Acinetobacter phage Acj61
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 241 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E5E492|E5E492_9CAUD Uncharacterized protein OS=Acinetobacter phage Acj61 OX=760732 GN=Acj61p111 PE=4 SV=1
MM1 pKa = 7.62 EE2 pKa = 4.86 MNLDD6 pKa = 3.27 ITHH9 pKa = 7.63 LLNEE13 pKa = 4.48 DD14 pKa = 3.27 SQYY17 pKa = 11.03 ATLPGGNNLFRR28 pKa = 11.84 AEE30 pKa = 4.14 IDD32 pKa = 3.59 PNFDD36 pKa = 3.59 LDD38 pKa = 3.81 YY39 pKa = 10.62 QFNVSEE45 pKa = 4.54 SSFGLQLTYY54 pKa = 8.35 EE55 pKa = 4.49 TKK57 pKa = 10.7 GG58 pKa = 3.27
Molecular weight: 6.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.91
IPC_protein 3.757
Toseland 3.592
ProMoST 3.948
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.605
Grimsley 3.516
Solomon 3.719
Lehninger 3.681
Nozaki 3.91
DTASelect 4.05
Thurlkill 3.668
EMBOSS 3.719
Sillero 3.884
Patrickios 1.875
IPC_peptide 3.719
IPC2_peptide 3.859
IPC2.peptide.svr19 3.806
Protein with the highest isoelectric point:
>tr|E5E4M0|E5E4M0_9CAUD Nucleoid disruption protein OS=Acinetobacter phage Acj61 OX=760732 GN=ndd PE=4 SV=1
MM1 pKa = 7.22 KK2 pKa = 9.77 VKK4 pKa = 10.65 SIMRR8 pKa = 11.84 VKK10 pKa = 10.63 DD11 pKa = 3.39 LRR13 pKa = 11.84 VVGAQRR19 pKa = 11.84 VAQFTSTGKK28 pKa = 10.16 LRR30 pKa = 11.84 EE31 pKa = 4.1 PGKK34 pKa = 10.58 NFALGLKK41 pKa = 8.12 PTADD45 pKa = 3.49 LSRR48 pKa = 11.84 PGFYY52 pKa = 10.71 FMVHH56 pKa = 5.75 PRR58 pKa = 11.84 KK59 pKa = 8.06 EE60 pKa = 3.96 TVYY63 pKa = 10.34 ARR65 pKa = 11.84 FYY67 pKa = 10.95 VGRR70 pKa = 11.84 QRR72 pKa = 11.84 SKK74 pKa = 10.78 QGFNNTASQLRR85 pKa = 11.84 LQRR88 pKa = 11.84 SSVGEE93 pKa = 4.18 VIMNAHH99 pKa = 5.97 VPFDD103 pKa = 3.89 VFFLPLEE110 pKa = 4.2 HH111 pKa = 6.75 MKK113 pKa = 10.49 PLTNGFMKK121 pKa = 10.71 GKK123 pKa = 9.76 FALLLTRR130 pKa = 11.84 SHH132 pKa = 6.96 NDD134 pKa = 2.57 RR135 pKa = 11.84 FQNLEE140 pKa = 3.78 EE141 pKa = 4.41 LNRR144 pKa = 11.84 MLSDD148 pKa = 3.15 NFKK151 pKa = 10.84 FYY153 pKa = 10.25 AQKK156 pKa = 10.7 YY157 pKa = 7.73
Molecular weight: 18.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.298
IPC2_protein 9.882
IPC_protein 10.57
Toseland 10.862
ProMoST 10.496
Dawson 10.935
Bjellqvist 10.613
Wikipedia 11.125
Rodwell 11.286
Grimsley 10.979
Solomon 11.038
Lehninger 11.008
Nozaki 10.818
DTASelect 10.613
Thurlkill 10.847
EMBOSS 11.242
Sillero 10.862
Patrickios 11.008
IPC_peptide 11.052
IPC2_peptide 9.282
IPC2.peptide.svr19 8.589
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
241
0
241
50364
30
1284
209.0
23.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.138 ± 0.199
1.052 ± 0.07
6.209 ± 0.121
6.789 ± 0.187
4.458 ± 0.119
6.119 ± 0.23
1.928 ± 0.089
7.12 ± 0.147
7.073 ± 0.194
7.895 ± 0.154
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.744 ± 0.092
5.639 ± 0.107
3.618 ± 0.093
3.665 ± 0.091
4.275 ± 0.094
6.145 ± 0.154
6.086 ± 0.216
6.761 ± 0.122
1.293 ± 0.068
3.995 ± 0.12
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here