Silvanigrella aquatica
Average proteome isoelectric point is 7.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2865 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L4CXN0|A0A1L4CXN0_9PROT Uncharacterized protein OS=Silvanigrella aquatica OX=1915309 GN=AXG55_01630 PE=4 SV=1
MM1 pKa = 6.55 NQEE4 pKa = 3.79 YY5 pKa = 10.82 GNWGWEE11 pKa = 3.66 IFVYY15 pKa = 10.23 PSYY18 pKa = 10.84 IVVAIILLSYY28 pKa = 10.05 IGYY31 pKa = 10.17 SYY33 pKa = 11.21 FSLINSLKK41 pKa = 10.39 DD42 pKa = 3.28 MRR44 pKa = 11.84 NEE46 pKa = 4.56 GYY48 pKa = 10.39 FEE50 pKa = 5.38 NNDD53 pKa = 3.32 DD54 pKa = 4.45 SNFNFSDD61 pKa = 3.52 KK62 pKa = 10.97 SSEE65 pKa = 3.96 SDD67 pKa = 3.32 LSGKK71 pKa = 10.68 GSDD74 pKa = 3.48 SS75 pKa = 3.31
Molecular weight: 8.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.855
IPC2_protein 3.999
IPC_protein 3.872
Toseland 3.681
ProMoST 4.037
Dawson 3.859
Bjellqvist 4.024
Wikipedia 3.795
Rodwell 3.706
Grimsley 3.592
Solomon 3.834
Lehninger 3.783
Nozaki 3.999
DTASelect 4.164
Thurlkill 3.745
EMBOSS 3.808
Sillero 3.986
Patrickios 0.401
IPC_peptide 3.834
IPC2_peptide 3.961
IPC2.peptide.svr19 3.906
Protein with the highest isoelectric point:
>tr|A0A1L4CXD5|A0A1L4CXD5_9PROT Uncharacterized protein OS=Silvanigrella aquatica OX=1915309 GN=AXG55_01150 PE=4 SV=1
MM1 pKa = 7.24 VVRR4 pKa = 11.84 KK5 pKa = 9.29 RR6 pKa = 11.84 KK7 pKa = 9.53 KK8 pKa = 10.32 AGASKK13 pKa = 10.4 AKK15 pKa = 10.3 KK16 pKa = 8.53 VTTGKK21 pKa = 10.12 KK22 pKa = 8.91 RR23 pKa = 11.84 RR24 pKa = 11.84 KK25 pKa = 8.09 SAKK28 pKa = 9.94 AKK30 pKa = 8.83 VAKK33 pKa = 9.83 KK34 pKa = 10.46 RR35 pKa = 11.84 KK36 pKa = 5.37 TTAKK40 pKa = 9.83 KK41 pKa = 8.28 ATRR44 pKa = 11.84 RR45 pKa = 11.84 KK46 pKa = 7.61 TKK48 pKa = 10.47 AKK50 pKa = 9.1 RR51 pKa = 11.84 TTKK54 pKa = 10.57 KK55 pKa = 10.38 AGAPKK60 pKa = 9.94 KK61 pKa = 10.33 RR62 pKa = 11.84 KK63 pKa = 7.33 TKK65 pKa = 10.34 RR66 pKa = 11.84 KK67 pKa = 7.23 TVGKK71 pKa = 9.64 RR72 pKa = 11.84 KK73 pKa = 7.78 TVGKK77 pKa = 10.2 RR78 pKa = 11.84 KK79 pKa = 7.62 TAGKK83 pKa = 10.11 RR84 pKa = 11.84 KK85 pKa = 8.0 TSAKK89 pKa = 9.44 KK90 pKa = 10.18 RR91 pKa = 11.84 KK92 pKa = 9.53 SKK94 pKa = 10.75 LSLVAKK100 pKa = 6.88 PTRR103 pKa = 11.84 KK104 pKa = 9.7 KK105 pKa = 9.3 RR106 pKa = 11.84 TKK108 pKa = 9.93 KK109 pKa = 9.58 ASKK112 pKa = 10.28 RR113 pKa = 11.84 KK114 pKa = 8.05 ATAKK118 pKa = 10.25 KK119 pKa = 8.88 VVARR123 pKa = 11.84 KK124 pKa = 9.92 SSEE127 pKa = 3.76 KK128 pKa = 10.13 HH129 pKa = 5.35 LKK131 pKa = 9.4 KK132 pKa = 10.75 AGG134 pKa = 3.41
Molecular weight: 15.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.49
IPC2_protein 11.257
IPC_protein 12.749
Toseland 12.939
ProMoST 13.408
Dawson 12.954
Bjellqvist 12.925
Wikipedia 13.393
Rodwell 13.013
Grimsley 12.983
Solomon 13.422
Lehninger 13.32
Nozaki 12.939
DTASelect 12.925
Thurlkill 12.939
EMBOSS 13.437
Sillero 12.939
Patrickios 12.72
IPC_peptide 13.422
IPC2_peptide 12.398
IPC2.peptide.svr19 9.066
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2865
0
2865
1001728
37
4281
349.6
39.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.116 ± 0.064
1.173 ± 0.016
4.616 ± 0.032
6.411 ± 0.054
5.399 ± 0.048
5.539 ± 0.048
1.96 ± 0.025
8.755 ± 0.056
8.124 ± 0.07
9.729 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.13 ± 0.023
6.665 ± 0.061
3.582 ± 0.033
3.54 ± 0.026
3.438 ± 0.031
7.836 ± 0.067
4.909 ± 0.08
5.417 ± 0.04
0.991 ± 0.015
3.669 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here