Synchytrium microbalum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Chytridiomycota; Chytridiomycota incertae sedis; Chytridiomycetes; Synchytriales; Synchytriaceae; Synchytrium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6304 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A507CG55|A0A507CG55_9FUNG N-acetyltransferase domain-containing protein OS=Synchytrium microbalum OX=1806994 GN=SmJEL517_g00174 PE=4 SV=1
MM1 pKa = 7.33TSFYY5 pKa = 11.22DD6 pKa = 3.49EE7 pKa = 4.79VEE9 pKa = 4.17IEE11 pKa = 3.93DD12 pKa = 4.56LEE14 pKa = 4.62FNEE17 pKa = 4.09EE18 pKa = 3.95TRR20 pKa = 11.84IYY22 pKa = 10.08TYY24 pKa = 10.36PCPCGDD30 pKa = 3.32KK31 pKa = 10.71FQISEE36 pKa = 3.93EE37 pKa = 4.48DD38 pKa = 3.22IFDD41 pKa = 4.18GEE43 pKa = 4.8DD44 pKa = 2.92IARR47 pKa = 11.84CPSCSLLIRR56 pKa = 11.84VIYY59 pKa = 10.21DD60 pKa = 3.06PSDD63 pKa = 3.29FEE65 pKa = 4.77RR66 pKa = 11.84PGDD69 pKa = 3.66EE70 pKa = 4.01GGEE73 pKa = 4.26TIEE76 pKa = 4.21IKK78 pKa = 10.79QPIAVV83 pKa = 3.7

Molecular weight: 9.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A507CDX5|A0A507CDX5_9FUNG Uncharacterized protein OS=Synchytrium microbalum OX=1806994 GN=SmJEL517_g00534 PE=4 SV=1
PP1 pKa = 6.98SNKK4 pKa = 8.17TFRR7 pKa = 11.84IKK9 pKa = 10.6RR10 pKa = 11.84ILGKK14 pKa = 9.65KK15 pKa = 8.74QKK17 pKa = 9.04QNRR20 pKa = 11.84PIPNWIRR27 pKa = 11.84LRR29 pKa = 11.84TNNTIRR35 pKa = 11.84YY36 pKa = 7.29NSKK39 pKa = 8.9RR40 pKa = 11.84RR41 pKa = 11.84NWRR44 pKa = 11.84RR45 pKa = 11.84TKK47 pKa = 11.05LNLL50 pKa = 3.44

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6304

0

6304

3623650

11

4812

574.8

63.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.351 ± 0.028

1.163 ± 0.011

5.764 ± 0.024

5.907 ± 0.028

3.644 ± 0.019

6.15 ± 0.023

2.226 ± 0.012

5.422 ± 0.018

5.418 ± 0.024

9.13 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.403 ± 0.011

4.055 ± 0.016

5.541 ± 0.026

3.99 ± 0.023

5.482 ± 0.022

8.44 ± 0.032

6.165 ± 0.018

6.731 ± 0.019

1.181 ± 0.01

2.839 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski