Amino acid dipepetide frequency for Synechococcus phage S-CBWM1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.245AlaAla: 7.245 ± 0.721
0.654AlaCys: 0.654 ± 0.122
4.047AlaAsp: 4.047 ± 0.279
5.791AlaGlu: 5.791 ± 0.422
2.811AlaPhe: 2.811 ± 0.252
5.961AlaGly: 5.961 ± 0.581
1.284AlaHis: 1.284 ± 0.175
4.822AlaIle: 4.822 ± 0.377
4.773AlaLys: 4.773 ± 0.551
6.978AlaLeu: 6.978 ± 0.425
2.205AlaMet: 2.205 ± 0.26
3.513AlaAsn: 3.513 ± 0.309
2.98AlaPro: 2.98 ± 0.351
2.375AlaGln: 2.375 ± 0.274
3.295AlaArg: 3.295 ± 0.339
5.016AlaSer: 5.016 ± 0.433
4.798AlaThr: 4.798 ± 0.539
3.756AlaVal: 3.756 ± 0.344
0.897AlaTrp: 0.897 ± 0.175
2.447AlaTyr: 2.447 ± 0.29
0.0AlaXaa: 0.0 ± 0.0
Cys
0.485CysAla: 0.485 ± 0.099
0.145CysCys: 0.145 ± 0.057
0.509CysAsp: 0.509 ± 0.108
0.897CysGlu: 0.897 ± 0.183
0.46CysPhe: 0.46 ± 0.114
0.945CysGly: 0.945 ± 0.142
0.218CysHis: 0.218 ± 0.074
0.46CysIle: 0.46 ± 0.126
0.363CysLys: 0.363 ± 0.098
0.824CysLeu: 0.824 ± 0.115
0.194CysMet: 0.194 ± 0.074
0.315CysAsn: 0.315 ± 0.098
0.582CysPro: 0.582 ± 0.141
0.388CysGln: 0.388 ± 0.116
0.848CysArg: 0.848 ± 0.167
0.703CysSer: 0.703 ± 0.187
0.412CysThr: 0.412 ± 0.108
0.897CysVal: 0.897 ± 0.165
0.218CysTrp: 0.218 ± 0.073
0.412CysTyr: 0.412 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
3.15AspAla: 3.15 ± 0.328
1.139AspCys: 1.139 ± 0.169
2.205AspAsp: 2.205 ± 0.225
3.029AspGlu: 3.029 ± 0.28
2.35AspPhe: 2.35 ± 0.259
5.282AspGly: 5.282 ± 0.372
1.163AspHis: 1.163 ± 0.204
2.568AspIle: 2.568 ± 0.303
2.472AspLys: 2.472 ± 0.263
5.84AspLeu: 5.84 ± 0.409
1.236AspMet: 1.236 ± 0.203
2.399AspAsn: 2.399 ± 0.23
4.168AspPro: 4.168 ± 0.326
1.672AspGln: 1.672 ± 0.164
2.835AspArg: 2.835 ± 0.247
5.161AspSer: 5.161 ± 0.415
2.787AspThr: 2.787 ± 0.261
2.375AspVal: 2.375 ± 0.239
1.26AspTrp: 1.26 ± 0.225
2.714AspTyr: 2.714 ± 0.283
0.0AspXaa: 0.0 ± 0.0
Glu
5.718GluAla: 5.718 ± 0.428
0.557GluCys: 0.557 ± 0.114
4.677GluAsp: 4.677 ± 0.361
7.487GluGlu: 7.487 ± 0.834
3.901GluPhe: 3.901 ± 0.334
5.088GluGly: 5.088 ± 0.414
1.018GluHis: 1.018 ± 0.163
5.088GluIle: 5.088 ± 0.396
4.24GluLys: 4.24 ± 0.422
6.13GluLeu: 6.13 ± 0.39
1.842GluMet: 1.842 ± 0.27
3.392GluAsn: 3.392 ± 0.241
3.029GluPro: 3.029 ± 0.321
1.914GluGln: 1.914 ± 0.23
4.265GluArg: 4.265 ± 0.388
4.362GluSer: 4.362 ± 0.33
3.586GluThr: 3.586 ± 0.307
5.743GluVal: 5.743 ± 0.394
1.502GluTrp: 1.502 ± 0.217
2.011GluTyr: 2.011 ± 0.212
0.0GluXaa: 0.0 ± 0.0
Phe
2.278PheAla: 2.278 ± 0.211
0.557PheCys: 0.557 ± 0.117
2.35PheAsp: 2.35 ± 0.233
2.35PheGlu: 2.35 ± 0.243
2.205PhePhe: 2.205 ± 0.217
2.859PheGly: 2.859 ± 0.282
0.751PheHis: 0.751 ± 0.148
2.06PheIle: 2.06 ± 0.224
1.551PheLys: 1.551 ± 0.218
4.289PheLeu: 4.289 ± 0.368
0.897PheMet: 0.897 ± 0.163
1.866PheAsn: 1.866 ± 0.235
2.568PhePro: 2.568 ± 0.285
1.212PheGln: 1.212 ± 0.158
2.714PheArg: 2.714 ± 0.295
4.071PheSer: 4.071 ± 0.336
2.641PheThr: 2.641 ± 0.234
2.714PheVal: 2.714 ± 0.328
0.63PheTrp: 0.63 ± 0.127
1.696PheTyr: 1.696 ± 0.165
0.0PheXaa: 0.0 ± 0.0
Gly
6.009GlyAla: 6.009 ± 0.51
0.848GlyCys: 0.848 ± 0.185
5.379GlyAsp: 5.379 ± 0.368
6.373GlyGlu: 6.373 ± 0.533
3.271GlyPhe: 3.271 ± 0.261
6.736GlyGly: 6.736 ± 0.473
1.163GlyHis: 1.163 ± 0.185
4.604GlyIle: 4.604 ± 0.392
4.846GlyLys: 4.846 ± 0.458
5.646GlyLeu: 5.646 ± 0.42
1.866GlyMet: 1.866 ± 0.21
3.804GlyAsn: 3.804 ± 0.317
3.853GlyPro: 3.853 ± 0.918
1.914GlyGln: 1.914 ± 0.191
4.555GlyArg: 4.555 ± 0.307
5.767GlySer: 5.767 ± 0.405
5.016GlyThr: 5.016 ± 0.436
6.397GlyVal: 6.397 ± 0.44
1.09GlyTrp: 1.09 ± 0.178
2.375GlyTyr: 2.375 ± 0.244
0.0GlyXaa: 0.0 ± 0.0
His
0.921HisAla: 0.921 ± 0.157
0.121HisCys: 0.121 ± 0.048
0.485HisAsp: 0.485 ± 0.106
0.727HisGlu: 0.727 ± 0.126
0.727HisPhe: 0.727 ± 0.137
1.212HisGly: 1.212 ± 0.162
0.291HisHis: 0.291 ± 0.08
0.897HisIle: 0.897 ± 0.135
0.945HisLys: 0.945 ± 0.17
1.236HisLeu: 1.236 ± 0.172
0.242HisMet: 0.242 ± 0.075
0.63HisAsn: 0.63 ± 0.157
1.09HisPro: 1.09 ± 0.171
0.582HisGln: 0.582 ± 0.107
1.115HisArg: 1.115 ± 0.19
1.236HisSer: 1.236 ± 0.201
0.775HisThr: 0.775 ± 0.145
0.897HisVal: 0.897 ± 0.151
0.315HisTrp: 0.315 ± 0.091
0.824HisTyr: 0.824 ± 0.154
0.0HisXaa: 0.0 ± 0.0
Ile
4.095IleAla: 4.095 ± 0.329
0.533IleCys: 0.533 ± 0.126
2.787IleAsp: 2.787 ± 0.268
3.174IleGlu: 3.174 ± 0.327
2.181IlePhe: 2.181 ± 0.258
4.628IleGly: 4.628 ± 0.343
1.09IleHis: 1.09 ± 0.165
2.084IleIle: 2.084 ± 0.243
2.084IleLys: 2.084 ± 0.208
4.798IleLeu: 4.798 ± 0.387
1.018IleMet: 1.018 ± 0.162
2.229IleAsn: 2.229 ± 0.226
3.756IlePro: 3.756 ± 0.316
2.108IleGln: 2.108 ± 0.223
3.998IleArg: 3.998 ± 0.353
5.137IleSer: 5.137 ± 0.334
3.077IleThr: 3.077 ± 0.288
3.756IleVal: 3.756 ± 0.276
0.872IleTrp: 0.872 ± 0.151
1.817IleTyr: 1.817 ± 0.183
0.0IleXaa: 0.0 ± 0.0
Lys
3.441LysAla: 3.441 ± 0.431
0.291LysCys: 0.291 ± 0.082
3.368LysAsp: 3.368 ± 0.361
4.434LysGlu: 4.434 ± 0.413
1.817LysPhe: 1.817 ± 0.217
3.683LysGly: 3.683 ± 0.326
0.557LysHis: 0.557 ± 0.117
3.659LysIle: 3.659 ± 0.312
3.586LysLys: 3.586 ± 0.434
3.925LysLeu: 3.925 ± 0.392
1.308LysMet: 1.308 ± 0.195
1.866LysAsn: 1.866 ± 0.238
1.72LysPro: 1.72 ± 0.204
1.333LysGln: 1.333 ± 0.233
2.278LysArg: 2.278 ± 0.278
2.956LysSer: 2.956 ± 0.311
3.053LysThr: 3.053 ± 0.266
4.047LysVal: 4.047 ± 0.389
0.606LysTrp: 0.606 ± 0.11
1.745LysTyr: 1.745 ± 0.219
0.0LysXaa: 0.0 ± 0.0
Leu
6.276LeuAla: 6.276 ± 0.424
0.969LeuCys: 0.969 ± 0.16
4.846LeuAsp: 4.846 ± 0.32
5.573LeuGlu: 5.573 ± 0.426
3.247LeuPhe: 3.247 ± 0.318
6.615LeuGly: 6.615 ± 0.438
1.381LeuHis: 1.381 ± 0.217
4.458LeuIle: 4.458 ± 0.315
3.853LeuLys: 3.853 ± 0.298
6.47LeuLeu: 6.47 ± 0.557
1.817LeuMet: 1.817 ± 0.196
3.562LeuAsn: 3.562 ± 0.283
4.967LeuPro: 4.967 ± 0.502
2.496LeuGln: 2.496 ± 0.275
4.798LeuArg: 4.798 ± 0.385
7.754LeuSer: 7.754 ± 0.514
5.282LeuThr: 5.282 ± 0.371
6.082LeuVal: 6.082 ± 0.462
1.187LeuTrp: 1.187 ± 0.143
2.908LeuTyr: 2.908 ± 0.296
0.0LeuXaa: 0.0 ± 0.0
Met
2.229MetAla: 2.229 ± 0.261
0.121MetCys: 0.121 ± 0.05
1.236MetAsp: 1.236 ± 0.158
1.769MetGlu: 1.769 ± 0.217
0.872MetPhe: 0.872 ± 0.165
1.405MetGly: 1.405 ± 0.209
0.242MetHis: 0.242 ± 0.109
1.308MetIle: 1.308 ± 0.19
1.042MetLys: 1.042 ± 0.168
1.405MetLeu: 1.405 ± 0.214
0.412MetMet: 0.412 ± 0.102
1.163MetAsn: 1.163 ± 0.165
1.066MetPro: 1.066 ± 0.161
0.727MetGln: 0.727 ± 0.145
1.43MetArg: 1.43 ± 0.213
1.478MetSer: 1.478 ± 0.177
1.43MetThr: 1.43 ± 0.202
1.284MetVal: 1.284 ± 0.185
0.291MetTrp: 0.291 ± 0.073
0.412MetTyr: 0.412 ± 0.107
0.0MetXaa: 0.0 ± 0.0
Asn
3.174AsnAla: 3.174 ± 0.33
0.533AsnCys: 0.533 ± 0.119
1.914AsnAsp: 1.914 ± 0.189
2.229AsnGlu: 2.229 ± 0.245
2.496AsnPhe: 2.496 ± 0.232
3.732AsnGly: 3.732 ± 0.349
0.703AsnHis: 0.703 ± 0.148
1.914AsnIle: 1.914 ± 0.207
2.157AsnLys: 2.157 ± 0.301
4.386AsnLeu: 4.386 ± 0.345
0.582AsnMet: 0.582 ± 0.126
2.181AsnAsn: 2.181 ± 0.236
2.714AsnPro: 2.714 ± 0.239
1.527AsnGln: 1.527 ± 0.196
2.035AsnArg: 2.035 ± 0.229
3.877AsnSer: 3.877 ± 0.355
1.987AsnThr: 1.987 ± 0.244
2.544AsnVal: 2.544 ± 0.313
0.848AsnTrp: 0.848 ± 0.146
1.793AsnTyr: 1.793 ± 0.176
0.0AsnXaa: 0.0 ± 0.0
Pro
3.95ProAla: 3.95 ± 0.411
0.291ProCys: 0.291 ± 0.078
3.198ProAsp: 3.198 ± 0.328
5.21ProGlu: 5.21 ± 0.377
2.132ProPhe: 2.132 ± 0.214
3.562ProGly: 3.562 ± 0.425
0.848ProHis: 0.848 ± 0.159
2.762ProIle: 2.762 ± 0.26
2.302ProLys: 2.302 ± 0.265
3.756ProLeu: 3.756 ± 0.302
0.897ProMet: 0.897 ± 0.176
2.229ProAsn: 2.229 ± 0.286
2.399ProPro: 2.399 ± 0.262
1.769ProGln: 1.769 ± 0.247
1.987ProArg: 1.987 ± 0.249
4.507ProSer: 4.507 ± 0.391
3.344ProThr: 3.344 ± 0.417
3.756ProVal: 3.756 ± 0.333
0.654ProTrp: 0.654 ± 0.12
1.599ProTyr: 1.599 ± 0.178
0.0ProXaa: 0.0 ± 0.0
Gln
2.641GlnAla: 2.641 ± 0.278
0.218GlnCys: 0.218 ± 0.077
1.648GlnAsp: 1.648 ± 0.187
2.738GlnGlu: 2.738 ± 0.254
1.236GlnPhe: 1.236 ± 0.154
3.198GlnGly: 3.198 ± 0.515
0.533GlnHis: 0.533 ± 0.112
2.181GlnIle: 2.181 ± 0.249
1.527GlnLys: 1.527 ± 0.242
2.326GlnLeu: 2.326 ± 0.258
0.678GlnMet: 0.678 ± 0.148
1.066GlnAsn: 1.066 ± 0.149
1.139GlnPro: 1.139 ± 0.171
0.872GlnGln: 0.872 ± 0.183
1.672GlnArg: 1.672 ± 0.198
1.89GlnSer: 1.89 ± 0.232
1.696GlnThr: 1.696 ± 0.224
2.278GlnVal: 2.278 ± 0.265
0.363GlnTrp: 0.363 ± 0.08
0.872GlnTyr: 0.872 ± 0.143
0.0GlnXaa: 0.0 ± 0.0
Arg
4.216ArgAla: 4.216 ± 0.362
0.824ArgCys: 0.824 ± 0.177
2.883ArgAsp: 2.883 ± 0.296
4.846ArgGlu: 4.846 ± 0.388
2.641ArgPhe: 2.641 ± 0.212
4.095ArgGly: 4.095 ± 0.325
0.582ArgHis: 0.582 ± 0.093
3.586ArgIle: 3.586 ± 0.31
3.198ArgLys: 3.198 ± 0.32
4.628ArgLeu: 4.628 ± 0.365
1.308ArgMet: 1.308 ± 0.191
2.908ArgAsn: 2.908 ± 0.271
2.035ArgPro: 2.035 ± 0.266
1.308ArgGln: 1.308 ± 0.167
3.271ArgArg: 3.271 ± 0.333
3.465ArgSer: 3.465 ± 0.341
2.835ArgThr: 2.835 ± 0.249
4.362ArgVal: 4.362 ± 0.286
0.848ArgTrp: 0.848 ± 0.154
1.769ArgTyr: 1.769 ± 0.189
0.0ArgXaa: 0.0 ± 0.0
Ser
5.791SerAla: 5.791 ± 0.367
0.63SerCys: 0.63 ± 0.135
4.168SerAsp: 4.168 ± 0.383
5.767SerGlu: 5.767 ± 0.464
3.077SerPhe: 3.077 ± 0.304
7.318SerGly: 7.318 ± 0.474
1.139SerHis: 1.139 ± 0.171
3.828SerIle: 3.828 ± 0.346
2.447SerLys: 2.447 ± 0.265
7.366SerLeu: 7.366 ± 0.513
1.454SerMet: 1.454 ± 0.185
2.641SerAsn: 2.641 ± 0.246
4.095SerPro: 4.095 ± 0.288
3.126SerGln: 3.126 ± 0.285
4.337SerArg: 4.337 ± 0.376
5.961SerSer: 5.961 ± 0.492
4.87SerThr: 4.87 ± 0.418
4.725SerVal: 4.725 ± 0.366
1.357SerTrp: 1.357 ± 0.204
3.126SerTyr: 3.126 ± 0.365
0.0SerXaa: 0.0 ± 0.0
Thr
4.701ThrAla: 4.701 ± 0.448
0.412ThrCys: 0.412 ± 0.099
2.883ThrAsp: 2.883 ± 0.267
3.707ThrGlu: 3.707 ± 0.3
2.399ThrPhe: 2.399 ± 0.244
6.058ThrGly: 6.058 ± 0.809
0.703ThrHis: 0.703 ± 0.142
3.32ThrIle: 3.32 ± 0.353
2.302ThrLys: 2.302 ± 0.3
5.307ThrLeu: 5.307 ± 0.32
1.09ThrMet: 1.09 ± 0.148
2.593ThrAsn: 2.593 ± 0.262
3.513ThrPro: 3.513 ± 0.292
1.284ThrGln: 1.284 ± 0.172
2.762ThrArg: 2.762 ± 0.241
4.725ThrSer: 4.725 ± 0.486
3.392ThrThr: 3.392 ± 0.332
3.78ThrVal: 3.78 ± 0.335
0.751ThrTrp: 0.751 ± 0.158
1.866ThrTyr: 1.866 ± 0.19
0.0ThrXaa: 0.0 ± 0.0
Val
5.549ValAla: 5.549 ± 0.358
0.824ValCys: 0.824 ± 0.135
3.974ValAsp: 3.974 ± 0.28
5.379ValGlu: 5.379 ± 0.433
2.932ValPhe: 2.932 ± 0.277
5.331ValGly: 5.331 ± 0.445
0.775ValHis: 0.775 ± 0.136
3.659ValIle: 3.659 ± 0.247
3.828ValLys: 3.828 ± 0.301
4.725ValLeu: 4.725 ± 0.285
1.26ValMet: 1.26 ± 0.184
2.762ValAsn: 2.762 ± 0.273
3.465ValPro: 3.465 ± 0.389
2.229ValGln: 2.229 ± 0.334
4.337ValArg: 4.337 ± 0.354
5.016ValSer: 5.016 ± 0.325
3.877ValThr: 3.877 ± 0.369
4.58ValVal: 4.58 ± 0.478
0.993ValTrp: 0.993 ± 0.171
2.253ValTyr: 2.253 ± 0.263
0.0ValXaa: 0.0 ± 0.0
Trp
1.066TrpAla: 1.066 ± 0.155
0.17TrpCys: 0.17 ± 0.073
0.897TrpAsp: 0.897 ± 0.174
1.623TrpGlu: 1.623 ± 0.196
0.412TrpPhe: 0.412 ± 0.103
1.066TrpGly: 1.066 ± 0.19
0.194TrpHis: 0.194 ± 0.059
0.678TrpIle: 0.678 ± 0.129
1.018TrpLys: 1.018 ± 0.139
1.333TrpLeu: 1.333 ± 0.183
0.509TrpMet: 0.509 ± 0.116
0.8TrpAsn: 0.8 ± 0.132
0.363TrpPro: 0.363 ± 0.089
0.509TrpGln: 0.509 ± 0.124
0.8TrpArg: 0.8 ± 0.126
1.066TrpSer: 1.066 ± 0.195
0.8TrpThr: 0.8 ± 0.165
1.381TrpVal: 1.381 ± 0.191
0.315TrpTrp: 0.315 ± 0.073
0.509TrpTyr: 0.509 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.714TyrAla: 2.714 ± 0.228
0.436TyrCys: 0.436 ± 0.093
2.35TyrAsp: 2.35 ± 0.237
2.447TyrGlu: 2.447 ± 0.229
1.09TyrPhe: 1.09 ± 0.137
2.738TyrGly: 2.738 ± 0.26
0.63TyrHis: 0.63 ± 0.114
1.284TyrIle: 1.284 ± 0.166
1.139TyrLys: 1.139 ± 0.166
3.174TyrLeu: 3.174 ± 0.273
0.485TyrMet: 0.485 ± 0.097
1.43TyrAsn: 1.43 ± 0.185
1.648TyrPro: 1.648 ± 0.244
1.43TyrGln: 1.43 ± 0.2
2.278TyrArg: 2.278 ± 0.271
3.077TyrSer: 3.077 ± 0.308
1.89TyrThr: 1.89 ± 0.239
2.399TyrVal: 2.399 ± 0.313
0.509TyrTrp: 0.509 ± 0.1
0.897TyrTyr: 0.897 ± 0.172
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 178 proteins (41271 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski