Acinetobacter phage Acj9

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Twarogvirinae; Acajnonavirus; Acinetobacter virus Acj9

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 253 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5EPG6|E5EPG6_9CAUD Uncharacterized protein OS=Acinetobacter phage Acj9 OX=760939 GN=Acj9p032 PE=4 SV=1
MM1 pKa = 7.85SITLNQDD8 pKa = 2.73QVDD11 pKa = 4.71FIGNILLDD19 pKa = 3.93ARR21 pKa = 11.84SLLDD25 pKa = 3.56NVHH28 pKa = 6.56CYY30 pKa = 7.37EE31 pKa = 4.05TEE33 pKa = 3.86EE34 pKa = 4.17YY35 pKa = 9.8EE36 pKa = 4.53RR37 pKa = 11.84LGIAINVLSGQSVEE51 pKa = 4.29EE52 pKa = 3.96ATANCSNEE60 pKa = 3.89EE61 pKa = 3.99EE62 pKa = 4.29

Molecular weight: 6.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5EPH3|E5EPH3_9CAUD Uncharacterized protein OS=Acinetobacter phage Acj9 OX=760939 GN=Acj9p039 PE=4 SV=1
MM1 pKa = 7.11TQTSNYY7 pKa = 9.56EE8 pKa = 3.49IALKK12 pKa = 10.6RR13 pKa = 11.84KK14 pKa = 9.33IDD16 pKa = 3.62ALKK19 pKa = 10.91LSTHH23 pKa = 6.94DD24 pKa = 2.43IWVQRR29 pKa = 11.84KK30 pKa = 9.06RR31 pKa = 11.84NEE33 pKa = 3.84KK34 pKa = 9.0QRR36 pKa = 11.84RR37 pKa = 11.84ALLRR41 pKa = 11.84YY42 pKa = 9.69RR43 pKa = 11.84EE44 pKa = 4.12SHH46 pKa = 5.67HH47 pKa = 6.36AKK49 pKa = 10.22NRR51 pKa = 11.84IAALDD56 pKa = 3.68SRR58 pKa = 11.84DD59 pKa = 3.8ALLLRR64 pKa = 11.84QVMNLQDD71 pKa = 4.12EE72 pKa = 4.67IKK74 pKa = 10.76NAGDD78 pKa = 3.82TICRR82 pKa = 11.84LRR84 pKa = 11.84EE85 pKa = 3.69IRR87 pKa = 11.84RR88 pKa = 11.84GNSKK92 pKa = 8.96RR93 pKa = 11.84TVEE96 pKa = 4.29SVLSQFTLFF105 pKa = 4.17

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

253

0

253

52617

37

1275

208.0

23.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.38 ± 0.171

1.137 ± 0.076

6.158 ± 0.117

6.587 ± 0.179

4.476 ± 0.111

6.089 ± 0.199

2.034 ± 0.087

6.952 ± 0.117

6.926 ± 0.194

8.051 ± 0.149

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.82 ± 0.085

5.43 ± 0.125

3.712 ± 0.092

3.487 ± 0.1

4.402 ± 0.095

6.338 ± 0.129

6.209 ± 0.234

6.72 ± 0.113

1.315 ± 0.067

3.778 ± 0.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski