Vitreoscilla filiformis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Vitreoscilla

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3396 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A221KBU1|A0A221KBU1_VITFI Transposase OS=Vitreoscilla filiformis OX=63 GN=VITFI_CDS0715 PE=4 SV=1
MM1 pKa = 7.2MSIAGWKK8 pKa = 8.51VAQAEE13 pKa = 4.21LAQASSDD20 pKa = 3.91TILGSTATTASVSVTGSASSAVNTAGDD47 pKa = 3.7QDD49 pKa = 3.26WWKK52 pKa = 8.61VTLSAGTTYY61 pKa = 10.3TFKK64 pKa = 11.21LNAGTGSTLDD74 pKa = 3.03AYY76 pKa = 11.09LRR78 pKa = 11.84LLNSSGTQVGVNNDD92 pKa = 3.14AATGTKK98 pKa = 9.64NAQITVKK105 pKa = 9.62ATVSGTYY112 pKa = 9.78YY113 pKa = 10.96LSAQGYY119 pKa = 8.97NSTTGAYY126 pKa = 9.05ALSVAATDD134 pKa = 3.71TVLGTKK140 pKa = 8.7ATTASVAVNGSASSTVDD157 pKa = 3.72FAGDD161 pKa = 3.44QDD163 pKa = 3.25WWKK166 pKa = 8.61VTLSAGTTYY175 pKa = 10.3TFKK178 pKa = 11.21LNAGTGSTLDD188 pKa = 3.03AYY190 pKa = 11.09LRR192 pKa = 11.84LLNSSGTQVGVNNDD206 pKa = 3.14AATGTKK212 pKa = 9.64NAQITVKK219 pKa = 9.62ATVSGTYY226 pKa = 9.78YY227 pKa = 10.96LSAQGYY233 pKa = 8.97NSTTGAYY240 pKa = 9.05ALSVAATDD248 pKa = 3.71TVLGTKK254 pKa = 8.7ATTASVAVNGSASSTVDD271 pKa = 3.72FAGDD275 pKa = 3.44QDD277 pKa = 3.25WWKK280 pKa = 8.61VTLSAGTTYY289 pKa = 10.3TFKK292 pKa = 11.21LNAGTGSTLDD302 pKa = 3.03AYY304 pKa = 11.09LRR306 pKa = 11.84LLNSSGTQMLSNNDD320 pKa = 3.27AATGTKK326 pKa = 9.59NAQITFTATTSGTYY340 pKa = 10.13YY341 pKa = 10.94LSAQGNGTSTGAYY354 pKa = 8.81ALSVVATDD362 pKa = 3.7TVLGTTATTSSAVINGTASSTVDD385 pKa = 3.78FAGDD389 pKa = 3.44QDD391 pKa = 3.25WWKK394 pKa = 8.61VTLSAGTTYY403 pKa = 10.3TFKK406 pKa = 11.21LNAGTGSTLDD416 pKa = 3.03AYY418 pKa = 11.09LRR420 pKa = 11.84LLNSSGTQVGANNDD434 pKa = 3.35AATGDD439 pKa = 3.74KK440 pKa = 10.35NAQITVTATTSGTYY454 pKa = 10.13YY455 pKa = 10.94LSAQGNGTSTGAYY468 pKa = 8.81ALSVVATDD476 pKa = 3.7TVLGTTATTSSAVINGTASSTVDD499 pKa = 3.78FAGDD503 pKa = 3.44QDD505 pKa = 3.25WWKK508 pKa = 8.61VTLSAGTTYY517 pKa = 10.3TFKK520 pKa = 11.21LNAGTGSTLDD530 pKa = 3.03AYY532 pKa = 11.09LRR534 pKa = 11.84LLNSSGTQVGANNDD548 pKa = 3.35AATGDD553 pKa = 3.74KK554 pKa = 10.35NAQITVTATTSGTYY568 pKa = 10.13YY569 pKa = 10.94LSAQGNGTSTGAYY582 pKa = 8.81ALSVVATDD590 pKa = 3.7TVLGTTATTSSAVINGTASSTVDD613 pKa = 3.78FAGDD617 pKa = 3.44QDD619 pKa = 3.25WWKK622 pKa = 8.61VTLSAGTTYY631 pKa = 10.3TFKK634 pKa = 11.21LNAGTGSTLDD644 pKa = 3.03AYY646 pKa = 11.09LRR648 pKa = 11.84LLNSSGTQVGANNDD662 pKa = 3.35AATGDD667 pKa = 3.74KK668 pKa = 10.35NAQITVTATTSGTYY682 pKa = 10.13YY683 pKa = 10.94LSAQGNGTSTGAYY696 pKa = 8.81ALSVVATDD704 pKa = 3.7TVLGTTATTSSAVINGTASSTVDD727 pKa = 3.78FAGDD731 pKa = 3.44QDD733 pKa = 3.25WWKK736 pKa = 8.61VTLSAGTTYY745 pKa = 10.3TFKK748 pKa = 11.21LNAGTGSTLDD758 pKa = 3.03AYY760 pKa = 11.09LRR762 pKa = 11.84LLNSSGTQVGANNDD776 pKa = 3.35AATGDD781 pKa = 3.74KK782 pKa = 10.35NAQITVTATTSGTYY796 pKa = 10.13YY797 pKa = 10.94LSAQGNGTSTGAYY810 pKa = 8.81ALSVVATDD818 pKa = 3.7TVLGTTATTSSAVINGTASSTVDD841 pKa = 3.78FAGDD845 pKa = 3.44QDD847 pKa = 3.25WWKK850 pKa = 8.61VTLSAGTTYY859 pKa = 10.3TFKK862 pKa = 11.21LNAGTGSTLDD872 pKa = 3.03AYY874 pKa = 11.09LRR876 pKa = 11.84LLNSSGTQVGANNDD890 pKa = 3.35AATGDD895 pKa = 3.74KK896 pKa = 10.35NAQITVTATTSGTYY910 pKa = 10.13YY911 pKa = 10.94LSAQGNGTSTGAYY924 pKa = 8.81ALSVVATDD932 pKa = 3.7TVLGTTATTSSAVINGTASSTVDD955 pKa = 3.78FAGDD959 pKa = 3.44QDD961 pKa = 3.25WWKK964 pKa = 8.61VTLSAGTTYY973 pKa = 10.3TFKK976 pKa = 11.21LNAGTGSTLDD986 pKa = 3.03AYY988 pKa = 11.09LRR990 pKa = 11.84LLNSSGTQVASNDD1003 pKa = 3.29NATSGTSSAQITVTATANGTYY1024 pKa = 10.35YY1025 pKa = 11.03LAAQGSGGTTGAYY1038 pKa = 10.06VLDD1041 pKa = 3.99VLATDD1046 pKa = 4.79TILGSTATTASVAVNGSSGSTVDD1069 pKa = 4.42FAGDD1073 pKa = 3.44QDD1075 pKa = 3.25WWKK1078 pKa = 8.61VTLSAGTTYY1087 pKa = 10.3TFKK1090 pKa = 11.21LNAGTGSVLDD1100 pKa = 3.94AYY1102 pKa = 11.16LRR1104 pKa = 11.84VLNSSGTQVASNDD1117 pKa = 3.29NATSGTSSAQITFTATANGTYY1138 pKa = 10.35YY1139 pKa = 11.03LAAQGSGSTTGAYY1152 pKa = 10.23VLDD1155 pKa = 3.99VLATDD1160 pKa = 4.79TILGSTATTASVGINDD1176 pKa = 4.18SANSTVDD1183 pKa = 3.38YY1184 pKa = 10.95AGEE1187 pKa = 3.91QDD1189 pKa = 2.98WWKK1192 pKa = 8.63VTLSAGTTYY1201 pKa = 10.78TFDD1204 pKa = 3.24LKK1206 pKa = 11.11ARR1208 pKa = 11.84SGSLLDD1214 pKa = 3.82AYY1216 pKa = 11.0LRR1218 pKa = 11.84LLDD1221 pKa = 4.07SNGVQLKK1228 pKa = 10.74VNDD1231 pKa = 4.12AASDD1235 pKa = 3.93GALDD1239 pKa = 3.72AQITFTATRR1248 pKa = 11.84NGTYY1252 pKa = 10.18HH1253 pKa = 7.41LSAQGASGTSGAYY1266 pKa = 9.42VLSVQALDD1274 pKa = 3.54AVKK1277 pKa = 10.7GSTATTDD1284 pKa = 3.39SVTLNATAVEE1294 pKa = 4.41STVDD1298 pKa = 3.83FAGDD1302 pKa = 3.44QDD1304 pKa = 3.26WWKK1307 pKa = 8.8VTLTAGTTYY1316 pKa = 10.13TFKK1319 pKa = 11.04LGAGTDD1325 pKa = 3.9STLDD1329 pKa = 2.91AYY1331 pKa = 11.24LRR1333 pKa = 11.84VLSSTGALLSVNNDD1347 pKa = 3.01ASSSTTDD1354 pKa = 3.3AEE1356 pKa = 4.27ITFTAGSSGTYY1367 pKa = 9.83YY1368 pKa = 11.03LSAQGNGTSTGAYY1381 pKa = 8.72TLFATTDD1388 pKa = 3.75TIVASSSTASIQAVNASASDD1408 pKa = 3.5SAIDD1412 pKa = 4.34FRR1414 pKa = 11.84GDD1416 pKa = 3.27QDD1418 pKa = 2.63WWKK1421 pKa = 10.09VSLVAGTTYY1430 pKa = 10.65VFEE1433 pKa = 5.02LKK1435 pKa = 10.66ASAGSSLDD1443 pKa = 3.15AFLRR1447 pKa = 11.84LIDD1450 pKa = 4.75DD1451 pKa = 4.02SGSQVGARR1459 pKa = 11.84DD1460 pKa = 3.78GGFSSGTDD1468 pKa = 3.06AKK1470 pKa = 9.69ITYY1473 pKa = 7.66TATTSGTYY1481 pKa = 10.09YY1482 pKa = 10.87LSAQGADD1489 pKa = 3.65GTTGDD1494 pKa = 3.83YY1495 pKa = 10.77TVFATTDD1502 pKa = 4.57LIAGSTEE1509 pKa = 3.97TTSTVGLDD1517 pKa = 3.5GTASTDD1523 pKa = 3.54VIDD1526 pKa = 4.32YY1527 pKa = 11.16AGDD1530 pKa = 3.7QDD1532 pKa = 3.62WWTATLTAGTTYY1544 pKa = 10.78VFYY1547 pKa = 11.04LSASTDD1553 pKa = 3.39SSLDD1557 pKa = 3.12AYY1559 pKa = 11.05LRR1561 pKa = 11.84LLDD1564 pKa = 3.92GNGAVLEE1571 pKa = 4.5TNNNQSSDD1579 pKa = 3.32TANARR1584 pKa = 11.84VVYY1587 pKa = 9.63EE1588 pKa = 4.08ATQSGTYY1595 pKa = 9.32YY1596 pKa = 10.89LSAQGVSGSLGAYY1609 pKa = 10.03DD1610 pKa = 3.71VFTIVDD1616 pKa = 4.31AVPGSTATTSEE1627 pKa = 4.56LSTAPGAASSGTIDD1641 pKa = 4.31YY1642 pKa = 7.79GTDD1645 pKa = 3.27QDD1647 pKa = 3.0WWKK1650 pKa = 8.33ITLVAGRR1657 pKa = 11.84TYY1659 pKa = 10.03TFRR1662 pKa = 11.84LGADD1666 pKa = 2.97VDD1668 pKa = 4.59SPLDD1672 pKa = 3.57AYY1674 pKa = 10.99LRR1676 pKa = 11.84LVDD1679 pKa = 4.7QDD1681 pKa = 3.75GTQLAAGNDD1690 pKa = 3.77STNGSVASKK1699 pKa = 10.15DD1700 pKa = 3.34ARR1702 pKa = 11.84VTYY1705 pKa = 8.76TATYY1709 pKa = 9.13SGTYY1713 pKa = 8.65YY1714 pKa = 10.85VSAQGYY1720 pKa = 8.94SGSEE1724 pKa = 3.9GNYY1727 pKa = 9.18VMSVGSDD1734 pKa = 3.56AIDD1737 pKa = 3.85GSVNTTASLTVNGAAGSSDD1756 pKa = 2.94IDD1758 pKa = 3.67EE1759 pKa = 5.43SADD1762 pKa = 3.5QDD1764 pKa = 2.68WWRR1767 pKa = 11.84IRR1769 pKa = 11.84LSAGSSYY1776 pKa = 8.94TFRR1779 pKa = 11.84LTAPSGSSLDD1789 pKa = 3.47PYY1791 pKa = 11.21LRR1793 pKa = 11.84LLNSTGVEE1801 pKa = 3.82LVVNDD1806 pKa = 4.67DD1807 pKa = 4.61SGSSTQDD1814 pKa = 3.07SLISFTATATGVYY1827 pKa = 9.76YY1828 pKa = 10.87LSAQGAGTTFGSYY1841 pKa = 10.48NIQAFGDD1848 pKa = 4.67AIAGSTDD1855 pKa = 3.0TTAVLTMNTTGTSTIDD1871 pKa = 3.35TVGDD1875 pKa = 3.53QDD1877 pKa = 2.98WWKK1880 pKa = 8.67VTLTAGTAYY1889 pKa = 9.89EE1890 pKa = 4.35FQLQAADD1897 pKa = 3.99GSSIDD1902 pKa = 3.63AYY1904 pKa = 11.17LRR1906 pKa = 11.84LLNASGNLVTFNDD1919 pKa = 4.29DD1920 pKa = 3.4NGSLRR1925 pKa = 11.84DD1926 pKa = 3.54SLLIYY1931 pKa = 10.34TPTTSGTYY1939 pKa = 9.85YY1940 pKa = 10.89LSAQSRR1946 pKa = 11.84DD1947 pKa = 3.3VTGDD1951 pKa = 3.51YY1952 pKa = 10.58VISAMASDD1960 pKa = 5.77AIPGTIEE1967 pKa = 3.86STVTLDD1973 pKa = 3.36VNGVAGASVVDD1984 pKa = 4.76LATDD1988 pKa = 3.52QDD1990 pKa = 3.09WWKK1993 pKa = 8.77ITLTAGATYY2002 pKa = 9.55TFLMKK2007 pKa = 10.18PQTGSTLDD2015 pKa = 3.37PYY2017 pKa = 10.95LRR2019 pKa = 11.84LMNSEE2024 pKa = 3.98GQEE2027 pKa = 3.79LASNNDD2033 pKa = 3.59RR2034 pKa = 11.84NGSTVNAYY2042 pKa = 7.93ITYY2045 pKa = 8.06TATTSGTYY2053 pKa = 10.24FLAAQSNSGASGDD2066 pKa = 4.05ALAGLYY2072 pKa = 8.43TIKK2075 pKa = 10.8ANSVDD2080 pKa = 4.24IIDD2083 pKa = 4.48GANTTTSVLVISGAEE2098 pKa = 4.04GSSMIDD2104 pKa = 3.2SSTDD2108 pKa = 3.25QDD2110 pKa = 2.8WWKK2113 pKa = 11.21VEE2115 pKa = 4.0LTAGTAYY2122 pKa = 9.64TFRR2125 pKa = 11.84LSAAADD2131 pKa = 3.9SPLDD2135 pKa = 3.57AYY2137 pKa = 11.11LRR2139 pKa = 11.84LLNSDD2144 pKa = 3.69GTAVASNDD2152 pKa = 3.65DD2153 pKa = 3.94SGSSKK2158 pKa = 10.95NSVISYY2164 pKa = 6.36TAKK2167 pKa = 7.45TTGVFYY2173 pKa = 10.85LSAQGYY2179 pKa = 7.54GASTGAYY2186 pKa = 8.99VISATSTASDD2196 pKa = 4.39SIPGTRR2202 pKa = 11.84ATRR2205 pKa = 11.84AVLTVGGDD2213 pKa = 3.47AGSSTVDD2220 pKa = 3.08ATSDD2224 pKa = 3.48QDD2226 pKa = 2.81WWKK2229 pKa = 8.34VTLVSGTTYY2238 pKa = 10.8TFDD2241 pKa = 3.94LKK2243 pKa = 11.08AAADD2247 pKa = 3.94SSLDD2251 pKa = 3.38SYY2253 pKa = 11.8LRR2255 pKa = 11.84LLDD2258 pKa = 3.89SNGVLLTYY2266 pKa = 10.95NDD2268 pKa = 4.74DD2269 pKa = 3.98NNGADD2274 pKa = 4.09DD2275 pKa = 3.95AQIIYY2280 pKa = 8.08TATKK2284 pKa = 9.81SGTFYY2289 pKa = 11.18LSAQGYY2295 pKa = 8.82GSSTGAYY2302 pKa = 10.16SLTATANAADD2312 pKa = 5.06TIPGSASTTATLTVNGEE2329 pKa = 4.27AGSSAIDD2336 pKa = 3.46STGDD2340 pKa = 3.33QDD2342 pKa = 2.82WWKK2345 pKa = 10.45VRR2347 pKa = 11.84LKK2349 pKa = 11.21VGVTYY2354 pKa = 10.98VFTLSADD2361 pKa = 3.37VDD2363 pKa = 4.11STLDD2367 pKa = 3.3PTLRR2371 pKa = 11.84LLNSAGTKK2379 pKa = 8.46LTSNDD2384 pKa = 5.21DD2385 pKa = 3.67INDD2388 pKa = 3.57SNKK2391 pKa = 10.0NSRR2394 pKa = 11.84ITYY2397 pKa = 6.48TASATGTYY2405 pKa = 9.85YY2406 pKa = 11.19LSAQGYY2412 pKa = 8.9NSTGAYY2418 pKa = 7.8TIAVVSSGAASLAQAASEE2436 pKa = 4.53TSTSTPDD2443 pKa = 3.13AEE2445 pKa = 4.5VFSFTATRR2453 pKa = 11.84SEE2455 pKa = 4.13VTTIRR2460 pKa = 11.84DD2461 pKa = 3.3FDD2463 pKa = 3.79TGTDD2467 pKa = 3.57RR2468 pKa = 11.84IEE2470 pKa = 4.09VTSSNFGGLKK2480 pKa = 10.22VGALSAANFQAAGSTLTSRR2499 pKa = 11.84AAVFLYY2505 pKa = 10.86DD2506 pKa = 3.56NTTGALAFDD2515 pKa = 4.62ADD2517 pKa = 4.73GIGAGAAVQIAQLTGARR2534 pKa = 11.84TLRR2537 pKa = 11.84ASDD2540 pKa = 3.18IFVVNGG2546 pKa = 3.44

Molecular weight:
259.55 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A221KAB1|A0A221KAB1_VITFI Uncharacterized protein OS=Vitreoscilla filiformis OX=63 GN=VITFI_CDS0195 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 10.53SRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 9.15KK41 pKa = 9.63LTQVV45 pKa = 2.87

Molecular weight:
5.25 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3396

0

3396

1110830

40

2641

327.1

35.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.671 ± 0.062

1.03 ± 0.015

5.218 ± 0.034

5.298 ± 0.037

3.256 ± 0.026

8.154 ± 0.052

2.59 ± 0.023

3.822 ± 0.036

2.929 ± 0.037

11.109 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.331 ± 0.019

2.47 ± 0.036

5.552 ± 0.042

4.392 ± 0.035

7.109 ± 0.047

5.174 ± 0.039

5.38 ± 0.042

7.721 ± 0.037

1.755 ± 0.025

2.038 ± 0.022

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski