Amino acid dipepetide frequency for Acinetobacter phage Acj9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.854AlaAla: 5.854 ± 0.388
0.532AlaCys: 0.532 ± 0.108
3.744AlaAsp: 3.744 ± 0.3
4.694AlaGlu: 4.694 ± 0.388
2.547AlaPhe: 2.547 ± 0.198
4.295AlaGly: 4.295 ± 0.406
1.064AlaHis: 1.064 ± 0.144
5.588AlaIle: 5.588 ± 0.348
6.443AlaLys: 6.443 ± 0.42
6.728AlaLeu: 6.728 ± 0.36
2.129AlaMet: 2.129 ± 0.215
4.105AlaAsn: 4.105 ± 0.289
2.699AlaPro: 2.699 ± 0.213
3.117AlaGln: 3.117 ± 0.272
3.117AlaArg: 3.117 ± 0.226
4.751AlaSer: 4.751 ± 0.347
4.238AlaThr: 4.238 ± 0.406
4.447AlaVal: 4.447 ± 0.251
1.064AlaTrp: 1.064 ± 0.166
2.642AlaTyr: 2.642 ± 0.286
0.0AlaXaa: 0.0 ± 0.0
Cys
0.798CysAla: 0.798 ± 0.13
0.171CysCys: 0.171 ± 0.055
0.76CysAsp: 0.76 ± 0.115
0.836CysGlu: 0.836 ± 0.132
0.418CysPhe: 0.418 ± 0.088
0.665CysGly: 0.665 ± 0.138
0.304CysHis: 0.304 ± 0.082
0.684CysIle: 0.684 ± 0.132
0.779CysLys: 0.779 ± 0.127
0.589CysLeu: 0.589 ± 0.109
0.304CysMet: 0.304 ± 0.068
0.57CysAsn: 0.57 ± 0.111
0.57CysPro: 0.57 ± 0.117
0.171CysGln: 0.171 ± 0.068
0.475CysArg: 0.475 ± 0.094
0.988CysSer: 0.988 ± 0.169
0.741CysThr: 0.741 ± 0.111
0.855CysVal: 0.855 ± 0.119
0.247CysTrp: 0.247 ± 0.061
0.437CysTyr: 0.437 ± 0.085
0.0CysXaa: 0.0 ± 0.0
Asp
4.618AspAla: 4.618 ± 0.286
0.76AspCys: 0.76 ± 0.114
3.687AspAsp: 3.687 ± 0.288
4.618AspGlu: 4.618 ± 0.297
3.364AspPhe: 3.364 ± 0.276
4.428AspGly: 4.428 ± 0.283
1.178AspHis: 1.178 ± 0.155
4.01AspIle: 4.01 ± 0.272
3.782AspLys: 3.782 ± 0.318
5.303AspLeu: 5.303 ± 0.358
1.749AspMet: 1.749 ± 0.182
2.927AspAsn: 2.927 ± 0.247
2.528AspPro: 2.528 ± 0.216
1.577AspGln: 1.577 ± 0.148
2.376AspArg: 2.376 ± 0.21
3.155AspSer: 3.155 ± 0.276
3.44AspThr: 3.44 ± 0.294
4.428AspVal: 4.428 ± 0.287
1.007AspTrp: 1.007 ± 0.13
2.642AspTyr: 2.642 ± 0.221
0.0AspXaa: 0.0 ± 0.0
Glu
4.656GluAla: 4.656 ± 0.411
0.798GluCys: 0.798 ± 0.118
3.535GluAsp: 3.535 ± 0.306
3.573GluGlu: 3.573 ± 0.299
3.44GluPhe: 3.44 ± 0.244
3.212GluGly: 3.212 ± 0.28
1.311GluHis: 1.311 ± 0.176
5.797GluIle: 5.797 ± 0.344
3.991GluLys: 3.991 ± 0.26
6.139GluLeu: 6.139 ± 0.364
2.167GluMet: 2.167 ± 0.238
3.573GluAsn: 3.573 ± 0.263
1.882GluPro: 1.882 ± 0.218
2.604GluGln: 2.604 ± 0.231
3.269GluArg: 3.269 ± 0.275
3.915GluSer: 3.915 ± 0.313
3.991GluThr: 3.991 ± 0.316
4.39GluVal: 4.39 ± 0.279
0.817GluTrp: 0.817 ± 0.12
2.813GluTyr: 2.813 ± 0.275
0.0GluXaa: 0.0 ± 0.0
Phe
3.193PheAla: 3.193 ± 0.248
0.494PheCys: 0.494 ± 0.088
3.953PheAsp: 3.953 ± 0.28
3.915PheGlu: 3.915 ± 0.309
1.444PhePhe: 1.444 ± 0.173
3.212PheGly: 3.212 ± 0.251
0.817PheHis: 0.817 ± 0.136
3.25PheIle: 3.25 ± 0.219
3.307PheLys: 3.307 ± 0.233
2.832PheLeu: 2.832 ± 0.262
1.007PheMet: 1.007 ± 0.135
2.452PheAsn: 2.452 ± 0.225
1.577PhePro: 1.577 ± 0.173
1.311PheGln: 1.311 ± 0.171
1.52PheArg: 1.52 ± 0.163
2.756PheSer: 2.756 ± 0.221
2.509PheThr: 2.509 ± 0.236
3.193PheVal: 3.193 ± 0.237
0.456PheTrp: 0.456 ± 0.101
1.558PheTyr: 1.558 ± 0.214
0.0PheXaa: 0.0 ± 0.0
Gly
4.352GlyAla: 4.352 ± 0.355
0.608GlyCys: 0.608 ± 0.121
3.915GlyAsp: 3.915 ± 0.242
3.44GlyGlu: 3.44 ± 0.23
2.509GlyPhe: 2.509 ± 0.253
2.984GlyGly: 2.984 ± 0.355
1.026GlyHis: 1.026 ± 0.178
4.295GlyIle: 4.295 ± 0.311
3.668GlyLys: 3.668 ± 0.285
5.284GlyLeu: 5.284 ± 0.331
1.806GlyMet: 1.806 ± 0.192
3.573GlyAsn: 3.573 ± 0.422
1.806GlyPro: 1.806 ± 0.195
2.053GlyGln: 2.053 ± 0.18
2.414GlyArg: 2.414 ± 0.202
4.295GlySer: 4.295 ± 0.305
4.979GlyThr: 4.979 ± 0.531
4.485GlyVal: 4.485 ± 0.301
0.874GlyTrp: 0.874 ± 0.148
2.528GlyTyr: 2.528 ± 0.212
0.0GlyXaa: 0.0 ± 0.0
His
1.33HisAla: 1.33 ± 0.17
0.304HisCys: 0.304 ± 0.084
1.311HisAsp: 1.311 ± 0.167
0.988HisGlu: 0.988 ± 0.134
1.007HisPhe: 1.007 ± 0.144
1.273HisGly: 1.273 ± 0.19
0.513HisHis: 0.513 ± 0.1
1.73HisIle: 1.73 ± 0.191
1.653HisLys: 1.653 ± 0.197
1.73HisLeu: 1.73 ± 0.208
0.57HisMet: 0.57 ± 0.097
0.912HisAsn: 0.912 ± 0.133
0.893HisPro: 0.893 ± 0.124
0.627HisGln: 0.627 ± 0.113
0.779HisArg: 0.779 ± 0.126
1.387HisSer: 1.387 ± 0.16
1.064HisThr: 1.064 ± 0.119
1.349HisVal: 1.349 ± 0.151
0.19HisTrp: 0.19 ± 0.066
0.722HisTyr: 0.722 ± 0.109
0.0HisXaa: 0.0 ± 0.0
Ile
5.455IleAla: 5.455 ± 0.34
0.931IleCys: 0.931 ± 0.14
5.075IleAsp: 5.075 ± 0.379
5.284IleGlu: 5.284 ± 0.301
2.718IlePhe: 2.718 ± 0.214
3.858IleGly: 3.858 ± 0.29
1.273IleHis: 1.273 ± 0.155
3.972IleIle: 3.972 ± 0.276
4.77IleLys: 4.77 ± 0.323
5.436IleLeu: 5.436 ± 0.333
1.634IleMet: 1.634 ± 0.198
4.295IleAsn: 4.295 ± 0.323
2.224IlePro: 2.224 ± 0.221
2.452IleGln: 2.452 ± 0.17
3.288IleArg: 3.288 ± 0.284
4.542IleSer: 4.542 ± 0.303
4.542IleThr: 4.542 ± 0.304
4.656IleVal: 4.656 ± 0.25
0.817IleTrp: 0.817 ± 0.133
2.3IleTyr: 2.3 ± 0.209
0.0IleXaa: 0.0 ± 0.0
Lys
5.379LysAla: 5.379 ± 0.37
0.57LysCys: 0.57 ± 0.112
4.333LysAsp: 4.333 ± 0.34
4.2LysGlu: 4.2 ± 0.266
3.326LysPhe: 3.326 ± 0.244
4.067LysGly: 4.067 ± 0.261
1.996LysHis: 1.996 ± 0.227
4.922LysIle: 4.922 ± 0.316
3.649LysLys: 3.649 ± 0.3
6.177LysLeu: 6.177 ± 0.34
2.528LysMet: 2.528 ± 0.22
3.592LysAsn: 3.592 ± 0.259
2.509LysPro: 2.509 ± 0.201
1.977LysGln: 1.977 ± 0.192
3.307LysArg: 3.307 ± 0.273
4.333LysSer: 4.333 ± 0.327
3.991LysThr: 3.991 ± 0.249
4.333LysVal: 4.333 ± 0.305
1.026LysTrp: 1.026 ± 0.135
3.041LysTyr: 3.041 ± 0.242
0.0LysXaa: 0.0 ± 0.0
Leu
6.652LeuAla: 6.652 ± 0.342
1.026LeuCys: 1.026 ± 0.165
5.227LeuAsp: 5.227 ± 0.352
5.645LeuGlu: 5.645 ± 0.347
3.364LeuPhe: 3.364 ± 0.249
4.675LeuGly: 4.675 ± 0.275
1.92LeuHis: 1.92 ± 0.194
4.884LeuIle: 4.884 ± 0.332
6.348LeuLys: 6.348 ± 0.397
5.417LeuLeu: 5.417 ± 0.303
2.433LeuMet: 2.433 ± 0.233
4.371LeuAsn: 4.371 ± 0.297
3.459LeuPro: 3.459 ± 0.209
2.832LeuGln: 2.832 ± 0.205
3.573LeuArg: 3.573 ± 0.235
5.17LeuSer: 5.17 ± 0.314
5.094LeuThr: 5.094 ± 0.256
4.903LeuVal: 4.903 ± 0.317
0.779LeuTrp: 0.779 ± 0.108
2.737LeuTyr: 2.737 ± 0.239
0.0LeuXaa: 0.0 ± 0.0
Met
2.186MetAla: 2.186 ± 0.208
0.247MetCys: 0.247 ± 0.056
1.425MetAsp: 1.425 ± 0.143
1.501MetGlu: 1.501 ± 0.192
1.33MetPhe: 1.33 ± 0.144
1.577MetGly: 1.577 ± 0.196
0.494MetHis: 0.494 ± 0.082
1.672MetIle: 1.672 ± 0.16
2.604MetLys: 2.604 ± 0.233
2.091MetLeu: 2.091 ± 0.214
0.76MetMet: 0.76 ± 0.124
1.596MetAsn: 1.596 ± 0.179
1.026MetPro: 1.026 ± 0.144
1.083MetGln: 1.083 ± 0.14
1.52MetArg: 1.52 ± 0.174
2.072MetSer: 2.072 ± 0.207
1.901MetThr: 1.901 ± 0.165
1.749MetVal: 1.749 ± 0.187
0.247MetTrp: 0.247 ± 0.066
1.121MetTyr: 1.121 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
3.896AsnAla: 3.896 ± 0.262
0.722AsnCys: 0.722 ± 0.113
2.718AsnAsp: 2.718 ± 0.23
3.687AsnGlu: 3.687 ± 0.284
2.756AsnPhe: 2.756 ± 0.182
4.2AsnGly: 4.2 ± 0.349
1.14AsnHis: 1.14 ± 0.145
3.744AsnIle: 3.744 ± 0.234
3.155AsnLys: 3.155 ± 0.237
4.922AsnLeu: 4.922 ± 0.226
1.52AsnMet: 1.52 ± 0.143
3.136AsnAsn: 3.136 ± 0.285
2.452AsnPro: 2.452 ± 0.27
1.787AsnGln: 1.787 ± 0.209
2.604AsnArg: 2.604 ± 0.212
3.003AsnSer: 3.003 ± 0.237
3.041AsnThr: 3.041 ± 0.253
3.497AsnVal: 3.497 ± 0.233
0.456AsnTrp: 0.456 ± 0.092
1.863AsnTyr: 1.863 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
2.604ProAla: 2.604 ± 0.246
0.399ProCys: 0.399 ± 0.086
2.452ProAsp: 2.452 ± 0.252
2.756ProGlu: 2.756 ± 0.237
1.634ProPhe: 1.634 ± 0.193
2.737ProGly: 2.737 ± 0.218
0.665ProHis: 0.665 ± 0.119
2.376ProIle: 2.376 ± 0.242
2.965ProLys: 2.965 ± 0.274
2.414ProLeu: 2.414 ± 0.202
0.836ProMet: 0.836 ± 0.122
2.186ProAsn: 2.186 ± 0.225
1.045ProPro: 1.045 ± 0.146
1.349ProGln: 1.349 ± 0.168
1.501ProArg: 1.501 ± 0.162
2.262ProSer: 2.262 ± 0.175
2.281ProThr: 2.281 ± 0.211
2.623ProVal: 2.623 ± 0.227
0.684ProTrp: 0.684 ± 0.118
1.33ProTyr: 1.33 ± 0.159
0.0ProXaa: 0.0 ± 0.0
Gln
2.642GlnAla: 2.642 ± 0.247
0.418GlnCys: 0.418 ± 0.104
1.558GlnAsp: 1.558 ± 0.168
2.281GlnGlu: 2.281 ± 0.181
1.692GlnPhe: 1.692 ± 0.195
2.015GlnGly: 2.015 ± 0.205
0.855GlnHis: 0.855 ± 0.128
2.68GlnIle: 2.68 ± 0.26
1.634GlnLys: 1.634 ± 0.171
3.288GlnLeu: 3.288 ± 0.239
1.216GlnMet: 1.216 ± 0.148
1.539GlnAsn: 1.539 ± 0.172
1.007GlnPro: 1.007 ± 0.129
1.121GlnGln: 1.121 ± 0.137
1.672GlnArg: 1.672 ± 0.163
2.167GlnSer: 2.167 ± 0.178
2.433GlnThr: 2.433 ± 0.237
2.414GlnVal: 2.414 ± 0.226
0.608GlnTrp: 0.608 ± 0.106
1.634GlnTyr: 1.634 ± 0.174
0.0GlnXaa: 0.0 ± 0.0
Arg
3.326ArgAla: 3.326 ± 0.241
0.551ArgCys: 0.551 ± 0.106
2.87ArgAsp: 2.87 ± 0.207
2.775ArgGlu: 2.775 ± 0.218
2.205ArgPhe: 2.205 ± 0.194
2.547ArgGly: 2.547 ± 0.18
0.912ArgHis: 0.912 ± 0.134
3.345ArgIle: 3.345 ± 0.251
3.193ArgLys: 3.193 ± 0.215
3.573ArgLeu: 3.573 ± 0.308
1.273ArgMet: 1.273 ± 0.151
2.148ArgAsn: 2.148 ± 0.189
1.501ArgPro: 1.501 ± 0.158
1.711ArgGln: 1.711 ± 0.193
1.711ArgArg: 1.711 ± 0.179
2.832ArgSer: 2.832 ± 0.219
2.509ArgThr: 2.509 ± 0.199
3.155ArgVal: 3.155 ± 0.231
0.456ArgTrp: 0.456 ± 0.103
1.425ArgTyr: 1.425 ± 0.142
0.0ArgXaa: 0.0 ± 0.0
Ser
3.972SerAla: 3.972 ± 0.29
0.741SerCys: 0.741 ± 0.146
3.82SerAsp: 3.82 ± 0.2
3.877SerGlu: 3.877 ± 0.238
2.965SerPhe: 2.965 ± 0.233
4.143SerGly: 4.143 ± 0.338
1.216SerHis: 1.216 ± 0.141
4.789SerIle: 4.789 ± 0.291
4.789SerLys: 4.789 ± 0.29
4.732SerLeu: 4.732 ± 0.264
1.501SerMet: 1.501 ± 0.19
3.649SerAsn: 3.649 ± 0.252
2.338SerPro: 2.338 ± 0.239
2.034SerGln: 2.034 ± 0.21
3.06SerArg: 3.06 ± 0.257
4.238SerSer: 4.238 ± 0.317
4.58SerThr: 4.58 ± 0.365
3.972SerVal: 3.972 ± 0.311
0.665SerTrp: 0.665 ± 0.115
2.3SerTyr: 2.3 ± 0.193
0.0SerXaa: 0.0 ± 0.0
Thr
4.295ThrAla: 4.295 ± 0.382
0.608ThrCys: 0.608 ± 0.125
3.535ThrAsp: 3.535 ± 0.298
3.782ThrGlu: 3.782 ± 0.343
2.87ThrPhe: 2.87 ± 0.269
4.124ThrGly: 4.124 ± 0.371
1.254ThrHis: 1.254 ± 0.131
4.409ThrIle: 4.409 ± 0.29
4.067ThrLys: 4.067 ± 0.267
5.017ThrLeu: 5.017 ± 0.332
1.311ThrMet: 1.311 ± 0.159
3.364ThrAsn: 3.364 ± 0.285
2.737ThrPro: 2.737 ± 0.239
2.889ThrGln: 2.889 ± 0.273
2.566ThrArg: 2.566 ± 0.216
3.953ThrSer: 3.953 ± 0.35
3.896ThrThr: 3.896 ± 0.32
3.763ThrVal: 3.763 ± 0.295
1.14ThrTrp: 1.14 ± 0.188
2.509ThrTyr: 2.509 ± 0.211
0.0ThrXaa: 0.0 ± 0.0
Val
5.113ValAla: 5.113 ± 0.37
0.836ValCys: 0.836 ± 0.126
4.162ValAsp: 4.162 ± 0.246
4.694ValGlu: 4.694 ± 0.339
2.946ValPhe: 2.946 ± 0.235
3.915ValGly: 3.915 ± 0.269
1.311ValHis: 1.311 ± 0.163
4.257ValIle: 4.257 ± 0.274
5.303ValLys: 5.303 ± 0.323
5.189ValLeu: 5.189 ± 0.276
1.901ValMet: 1.901 ± 0.169
3.212ValAsn: 3.212 ± 0.31
3.022ValPro: 3.022 ± 0.228
2.319ValGln: 2.319 ± 0.234
2.946ValArg: 2.946 ± 0.22
4.01ValSer: 4.01 ± 0.276
3.725ValThr: 3.725 ± 0.265
4.998ValVal: 4.998 ± 0.312
0.798ValTrp: 0.798 ± 0.139
2.547ValTyr: 2.547 ± 0.224
0.0ValXaa: 0.0 ± 0.0
Trp
0.779TrpAla: 0.779 ± 0.119
0.228TrpCys: 0.228 ± 0.071
1.026TrpAsp: 1.026 ± 0.125
0.874TrpGlu: 0.874 ± 0.13
0.513TrpPhe: 0.513 ± 0.101
0.608TrpGly: 0.608 ± 0.125
0.266TrpHis: 0.266 ± 0.068
0.741TrpIle: 0.741 ± 0.094
1.026TrpLys: 1.026 ± 0.143
0.95TrpLeu: 0.95 ± 0.14
0.399TrpMet: 0.399 ± 0.087
0.76TrpAsn: 0.76 ± 0.113
0.456TrpPro: 0.456 ± 0.091
0.456TrpGln: 0.456 ± 0.095
0.532TrpArg: 0.532 ± 0.105
0.703TrpSer: 0.703 ± 0.104
0.855TrpThr: 0.855 ± 0.132
1.216TrpVal: 1.216 ± 0.135
0.228TrpTrp: 0.228 ± 0.06
0.532TrpTyr: 0.532 ± 0.074
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.699TyrAla: 2.699 ± 0.208
0.418TyrCys: 0.418 ± 0.09
2.471TyrAsp: 2.471 ± 0.216
2.452TyrGlu: 2.452 ± 0.19
1.711TyrPhe: 1.711 ± 0.174
2.357TyrGly: 2.357 ± 0.194
0.817TyrHis: 0.817 ± 0.123
2.376TyrIle: 2.376 ± 0.21
2.129TyrLys: 2.129 ± 0.169
2.699TyrLeu: 2.699 ± 0.248
1.14TyrMet: 1.14 ± 0.13
2.338TyrAsn: 2.338 ± 0.222
1.387TyrPro: 1.387 ± 0.153
1.406TyrGln: 1.406 ± 0.152
1.787TyrArg: 1.787 ± 0.17
2.851TyrSer: 2.851 ± 0.218
2.281TyrThr: 2.281 ± 0.182
2.775TyrVal: 2.775 ± 0.216
0.589TyrTrp: 0.589 ± 0.114
1.102TyrTyr: 1.102 ± 0.183
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 253 proteins (52617 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski