Amino acid dipepetide frequency for Mycobacterium phage Barnyard

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.178AlaAla: 10.178 ± 0.991
0.717AlaCys: 0.717 ± 0.194
6.188AlaAsp: 6.188 ± 0.512
7.264AlaGlu: 7.264 ± 0.662
3.228AlaPhe: 3.228 ± 0.466
8.161AlaGly: 8.161 ± 0.985
1.794AlaHis: 1.794 ± 0.308
4.932AlaIle: 4.932 ± 0.559
4.574AlaLys: 4.574 ± 0.374
8.206AlaLeu: 8.206 ± 0.637
2.376AlaMet: 2.376 ± 0.312
4.618AlaAsn: 4.618 ± 0.467
3.856AlaPro: 3.856 ± 0.454
4.215AlaGln: 4.215 ± 0.506
6.098AlaArg: 6.098 ± 0.558
5.201AlaSer: 5.201 ± 0.482
5.426AlaThr: 5.426 ± 0.451
7.085AlaVal: 7.085 ± 0.538
1.704AlaTrp: 1.704 ± 0.25
2.242AlaTyr: 2.242 ± 0.337
0.0AlaXaa: 0.0 ± 0.0
Cys
1.121CysAla: 1.121 ± 0.261
0.179CysCys: 0.179 ± 0.1
0.717CysAsp: 0.717 ± 0.211
0.359CysGlu: 0.359 ± 0.12
0.179CysPhe: 0.179 ± 0.084
0.897CysGly: 0.897 ± 0.23
0.404CysHis: 0.404 ± 0.162
0.538CysIle: 0.538 ± 0.151
0.179CysLys: 0.179 ± 0.098
0.628CysLeu: 0.628 ± 0.212
0.09CysMet: 0.09 ± 0.063
0.314CysAsn: 0.314 ± 0.132
0.807CysPro: 0.807 ± 0.202
0.269CysGln: 0.269 ± 0.114
0.717CysArg: 0.717 ± 0.169
0.717CysSer: 0.717 ± 0.198
0.717CysThr: 0.717 ± 0.208
0.493CysVal: 0.493 ± 0.203
0.179CysTrp: 0.179 ± 0.086
0.179CysTyr: 0.179 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
6.143AspAla: 6.143 ± 0.554
0.314AspCys: 0.314 ± 0.12
4.439AspAsp: 4.439 ± 0.599
4.843AspGlu: 4.843 ± 0.521
2.332AspPhe: 2.332 ± 0.466
4.349AspGly: 4.349 ± 0.545
1.569AspHis: 1.569 ± 0.298
2.825AspIle: 2.825 ± 0.343
3.094AspLys: 3.094 ± 0.492
4.753AspLeu: 4.753 ± 0.576
2.063AspMet: 2.063 ± 0.258
1.883AspAsn: 1.883 ± 0.293
4.977AspPro: 4.977 ± 0.499
2.915AspGln: 2.915 ± 0.379
3.318AspArg: 3.318 ± 0.404
3.408AspSer: 3.408 ± 0.396
3.228AspThr: 3.228 ± 0.368
3.946AspVal: 3.946 ± 0.385
1.345AspTrp: 1.345 ± 0.264
1.794AspTyr: 1.794 ± 0.333
0.0AspXaa: 0.0 ± 0.0
Glu
6.188GluAla: 6.188 ± 0.447
0.762GluCys: 0.762 ± 0.202
3.094GluAsp: 3.094 ± 0.345
2.78GluGlu: 2.78 ± 0.347
2.959GluPhe: 2.959 ± 0.405
4.753GluGly: 4.753 ± 0.467
1.031GluHis: 1.031 ± 0.241
3.587GluIle: 3.587 ± 0.444
2.646GluLys: 2.646 ± 0.394
4.977GluLeu: 4.977 ± 0.461
1.3GluMet: 1.3 ± 0.29
2.78GluAsn: 2.78 ± 0.295
3.632GluPro: 3.632 ± 0.611
2.959GluGln: 2.959 ± 0.4
3.766GluArg: 3.766 ± 0.477
3.318GluSer: 3.318 ± 0.422
3.766GluThr: 3.766 ± 0.49
3.722GluVal: 3.722 ± 0.423
1.211GluTrp: 1.211 ± 0.228
2.018GluTyr: 2.018 ± 0.38
0.0GluXaa: 0.0 ± 0.0
Phe
3.946PheAla: 3.946 ± 0.531
0.583PheCys: 0.583 ± 0.193
2.825PheAsp: 2.825 ± 0.333
2.197PheGlu: 2.197 ± 0.329
0.628PhePhe: 0.628 ± 0.143
3.184PheGly: 3.184 ± 0.434
0.852PheHis: 0.852 ± 0.179
1.614PheIle: 1.614 ± 0.206
1.749PheLys: 1.749 ± 0.225
2.152PheLeu: 2.152 ± 0.361
0.538PheMet: 0.538 ± 0.157
1.928PheAsn: 1.928 ± 0.301
1.031PhePro: 1.031 ± 0.201
1.255PheGln: 1.255 ± 0.255
2.601PheArg: 2.601 ± 0.309
1.39PheSer: 1.39 ± 0.193
2.018PheThr: 2.018 ± 0.265
2.063PheVal: 2.063 ± 0.297
0.493PheTrp: 0.493 ± 0.132
0.942PheTyr: 0.942 ± 0.248
0.0PheXaa: 0.0 ± 0.0
Gly
6.905GlyAla: 6.905 ± 1.001
0.942GlyCys: 0.942 ± 0.254
4.932GlyAsp: 4.932 ± 0.491
4.663GlyGlu: 4.663 ± 0.412
2.735GlyPhe: 2.735 ± 0.379
9.192GlyGly: 9.192 ± 1.343
1.704GlyHis: 1.704 ± 0.339
4.125GlyIle: 4.125 ± 0.432
5.201GlyLys: 5.201 ± 0.502
5.426GlyLeu: 5.426 ± 0.67
2.242GlyMet: 2.242 ± 0.296
4.215GlyAsn: 4.215 ± 0.641
3.632GlyPro: 3.632 ± 0.429
3.856GlyGln: 3.856 ± 0.548
4.887GlyArg: 4.887 ± 0.561
4.574GlySer: 4.574 ± 0.628
5.829GlyThr: 5.829 ± 0.786
5.336GlyVal: 5.336 ± 0.483
1.749GlyTrp: 1.749 ± 0.318
2.601GlyTyr: 2.601 ± 0.295
0.0GlyXaa: 0.0 ± 0.0
His
2.018HisAla: 2.018 ± 0.384
0.493HisCys: 0.493 ± 0.164
1.614HisAsp: 1.614 ± 0.297
1.48HisGlu: 1.48 ± 0.302
0.314HisPhe: 0.314 ± 0.116
1.838HisGly: 1.838 ± 0.329
0.448HisHis: 0.448 ± 0.159
0.942HisIle: 0.942 ± 0.178
1.211HisLys: 1.211 ± 0.292
1.39HisLeu: 1.39 ± 0.218
0.538HisMet: 0.538 ± 0.147
0.628HisAsn: 0.628 ± 0.171
1.345HisPro: 1.345 ± 0.286
0.448HisGln: 0.448 ± 0.142
2.018HisArg: 2.018 ± 0.295
1.166HisSer: 1.166 ± 0.275
1.031HisThr: 1.031 ± 0.22
1.614HisVal: 1.614 ± 0.348
0.448HisTrp: 0.448 ± 0.155
0.852HisTyr: 0.852 ± 0.223
0.0HisXaa: 0.0 ± 0.0
Ile
5.426IleAla: 5.426 ± 0.454
0.224IleCys: 0.224 ± 0.108
3.811IleAsp: 3.811 ± 0.351
3.049IleGlu: 3.049 ± 0.487
1.345IlePhe: 1.345 ± 0.198
3.766IleGly: 3.766 ± 0.406
0.852IleHis: 0.852 ± 0.201
1.928IleIle: 1.928 ± 0.336
2.421IleLys: 2.421 ± 0.396
3.049IleLeu: 3.049 ± 0.355
1.345IleMet: 1.345 ± 0.249
2.018IleAsn: 2.018 ± 0.337
3.856IlePro: 3.856 ± 0.401
1.838IleGln: 1.838 ± 0.341
3.766IleArg: 3.766 ± 0.413
2.107IleSer: 2.107 ± 0.3
2.78IleThr: 2.78 ± 0.346
2.87IleVal: 2.87 ± 0.34
0.673IleTrp: 0.673 ± 0.197
1.39IleTyr: 1.39 ± 0.257
0.0IleXaa: 0.0 ± 0.0
Lys
5.784LysAla: 5.784 ± 0.648
0.224LysCys: 0.224 ± 0.098
2.646LysAsp: 2.646 ± 0.379
2.376LysGlu: 2.376 ± 0.307
1.838LysPhe: 1.838 ± 0.359
3.811LysGly: 3.811 ± 0.375
1.166LysHis: 1.166 ± 0.237
2.376LysIle: 2.376 ± 0.311
3.811LysLys: 3.811 ± 0.684
4.349LysLeu: 4.349 ± 0.475
1.3LysMet: 1.3 ± 0.191
1.3LysAsn: 1.3 ± 0.192
3.318LysPro: 3.318 ± 0.472
2.332LysGln: 2.332 ± 0.349
4.439LysArg: 4.439 ± 0.716
2.421LysSer: 2.421 ± 0.294
2.87LysThr: 2.87 ± 0.32
3.049LysVal: 3.049 ± 0.347
1.031LysTrp: 1.031 ± 0.225
1.211LysTyr: 1.211 ± 0.29
0.0LysXaa: 0.0 ± 0.0
Leu
7.174LeuAla: 7.174 ± 0.763
0.807LeuCys: 0.807 ± 0.214
4.618LeuAsp: 4.618 ± 0.432
3.946LeuGlu: 3.946 ± 0.475
2.018LeuPhe: 2.018 ± 0.288
6.681LeuGly: 6.681 ± 0.614
1.838LeuHis: 1.838 ± 0.278
4.036LeuIle: 4.036 ± 0.471
3.184LeuLys: 3.184 ± 0.324
5.515LeuLeu: 5.515 ± 0.523
2.287LeuMet: 2.287 ± 0.345
3.004LeuAsn: 3.004 ± 0.362
4.574LeuPro: 4.574 ± 0.417
2.78LeuGln: 2.78 ± 0.478
4.932LeuArg: 4.932 ± 0.453
3.542LeuSer: 3.542 ± 0.376
3.991LeuThr: 3.991 ± 0.407
5.246LeuVal: 5.246 ± 0.526
1.121LeuTrp: 1.121 ± 0.226
1.255LeuTyr: 1.255 ± 0.276
0.0LeuXaa: 0.0 ± 0.0
Met
2.735MetAla: 2.735 ± 0.321
0.269MetCys: 0.269 ± 0.118
1.435MetAsp: 1.435 ± 0.249
1.3MetGlu: 1.3 ± 0.246
0.942MetPhe: 0.942 ± 0.211
1.928MetGly: 1.928 ± 0.284
0.493MetHis: 0.493 ± 0.14
1.345MetIle: 1.345 ± 0.255
0.986MetLys: 0.986 ± 0.189
1.749MetLeu: 1.749 ± 0.233
0.583MetMet: 0.583 ± 0.174
1.031MetAsn: 1.031 ± 0.207
1.883MetPro: 1.883 ± 0.263
1.121MetGln: 1.121 ± 0.277
1.569MetArg: 1.569 ± 0.247
1.614MetSer: 1.614 ± 0.262
1.838MetThr: 1.838 ± 0.296
1.345MetVal: 1.345 ± 0.242
0.404MetTrp: 0.404 ± 0.126
0.673MetTyr: 0.673 ± 0.19
0.0MetXaa: 0.0 ± 0.0
Asn
4.663AsnAla: 4.663 ± 0.543
0.359AsnCys: 0.359 ± 0.14
2.421AsnAsp: 2.421 ± 0.24
2.466AsnGlu: 2.466 ± 0.304
1.255AsnPhe: 1.255 ± 0.267
3.991AsnGly: 3.991 ± 0.742
0.807AsnHis: 0.807 ± 0.212
1.704AsnIle: 1.704 ± 0.28
2.018AsnLys: 2.018 ± 0.301
3.318AsnLeu: 3.318 ± 0.372
0.673AsnMet: 0.673 ± 0.156
1.569AsnAsn: 1.569 ± 0.298
3.139AsnPro: 3.139 ± 0.498
1.121AsnGln: 1.121 ± 0.259
2.825AsnArg: 2.825 ± 0.25
1.659AsnSer: 1.659 ± 0.238
2.466AsnThr: 2.466 ± 0.409
3.049AsnVal: 3.049 ± 0.321
1.525AsnTrp: 1.525 ± 0.206
1.076AsnTyr: 1.076 ± 0.239
0.0AsnXaa: 0.0 ± 0.0
Pro
4.932ProAla: 4.932 ± 0.63
0.314ProCys: 0.314 ± 0.124
4.843ProAsp: 4.843 ± 0.671
4.843ProGlu: 4.843 ± 0.734
1.794ProPhe: 1.794 ± 0.266
4.484ProGly: 4.484 ± 0.529
1.749ProHis: 1.749 ± 0.313
2.735ProIle: 2.735 ± 0.324
2.69ProLys: 2.69 ± 0.373
3.228ProLeu: 3.228 ± 0.348
1.345ProMet: 1.345 ± 0.214
2.466ProAsn: 2.466 ± 0.312
3.901ProPro: 3.901 ± 0.664
1.838ProGln: 1.838 ± 0.294
2.78ProArg: 2.78 ± 0.425
3.273ProSer: 3.273 ± 0.52
3.318ProThr: 3.318 ± 0.411
4.125ProVal: 4.125 ± 0.515
1.166ProTrp: 1.166 ± 0.22
1.704ProTyr: 1.704 ± 0.333
0.0ProXaa: 0.0 ± 0.0
Gln
4.484GlnAla: 4.484 ± 0.537
0.224GlnCys: 0.224 ± 0.103
1.749GlnAsp: 1.749 ± 0.255
1.794GlnGlu: 1.794 ± 0.288
1.794GlnPhe: 1.794 ± 0.393
2.78GlnGly: 2.78 ± 0.456
0.807GlnHis: 0.807 ± 0.181
2.466GlnIle: 2.466 ± 0.297
2.107GlnLys: 2.107 ± 0.307
3.677GlnLeu: 3.677 ± 0.417
1.659GlnMet: 1.659 ± 0.277
1.614GlnAsn: 1.614 ± 0.305
2.197GlnPro: 2.197 ± 0.361
2.511GlnGln: 2.511 ± 0.585
2.063GlnArg: 2.063 ± 0.311
2.287GlnSer: 2.287 ± 0.321
2.332GlnThr: 2.332 ± 0.399
2.332GlnVal: 2.332 ± 0.397
0.673GlnTrp: 0.673 ± 0.15
1.255GlnTyr: 1.255 ± 0.242
0.0GlnXaa: 0.0 ± 0.0
Arg
5.605ArgAla: 5.605 ± 0.508
0.628ArgCys: 0.628 ± 0.162
4.08ArgAsp: 4.08 ± 0.456
3.632ArgGlu: 3.632 ± 0.429
2.197ArgPhe: 2.197 ± 0.362
4.529ArgGly: 4.529 ± 0.601
1.255ArgHis: 1.255 ± 0.255
3.632ArgIle: 3.632 ± 0.453
5.201ArgLys: 5.201 ± 0.739
3.946ArgLeu: 3.946 ± 0.438
2.197ArgMet: 2.197 ± 0.328
3.094ArgAsn: 3.094 ± 0.351
2.69ArgPro: 2.69 ± 0.301
2.376ArgGln: 2.376 ± 0.296
4.708ArgArg: 4.708 ± 0.593
3.587ArgSer: 3.587 ± 0.458
3.408ArgThr: 3.408 ± 0.352
4.529ArgVal: 4.529 ± 0.417
1.569ArgTrp: 1.569 ± 0.286
2.107ArgTyr: 2.107 ± 0.342
0.0ArgXaa: 0.0 ± 0.0
Ser
5.112SerAla: 5.112 ± 0.392
0.224SerCys: 0.224 ± 0.105
3.184SerAsp: 3.184 ± 0.381
3.139SerGlu: 3.139 ± 0.371
1.569SerPhe: 1.569 ± 0.225
5.784SerGly: 5.784 ± 0.529
1.031SerHis: 1.031 ± 0.254
2.152SerIle: 2.152 ± 0.35
2.376SerLys: 2.376 ± 0.272
3.497SerLeu: 3.497 ± 0.347
1.211SerMet: 1.211 ± 0.256
2.242SerAsn: 2.242 ± 0.248
2.959SerPro: 2.959 ± 0.442
1.838SerGln: 1.838 ± 0.321
3.453SerArg: 3.453 ± 0.384
3.139SerSer: 3.139 ± 0.321
3.273SerThr: 3.273 ± 0.347
2.646SerVal: 2.646 ± 0.388
1.3SerTrp: 1.3 ± 0.238
1.704SerTyr: 1.704 ± 0.278
0.0SerXaa: 0.0 ± 0.0
Thr
5.426ThrAla: 5.426 ± 0.554
0.628ThrCys: 0.628 ± 0.17
4.036ThrAsp: 4.036 ± 0.351
3.408ThrGlu: 3.408 ± 0.502
2.421ThrPhe: 2.421 ± 0.336
6.322ThrGly: 6.322 ± 0.673
1.3ThrHis: 1.3 ± 0.244
2.87ThrIle: 2.87 ± 0.342
2.78ThrLys: 2.78 ± 0.406
3.184ThrLeu: 3.184 ± 0.389
1.166ThrMet: 1.166 ± 0.215
2.063ThrAsn: 2.063 ± 0.278
3.811ThrPro: 3.811 ± 0.493
2.376ThrGln: 2.376 ± 0.346
3.408ThrArg: 3.408 ± 0.369
3.004ThrSer: 3.004 ± 0.294
3.049ThrThr: 3.049 ± 0.388
5.201ThrVal: 5.201 ± 0.464
0.762ThrTrp: 0.762 ± 0.215
1.614ThrTyr: 1.614 ± 0.277
0.0ThrXaa: 0.0 ± 0.0
Val
5.739ValAla: 5.739 ± 0.566
0.986ValCys: 0.986 ± 0.224
3.811ValAsp: 3.811 ± 0.446
5.381ValGlu: 5.381 ± 0.412
2.556ValPhe: 2.556 ± 0.381
4.843ValGly: 4.843 ± 0.581
1.211ValHis: 1.211 ± 0.203
2.735ValIle: 2.735 ± 0.319
3.273ValLys: 3.273 ± 0.495
5.56ValLeu: 5.56 ± 0.463
1.435ValMet: 1.435 ± 0.222
3.004ValAsn: 3.004 ± 0.408
3.632ValPro: 3.632 ± 0.436
3.184ValGln: 3.184 ± 0.345
4.08ValArg: 4.08 ± 0.398
3.273ValSer: 3.273 ± 0.363
4.305ValThr: 4.305 ± 0.51
4.08ValVal: 4.08 ± 0.502
1.525ValTrp: 1.525 ± 0.318
1.39ValTyr: 1.39 ± 0.23
0.0ValXaa: 0.0 ± 0.0
Trp
1.928TrpAla: 1.928 ± 0.257
0.359TrpCys: 0.359 ± 0.129
0.897TrpAsp: 0.897 ± 0.146
0.942TrpGlu: 0.942 ± 0.192
0.897TrpPhe: 0.897 ± 0.175
1.076TrpGly: 1.076 ± 0.174
0.897TrpHis: 0.897 ± 0.163
0.897TrpIle: 0.897 ± 0.219
0.986TrpLys: 0.986 ± 0.181
2.018TrpLeu: 2.018 ± 0.355
0.404TrpMet: 0.404 ± 0.111
0.852TrpAsn: 0.852 ± 0.192
0.897TrpPro: 0.897 ± 0.232
0.807TrpGln: 0.807 ± 0.175
1.39TrpArg: 1.39 ± 0.234
0.986TrpSer: 0.986 ± 0.193
1.39TrpThr: 1.39 ± 0.245
1.39TrpVal: 1.39 ± 0.284
0.448TrpTrp: 0.448 ± 0.177
0.359TrpTyr: 0.359 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.601TyrAla: 2.601 ± 0.345
0.448TyrCys: 0.448 ± 0.155
2.197TyrAsp: 2.197 ± 0.355
1.39TyrGlu: 1.39 ± 0.237
0.986TyrPhe: 0.986 ± 0.206
2.376TyrGly: 2.376 ± 0.336
0.628TyrHis: 0.628 ± 0.179
1.076TyrIle: 1.076 ± 0.216
1.166TyrLys: 1.166 ± 0.327
1.928TyrLeu: 1.928 ± 0.346
0.448TyrMet: 0.448 ± 0.136
1.48TyrAsn: 1.48 ± 0.298
1.39TyrPro: 1.39 ± 0.227
0.762TyrGln: 0.762 ± 0.204
2.152TyrArg: 2.152 ± 0.25
1.166TyrSer: 1.166 ± 0.273
1.794TyrThr: 1.794 ± 0.269
1.883TyrVal: 1.883 ± 0.229
0.493TyrTrp: 0.493 ± 0.163
0.673TyrTyr: 0.673 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 109 proteins (22303 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski