Paremcibacter congregatus
Average proteome isoelectric point is 6.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3533 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2G4YRT2|A0A2G4YRT2_9PROT Uncharacterized protein OS=Paremcibacter congregatus OX=2043170 GN=CRD36_09960 PE=4 SV=1
MM1 pKa = 7.08 SQEE4 pKa = 4.55 DD5 pKa = 4.54 DD6 pKa = 3.4 IASAMAYY13 pKa = 8.07 VTDD16 pKa = 3.92 VKK18 pKa = 10.94 FEE20 pKa = 3.95 YY21 pKa = 10.81 AAMINTLYY29 pKa = 10.38 YY30 pKa = 10.46 LCEE33 pKa = 3.96 EE34 pKa = 4.74 ADD36 pKa = 4.2 SIGLPDD42 pKa = 3.62 VSLHH46 pKa = 5.97 LKK48 pKa = 9.88 IAIDD52 pKa = 3.6 EE53 pKa = 4.58 LKK55 pKa = 11.03 AQGPRR60 pKa = 11.84 DD61 pKa = 3.47 ASQDD65 pKa = 3.59 DD66 pKa = 4.09 EE67 pKa = 5.39 EE68 pKa = 4.91 ITSIWPSAPTEE79 pKa = 3.96
Molecular weight: 8.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.755
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.528
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.948
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.846
Patrickios 1.863
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.8
Protein with the highest isoelectric point:
>tr|A0A2G4YWF6|A0A2G4YWF6_9PROT Uncharacterized protein OS=Paremcibacter congregatus OX=2043170 GN=CRD36_01995 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.63 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 SATVGGRR28 pKa = 11.84 RR29 pKa = 11.84 VLSARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.97 GRR39 pKa = 11.84 KK40 pKa = 8.91 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3533
0
3533
1223468
21
4919
346.3
38.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.382 ± 0.04
0.914 ± 0.014
6.203 ± 0.031
5.896 ± 0.039
4.036 ± 0.029
7.864 ± 0.079
2.204 ± 0.021
6.243 ± 0.035
4.938 ± 0.034
9.794 ± 0.052
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.753 ± 0.024
3.726 ± 0.029
4.348 ± 0.027
3.656 ± 0.022
5.374 ± 0.034
5.983 ± 0.035
5.722 ± 0.036
6.742 ± 0.032
1.176 ± 0.015
3.048 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here