Antrodiella citrinella
Average proteome isoelectric point is 6.23
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 9798 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4S4MPP3|A0A4S4MPP3_9APHY HIG1 domain-containing protein OS=Antrodiella citrinella OX=2447956 GN=EUX98_g6970 PE=4 SV=1
MM1 pKa = 7.21 PTASTDD7 pKa = 3.82 FTLEE11 pKa = 4.06 LPAMDD16 pKa = 5.15 SDD18 pKa = 6.14 LMPEE22 pKa = 3.96 WLYY25 pKa = 11.24 EE26 pKa = 4.19 PGPEE30 pKa = 4.86 IPLTQDD36 pKa = 3.0 DD37 pKa = 3.83 VRR39 pKa = 11.84 RR40 pKa = 11.84 LYY42 pKa = 11.02 RR43 pKa = 11.84 NSTCTTTEE51 pKa = 3.96 DD52 pKa = 4.2 SLSLGYY58 pKa = 10.79 GSIRR62 pKa = 11.84 APSPAEE68 pKa = 3.95 LQSLWDD74 pKa = 4.28 DD75 pKa = 4.07 SEE77 pKa = 5.05 SDD79 pKa = 3.57 SGSDD83 pKa = 4.96 DD84 pKa = 4.0 EE85 pKa = 5.8 PFDD88 pKa = 6.36 DD89 pKa = 5.99 EE90 pKa = 7.33 DD91 pKa = 5.94 DD92 pKa = 4.75 DD93 pKa = 5.71 EE94 pKa = 4.62 NLCVSDD100 pKa = 5.23 ADD102 pKa = 4.02 TDD104 pKa = 4.06 STSATLHH111 pKa = 6.54 DD112 pKa = 5.01 APVDD116 pKa = 4.02 PADD119 pKa = 4.27 HH120 pKa = 6.7 APSTSALKK128 pKa = 10.22 QPQLISPPSPPSPRR142 pKa = 11.84 RR143 pKa = 11.84 HH144 pKa = 6.36 PDD146 pKa = 3.14 DD147 pKa = 4.29 LLRR150 pKa = 11.84 LYY152 pKa = 10.04 AYY154 pKa = 10.7 SSADD158 pKa = 3.48 PDD160 pKa = 4.66 LATCLHH166 pKa = 6.9 GIDD169 pKa = 4.78 EE170 pKa = 5.14 ALLCNPFSVYY180 pKa = 11.26 ADD182 pKa = 3.36 VCYY185 pKa = 10.48 NAAVKK190 pKa = 10.47 RR191 pKa = 11.84 EE192 pKa = 4.01 PQNGGVSRR200 pKa = 11.84 GSNEE204 pKa = 3.79 LEE206 pKa = 3.7 RR207 pKa = 11.84 VTEE210 pKa = 3.87 EE211 pKa = 5.06 DD212 pKa = 3.21 EE213 pKa = 4.39 TEE215 pKa = 3.96 EE216 pKa = 4.45 AEE218 pKa = 4.37 SEE220 pKa = 4.14 EE221 pKa = 5.28 SEE223 pKa = 5.71 DD224 pKa = 3.29 EE225 pKa = 4.33 DD226 pKa = 4.75 EE227 pKa = 5.03 PVLHH231 pKa = 6.97 SPIQTGVVASNWTSGVYY248 pKa = 9.82 VGNAPSEE255 pKa = 4.18 ALSALALPAPYY266 pKa = 9.64 HH267 pKa = 5.81 ATMDD271 pKa = 3.88 SKK273 pKa = 10.87 PGCFVSPVSTNN284 pKa = 2.84
Molecular weight: 30.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.693
IPC2_protein 3.757
IPC_protein 3.77
Toseland 3.554
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.465
Solomon 3.757
Lehninger 3.706
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.897
Patrickios 1.265
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.795
Protein with the highest isoelectric point:
>tr|A0A4S4MVL2|A0A4S4MVL2_9APHY Signal recognition particle receptor subunit beta OS=Antrodiella citrinella OX=2447956 GN=EUX98_g3829 PE=3 SV=1
MM1 pKa = 6.8 THH3 pKa = 6.6 DD4 pKa = 5.65 AIWFSRR10 pKa = 11.84 PRR12 pKa = 11.84 KK13 pKa = 7.98 YY14 pKa = 11.02 GKK16 pKa = 10.34 GSRR19 pKa = 11.84 QCRR22 pKa = 11.84 LCAHH26 pKa = 5.84 QAGLIRR32 pKa = 11.84 KK33 pKa = 9.09 YY34 pKa = 10.91 GIDD37 pKa = 3.51 LCRR40 pKa = 11.84 QCFRR44 pKa = 11.84 EE45 pKa = 4.02 KK46 pKa = 10.43 SAAIGFTKK54 pKa = 10.0 TRR56 pKa = 3.53
Molecular weight: 6.52 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.351
IPC2_protein 9.604
IPC_protein 10.131
Toseland 10.584
ProMoST 10.511
Dawson 10.687
Bjellqvist 10.394
Wikipedia 10.862
Rodwell 11.023
Grimsley 10.73
Solomon 10.774
Lehninger 10.76
Nozaki 10.628
DTASelect 10.365
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.628
Patrickios 10.804
IPC_peptide 10.789
IPC2_peptide 9.677
IPC2.peptide.svr19 8.599
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
9798
0
9798
4715391
28
4919
481.3
53.14
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.829 ± 0.02
1.08 ± 0.008
6.015 ± 0.019
6.098 ± 0.024
3.703 ± 0.015
6.339 ± 0.02
2.536 ± 0.011
4.761 ± 0.017
4.703 ± 0.021
9.026 ± 0.027
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.155 ± 0.008
3.378 ± 0.014
6.413 ± 0.028
3.75 ± 0.016
5.972 ± 0.021
8.341 ± 0.029
6.118 ± 0.015
6.671 ± 0.019
1.373 ± 0.007
2.678 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here