Amino acid dipepetide frequency for Acinetobacter phage Acj61

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.964AlaAla: 4.964 ± 0.34
0.357AlaCys: 0.357 ± 0.086
4.11AlaAsp: 4.11 ± 0.303
4.428AlaGlu: 4.428 ± 0.341
2.581AlaPhe: 2.581 ± 0.226
4.607AlaGly: 4.607 ± 0.449
1.211AlaHis: 1.211 ± 0.174
5.56AlaIle: 5.56 ± 0.36
5.5AlaLys: 5.5 ± 0.346
6.513AlaLeu: 6.513 ± 0.359
2.025AlaMet: 2.025 ± 0.192
3.971AlaAsn: 3.971 ± 0.318
2.403AlaPro: 2.403 ± 0.248
3.157AlaGln: 3.157 ± 0.281
2.621AlaArg: 2.621 ± 0.21
4.507AlaSer: 4.507 ± 0.319
4.924AlaThr: 4.924 ± 0.515
4.507AlaVal: 4.507 ± 0.296
0.953AlaTrp: 0.953 ± 0.144
2.482AlaTyr: 2.482 ± 0.234
0.0AlaXaa: 0.0 ± 0.0
Cys
0.675CysAla: 0.675 ± 0.137
0.099CysCys: 0.099 ± 0.044
0.774CysAsp: 0.774 ± 0.126
0.635CysGlu: 0.635 ± 0.124
0.477CysPhe: 0.477 ± 0.113
0.715CysGly: 0.715 ± 0.114
0.357CysHis: 0.357 ± 0.096
0.576CysIle: 0.576 ± 0.1
0.655CysLys: 0.655 ± 0.117
0.655CysLeu: 0.655 ± 0.121
0.357CysMet: 0.357 ± 0.087
0.437CysAsn: 0.437 ± 0.094
0.477CysPro: 0.477 ± 0.096
0.417CysGln: 0.417 ± 0.101
0.536CysArg: 0.536 ± 0.099
0.735CysSer: 0.735 ± 0.123
0.655CysThr: 0.655 ± 0.125
0.794CysVal: 0.794 ± 0.144
0.159CysTrp: 0.159 ± 0.054
0.338CysTyr: 0.338 ± 0.093
0.0CysXaa: 0.0 ± 0.0
Asp
4.428AspAla: 4.428 ± 0.322
0.635AspCys: 0.635 ± 0.1
3.594AspAsp: 3.594 ± 0.282
4.686AspGlu: 4.686 ± 0.344
2.978AspPhe: 2.978 ± 0.234
4.051AspGly: 4.051 ± 0.272
1.112AspHis: 1.112 ± 0.139
4.567AspIle: 4.567 ± 0.305
3.753AspLys: 3.753 ± 0.247
5.024AspLeu: 5.024 ± 0.311
1.509AspMet: 1.509 ± 0.143
3.256AspAsn: 3.256 ± 0.265
2.164AspPro: 2.164 ± 0.293
2.025AspGln: 2.025 ± 0.177
2.343AspArg: 2.343 ± 0.204
3.594AspSer: 3.594 ± 0.28
3.773AspThr: 3.773 ± 0.261
4.19AspVal: 4.19 ± 0.306
1.33AspTrp: 1.33 ± 0.16
3.078AspTyr: 3.078 ± 0.302
0.0AspXaa: 0.0 ± 0.0
Glu
4.607GluAla: 4.607 ± 0.363
1.013GluCys: 1.013 ± 0.154
3.951GluAsp: 3.951 ± 0.369
3.951GluGlu: 3.951 ± 0.307
3.534GluPhe: 3.534 ± 0.292
3.733GluGly: 3.733 ± 0.208
1.31GluHis: 1.31 ± 0.191
6.314GluIle: 6.314 ± 0.422
4.07GluLys: 4.07 ± 0.286
6.83GluLeu: 6.83 ± 0.372
2.105GluMet: 2.105 ± 0.189
3.276GluAsn: 3.276 ± 0.268
1.906GluPro: 1.906 ± 0.182
2.839GluGln: 2.839 ± 0.245
2.76GluArg: 2.76 ± 0.231
3.892GluSer: 3.892 ± 0.307
3.792GluThr: 3.792 ± 0.247
4.309GluVal: 4.309 ± 0.331
0.755GluTrp: 0.755 ± 0.142
2.939GluTyr: 2.939 ± 0.273
0.0GluXaa: 0.0 ± 0.0
Phe
2.959PheAla: 2.959 ± 0.234
0.338PheCys: 0.338 ± 0.095
3.832PheAsp: 3.832 ± 0.267
3.713PheGlu: 3.713 ± 0.321
1.529PhePhe: 1.529 ± 0.179
2.978PheGly: 2.978 ± 0.223
0.655PheHis: 0.655 ± 0.123
3.256PheIle: 3.256 ± 0.262
3.653PheLys: 3.653 ± 0.271
2.661PheLeu: 2.661 ± 0.21
1.35PheMet: 1.35 ± 0.172
2.72PheAsn: 2.72 ± 0.205
1.251PhePro: 1.251 ± 0.159
1.41PheGln: 1.41 ± 0.147
1.668PheArg: 1.668 ± 0.178
2.641PheSer: 2.641 ± 0.234
2.403PheThr: 2.403 ± 0.239
3.395PheVal: 3.395 ± 0.257
0.596PheTrp: 0.596 ± 0.109
1.569PheTyr: 1.569 ± 0.19
0.0PheXaa: 0.0 ± 0.0
Gly
4.408GlyAla: 4.408 ± 0.363
0.735GlyCys: 0.735 ± 0.116
3.475GlyAsp: 3.475 ± 0.322
3.594GlyGlu: 3.594 ± 0.265
2.8GlyPhe: 2.8 ± 0.297
3.495GlyGly: 3.495 ± 0.477
1.092GlyHis: 1.092 ± 0.168
4.19GlyIle: 4.19 ± 0.316
4.348GlyLys: 4.348 ± 0.285
4.885GlyLeu: 4.885 ± 0.315
1.787GlyMet: 1.787 ± 0.21
3.534GlyAsn: 3.534 ± 0.373
1.767GlyPro: 1.767 ± 0.201
2.164GlyGln: 2.164 ± 0.191
2.442GlyArg: 2.442 ± 0.226
4.309GlySer: 4.309 ± 0.335
4.229GlyThr: 4.229 ± 0.427
4.408GlyVal: 4.408 ± 0.341
0.834GlyTrp: 0.834 ± 0.143
2.7GlyTyr: 2.7 ± 0.238
0.0GlyXaa: 0.0 ± 0.0
His
1.072HisAla: 1.072 ± 0.143
0.278HisCys: 0.278 ± 0.082
1.41HisAsp: 1.41 ± 0.169
1.152HisGlu: 1.152 ± 0.142
1.033HisPhe: 1.033 ± 0.175
1.489HisGly: 1.489 ± 0.184
0.437HisHis: 0.437 ± 0.104
1.43HisIle: 1.43 ± 0.167
1.509HisLys: 1.509 ± 0.216
1.35HisLeu: 1.35 ± 0.14
0.377HisMet: 0.377 ± 0.095
1.112HisAsn: 1.112 ± 0.143
0.993HisPro: 0.993 ± 0.145
0.715HisGln: 0.715 ± 0.122
0.655HisArg: 0.655 ± 0.123
1.152HisSer: 1.152 ± 0.155
0.894HisThr: 0.894 ± 0.137
1.191HisVal: 1.191 ± 0.2
0.238HisTrp: 0.238 ± 0.076
0.794HisTyr: 0.794 ± 0.129
0.0HisXaa: 0.0 ± 0.0
Ile
5.679IleAla: 5.679 ± 0.419
0.874IleCys: 0.874 ± 0.135
5.024IleAsp: 5.024 ± 0.302
4.746IleGlu: 4.746 ± 0.343
2.72IlePhe: 2.72 ± 0.248
4.15IleGly: 4.15 ± 0.279
1.33IleHis: 1.33 ± 0.161
4.368IleIle: 4.368 ± 0.294
6.076IleLys: 6.076 ± 0.393
5.043IleLeu: 5.043 ± 0.352
1.708IleMet: 1.708 ± 0.195
4.607IleAsn: 4.607 ± 0.284
2.78IlePro: 2.78 ± 0.228
2.442IleGln: 2.442 ± 0.211
2.978IleArg: 2.978 ± 0.281
4.309IleSer: 4.309 ± 0.29
4.388IleThr: 4.388 ± 0.327
4.785IleVal: 4.785 ± 0.307
0.715IleTrp: 0.715 ± 0.107
2.482IleTyr: 2.482 ± 0.22
0.0IleXaa: 0.0 ± 0.0
Lys
5.46LysAla: 5.46 ± 0.34
0.596LysCys: 0.596 ± 0.106
4.11LysAsp: 4.11 ± 0.301
4.686LysGlu: 4.686 ± 0.342
3.713LysPhe: 3.713 ± 0.274
3.991LysGly: 3.991 ± 0.301
1.827LysHis: 1.827 ± 0.193
4.567LysIle: 4.567 ± 0.305
3.812LysLys: 3.812 ± 0.264
6.354LysLeu: 6.354 ± 0.338
2.343LysMet: 2.343 ± 0.217
3.693LysAsn: 3.693 ± 0.227
2.76LysPro: 2.76 ± 0.237
2.283LysGln: 2.283 ± 0.219
2.939LysArg: 2.939 ± 0.261
4.428LysSer: 4.428 ± 0.33
4.15LysThr: 4.15 ± 0.271
4.785LysVal: 4.785 ± 0.303
0.933LysTrp: 0.933 ± 0.122
3.296LysTyr: 3.296 ± 0.291
0.0LysXaa: 0.0 ± 0.0
Leu
6.036LeuAla: 6.036 ± 0.369
0.894LeuCys: 0.894 ± 0.135
4.924LeuAsp: 4.924 ± 0.339
5.937LeuGlu: 5.937 ± 0.401
3.018LeuPhe: 3.018 ± 0.233
4.229LeuGly: 4.229 ± 0.303
1.549LeuHis: 1.549 ± 0.196
5.262LeuIle: 5.262 ± 0.311
5.857LeuLys: 5.857 ± 0.351
5.163LeuLeu: 5.163 ± 0.36
2.204LeuMet: 2.204 ± 0.225
4.984LeuAsn: 4.984 ± 0.333
3.336LeuPro: 3.336 ± 0.277
3.495LeuGln: 3.495 ± 0.198
3.574LeuArg: 3.574 ± 0.292
4.964LeuSer: 4.964 ± 0.299
4.666LeuThr: 4.666 ± 0.304
4.746LeuVal: 4.746 ± 0.334
0.874LeuTrp: 0.874 ± 0.147
3.237LeuTyr: 3.237 ± 0.262
0.0LeuXaa: 0.0 ± 0.0
Met
2.363MetAla: 2.363 ± 0.204
0.238MetCys: 0.238 ± 0.077
1.628MetAsp: 1.628 ± 0.166
1.549MetGlu: 1.549 ± 0.19
1.013MetPhe: 1.013 ± 0.147
1.152MetGly: 1.152 ± 0.133
0.318MetHis: 0.318 ± 0.069
2.144MetIle: 2.144 ± 0.204
2.422MetLys: 2.422 ± 0.267
1.986MetLeu: 1.986 ± 0.189
0.596MetMet: 0.596 ± 0.119
2.085MetAsn: 2.085 ± 0.222
1.013MetPro: 1.013 ± 0.157
1.092MetGln: 1.092 ± 0.15
1.013MetArg: 1.013 ± 0.158
1.886MetSer: 1.886 ± 0.214
1.866MetThr: 1.866 ± 0.173
1.489MetVal: 1.489 ± 0.197
0.278MetTrp: 0.278 ± 0.064
1.31MetTyr: 1.31 ± 0.186
0.0MetXaa: 0.0 ± 0.0
Asn
4.249AsnAla: 4.249 ± 0.313
0.536AsnCys: 0.536 ± 0.122
3.395AsnAsp: 3.395 ± 0.232
3.574AsnGlu: 3.574 ± 0.229
2.74AsnPhe: 2.74 ± 0.239
4.19AsnGly: 4.19 ± 0.378
0.953AsnHis: 0.953 ± 0.128
4.09AsnIle: 4.09 ± 0.251
3.316AsnLys: 3.316 ± 0.265
4.567AsnLeu: 4.567 ± 0.328
1.608AsnMet: 1.608 ± 0.166
2.998AsnAsn: 2.998 ± 0.271
2.72AsnPro: 2.72 ± 0.248
1.628AsnGln: 1.628 ± 0.179
2.641AsnArg: 2.641 ± 0.187
3.435AsnSer: 3.435 ± 0.244
3.475AsnThr: 3.475 ± 0.289
3.792AsnVal: 3.792 ± 0.238
0.635AsnTrp: 0.635 ± 0.11
1.847AsnTyr: 1.847 ± 0.215
0.0AsnXaa: 0.0 ± 0.0
Pro
2.462ProAla: 2.462 ± 0.24
0.357ProCys: 0.357 ± 0.085
2.105ProAsp: 2.105 ± 0.219
2.839ProGlu: 2.839 ± 0.246
1.608ProPhe: 1.608 ± 0.191
2.522ProGly: 2.522 ± 0.218
0.596ProHis: 0.596 ± 0.13
2.283ProIle: 2.283 ± 0.236
2.839ProLys: 2.839 ± 0.234
2.323ProLeu: 2.323 ± 0.232
0.774ProMet: 0.774 ± 0.137
2.244ProAsn: 2.244 ± 0.208
1.152ProPro: 1.152 ± 0.174
1.33ProGln: 1.33 ± 0.163
1.648ProArg: 1.648 ± 0.179
2.184ProSer: 2.184 ± 0.208
2.244ProThr: 2.244 ± 0.217
2.7ProVal: 2.7 ± 0.274
0.477ProTrp: 0.477 ± 0.101
1.489ProTyr: 1.489 ± 0.17
0.0ProXaa: 0.0 ± 0.0
Gln
2.919GlnAla: 2.919 ± 0.271
0.477GlnCys: 0.477 ± 0.086
1.906GlnAsp: 1.906 ± 0.192
2.542GlnGlu: 2.542 ± 0.242
2.005GlnPhe: 2.005 ± 0.216
1.986GlnGly: 1.986 ± 0.19
0.596GlnHis: 0.596 ± 0.117
2.7GlnIle: 2.7 ± 0.244
2.343GlnLys: 2.343 ± 0.226
3.177GlnLeu: 3.177 ± 0.219
1.112GlnMet: 1.112 ± 0.163
1.588GlnAsn: 1.588 ± 0.174
1.291GlnPro: 1.291 ± 0.151
1.31GlnGln: 1.31 ± 0.206
1.906GlnArg: 1.906 ± 0.22
1.827GlnSer: 1.827 ± 0.176
2.442GlnThr: 2.442 ± 0.256
2.184GlnVal: 2.184 ± 0.213
0.596GlnTrp: 0.596 ± 0.109
1.747GlnTyr: 1.747 ± 0.192
0.0GlnXaa: 0.0 ± 0.0
Arg
2.899ArgAla: 2.899 ± 0.242
0.457ArgCys: 0.457 ± 0.099
2.502ArgAsp: 2.502 ± 0.207
3.117ArgGlu: 3.117 ± 0.279
2.184ArgPhe: 2.184 ± 0.209
2.442ArgGly: 2.442 ± 0.245
1.033ArgHis: 1.033 ± 0.133
3.336ArgIle: 3.336 ± 0.249
2.959ArgLys: 2.959 ± 0.228
3.594ArgLeu: 3.594 ± 0.232
1.013ArgMet: 1.013 ± 0.141
2.224ArgAsn: 2.224 ± 0.209
1.41ArgPro: 1.41 ± 0.153
1.727ArgGln: 1.727 ± 0.219
1.787ArgArg: 1.787 ± 0.22
2.82ArgSer: 2.82 ± 0.295
2.522ArgThr: 2.522 ± 0.252
2.681ArgVal: 2.681 ± 0.255
0.477ArgTrp: 0.477 ± 0.104
1.569ArgTyr: 1.569 ± 0.159
0.0ArgXaa: 0.0 ± 0.0
Ser
4.17SerAla: 4.17 ± 0.366
0.675SerCys: 0.675 ± 0.11
3.534SerAsp: 3.534 ± 0.293
3.852SerGlu: 3.852 ± 0.263
2.72SerPhe: 2.72 ± 0.232
4.249SerGly: 4.249 ± 0.37
1.31SerHis: 1.31 ± 0.197
4.209SerIle: 4.209 ± 0.281
4.507SerLys: 4.507 ± 0.315
5.024SerLeu: 5.024 ± 0.326
1.608SerMet: 1.608 ± 0.173
3.673SerAsn: 3.673 ± 0.33
1.906SerPro: 1.906 ± 0.194
2.204SerGln: 2.204 ± 0.188
2.601SerArg: 2.601 ± 0.217
4.11SerSer: 4.11 ± 0.381
3.753SerThr: 3.753 ± 0.322
4.408SerVal: 4.408 ± 0.307
0.715SerTrp: 0.715 ± 0.117
2.224SerTyr: 2.224 ± 0.256
0.0SerXaa: 0.0 ± 0.0
Thr
3.951ThrAla: 3.951 ± 0.481
0.596ThrCys: 0.596 ± 0.116
3.614ThrAsp: 3.614 ± 0.353
3.852ThrGlu: 3.852 ± 0.298
2.641ThrPhe: 2.641 ± 0.272
4.527ThrGly: 4.527 ± 0.382
1.132ThrHis: 1.132 ± 0.148
4.487ThrIle: 4.487 ± 0.38
3.912ThrLys: 3.912 ± 0.26
4.885ThrLeu: 4.885 ± 0.338
1.529ThrMet: 1.529 ± 0.176
3.415ThrAsn: 3.415 ± 0.277
2.879ThrPro: 2.879 ± 0.233
2.343ThrGln: 2.343 ± 0.23
2.859ThrArg: 2.859 ± 0.189
3.673ThrSer: 3.673 ± 0.314
3.475ThrThr: 3.475 ± 0.33
3.912ThrVal: 3.912 ± 0.332
0.854ThrTrp: 0.854 ± 0.16
2.323ThrTyr: 2.323 ± 0.239
0.0ThrXaa: 0.0 ± 0.0
Val
4.646ValAla: 4.646 ± 0.294
0.755ValCys: 0.755 ± 0.138
4.547ValAsp: 4.547 ± 0.363
5.599ValGlu: 5.599 ± 0.451
2.919ValPhe: 2.919 ± 0.246
3.495ValGly: 3.495 ± 0.295
1.37ValHis: 1.37 ± 0.192
4.805ValIle: 4.805 ± 0.322
5.083ValLys: 5.083 ± 0.345
5.024ValLeu: 5.024 ± 0.274
1.847ValMet: 1.847 ± 0.186
3.316ValAsn: 3.316 ± 0.24
2.045ValPro: 2.045 ± 0.238
2.383ValGln: 2.383 ± 0.241
3.316ValArg: 3.316 ± 0.262
3.812ValSer: 3.812 ± 0.25
4.09ValThr: 4.09 ± 0.318
5.302ValVal: 5.302 ± 0.388
0.616ValTrp: 0.616 ± 0.107
2.641ValTyr: 2.641 ± 0.213
0.0ValXaa: 0.0 ± 0.0
Trp
0.774TrpAla: 0.774 ± 0.093
0.238TrpCys: 0.238 ± 0.063
0.834TrpAsp: 0.834 ± 0.131
0.854TrpGlu: 0.854 ± 0.11
0.417TrpPhe: 0.417 ± 0.098
0.596TrpGly: 0.596 ± 0.118
0.397TrpHis: 0.397 ± 0.091
0.695TrpIle: 0.695 ± 0.139
1.152TrpLys: 1.152 ± 0.168
0.913TrpLeu: 0.913 ± 0.122
0.417TrpMet: 0.417 ± 0.069
0.913TrpAsn: 0.913 ± 0.121
0.357TrpPro: 0.357 ± 0.083
0.536TrpGln: 0.536 ± 0.1
0.457TrpArg: 0.457 ± 0.103
0.596TrpSer: 0.596 ± 0.1
0.933TrpThr: 0.933 ± 0.144
1.033TrpVal: 1.033 ± 0.152
0.278TrpTrp: 0.278 ± 0.077
0.536TrpTyr: 0.536 ± 0.101
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.661TyrAla: 2.661 ± 0.221
0.377TyrCys: 0.377 ± 0.095
2.82TyrAsp: 2.82 ± 0.212
2.581TyrGlu: 2.581 ± 0.212
1.946TyrPhe: 1.946 ± 0.189
2.601TyrGly: 2.601 ± 0.232
0.695TyrHis: 0.695 ± 0.12
2.363TyrIle: 2.363 ± 0.214
2.959TyrLys: 2.959 ± 0.274
2.978TyrLeu: 2.978 ± 0.231
1.152TyrMet: 1.152 ± 0.156
2.343TyrAsn: 2.343 ± 0.206
1.569TyrPro: 1.569 ± 0.193
1.152TyrGln: 1.152 ± 0.148
2.005TyrArg: 2.005 ± 0.186
2.581TyrSer: 2.581 ± 0.245
2.184TyrThr: 2.184 ± 0.211
2.998TyrVal: 2.998 ± 0.293
0.616TyrTrp: 0.616 ± 0.122
1.35TyrTyr: 1.35 ± 0.197
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 241 proteins (50364 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski