Streptococcus satellite phage Javan337
Average proteome isoelectric point is 6.83
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 18 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZJV9|A0A4D5ZJV9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan337 OX=2558646 GN=JavanS337_0016 PE=4 SV=1
MM1 pKa = 7.91 DD2 pKa = 5.44 NDD4 pKa = 3.82 FKK6 pKa = 11.1 TVTNAKK12 pKa = 9.82 GLEE15 pKa = 3.76 IPKK18 pKa = 9.89 YY19 pKa = 10.4 FKK21 pKa = 10.62 DD22 pKa = 3.9 FKK24 pKa = 11.15 KK25 pKa = 10.36 LVEE28 pKa = 4.23 MDD30 pKa = 3.53 RR31 pKa = 11.84 QLAEE35 pKa = 4.31 YY36 pKa = 10.79 LCMNYY41 pKa = 10.13 EE42 pKa = 4.42 DD43 pKa = 5.91 LDD45 pKa = 4.41 SEE47 pKa = 4.46 DD48 pKa = 4.44 LGAFLEE54 pKa = 4.61 TVEE57 pKa = 5.78 QGFSWILDD65 pKa = 4.13 LIEE68 pKa = 5.5 SKK70 pKa = 11.04 DD71 pKa = 3.81 LLYY74 pKa = 11.01 SPQAGKK80 pKa = 10.39 KK81 pKa = 9.23 AA82 pKa = 3.31
Molecular weight: 9.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.482
IPC2_protein 4.406
IPC_protein 4.304
Toseland 4.113
ProMoST 4.431
Dawson 4.266
Bjellqvist 4.418
Wikipedia 4.164
Rodwell 4.126
Grimsley 4.024
Solomon 4.253
Lehninger 4.202
Nozaki 4.38
DTASelect 4.558
Thurlkill 4.139
EMBOSS 4.177
Sillero 4.406
Patrickios 3.897
IPC_peptide 4.253
IPC2_peptide 4.393
IPC2.peptide.svr19 4.349
Protein with the highest isoelectric point:
>tr|A0A4D5ZKQ1|A0A4D5ZKQ1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan337 OX=2558646 GN=JavanS337_0011 PE=4 SV=1
MM1 pKa = 7.92 KK2 pKa = 9.25 ITEE5 pKa = 4.18 YY6 pKa = 10.54 KK7 pKa = 10.41 KK8 pKa = 10.18 KK9 pKa = 10.4 DD10 pKa = 3.2 GSVVYY15 pKa = 10.34 RR16 pKa = 11.84 SSVYY20 pKa = 10.71 LGIDD24 pKa = 3.15 TVTGKK29 pKa = 10.24 KK30 pKa = 10.26 VKK32 pKa = 8.24 TTISDD37 pKa = 3.44 RR38 pKa = 11.84 TKK40 pKa = 11.03 NRR42 pKa = 11.84 LKK44 pKa = 10.91 SKK46 pKa = 10.43 AIQAKK51 pKa = 9.85 VEE53 pKa = 4.13 FEE55 pKa = 4.56 KK56 pKa = 11.26 NGSTVTKK63 pKa = 8.73 TVNVTTYY70 pKa = 10.95 QEE72 pKa = 4.26 LTNLWLEE79 pKa = 4.53 NYY81 pKa = 9.36 CHH83 pKa = 5.63 TVKK86 pKa = 10.77 HH87 pKa = 5.55 STLIGAKK94 pKa = 10.28 NNIKK98 pKa = 10.37 KK99 pKa = 10.4 YY100 pKa = 10.16 LLPAFGDD107 pKa = 3.76 YY108 pKa = 10.88 KK109 pKa = 10.73 LDD111 pKa = 3.55 KK112 pKa = 9.47 LTPPTIQHH120 pKa = 5.94 QVNQWAIDD128 pKa = 3.66 YY129 pKa = 8.37 NQLGKK134 pKa = 10.84 GYY136 pKa = 8.08 QQYY139 pKa = 10.05 NQLHH143 pKa = 6.4 ALNKK147 pKa = 10.1 RR148 pKa = 11.84 ILSYY152 pKa = 10.8 AVSLQVIASNPASDD166 pKa = 3.88 IIVPRR171 pKa = 11.84 RR172 pKa = 11.84 KK173 pKa = 9.61 PKK175 pKa = 10.21 EE176 pKa = 4.01 GQKK179 pKa = 10.61 LKK181 pKa = 11.17 YY182 pKa = 10.43 LDD184 pKa = 4.8 DD185 pKa = 5.74 DD186 pKa = 4.06 NLKK189 pKa = 10.78 KK190 pKa = 10.68 FLDD193 pKa = 4.07 YY194 pKa = 11.17 LDD196 pKa = 4.36 QLPNTYY202 pKa = 10.72 KK203 pKa = 10.73 NFSDD207 pKa = 3.42 TVLYY211 pKa = 8.14 KK212 pKa = 10.44 TLLATGLRR220 pKa = 11.84 IRR222 pKa = 11.84 EE223 pKa = 4.12 CLALGWSDD231 pKa = 4.23 IDD233 pKa = 4.03 LQNGSISVTKK243 pKa = 9.71 TLNTLKK249 pKa = 10.49 EE250 pKa = 3.91 ITSPKK255 pKa = 8.96 SKK257 pKa = 10.77 SSIRR261 pKa = 11.84 EE262 pKa = 3.4 IALDD266 pKa = 3.68 TKK268 pKa = 9.91 TVLMLRR274 pKa = 11.84 LYY276 pKa = 10.42 KK277 pKa = 10.36 ARR279 pKa = 11.84 QSQLGRR285 pKa = 11.84 EE286 pKa = 3.96 IGVTFEE292 pKa = 4.95 KK293 pKa = 10.88 VFSDD297 pKa = 3.16 TFDD300 pKa = 3.36 NYY302 pKa = 10.94 RR303 pKa = 11.84 EE304 pKa = 4.03 AGALRR309 pKa = 11.84 FRR311 pKa = 11.84 LEE313 pKa = 3.84 KK314 pKa = 10.18 HH315 pKa = 6.13 LKK317 pKa = 10.08 LAGCPRR323 pKa = 11.84 LSFHH327 pKa = 7.61 AFRR330 pKa = 11.84 HH331 pKa = 4.58 THH333 pKa = 6.92 ASLLLNAGLPYY344 pKa = 10.43 KK345 pKa = 10.2 EE346 pKa = 3.68 IQTRR350 pKa = 11.84 LGHH353 pKa = 5.92 SKK355 pKa = 10.32 ISMTMDD361 pKa = 3.2 IYY363 pKa = 11.76 SHH365 pKa = 7.09 LSKK368 pKa = 10.92 DD369 pKa = 3.71 NKK371 pKa = 10.37 KK372 pKa = 10.32 KK373 pKa = 9.89 ATSFYY378 pKa = 10.66 EE379 pKa = 3.92 KK380 pKa = 10.27 AIEE383 pKa = 4.03 NLQSSS388 pKa = 4.18
Molecular weight: 44.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.039
IPC2_protein 9.209
IPC_protein 9.077
Toseland 9.897
ProMoST 9.56
Dawson 10.101
Bjellqvist 9.75
Wikipedia 10.248
Rodwell 10.628
Grimsley 10.175
Solomon 10.116
Lehninger 10.072
Nozaki 9.882
DTASelect 9.736
Thurlkill 9.94
EMBOSS 10.292
Sillero 10.014
Patrickios 10.028
IPC_peptide 10.116
IPC2_peptide 8.229
IPC2.peptide.svr19 8.13
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
18
0
18
2952
49
483
164.0
19.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.675 ± 0.428
0.44 ± 0.104
6.843 ± 0.549
8.537 ± 0.792
4.743 ± 0.421
4.641 ± 0.52
1.66 ± 0.29
6.741 ± 0.724
10.908 ± 0.669
10.23 ± 0.693
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.066 ± 0.319
5.42 ± 0.436
2.134 ± 0.297
4.099 ± 0.386
4.912 ± 0.644
5.522 ± 0.515
5.962 ± 0.617
5.352 ± 0.546
0.678 ± 0.129
4.438 ± 0.31
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here