Sphingobacterium lactis

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Sphingobacteriia; Sphingobacteriales; Sphingobacteriaceae; Sphingobacterium

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3523 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5TS81|A0A1H5TS81_9SPHI Transcriptional regulator ArsR family OS=Sphingobacterium lactis OX=797291 GN=SAMN05421877_10267 PE=4 SV=1
MM1 pKa = 7.77ANWCNNWVVFEE12 pKa = 4.32GTAEE16 pKa = 4.56AIEE19 pKa = 4.34QITKK23 pKa = 10.17LFKK26 pKa = 11.18SMAEE30 pKa = 3.89QEE32 pKa = 4.29QKK34 pKa = 11.02DD35 pKa = 3.85DD36 pKa = 4.27CGQLPDD42 pKa = 4.99FVQDD46 pKa = 2.99THH48 pKa = 8.32GDD50 pKa = 3.44YY51 pKa = 10.62FYY53 pKa = 11.16NISQDD58 pKa = 3.51NEE60 pKa = 4.29SAGVFQYY67 pKa = 7.25EE68 pKa = 4.91TKK70 pKa = 10.2WSPNTDD76 pKa = 2.87AVKK79 pKa = 10.55QIAEE83 pKa = 4.05HH84 pKa = 5.9FKK86 pKa = 11.12VNFTQDD92 pKa = 3.63YY93 pKa = 9.93EE94 pKa = 4.3EE95 pKa = 5.16LGCLVYY101 pKa = 10.63GQAIFEE107 pKa = 4.27EE108 pKa = 5.99GILTDD113 pKa = 3.61TCLDD117 pKa = 3.63SQDD120 pKa = 3.46FDD122 pKa = 4.42SYY124 pKa = 11.79EE125 pKa = 4.21LDD127 pKa = 3.79EE128 pKa = 4.65EE129 pKa = 4.42TDD131 pKa = 3.52TYY133 pKa = 11.13HH134 pKa = 7.57FEE136 pKa = 3.75GTEE139 pKa = 3.91YY140 pKa = 10.97DD141 pKa = 4.18SEE143 pKa = 4.28WEE145 pKa = 3.97ILDD148 pKa = 3.59TLLEE152 pKa = 4.4RR153 pKa = 11.84KK154 pKa = 9.52SEE156 pKa = 4.07NQLNTIKK163 pKa = 10.43II164 pKa = 3.87

Molecular weight: 19.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H6C3J4|A0A1H6C3J4_9SPHI Uncharacterized protein OS=Sphingobacterium lactis OX=797291 GN=SAMN05421877_11314 PE=4 SV=1
MM1 pKa = 7.27GVLVLMVCSGVSKK14 pKa = 10.46IVTPLRR20 pKa = 11.84GWHH23 pKa = 4.89VRR25 pKa = 11.84YY26 pKa = 9.37PNRR29 pKa = 11.84SLYY32 pKa = 10.69LLPKK36 pKa = 9.49CGKK39 pKa = 8.6GTVKK43 pKa = 10.19CFCAYY48 pKa = 10.49GMFRR52 pKa = 11.84HH53 pKa = 6.54KK54 pKa = 10.73SATTQAHH61 pKa = 5.53YY62 pKa = 10.74KK63 pKa = 9.87RR64 pKa = 11.84LAPACEE70 pKa = 3.94LTRR73 pKa = 11.84LGEE76 pKa = 3.96ILRR79 pKa = 11.84GNGRR83 pKa = 11.84ALRR86 pKa = 11.84AHH88 pKa = 6.55SLSKK92 pKa = 10.51SLPYY96 pKa = 10.22FNRR99 pKa = 11.84PMLAQEE105 pKa = 4.01LCGRR109 pKa = 11.84ATRR112 pKa = 11.84WISCAFGMRR121 pKa = 11.84QHH123 pKa = 6.67SLPKK127 pKa = 9.27SLPYY131 pKa = 10.15FMCTTLAQEE140 pKa = 3.77LCGRR144 pKa = 11.84VVRR147 pKa = 11.84RR148 pKa = 11.84ISCAQGMRR156 pKa = 11.84QHH158 pKa = 7.48PYY160 pKa = 9.81HH161 pKa = 6.64SRR163 pKa = 3.22

Molecular weight: 18.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3523

0

3523

1183612

30

1928

336.0

37.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.31 ± 0.04

0.696 ± 0.012

5.552 ± 0.029

6.311 ± 0.044

5.002 ± 0.028

6.81 ± 0.043

1.94 ± 0.023

7.223 ± 0.035

6.893 ± 0.036

9.494 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.447 ± 0.018

5.432 ± 0.039

3.719 ± 0.022

3.959 ± 0.028

4.088 ± 0.027

6.138 ± 0.032

5.349 ± 0.028

6.381 ± 0.03

1.148 ± 0.015

4.11 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski