Mycobacterium phage Barnyard

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Barnyardvirus; Mycobacterium virus Barnyard

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q856A2|Q856A2_9CAUD Uncharacterized protein OS=Mycobacterium phage Barnyard OX=205880 GN=70 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.28QEE5 pKa = 3.99TLLITVNCDD14 pKa = 2.95VKK16 pKa = 11.4GCANSIACGSNWSYY30 pKa = 11.18PEE32 pKa = 3.73EE33 pKa = 4.38QGWHH37 pKa = 6.61LDD39 pKa = 3.52VPVDD43 pKa = 3.73TRR45 pKa = 11.84HH46 pKa = 6.11MDD48 pKa = 4.05LCPEE52 pKa = 4.5CYY54 pKa = 10.41GDD56 pKa = 5.55LKK58 pKa = 11.23AFTDD62 pKa = 4.33GDD64 pKa = 4.12PDD66 pKa = 4.45DD67 pKa = 5.08EE68 pKa = 4.23QDD70 pKa = 3.45IMNLRR75 pKa = 11.84DD76 pKa = 3.6VLAGEE81 pKa = 4.16QPRR84 pKa = 11.84PEE86 pKa = 4.24DD87 pKa = 3.77AKK89 pKa = 11.41NDD91 pKa = 3.61

Molecular weight: 10.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q856G5|Q856G5_9CAUD Uncharacterized protein OS=Mycobacterium phage Barnyard OX=205880 GN=7 PE=4 SV=1
MM1 pKa = 7.56ARR3 pKa = 11.84RR4 pKa = 11.84ARR6 pKa = 11.84RR7 pKa = 11.84KK8 pKa = 9.38FKK10 pKa = 10.9ASLRR14 pKa = 11.84MIGGRR19 pKa = 11.84TVLITPGLRR28 pKa = 11.84RR29 pKa = 11.84VRR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84SKK35 pKa = 9.93RR36 pKa = 11.84ASSPTVDD43 pKa = 5.3RR44 pKa = 11.84MVQPP48 pKa = 4.41

Molecular weight: 5.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

22303

44

2047

204.6

22.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.81 ± 0.334

0.968 ± 0.124

6.353 ± 0.266

6.125 ± 0.27

3.399 ± 0.179

8.474 ± 0.484

2.184 ± 0.157

4.851 ± 0.18

5.165 ± 0.315

7.255 ± 0.248

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.524 ± 0.131

4.21 ± 0.165

5.753 ± 0.325

4.08 ± 0.254

6.497 ± 0.335

5.183 ± 0.129

5.883 ± 0.208

6.6 ± 0.165

1.982 ± 0.1

2.704 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski