Leptonychotes weddellii papillomavirus 4
Average proteome isoelectric point is 5.98
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2I8B2R1|A0A2I8B2R1_9PAPI Minor capsid protein L2 OS=Leptonychotes weddellii papillomavirus 4 OX=2077305 GN=L2 PE=3 SV=1
MM1 pKa = 7.4 IGKK4 pKa = 9.27 EE5 pKa = 3.72 PTLRR9 pKa = 11.84 DD10 pKa = 3.17 IVLEE14 pKa = 4.03 EE15 pKa = 4.54 TPCPADD21 pKa = 3.91 LYY23 pKa = 9.64 CNEE26 pKa = 4.78 RR27 pKa = 11.84 IEE29 pKa = 4.33 EE30 pKa = 4.23 AEE32 pKa = 4.06 QNISEE37 pKa = 4.37 GNHH40 pKa = 6.34 DD41 pKa = 3.96 AFKK44 pKa = 11.22 VLVTCGHH51 pKa = 6.96 CDD53 pKa = 2.98 RR54 pKa = 11.84 PVRR57 pKa = 11.84 LVILASRR64 pKa = 11.84 DD65 pKa = 3.51 QVRR68 pKa = 11.84 VLHH71 pKa = 6.51 RR72 pKa = 11.84 LLLEE76 pKa = 4.3 GLCFVCPYY84 pKa = 9.85 CCRR87 pKa = 11.84 EE88 pKa = 3.37 RR89 pKa = 11.84 DD90 pKa = 3.75 YY91 pKa = 11.94 NN92 pKa = 3.67
Molecular weight: 10.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.872
IPC2_protein 5.118
IPC_protein 4.978
Toseland 4.914
ProMoST 5.118
Dawson 4.965
Bjellqvist 5.092
Wikipedia 4.825
Rodwell 4.889
Grimsley 4.838
Solomon 4.965
Lehninger 4.927
Nozaki 5.092
DTASelect 5.207
Thurlkill 4.94
EMBOSS 4.876
Sillero 5.169
Patrickios 3.007
IPC_peptide 4.978
IPC2_peptide 5.169
IPC2.peptide.svr19 5.121
Protein with the highest isoelectric point:
>tr|A0A2I8B2R5|A0A2I8B2R5_9PAPI Regulatory protein E2 OS=Leptonychotes weddellii papillomavirus 4 OX=2077305 GN=E2 PE=3 SV=1
MM1 pKa = 6.97 EE2 pKa = 4.63 TLSRR6 pKa = 11.84 RR7 pKa = 11.84 LDD9 pKa = 3.59 ALQEE13 pKa = 4.08 EE14 pKa = 5.04 LLNLYY19 pKa = 8.49 EE20 pKa = 4.81 KK21 pKa = 10.77 SSEE24 pKa = 4.02 QLEE27 pKa = 4.33 HH28 pKa = 7.28 QILHH32 pKa = 5.49 WQLLRR37 pKa = 11.84 QEE39 pKa = 4.53 NVLLHH44 pKa = 5.05 YY45 pKa = 10.25 AKK47 pKa = 10.5 KK48 pKa = 10.51 RR49 pKa = 11.84 GVCRR53 pKa = 11.84 LGYY56 pKa = 9.47 QAVPAAVVTQEE67 pKa = 4.03 KK68 pKa = 10.09 AKK70 pKa = 10.43 QAIEE74 pKa = 4.07 MEE76 pKa = 4.39 LVLTSLSKK84 pKa = 10.75 SAFSKK89 pKa = 10.61 EE90 pKa = 3.76 PWTLTDD96 pKa = 3.13 TSRR99 pKa = 11.84 DD100 pKa = 3.32 MWCAEE105 pKa = 3.74 PRR107 pKa = 11.84 HH108 pKa = 5.94 CFKK111 pKa = 11.02 KK112 pKa = 10.54 GGSQVEE118 pKa = 4.75 VVFGACPSDD127 pKa = 3.76 SMLYY131 pKa = 7.99 TCWGSVYY138 pKa = 10.69 YY139 pKa = 10.47 QDD141 pKa = 6.13 ADD143 pKa = 4.21 DD144 pKa = 4.2 VWHH147 pKa = 6.37 KK148 pKa = 9.77 TSSTVCHH155 pKa = 6.32 AGVFFMEE162 pKa = 5.53 GSQAKK167 pKa = 9.92 YY168 pKa = 9.97 YY169 pKa = 11.14 VDD171 pKa = 4.9 FSSEE175 pKa = 3.58 AARR178 pKa = 11.84 YY179 pKa = 7.54 GASDD183 pKa = 3.03 WTVIHH188 pKa = 5.95 KK189 pKa = 7.99 TAVYY193 pKa = 10.3 SSVAVTSSEE202 pKa = 4.14 CGEE205 pKa = 4.02 AAAGSDD211 pKa = 3.33 QRR213 pKa = 11.84 RR214 pKa = 11.84 AGVVRR219 pKa = 11.84 RR220 pKa = 11.84 RR221 pKa = 11.84 GRR223 pKa = 11.84 PPKK226 pKa = 10.23 KK227 pKa = 9.53 YY228 pKa = 10.38 GRR230 pKa = 11.84 AASPLPTEE238 pKa = 4.23 TQEE241 pKa = 4.23 TVTDD245 pKa = 4.27 SVAPGYY251 pKa = 9.5 PGLPGPPGLPGPPGPKK267 pKa = 9.44 RR268 pKa = 11.84 PCTGWGGPTTSGASPGACEE287 pKa = 4.18 PSITAASATDD297 pKa = 3.69 PTGSRR302 pKa = 11.84 LPPLTVSGQLGRR314 pKa = 11.84 GQGEE318 pKa = 4.31 PEE320 pKa = 3.88 TRR322 pKa = 11.84 STFWQQEE329 pKa = 4.31 EE330 pKa = 5.14 GEE332 pKa = 4.45 HH333 pKa = 5.57 TRR335 pKa = 11.84 SEE337 pKa = 4.44 PEE339 pKa = 3.67 STPPRR344 pKa = 11.84 EE345 pKa = 4.59 GYY347 pKa = 10.93 ANPRR351 pKa = 11.84 PDD353 pKa = 3.72 SSTPDD358 pKa = 3.27 PVVARR363 pKa = 11.84 RR364 pKa = 11.84 RR365 pKa = 11.84 SPGLGRR371 pKa = 11.84 SPVWWPPCALPGEE384 pKa = 4.46 IPPPVARR391 pKa = 11.84 HH392 pKa = 5.17 NSEE395 pKa = 3.96 SPRR398 pKa = 11.84 CEE400 pKa = 3.88 DD401 pKa = 3.24 SPPVLEE407 pKa = 4.85 EE408 pKa = 4.06 EE409 pKa = 4.45 ACEE412 pKa = 4.09 GHH414 pKa = 6.43 SPTSSKK420 pKa = 10.48 RR421 pKa = 11.84 KK422 pKa = 9.29 QPEE425 pKa = 3.42 QTKK428 pKa = 9.61 RR429 pKa = 11.84 SRR431 pKa = 11.84 RR432 pKa = 11.84 PRR434 pKa = 11.84 AVLQPAGSSPVVLLKK449 pKa = 10.92 GGCNQLKK456 pKa = 9.65 CLRR459 pKa = 11.84 YY460 pKa = 9.63 RR461 pKa = 11.84 LRR463 pKa = 11.84 TKK465 pKa = 9.39 HH466 pKa = 6.05 KK467 pKa = 8.2 KK468 pKa = 7.27 WVRR471 pKa = 11.84 LVSTTFFWVGNDD483 pKa = 2.98 SVNRR487 pKa = 11.84 LGRR490 pKa = 11.84 ARR492 pKa = 11.84 ILVTFRR498 pKa = 11.84 SHH500 pKa = 6.87 AACEE504 pKa = 3.99 EE505 pKa = 4.1 FLNSGNVPQGVEE517 pKa = 3.3 AALIHH522 pKa = 6.12 GLL524 pKa = 3.68
Molecular weight: 57.32 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.559
IPC2_protein 7.834
IPC_protein 7.644
Toseland 7.22
ProMoST 8.244
Dawson 8.361
Bjellqvist 8.799
Wikipedia 8.273
Rodwell 8.39
Grimsley 7.249
Solomon 8.419
Lehninger 8.434
Nozaki 9.048
DTASelect 8.478
Thurlkill 8.536
EMBOSS 8.565
Sillero 8.887
Patrickios 4.457
IPC_peptide 8.419
IPC2_peptide 7.878
IPC2.peptide.svr19 7.927
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2403
92
628
400.5
44.34
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.825 ± 0.546
2.622 ± 0.702
6.117 ± 0.78
6.409 ± 0.506
3.829 ± 0.45
7.74 ± 1.001
2.164 ± 0.209
2.705 ± 0.36
4.12 ± 0.634
8.365 ± 0.581
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.415 ± 0.381
3.038 ± 0.589
6.991 ± 1.037
3.246 ± 0.517
6.866 ± 0.516
8.198 ± 0.588
6.866 ± 0.769
7.532 ± 0.649
1.498 ± 0.393
3.454 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here