Lactobacillus phage phi jlb1
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|X2CXW8|X2CXW8_9CAUD Peptidase_M78 domain-containing protein OS=Lactobacillus phage phi jlb1 OX=1416334 GN=jlb1_003 PE=4 SV=1
MM1 pKa = 7.74 FDD3 pKa = 4.24 FDD5 pKa = 4.79 FSSIYY10 pKa = 10.98 SNLEE14 pKa = 3.97 SLCKK18 pKa = 10.25 SSLDD22 pKa = 3.44 NGYY25 pKa = 8.01 FTDD28 pKa = 3.5 NTIAGFVQQGTFDD41 pKa = 3.79 ADD43 pKa = 3.7 GYY45 pKa = 11.43 KK46 pKa = 10.45 RR47 pKa = 11.84 ITGDD51 pKa = 3.57 DD52 pKa = 3.91 YY53 pKa = 12.05 VAGDD57 pKa = 3.79 QNTVANGG64 pKa = 3.19
Molecular weight: 7.01 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.757
IPC2_protein 3.643
IPC_protein 3.579
Toseland 3.363
ProMoST 3.821
Dawson 3.617
Bjellqvist 3.783
Wikipedia 3.643
Rodwell 3.427
Grimsley 3.287
Solomon 3.579
Lehninger 3.541
Nozaki 3.77
DTASelect 4.05
Thurlkill 3.465
EMBOSS 3.643
Sillero 3.719
Patrickios 0.604
IPC_peptide 3.567
IPC2_peptide 3.681
IPC2.peptide.svr19 3.699
Protein with the highest isoelectric point:
>tr|X2CXM9|X2CXM9_9CAUD Uncharacterized protein OS=Lactobacillus phage phi jlb1 OX=1416334 GN=jlb1_010 PE=4 SV=1
MM1 pKa = 7.3 KK2 pKa = 10.39 RR3 pKa = 11.84 EE4 pKa = 4.13 LKK6 pKa = 10.24 SLRR9 pKa = 11.84 VGAGLTQAEE18 pKa = 4.32 LAKK21 pKa = 10.61 RR22 pKa = 11.84 LGVTNVTVSRR32 pKa = 11.84 WEE34 pKa = 3.89 RR35 pKa = 11.84 GEE37 pKa = 4.3 TIPKK41 pKa = 9.06 PKK43 pKa = 9.88 YY44 pKa = 8.24 IKK46 pKa = 10.98 AMAKK50 pKa = 9.91 LFNIKK55 pKa = 10.32 GQDD58 pKa = 2.8 IFLNLITTKK67 pKa = 10.32 AYY69 pKa = 9.84 KK70 pKa = 10.4 VVTKK74 pKa = 10.85
Molecular weight: 8.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.205
IPC2_protein 9.794
IPC_protein 9.97
Toseland 10.833
ProMoST 10.379
Dawson 10.906
Bjellqvist 10.496
Wikipedia 11.023
Rodwell 11.506
Grimsley 10.935
Solomon 10.95
Lehninger 10.95
Nozaki 10.789
DTASelect 10.496
Thurlkill 10.804
EMBOSS 11.213
Sillero 10.818
Patrickios 11.257
IPC_peptide 10.965
IPC2_peptide 8.916
IPC2.peptide.svr19 8.702
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
54
0
54
11378
43
1136
210.7
23.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.725 ± 0.934
0.536 ± 0.129
7.312 ± 0.381
5.968 ± 0.539
3.401 ± 0.174
6.46 ± 0.588
1.266 ± 0.191
6.056 ± 0.293
8.552 ± 0.441
8.666 ± 0.402
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.435 ± 0.329
5.994 ± 0.44
2.997 ± 0.201
3.929 ± 0.172
4.025 ± 0.359
6.266 ± 0.239
6.205 ± 0.44
6.715 ± 0.404
1.318 ± 0.172
4.175 ± 0.429
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here