Synechococcus phage S-CBWM1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Aokuangvirus; Synechococcus virus SCBWM1

Average proteome isoelectric point is 5.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 178 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G1L3M6|A0A3G1L3M6_9CAUD Putative tail protein OS=Synechococcus phage S-CBWM1 OX=2053653 GN=SCBWM1_gp93 PE=4 SV=1
MM1 pKa = 7.08TQTTLLFPDD10 pKa = 3.92YY11 pKa = 8.28PTAVSAAKK19 pKa = 10.51SLGFWEE25 pKa = 4.43DD26 pKa = 3.58QIIQTPIYY34 pKa = 8.99DD35 pKa = 3.88TEE37 pKa = 4.42AEE39 pKa = 4.05PDD41 pKa = 3.39AEE43 pKa = 4.44GNYY46 pKa = 9.94PPLLDD51 pKa = 4.7EE52 pKa = 4.92NGQPSFTEE60 pKa = 4.09TTEE63 pKa = 4.14GRR65 pKa = 11.84LKK67 pKa = 10.12TAGQTIPEE75 pKa = 4.26VGSPFSWMIDD85 pKa = 3.52EE86 pKa = 4.97IGADD90 pKa = 3.36PVVIPGTYY98 pKa = 9.92DD99 pKa = 3.11EE100 pKa = 5.08EE101 pKa = 5.21GNEE104 pKa = 3.74IVAPSRR110 pKa = 11.84LLGYY114 pKa = 9.23VVNVTGEE121 pKa = 4.13LPEE124 pKa = 4.06TALAYY129 pKa = 9.81SIPYY133 pKa = 9.65GSAGRR138 pKa = 11.84LFSGSEE144 pKa = 4.07AEE146 pKa = 4.15PFSYY150 pKa = 10.67EE151 pKa = 4.39PIPDD155 pKa = 3.33KK156 pKa = 10.78PGYY159 pKa = 10.13VLAKK163 pKa = 10.23GG164 pKa = 3.51

Molecular weight: 17.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G1L3K3|A0A3G1L3K3_9CAUD Mitochondrial DNA polymerase catalytic subunit OS=Synechococcus phage S-CBWM1 OX=2053653 GN=SCBWM1_gp77 PE=4 SV=1
MM1 pKa = 7.15FRR3 pKa = 11.84INMVRR8 pKa = 11.84AFATILLLPSSAAAAATIEE27 pKa = 4.21RR28 pKa = 11.84EE29 pKa = 4.11LPLFPSEE36 pKa = 4.16NDD38 pKa = 2.79ASRR41 pKa = 11.84IEE43 pKa = 4.07RR44 pKa = 11.84FLPKK48 pKa = 10.1EE49 pKa = 4.04SNLGVRR55 pKa = 11.84MAYY58 pKa = 9.75TNTTRR63 pKa = 11.84ASWYY67 pKa = 10.53GPGFYY72 pKa = 10.76GNRR75 pKa = 11.84TANGEE80 pKa = 4.24IYY82 pKa = 10.49SGTGMTAAHH91 pKa = 7.19KK92 pKa = 9.08YY93 pKa = 10.52LPFGTRR99 pKa = 11.84LRR101 pKa = 11.84VTNHH105 pKa = 5.62RR106 pKa = 11.84NGRR109 pKa = 11.84SVVVRR114 pKa = 11.84INDD117 pKa = 3.56RR118 pKa = 11.84GPYY121 pKa = 9.09VSGRR125 pKa = 11.84GIDD128 pKa = 3.6LSEE131 pKa = 3.91RR132 pKa = 11.84AARR135 pKa = 11.84ALGISGVDD143 pKa = 3.14TVTLTKK149 pKa = 10.77VNN151 pKa = 3.65

Molecular weight: 16.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

178

0

178

41271

45

1573

231.9

25.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.565 ± 0.31

1.027 ± 0.078

5.58 ± 0.134

7.555 ± 0.278

4.226 ± 0.151

8.151 ± 0.271

1.526 ± 0.091

5.522 ± 0.107

4.967 ± 0.236

8.454 ± 0.222

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.132 ± 0.114

4.335 ± 0.132

5.115 ± 0.179

3.077 ± 0.119

5.561 ± 0.176

7.722 ± 0.205

5.755 ± 0.234

6.775 ± 0.17

1.551 ± 0.095

3.404 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski