Acetobacter peroxydans
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2457 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Y3TW46|A0A4Y3TW46_9PROT Transcriptional regulator OS=Acetobacter peroxydans OX=104098 GN=APE01nite_24130 PE=4 SV=1
MM1 pKa = 7.4 SVFNALSTAVSGINAQSTAFTNLSNNIANSQTVGYY36 pKa = 9.73 KK37 pKa = 9.3 ATTTAFQDD45 pKa = 4.29 FVAGAGGNSASADD58 pKa = 3.19 ISDD61 pKa = 3.73 SVAAVTVQHH70 pKa = 6.61 VDD72 pKa = 3.23 NQGTSSTSTDD82 pKa = 3.16 ALATSISGNGLFVVSKK98 pKa = 8.93 GTTSGGQTYY107 pKa = 7.16 YY108 pKa = 10.29 TRR110 pKa = 11.84 NGEE113 pKa = 4.17 VYY115 pKa = 9.79 EE116 pKa = 4.24 NKK118 pKa = 10.34 AGYY121 pKa = 9.43 LVNTSGYY128 pKa = 8.12 YY129 pKa = 10.29 LEE131 pKa = 6.37 GYY133 pKa = 8.6 MVDD136 pKa = 4.0 PSTGTLGTTLTQLNVSNVTFKK157 pKa = 9.98 PTEE160 pKa = 4.22 TTTLTSTSTVGTATGTNQTYY180 pKa = 7.15 TTAPTTVYY188 pKa = 10.5 DD189 pKa = 3.97 SSGTAHH195 pKa = 6.89 QIGLVWTQSTSDD207 pKa = 3.26 TSKK210 pKa = 8.4 WTVQAYY216 pKa = 9.86 DD217 pKa = 3.69 ADD219 pKa = 4.15 GGGAISDD226 pKa = 4.52 ASPSPYY232 pKa = 9.72 IVTFDD237 pKa = 3.54 SSTGAMTSVTDD248 pKa = 3.33 ATGAVVANTTGTAAEE263 pKa = 4.13 IPITATYY270 pKa = 10.49 NGVAQTINLDD280 pKa = 3.56 LGSIGSTSGTVVATAKK296 pKa = 10.51 SGTPSTSQGTSLVVSGGSLAMDD318 pKa = 3.67 SGGGAVNIGAQTGTNDD334 pKa = 3.39 TYY336 pKa = 9.62 TTTPLKK342 pKa = 10.72 LSDD345 pKa = 3.65 GSYY348 pKa = 11.21 VSAVWTKK355 pKa = 11.14 NPTTAANVNSEE366 pKa = 4.23 QTWTVKK372 pKa = 10.66 LVDD375 pKa = 4.81 PYY377 pKa = 11.78 SSATSDD383 pKa = 3.25 STTAQVTFKK392 pKa = 11.14 ADD394 pKa = 3.53 GTIDD398 pKa = 3.95 TINGSSTGSISFTDD412 pKa = 3.39 SSGTTQTLNLTTSSVKK428 pKa = 10.53 LGSSSPYY435 pKa = 9.5 TDD437 pKa = 3.19 TTAVTSDD444 pKa = 3.96 SIATGTYY451 pKa = 8.67 EE452 pKa = 4.02 GAEE455 pKa = 4.1 ITSDD459 pKa = 3.25 GSVMAKK465 pKa = 10.45 FSTGTQLIGKK475 pKa = 8.56 VALANFANVNGLEE488 pKa = 4.24 GVDD491 pKa = 3.54 GQAYY495 pKa = 9.72 VATTNSGNAQIGVSGQNGTGTLSVGYY521 pKa = 9.77 VEE523 pKa = 6.33 SSTTDD528 pKa = 3.22 LTKK531 pKa = 10.76 DD532 pKa = 3.4 LSSLIVAQQAYY543 pKa = 5.96 TANTKK548 pKa = 9.92 VVTSANTMLQATIAMIQQ565 pKa = 2.99
Molecular weight: 56.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.798
IPC2_protein 3.935
IPC_protein 3.961
Toseland 3.732
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.91
Rodwell 3.783
Grimsley 3.63
Solomon 3.961
Lehninger 3.91
Nozaki 4.075
DTASelect 4.355
Thurlkill 3.783
EMBOSS 3.923
Sillero 4.088
Patrickios 0.68
IPC_peptide 3.948
IPC2_peptide 4.062
IPC2.peptide.svr19 3.957
Protein with the highest isoelectric point:
>tr|A0A4Y3TVP7|A0A4Y3TVP7_9PROT Uncharacterized protein OS=Acetobacter peroxydans OX=104098 GN=APE01nite_22830 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.4 RR3 pKa = 11.84 TYY5 pKa = 10.2 QPSRR9 pKa = 11.84 LVRR12 pKa = 11.84 KK13 pKa = 8.99 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.7 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MATVGGRR28 pKa = 11.84 KK29 pKa = 9.32 VIANRR34 pKa = 11.84 RR35 pKa = 11.84 SKK37 pKa = 10.46 GRR39 pKa = 11.84 KK40 pKa = 8.72 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.448
IPC2_protein 11.155
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.384
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.106
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2457
0
2457
798204
43
1710
324.9
35.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.45 ± 0.072
1.096 ± 0.017
5.283 ± 0.034
5.349 ± 0.052
3.394 ± 0.029
8.37 ± 0.045
2.353 ± 0.022
4.539 ± 0.032
2.653 ± 0.042
10.635 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.596 ± 0.021
2.534 ± 0.029
5.573 ± 0.038
3.692 ± 0.031
7.226 ± 0.046
5.597 ± 0.038
5.744 ± 0.039
7.404 ± 0.043
1.358 ± 0.02
2.152 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here