Helicobacter macacae MIT 99-5501

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae; Helicobacter; Helicobacter macacae

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1946 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V8C617|V8C617_9HELI Uncharacterized protein OS=Helicobacter macacae MIT 99-5501 OX=1357400 GN=HMPREF2086_01757 PE=4 SV=1
MM1 pKa = 8.06SLMINDD7 pKa = 3.48EE8 pKa = 4.84CIACDD13 pKa = 3.56ACLEE17 pKa = 4.19EE18 pKa = 5.17CPNEE22 pKa = 4.21AIIEE26 pKa = 4.16GEE28 pKa = 4.04PIYY31 pKa = 10.71RR32 pKa = 11.84IDD34 pKa = 4.18PDD36 pKa = 3.95CCTEE40 pKa = 3.9CVGYY44 pKa = 10.48YY45 pKa = 10.13DD46 pKa = 4.89EE47 pKa = 5.3PSCVSACPKK56 pKa = 10.14DD57 pKa = 5.35AIMPDD62 pKa = 3.45PDD64 pKa = 3.48NAEE67 pKa = 4.01SLEE70 pKa = 3.94EE71 pKa = 3.87LKK73 pKa = 11.09YY74 pKa = 10.72KK75 pKa = 10.82YY76 pKa = 9.92EE77 pKa = 3.84QLKK80 pKa = 10.78NKK82 pKa = 8.72EE83 pKa = 3.98

Molecular weight: 9.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V8C6Y3|V8C6Y3_9HELI Uncharacterized protein OS=Helicobacter macacae MIT 99-5501 OX=1357400 GN=HMPREF2086_01631 PE=4 SV=1
MM1 pKa = 7.47ISKK4 pKa = 10.88GNLFWGMRR12 pKa = 11.84FRR14 pKa = 11.84TCKK17 pKa = 10.55NFWEE21 pKa = 4.32QQTPSLVLSQKK32 pKa = 10.01FLQIQKK38 pKa = 8.31PHH40 pKa = 5.55RR41 pKa = 11.84HH42 pKa = 5.49YY43 pKa = 10.99FDD45 pKa = 4.01CCFEE49 pKa = 4.65LSLSQITRR57 pKa = 11.84IAIHH61 pKa = 6.07LVNRR65 pKa = 11.84LRR67 pKa = 11.84IKK69 pKa = 10.78CEE71 pKa = 3.39LLFRR75 pKa = 11.84HH76 pKa = 6.2CEE78 pKa = 3.66ILRR81 pKa = 11.84SKK83 pKa = 10.33IVAIQNSPSIAEE95 pKa = 4.28GVRR98 pKa = 11.84VWVIRR103 pKa = 11.84IFVIASEE110 pKa = 4.81CVAQTRR116 pKa = 11.84QSTFARR122 pKa = 11.84HH123 pKa = 5.42CEE125 pKa = 3.35IRR127 pKa = 11.84QRR129 pKa = 11.84RR130 pKa = 11.84IEE132 pKa = 4.52AIQKK136 pKa = 10.06KK137 pKa = 7.53MWIATNRR144 pKa = 11.84YY145 pKa = 8.86AIPTMTMSVARR156 pKa = 11.84QQ157 pKa = 3.04

Molecular weight: 18.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1946

0

1946

668153

29

2899

343.3

38.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.745 ± 0.055

1.271 ± 0.02

5.068 ± 0.043

6.184 ± 0.064

5.103 ± 0.045

6.294 ± 0.066

1.856 ± 0.021

7.672 ± 0.047

8.09 ± 0.068

9.849 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.968 ± 0.025

5.278 ± 0.056

3.323 ± 0.029

4.009 ± 0.039

3.871 ± 0.031

8.159 ± 0.058

4.728 ± 0.047

5.259 ± 0.055

0.734 ± 0.014

3.54 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski