Saccharothrix variisporea

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria;

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A495WZB3|A0A495WZB3_9PSEU Transcriptional regulator GlxA family with amidase domain OS=Saccharothrix variisporea OX=543527 GN=DFJ66_0200 PE=4 SV=1
MM1 pKa = 7.41NRR3 pKa = 11.84TLTLALVAAALCAVTTPTATAAALPAPAATVRR35 pKa = 11.84HH36 pKa = 6.95DD37 pKa = 5.25DD38 pKa = 4.21PDD40 pKa = 5.95DD41 pKa = 4.96GDD43 pKa = 3.31WEE45 pKa = 4.83YY46 pKa = 11.88YY47 pKa = 11.02GDD49 pKa = 3.71FHH51 pKa = 8.16NQVLCEE57 pKa = 3.89AAGAAGVVAGRR68 pKa = 11.84WDD70 pKa = 3.26EE71 pKa = 4.0WRR73 pKa = 11.84CDD75 pKa = 4.54GGDD78 pKa = 3.2LWVDD82 pKa = 3.56YY83 pKa = 10.66DD84 pKa = 4.82DD85 pKa = 6.35HH86 pKa = 9.2DD87 pKa = 6.33DD88 pKa = 5.07DD89 pKa = 5.62FPNSS93 pKa = 3.37

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A495XEL4|A0A495XEL4_9PSEU LmbE family N-acetylglucosaminyl deacetylase OS=Saccharothrix variisporea OX=543527 GN=DFJ66_5447 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84AKK17 pKa = 8.7THH19 pKa = 5.15GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AILAARR37 pKa = 11.84RR38 pKa = 11.84SKK40 pKa = 10.77GRR42 pKa = 11.84KK43 pKa = 7.91QLSAA47 pKa = 3.9

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8276

0

8276

2813303

29

7437

339.9

36.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.209 ± 0.04

0.75 ± 0.007

6.234 ± 0.024

5.406 ± 0.026

2.873 ± 0.017

9.16 ± 0.031

2.35 ± 0.015

2.811 ± 0.019

2.013 ± 0.021

10.5 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.504 ± 0.011

1.882 ± 0.019

6.029 ± 0.031

2.618 ± 0.014

8.02 ± 0.036

4.923 ± 0.017

6.301 ± 0.031

9.855 ± 0.028

1.603 ± 0.012

1.959 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski