Lactobacillus phage phi jlb1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|X2CXW8|X2CXW8_9CAUD Peptidase_M78 domain-containing protein OS=Lactobacillus phage phi jlb1 OX=1416334 GN=jlb1_003 PE=4 SV=1
MM1 pKa = 7.74FDD3 pKa = 4.24FDD5 pKa = 4.79FSSIYY10 pKa = 10.98SNLEE14 pKa = 3.97SLCKK18 pKa = 10.25SSLDD22 pKa = 3.44NGYY25 pKa = 8.01FTDD28 pKa = 3.5NTIAGFVQQGTFDD41 pKa = 3.79ADD43 pKa = 3.7GYY45 pKa = 11.43KK46 pKa = 10.45RR47 pKa = 11.84ITGDD51 pKa = 3.57DD52 pKa = 3.91YY53 pKa = 12.05VAGDD57 pKa = 3.79QNTVANGG64 pKa = 3.19

Molecular weight:
7.01 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|X2CXM9|X2CXM9_9CAUD Uncharacterized protein OS=Lactobacillus phage phi jlb1 OX=1416334 GN=jlb1_010 PE=4 SV=1
MM1 pKa = 7.3KK2 pKa = 10.39RR3 pKa = 11.84EE4 pKa = 4.13LKK6 pKa = 10.24SLRR9 pKa = 11.84VGAGLTQAEE18 pKa = 4.32LAKK21 pKa = 10.61RR22 pKa = 11.84LGVTNVTVSRR32 pKa = 11.84WEE34 pKa = 3.89RR35 pKa = 11.84GEE37 pKa = 4.3TIPKK41 pKa = 9.06PKK43 pKa = 9.88YY44 pKa = 8.24IKK46 pKa = 10.98AMAKK50 pKa = 9.91LFNIKK55 pKa = 10.32GQDD58 pKa = 2.8IFLNLITTKK67 pKa = 10.32AYY69 pKa = 9.84KK70 pKa = 10.4VVTKK74 pKa = 10.85

Molecular weight:
8.36 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

11378

43

1136

210.7

23.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.725 ± 0.934

0.536 ± 0.129

7.312 ± 0.381

5.968 ± 0.539

3.401 ± 0.174

6.46 ± 0.588

1.266 ± 0.191

6.056 ± 0.293

8.552 ± 0.441

8.666 ± 0.402

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.435 ± 0.329

5.994 ± 0.44

2.997 ± 0.201

3.929 ± 0.172

4.025 ± 0.359

6.266 ± 0.239

6.205 ± 0.44

6.715 ± 0.404

1.318 ± 0.172

4.175 ± 0.429

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski