Bifidobacterium bombi DSM 19703
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1454 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A080N3X3|A0A080N3X3_9BIFI Transcriptional regulator MarR family OS=Bifidobacterium bombi DSM 19703 OX=1341695 GN=BBOMB_1147 PE=4 SV=1
MM1 pKa = 7.13 AQDD4 pKa = 3.51 YY5 pKa = 10.08 DD6 pKa = 3.82 SPRR9 pKa = 11.84 NKK11 pKa = 10.84 DD12 pKa = 3.11 EE13 pKa = 5.41 DD14 pKa = 3.99 EE15 pKa = 4.81 EE16 pKa = 4.49 SLQEE20 pKa = 4.65 LGKK23 pKa = 10.64 SSQNEE28 pKa = 3.91 NTDD31 pKa = 3.05 IDD33 pKa = 4.22 DD34 pKa = 4.69 DD35 pKa = 4.47 EE36 pKa = 4.39 NAIAEE41 pKa = 4.35 DD42 pKa = 4.23 YY43 pKa = 9.11 EE44 pKa = 5.05 LPGADD49 pKa = 4.92 LSNEE53 pKa = 4.01 DD54 pKa = 3.61 SSVTVIPMQGDD65 pKa = 3.58 EE66 pKa = 5.57 FICSQCFLVKK76 pKa = 10.28 HH77 pKa = 6.44 RR78 pKa = 11.84 SQLAYY83 pKa = 9.19 TDD85 pKa = 4.37 EE86 pKa = 4.65 SGQPICKK93 pKa = 9.77 EE94 pKa = 4.0 CAAA97 pKa = 4.74
Molecular weight: 10.78 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.732
IPC_protein 3.694
Toseland 3.49
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.617
Rodwell 3.528
Grimsley 3.401
Solomon 3.668
Lehninger 3.617
Nozaki 3.808
DTASelect 3.999
Thurlkill 3.541
EMBOSS 3.617
Sillero 3.821
Patrickios 1.837
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A080N687|A0A080N687_9BIFI Probable potassium transport system protein kup OS=Bifidobacterium bombi DSM 19703 OX=1341695 GN=kup PE=3 SV=1
MM1 pKa = 7.46 GSIIKK6 pKa = 9.98 KK7 pKa = 8.35 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.39 RR11 pKa = 11.84 MSKK14 pKa = 9.63 KK15 pKa = 9.39 KK16 pKa = 9.21 HH17 pKa = 5.44 RR18 pKa = 11.84 KK19 pKa = 6.23 MLRR22 pKa = 11.84 KK23 pKa = 7.88 TRR25 pKa = 11.84 HH26 pKa = 3.78 QRR28 pKa = 11.84 KK29 pKa = 8.94
Molecular weight: 3.73 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.067
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.808
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.676
Grimsley 12.837
Solomon 13.29
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.398
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.038
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1454
0
1454
522551
29
3126
359.4
39.25
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.958 ± 0.061
1.013 ± 0.024
6.624 ± 0.058
5.51 ± 0.063
3.405 ± 0.041
8.382 ± 0.066
2.253 ± 0.027
5.044 ± 0.048
4.073 ± 0.054
8.793 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.627 ± 0.032
3.359 ± 0.042
4.544 ± 0.038
3.661 ± 0.04
6.287 ± 0.064
6.887 ± 0.053
5.695 ± 0.049
7.98 ± 0.059
1.298 ± 0.022
2.607 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here