Bizionia argentinensis JUB59
Average proteome isoelectric point is 6.45
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2926 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G2EGL8|G2EGL8_9FLAO tRNA (Guanine-N1)-methyltransferase OS=Bizionia argentinensis JUB59 OX=1046627 GN=BZARG_2276 PE=4 SV=1
MM1 pKa = 7.67 NLRR4 pKa = 11.84 KK5 pKa = 9.58 ILVLLLAVSSVITACKK21 pKa = 10.01 KK22 pKa = 10.77 DD23 pKa = 4.24 DD24 pKa = 4.78 DD25 pKa = 5.05 DD26 pKa = 6.95 GPMTVEE32 pKa = 4.13 LRR34 pKa = 11.84 DD35 pKa = 3.46 AAEE38 pKa = 4.49 VYY40 pKa = 10.74 AEE42 pKa = 4.66 DD43 pKa = 4.58 MIDD46 pKa = 3.22 IQDD49 pKa = 3.63 YY50 pKa = 11.37 LEE52 pKa = 4.03 THH54 pKa = 7.05 FYY56 pKa = 10.78 NYY58 pKa = 10.62 EE59 pKa = 3.91 EE60 pKa = 4.24 FQLNSAYY67 pKa = 10.98 SLANDD72 pKa = 3.25 NFKK75 pKa = 10.7 IVFDD79 pKa = 4.88 TIAGTNANKK88 pKa = 9.61 TPLIDD93 pKa = 3.67 MVDD96 pKa = 3.68 SKK98 pKa = 11.27 IVTQGGIDD106 pKa = 3.38 YY107 pKa = 8.64 TFYY110 pKa = 10.86 YY111 pKa = 10.74 LNVRR115 pKa = 11.84 EE116 pKa = 4.11 GLGNEE121 pKa = 3.64 IHH123 pKa = 7.09 RR124 pKa = 11.84 SDD126 pKa = 4.16 EE127 pKa = 3.66 ATLIYY132 pKa = 9.98 EE133 pKa = 4.86 GSSIPDD139 pKa = 3.06 NYY141 pKa = 10.68 VFDD144 pKa = 4.04 SSVNEE149 pKa = 4.11 SQFNLMAVGATSGVIRR165 pKa = 11.84 GFMEE169 pKa = 4.04 GVIEE173 pKa = 4.93 FKK175 pKa = 10.69 TSDD178 pKa = 3.55 SYY180 pKa = 11.89 DD181 pKa = 3.32 INGDD185 pKa = 3.39 GTVAYY190 pKa = 9.36 HH191 pKa = 5.19 NHH193 pKa = 6.88 GIGAAFIPSGIGYY206 pKa = 9.17 FNQPLAGVPSYY217 pKa = 10.95 TPLIFKK223 pKa = 10.4 FSLYY227 pKa = 10.45 KK228 pKa = 9.86 RR229 pKa = 11.84 TILDD233 pKa = 3.63 HH234 pKa = 7.42 DD235 pKa = 4.78 LDD237 pKa = 5.4 GIPSYY242 pKa = 11.42 LEE244 pKa = 4.18 DD245 pKa = 5.08 LNNNDD250 pKa = 5.63 DD251 pKa = 5.53 LFDD254 pKa = 6.13 DD255 pKa = 4.95 DD256 pKa = 4.68 TDD258 pKa = 4.31 GDD260 pKa = 3.86 QTPNFLDD267 pKa = 3.97 NDD269 pKa = 4.38 DD270 pKa = 5.67 DD271 pKa = 5.93 GDD273 pKa = 4.2 GYY275 pKa = 9.49 LTKK278 pKa = 10.85 DD279 pKa = 3.11 EE280 pKa = 4.99 LDD282 pKa = 3.4 YY283 pKa = 11.66 GEE285 pKa = 5.07 YY286 pKa = 10.17 VIQVGDD292 pKa = 3.75 SDD294 pKa = 3.86 PTFAANEE301 pKa = 4.21 FEE303 pKa = 4.4 SSRR306 pKa = 11.84 VEE308 pKa = 3.78 NNGQITIKK316 pKa = 9.95 TVILRR321 pKa = 11.84 DD322 pKa = 3.64 TNGNGTPDD330 pKa = 3.76 YY331 pKa = 10.79 LDD333 pKa = 3.51 EE334 pKa = 4.44 TSIPDD339 pKa = 3.24
Molecular weight: 37.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.77
IPC_protein 3.808
Toseland 3.579
ProMoST 3.961
Dawson 3.808
Bjellqvist 3.973
Wikipedia 3.757
Rodwell 3.63
Grimsley 3.49
Solomon 3.808
Lehninger 3.757
Nozaki 3.923
DTASelect 4.19
Thurlkill 3.63
EMBOSS 3.757
Sillero 3.935
Patrickios 1.354
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.843
Protein with the highest isoelectric point:
>tr|G2EG69|G2EG69_9FLAO Tetratricopeptide repeat protein OS=Bizionia argentinensis JUB59 OX=1046627 GN=BZARG_1903 PE=4 SV=1
MM1 pKa = 7.7 NIFLKK6 pKa = 10.63 PLLYY10 pKa = 9.41 FQKK13 pKa = 10.56 HH14 pKa = 5.12 GFHH17 pKa = 5.9 VCQRR21 pKa = 11.84 IADD24 pKa = 4.0 RR25 pKa = 11.84 MGIRR29 pKa = 11.84 AKK31 pKa = 9.99 VVRR34 pKa = 11.84 TSFMYY39 pKa = 9.36 LTFVTLGFGFAVYY52 pKa = 10.09 LFIAFWMRR60 pKa = 11.84 IKK62 pKa = 10.93 DD63 pKa = 3.65 LLFTKK68 pKa = 10.28 RR69 pKa = 11.84 SSVFDD74 pKa = 3.68 LL75 pKa = 4.35
Molecular weight: 8.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.314
IPC2_protein 9.882
IPC_protein 10.511
Toseland 10.628
ProMoST 10.292
Dawson 10.76
Bjellqvist 10.438
Wikipedia 10.935
Rodwell 11.125
Grimsley 10.818
Solomon 10.833
Lehninger 10.804
Nozaki 10.613
DTASelect 10.438
Thurlkill 10.643
EMBOSS 11.023
Sillero 10.672
Patrickios 10.906
IPC_peptide 10.833
IPC2_peptide 9.355
IPC2.peptide.svr19 8.43
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2926
0
2926
948185
34
4606
324.1
36.58
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.454 ± 0.037
0.714 ± 0.015
5.649 ± 0.031
6.523 ± 0.044
5.322 ± 0.04
6.109 ± 0.047
1.83 ± 0.018
8.264 ± 0.047
7.796 ± 0.057
9.378 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.296 ± 0.021
6.23 ± 0.039
3.253 ± 0.025
3.372 ± 0.027
3.32 ± 0.031
6.509 ± 0.031
5.837 ± 0.057
6.173 ± 0.036
0.95 ± 0.017
4.022 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here