Coprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / OCM 4 / BT)

Taxonomy: cellular organisms; Bacteria; Coprothermobacterota; Coprothermobacteria; Coprothermobacterales; Coprothermobacteraceae; Coprothermobacter; Coprothermobacter proteolyticus

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1481 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B5Y8L4|B5Y8L4_COPPD Aspartate--tRNA ligase OS=Coprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / OCM 4 / BT) OX=309798 GN=aspS PE=3 SV=1
MM1 pKa = 7.31AVKK4 pKa = 10.11VDD6 pKa = 3.73KK7 pKa = 10.41DD8 pKa = 3.46LCIACGVCMALCPEE22 pKa = 4.64VFQADD27 pKa = 3.62AEE29 pKa = 4.69GKK31 pKa = 10.07SEE33 pKa = 4.45VIPGADD39 pKa = 3.04EE40 pKa = 4.45SLPCVDD46 pKa = 5.06EE47 pKa = 6.22AIDD50 pKa = 3.79SCSTGAISRR59 pKa = 11.84EE60 pKa = 4.04

Molecular weight: 6.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5Y6Q5|B5Y6Q5_COPPD Protease OS=Coprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / OCM 4 / BT) OX=309798 GN=COPRO5265_0081 PE=3 SV=1
MM1 pKa = 7.1LQITAEE7 pKa = 4.69LIWRR11 pKa = 11.84TLVFTVATTALACVMALLVGTIYY34 pKa = 11.05ASLVRR39 pKa = 11.84SPWSHH44 pKa = 6.28LSFWRR49 pKa = 11.84AMSSVPPVLIGLLVYY64 pKa = 10.83LLFSRR69 pKa = 11.84QGPLGFLRR77 pKa = 11.84WLFTPKK83 pKa = 10.67ALVVAQFLLALPMAISLCLQHH104 pKa = 6.87RR105 pKa = 11.84VSLPRR110 pKa = 11.84DD111 pKa = 3.34MEE113 pKa = 4.01EE114 pKa = 4.65FIDD117 pKa = 4.09VNLPKK122 pKa = 10.29KK123 pKa = 10.32RR124 pKa = 11.84LNAMLLHH131 pKa = 6.17LRR133 pKa = 11.84GGLVNIGFLTWGRR146 pKa = 11.84IMGEE150 pKa = 3.82VGAATLSGGAIVGYY164 pKa = 9.06TSTLSTEE171 pKa = 3.3IMYY174 pKa = 7.79RR175 pKa = 11.84TNLGQFGNAIALGIVLLLISLIPSVILEE203 pKa = 4.25VTSRR207 pKa = 11.84AQRR210 pKa = 3.34

Molecular weight: 23.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1481

0

1481

439573

30

2037

296.8

33.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.642 ± 0.066

0.783 ± 0.02

5.056 ± 0.051

7.133 ± 0.083

4.367 ± 0.044

7.265 ± 0.056

1.694 ± 0.028

6.308 ± 0.048

5.976 ± 0.056

10.692 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.588 ± 0.03

3.697 ± 0.035

4.316 ± 0.034

3.266 ± 0.037

4.644 ± 0.049

6.113 ± 0.058

5.209 ± 0.06

8.665 ± 0.054

1.197 ± 0.023

3.388 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski