1674 pKa = 10.99 LRR1676 pKa = 11.84 LVDD1679 pKa = 4.7 QDD1681 pKa = 3.75 GTQLAAGNDD1690 pKa = 3.77 STNGSVASKK1699 pKa = 10.15 DD1700 pKa = 3.34 ARR1702 pKa = 11.84 VTYY1705 pKa = 8.76 TATYY1709 pKa = 9.13 SGTYY1713 pKa = 8.65 YY1714 pKa = 10.85 VSAQGYY1720 pKa = 8.94 SGSEE1724 pKa = 3.9 GNYY1727 pKa = 9.18 VMSVGSDD1734 pKa = 3.56 AIDD1737 pKa = 3.85 GSVNTTASLTVNGAAGSSDD1756 pKa = 2.94 IDD1758 pKa = 3.67 EE1759 pKa = 5.43 SADD1762 pKa = 3.5 QDD1764 pKa = 2.68 WWRR1767 pKa = 11.84 IRR1769 pKa = 11.84 LSAGSSYY1776 pKa = 8.94 TFRR1779 pKa = 11.84 LTAPSGSSLDD1789 pKa = 3.47 PYY1791 pKa = 11.21 LRR1793 pKa = 11.84 LLNSTGVEE1801 pKa = 3.82 LVVNDD1806 pKa = 4.67 DD1807 pKa = 4.61 SGSSTQDD1814 pKa = 3.07 SLISFTATATGVYY1827 pKa = 9.76 YY1828 pKa = 10.87 LSAQGAGTTFGSYY1841 pKa = 10.48 NIQAFGDD1848 pKa = 4.67 AIAGSTDD1855 pKa = 3.0 TTAVLTMNTTGTSTIDD1871 pKa = 3.35 TVGDD1875 pKa = 3.53 QDD1877 pKa = 2.98 WWKK1880 pKa = 8.67 VTLTAGTAYY1889 pKa = 9.89 EE1890 pKa = 4.35 FQLQAADD1897 pKa = 3.99 GSSIDD1902 pKa = 3.63 AYY1904 pKa = 11.17 LRR1906 pKa = 11.84 LLNASGNLVTFNDD1919 pKa = 4.29 DD1920 pKa = 3.4 NGSLRR1925 pKa = 11.84 DD1926 pKa = 3.54 SLLIYY1931 pKa = 10.34 TPTTSGTYY1939 pKa = 9.85 YY1940 pKa = 10.89 LSAQSRR1946 pKa = 11.84 DD1947 pKa = 3.3 VTGDD1951 pKa = 3.51 YY1952 pKa = 10.58 VISAMASDD1960 pKa = 5.77 AIPGTIEE1967 pKa = 3.86 STVTLDD1973 pKa = 3.36 VNGVAGASVVDD1984 pKa = 4.76 LATDD1988 pKa = 3.52 QDD1990 pKa = 3.09 WWKK1993 pKa = 8.77 ITLTAGATYY2002 pKa = 9.55 TFLMKK2007 pKa = 10.18 PQTGSTLDD2015 pKa = 3.37 PYY2017 pKa = 10.95 LRR2019 pKa = 11.84 LMNSEE2024 pKa = 3.98 GQEE2027 pKa = 3.79 LASNNDD2033 pKa = 3.59 RR2034 pKa = 11.84 NGSTVNAYY2042 pKa = 7.93 ITYY2045 pKa = 8.06 TATTSGTYY2053 pKa = 10.24 FLAAQSNSGASGDD2066 pKa = 4.05 ALAGLYY2072 pKa = 8.43 TIKK2075 pKa = 10.8 ANSVDD2080 pKa = 4.24 IIDD2083 pKa = 4.48 GANTTTSVLVISGAEE2098 pKa = 4.04 GSSMIDD2104 pKa = 3.2 SSTDD2108 pKa = 3.25 QDD2110 pKa = 2.8 WWKK2113 pKa = 11.21 VEE2115 pKa = 4.0 LTAGTAYY2122 pKa = 9.64 TFRR2125 pKa = 11.84 LSAAADD2131 pKa = 3.9 SPLDD2135 pKa = 3.57 AYY2137 pKa = 11.11 LRR2139 pKa = 11.84 LLNSDD2144 pKa = 3.69 GTAVASNDD2152 pKa = 3.65 DD2153 pKa = 3.94 SGSSKK2158 pKa = 10.95 NSVISYY2164 pKa = 6.36 TAKK2167 pKa = 7.45 TTGVFYY2173 pKa = 10.85 LSAQGYY2179 pKa = 7.54 GASTGAYY2186 pKa = 8.99 VISATSTASDD2196 pKa = 4.39 SIPGTRR2202 pKa = 11.84 ATRR2205 pKa = 11.84 AVLTVGGDD2213 pKa = 3.47 AGSSTVDD2220 pKa = 3.08 ATSDD2224 pKa = 3.48 QDD2226 pKa = 2.81 WWKK2229 pKa = 8.34 VTLVSGTTYY2238 pKa = 10.8 TFDD2241 pKa = 3.94 LKK2243 pKa = 11.08 AAADD2247 pKa = 3.94 SSLDD2251 pKa = 3.38 SYY2253 pKa = 11.8 LRR2255 pKa = 11.84 LLDD2258 pKa = 3.89 SNGVLLTYY2266 pKa = 10.95 NDD2268 pKa = 4.74 DD2269 pKa = 3.98 NNGADD2274 pKa = 4.09 DD2275 pKa = 3.95 AQIIYY2280 pKa = 8.08 TATKK2284 pKa = 9.81 SGTFYY2289 pKa = 11.18 LSAQGYY2295 pKa = 8.82 GSSTGAYY2302 pKa = 10.16 SLTATANAADD2312 pKa = 5.06 TIPGSASTTATLTVNGEE2329 pKa = 4.27 AGSSAIDD2336 pKa = 3.46 STGDD2340 pKa = 3.33 QDD2342 pKa = 2.82 WWKK2345 pKa = 10.45 VRR2347 pKa = 11.84 LKK2349 pKa = 11.21 VGVTYY2354 pKa = 10.98 VFTLSADD2361 pKa = 3.37 VDD2363 pKa = 4.11 STLDD2367 pKa = 3.3 PTLRR2371 pKa = 11.84 LLNSAGTKK2379 pKa = 8.46 LTSNDD2384 pKa = 5.21 DD2385 pKa = 3.67 INDD2388 pKa = 3.57 SNKK2391 pKa = 10.0 NSRR2394 pKa = 11.84 ITYY2397 pKa = 6.48 TASATGTYY2405 pKa = 9.85 YY2406 pKa = 11.19 LSAQGYY2412 pKa = 8.9 NSTGAYY2418 pKa = 7.8 TIAVVSSGAASLAQAASEE2436 pKa = 4.53 TSTSTPDD2443 pKa = 3.13 AEE2445 pKa = 4.5 VFSFTATRR2453 pKa = 11.84 SEE2455 pKa = 4.13 VTTIRR2460 pKa = 11.84 DD2461 pKa = 3.3 FDD2463 pKa = 3.79 TGTDD2467 pKa = 3.57 RR2468 pKa = 11.84 IEE2470 pKa = 4.09 VTSSNFGGLKK2480 pKa = 10.22 VGALSAANFQAAGSTLTSRR2499 pKa = 11.84 AAVFLYY2505 pKa = 10.86 DD2506 pKa = 3.56 NTTGALAFDD2515 pKa = 4.62 ADD2517 pKa = 4.73 GIGAGAAVQIAQLTGARR2534 pKa = 11.84 TLRR2537 pKa = 11.84 ASDD2540 pKa = 3.18 IFVVNGG2546 pKa = 3.44
Molecular weight: 259.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.73
IPC2_protein 3.834
IPC_protein 3.923
Toseland 3.668
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.923
Rodwell 3.732
Grimsley 3.567
Solomon 3.935
Lehninger 3.897
Nozaki 4.037
DTASelect 4.393
Thurlkill 3.732
EMBOSS 3.923
Sillero 4.05
Patrickios 0.846
IPC_peptide 3.923
IPC2_peptide 4.024
IPC2.peptide.svr19 3.899
Protein with the highest isoelectric point:
>tr|A0A221KAB1|A0A221KAB1_VITFI Uncharacterized protein OS=Vitreoscilla filiformis OX=63 GN=VITFI_CDS0195 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.25 QPSKK9 pKa = 7.79 TRR11 pKa = 11.84 RR12 pKa = 11.84 ARR14 pKa = 11.84 THH16 pKa = 5.79 GFLVRR21 pKa = 11.84 MKK23 pKa = 10.53 SRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.94 GRR39 pKa = 11.84 KK40 pKa = 9.15 KK41 pKa = 9.63 LTQVV45 pKa = 2.87
Molecular weight: 5.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.413
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.34
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.076
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.034
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3396
0
3396
1110830
40
2641
327.1
35.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.671 ± 0.062
1.03 ± 0.015
5.218 ± 0.034
5.298 ± 0.037
3.256 ± 0.026
8.154 ± 0.052
2.59 ± 0.023
3.822 ± 0.036
2.929 ± 0.037
11.109 ± 0.062
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.331 ± 0.019
2.47 ± 0.036
5.552 ± 0.042
4.392 ± 0.035
7.109 ± 0.047
5.174 ± 0.039
5.38 ± 0.042
7.721 ± 0.037
1.755 ± 0.025
2.038 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here