Amino acid dipepetide frequency for Coprothermobacter proteolyticus (strain ATCC 35245 / DSM 5265 / OCM 4 / BT)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.279AlaAla: 6.279 ± 0.129
0.642AlaCys: 0.642 ± 0.042
3.649AlaAsp: 3.649 ± 0.082
5.071AlaGlu: 5.071 ± 0.108
3.497AlaPhe: 3.497 ± 0.097
5.383AlaGly: 5.383 ± 0.132
1.354AlaHis: 1.354 ± 0.055
4.907AlaIle: 4.907 ± 0.113
4.434AlaLys: 4.434 ± 0.102
9.477AlaLeu: 9.477 ± 0.161
2.305AlaMet: 2.305 ± 0.07
2.487AlaAsn: 2.487 ± 0.076
2.477AlaPro: 2.477 ± 0.084
2.623AlaGln: 2.623 ± 0.085
3.253AlaArg: 3.253 ± 0.081
4.357AlaSer: 4.357 ± 0.109
3.872AlaThr: 3.872 ± 0.097
6.977AlaVal: 6.977 ± 0.14
0.71AlaTrp: 0.71 ± 0.04
2.664AlaTyr: 2.664 ± 0.088
0.0AlaXaa: 0.0 ± 0.0
Cys
0.539CysAla: 0.539 ± 0.037
0.084CysCys: 0.084 ± 0.016
0.423CysAsp: 0.423 ± 0.03
0.409CysGlu: 0.409 ± 0.028
0.35CysPhe: 0.35 ± 0.025
0.764CysGly: 0.764 ± 0.044
0.18CysHis: 0.18 ± 0.02
0.425CysIle: 0.425 ± 0.034
0.425CysLys: 0.425 ± 0.03
0.719CysLeu: 0.719 ± 0.04
0.218CysMet: 0.218 ± 0.022
0.278CysAsn: 0.278 ± 0.025
0.514CysPro: 0.514 ± 0.04
0.221CysGln: 0.221 ± 0.021
0.318CysArg: 0.318 ± 0.026
0.56CysSer: 0.56 ± 0.037
0.432CysThr: 0.432 ± 0.036
0.582CysVal: 0.582 ± 0.043
0.116CysTrp: 0.116 ± 0.017
0.273CysTyr: 0.273 ± 0.025
0.0CysXaa: 0.0 ± 0.0
Asp
3.772AspAla: 3.772 ± 0.088
0.307AspCys: 0.307 ± 0.026
2.282AspAsp: 2.282 ± 0.076
3.731AspGlu: 3.731 ± 0.104
2.634AspPhe: 2.634 ± 0.079
3.34AspGly: 3.34 ± 0.102
0.849AspHis: 0.849 ± 0.038
3.64AspIle: 3.64 ± 0.089
2.848AspLys: 2.848 ± 0.076
5.571AspLeu: 5.571 ± 0.139
1.365AspMet: 1.365 ± 0.042
1.599AspAsn: 1.599 ± 0.061
2.698AspPro: 2.698 ± 0.084
1.47AspGln: 1.47 ± 0.058
2.141AspArg: 2.141 ± 0.082
2.427AspSer: 2.427 ± 0.066
2.204AspThr: 2.204 ± 0.08
4.9AspVal: 4.9 ± 0.117
0.703AspTrp: 0.703 ± 0.045
2.079AspTyr: 2.079 ± 0.065
0.0AspXaa: 0.0 ± 0.0
Glu
5.48GluAla: 5.48 ± 0.109
0.357GluCys: 0.357 ± 0.027
3.553GluAsp: 3.553 ± 0.093
7.055GluGlu: 7.055 ± 0.167
2.334GluPhe: 2.334 ± 0.075
4.816GluGly: 4.816 ± 0.115
1.294GluHis: 1.294 ± 0.053
4.491GluIle: 4.491 ± 0.125
5.314GluLys: 5.314 ± 0.138
7.344GluLeu: 7.344 ± 0.147
1.806GluMet: 1.806 ± 0.067
3.133GluAsn: 3.133 ± 0.087
2.6GluPro: 2.6 ± 0.089
2.914GluGln: 2.914 ± 0.088
3.79GluArg: 3.79 ± 0.098
3.399GluSer: 3.399 ± 0.093
3.135GluThr: 3.135 ± 0.096
5.935GluVal: 5.935 ± 0.118
0.61GluTrp: 0.61 ± 0.039
1.966GluTyr: 1.966 ± 0.08
0.0GluXaa: 0.0 ± 0.0
Phe
3.187PheAla: 3.187 ± 0.096
0.396PheCys: 0.396 ± 0.03
2.366PheAsp: 2.366 ± 0.08
2.421PheGlu: 2.421 ± 0.08
2.173PhePhe: 2.173 ± 0.079
3.526PheGly: 3.526 ± 0.081
0.68PheHis: 0.68 ± 0.043
2.803PheIle: 2.803 ± 0.091
2.118PheLys: 2.118 ± 0.068
4.991PheLeu: 4.991 ± 0.115
1.183PheMet: 1.183 ± 0.054
1.451PheAsn: 1.451 ± 0.064
1.761PhePro: 1.761 ± 0.071
1.347PheGln: 1.347 ± 0.055
1.722PheArg: 1.722 ± 0.063
3.201PheSer: 3.201 ± 0.104
2.396PheThr: 2.396 ± 0.073
3.669PheVal: 3.669 ± 0.088
0.61PheTrp: 0.61 ± 0.042
1.674PheTyr: 1.674 ± 0.063
0.0PheXaa: 0.0 ± 0.0
Gly
5.476GlyAla: 5.476 ± 0.118
0.676GlyCys: 0.676 ± 0.05
3.319GlyAsp: 3.319 ± 0.089
4.477GlyGlu: 4.477 ± 0.106
3.617GlyPhe: 3.617 ± 0.105
4.95GlyGly: 4.95 ± 0.144
1.326GlyHis: 1.326 ± 0.058
5.346GlyIle: 5.346 ± 0.123
5.0GlyLys: 5.0 ± 0.099
7.503GlyLeu: 7.503 ± 0.146
1.925GlyMet: 1.925 ± 0.076
2.662GlyAsn: 2.662 ± 0.074
2.289GlyPro: 2.289 ± 0.082
2.229GlyGln: 2.229 ± 0.074
3.189GlyArg: 3.189 ± 0.1
4.495GlySer: 4.495 ± 0.112
4.393GlyThr: 4.393 ± 0.116
6.056GlyVal: 6.056 ± 0.121
0.86GlyTrp: 0.86 ± 0.054
2.866GlyTyr: 2.866 ± 0.09
0.0GlyXaa: 0.0 ± 0.0
His
1.188HisAla: 1.188 ± 0.057
0.182HisCys: 0.182 ± 0.017
0.773HisAsp: 0.773 ± 0.039
1.128HisGlu: 1.128 ± 0.057
0.773HisPhe: 0.773 ± 0.04
1.426HisGly: 1.426 ± 0.057
0.355HisHis: 0.355 ± 0.032
1.174HisIle: 1.174 ± 0.053
0.771HisLys: 0.771 ± 0.041
1.875HisLeu: 1.875 ± 0.073
0.444HisMet: 0.444 ± 0.032
0.573HisAsn: 0.573 ± 0.035
0.949HisPro: 0.949 ± 0.042
0.432HisGln: 0.432 ± 0.03
0.805HisArg: 0.805 ± 0.042
0.912HisSer: 0.912 ± 0.05
0.853HisThr: 0.853 ± 0.046
1.474HisVal: 1.474 ± 0.058
0.257HisTrp: 0.257 ± 0.024
0.594HisTyr: 0.594 ± 0.036
0.0HisXaa: 0.0 ± 0.0
Ile
5.18IleAla: 5.18 ± 0.116
0.539IleCys: 0.539 ± 0.036
3.385IleAsp: 3.385 ± 0.081
4.154IleGlu: 4.154 ± 0.108
2.293IlePhe: 2.293 ± 0.073
4.659IleGly: 4.659 ± 0.127
0.958IleHis: 0.958 ± 0.041
3.854IleIle: 3.854 ± 0.111
3.902IleLys: 3.902 ± 0.097
6.356IleLeu: 6.356 ± 0.142
1.531IleMet: 1.531 ± 0.062
2.398IleAsn: 2.398 ± 0.072
3.246IlePro: 3.246 ± 0.094
1.583IleGln: 1.583 ± 0.056
3.087IleArg: 3.087 ± 0.091
4.266IleSer: 4.266 ± 0.103
3.933IleThr: 3.933 ± 0.101
5.148IleVal: 5.148 ± 0.116
0.651IleTrp: 0.651 ± 0.043
1.954IleTyr: 1.954 ± 0.062
0.0IleXaa: 0.0 ± 0.0
Lys
4.968LysAla: 4.968 ± 0.103
0.373LysCys: 0.373 ± 0.031
3.394LysAsp: 3.394 ± 0.08
5.474LysGlu: 5.474 ± 0.12
1.79LysPhe: 1.79 ± 0.071
4.304LysGly: 4.304 ± 0.104
0.987LysHis: 0.987 ± 0.049
3.069LysIle: 3.069 ± 0.095
3.792LysLys: 3.792 ± 0.094
5.578LysLeu: 5.578 ± 0.124
1.347LysMet: 1.347 ± 0.056
2.618LysAsn: 2.618 ± 0.087
2.689LysPro: 2.689 ± 0.083
2.182LysGln: 2.182 ± 0.073
2.985LysArg: 2.985 ± 0.088
3.196LysSer: 3.196 ± 0.095
3.237LysThr: 3.237 ± 0.09
5.1LysVal: 5.1 ± 0.116
0.705LysTrp: 0.705 ± 0.04
1.972LysTyr: 1.972 ± 0.063
0.0LysXaa: 0.0 ± 0.0
Leu
8.135LeuAla: 8.135 ± 0.16
0.862LeuCys: 0.862 ± 0.044
5.448LeuAsp: 5.448 ± 0.105
7.516LeuGlu: 7.516 ± 0.151
4.179LeuPhe: 4.179 ± 0.121
7.98LeuGly: 7.98 ± 0.151
1.656LeuHis: 1.656 ± 0.062
6.22LeuIle: 6.22 ± 0.15
6.509LeuLys: 6.509 ± 0.122
11.322LeuLeu: 11.322 ± 0.228
2.753LeuMet: 2.753 ± 0.082
3.817LeuAsn: 3.817 ± 0.093
5.091LeuPro: 5.091 ± 0.116
3.613LeuGln: 3.613 ± 0.094
5.342LeuArg: 5.342 ± 0.123
8.037LeuSer: 8.037 ± 0.161
5.471LeuThr: 5.471 ± 0.124
8.583LeuVal: 8.583 ± 0.169
1.299LeuTrp: 1.299 ± 0.063
3.089LeuTyr: 3.089 ± 0.092
0.0LeuXaa: 0.0 ± 0.0
Met
2.364MetAla: 2.364 ± 0.074
0.196MetCys: 0.196 ± 0.024
1.458MetAsp: 1.458 ± 0.054
1.97MetGlu: 1.97 ± 0.064
0.933MetPhe: 0.933 ± 0.045
2.107MetGly: 2.107 ± 0.07
0.407MetHis: 0.407 ± 0.031
1.317MetIle: 1.317 ± 0.058
1.727MetLys: 1.727 ± 0.059
2.487MetLeu: 2.487 ± 0.077
0.587MetMet: 0.587 ± 0.035
1.081MetAsn: 1.081 ± 0.047
1.267MetPro: 1.267 ± 0.053
0.921MetGln: 0.921 ± 0.047
1.183MetArg: 1.183 ± 0.051
1.588MetSer: 1.588 ± 0.065
1.135MetThr: 1.135 ± 0.047
2.248MetVal: 2.248 ± 0.075
0.289MetTrp: 0.289 ± 0.025
0.612MetTyr: 0.612 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
2.889AsnAla: 2.889 ± 0.085
0.296AsnCys: 0.296 ± 0.027
1.631AsnAsp: 1.631 ± 0.067
2.15AsnGlu: 2.15 ± 0.081
1.474AsnPhe: 1.474 ± 0.066
2.691AsnGly: 2.691 ± 0.081
0.61AsnHis: 0.61 ± 0.039
2.666AsnIle: 2.666 ± 0.079
1.781AsnLys: 1.781 ± 0.073
4.115AsnLeu: 4.115 ± 0.095
1.099AsnMet: 1.099 ± 0.048
1.383AsnAsn: 1.383 ± 0.068
2.168AsnPro: 2.168 ± 0.069
1.144AsnGln: 1.144 ± 0.052
1.595AsnArg: 1.595 ± 0.066
2.054AsnSer: 2.054 ± 0.071
1.829AsnThr: 1.829 ± 0.061
3.519AsnVal: 3.519 ± 0.094
0.523AsnTrp: 0.523 ± 0.034
1.356AsnTyr: 1.356 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
2.762ProAla: 2.762 ± 0.088
0.307ProCys: 0.307 ± 0.032
2.277ProAsp: 2.277 ± 0.071
3.729ProGlu: 3.729 ± 0.102
2.175ProPhe: 2.175 ± 0.07
2.91ProGly: 2.91 ± 0.09
0.819ProHis: 0.819 ± 0.045
2.459ProIle: 2.459 ± 0.082
2.3ProLys: 2.3 ± 0.082
4.543ProLeu: 4.543 ± 0.107
1.181ProMet: 1.181 ± 0.048
1.613ProAsn: 1.613 ± 0.067
1.686ProPro: 1.686 ± 0.072
1.49ProGln: 1.49 ± 0.065
1.572ProArg: 1.572 ± 0.049
2.621ProSer: 2.621 ± 0.071
2.37ProThr: 2.37 ± 0.094
3.995ProVal: 3.995 ± 0.076
0.637ProTrp: 0.637 ± 0.04
1.713ProTyr: 1.713 ± 0.064
0.0ProXaa: 0.0 ± 0.0
Gln
2.334GlnAla: 2.334 ± 0.064
0.246GlnCys: 0.246 ± 0.023
1.435GlnAsp: 1.435 ± 0.058
2.546GlnGlu: 2.546 ± 0.075
1.213GlnPhe: 1.213 ± 0.048
2.32GlnGly: 2.32 ± 0.073
0.512GlnHis: 0.512 ± 0.032
2.179GlnIle: 2.179 ± 0.072
2.145GlnLys: 2.145 ± 0.061
3.501GlnLeu: 3.501 ± 0.092
0.944GlnMet: 0.944 ± 0.048
1.242GlnAsn: 1.242 ± 0.051
1.178GlnPro: 1.178 ± 0.053
1.397GlnGln: 1.397 ± 0.07
1.89GlnArg: 1.89 ± 0.068
1.743GlnSer: 1.743 ± 0.065
1.517GlnThr: 1.517 ± 0.056
2.814GlnVal: 2.814 ± 0.084
0.466GlnTrp: 0.466 ± 0.032
1.04GlnTyr: 1.04 ± 0.046
0.0GlnXaa: 0.0 ± 0.0
Arg
3.221ArgAla: 3.221 ± 0.082
0.344ArgCys: 0.344 ± 0.034
2.173ArgAsp: 2.173 ± 0.073
3.396ArgGlu: 3.396 ± 0.107
2.298ArgPhe: 2.298 ± 0.079
2.93ArgGly: 2.93 ± 0.087
0.819ArgHis: 0.819 ± 0.042
3.419ArgIle: 3.419 ± 0.097
2.989ArgLys: 2.989 ± 0.079
4.579ArgLeu: 4.579 ± 0.095
1.297ArgMet: 1.297 ± 0.054
1.888ArgAsn: 1.888 ± 0.07
1.724ArgPro: 1.724 ± 0.062
1.652ArgGln: 1.652 ± 0.052
2.709ArgArg: 2.709 ± 0.09
2.687ArgSer: 2.687 ± 0.086
2.38ArgThr: 2.38 ± 0.079
3.708ArgVal: 3.708 ± 0.108
0.635ArgTrp: 0.635 ± 0.039
1.588ArgTyr: 1.588 ± 0.066
0.0ArgXaa: 0.0 ± 0.0
Ser
4.502SerAla: 4.502 ± 0.108
0.528SerCys: 0.528 ± 0.033
2.964SerAsp: 2.964 ± 0.093
3.813SerGlu: 3.813 ± 0.095
3.203SerPhe: 3.203 ± 0.09
4.721SerGly: 4.721 ± 0.115
1.097SerHis: 1.097 ± 0.053
3.697SerIle: 3.697 ± 0.096
3.399SerLys: 3.399 ± 0.089
6.545SerLeu: 6.545 ± 0.156
1.67SerMet: 1.67 ± 0.061
2.118SerAsn: 2.118 ± 0.074
2.398SerPro: 2.398 ± 0.073
1.938SerGln: 1.938 ± 0.071
2.719SerArg: 2.719 ± 0.089
4.459SerSer: 4.459 ± 0.117
3.303SerThr: 3.303 ± 0.092
5.03SerVal: 5.03 ± 0.106
0.753SerTrp: 0.753 ± 0.047
2.279SerTyr: 2.279 ± 0.076
0.0SerXaa: 0.0 ± 0.0
Thr
4.188ThrAla: 4.188 ± 0.101
0.389ThrCys: 0.389 ± 0.035
2.605ThrAsp: 2.605 ± 0.079
2.973ThrGlu: 2.973 ± 0.101
2.43ThrPhe: 2.43 ± 0.075
4.291ThrGly: 4.291 ± 0.104
0.824ThrHis: 0.824 ± 0.051
3.199ThrIle: 3.199 ± 0.101
2.584ThrLys: 2.584 ± 0.077
6.01ThrLeu: 6.01 ± 0.126
1.338ThrMet: 1.338 ± 0.056
1.724ThrAsn: 1.724 ± 0.06
2.632ThrPro: 2.632 ± 0.086
1.556ThrGln: 1.556 ± 0.054
2.084ThrArg: 2.084 ± 0.07
3.139ThrSer: 3.139 ± 0.082
2.919ThrThr: 2.919 ± 0.093
4.921ThrVal: 4.921 ± 0.113
0.562ThrTrp: 0.562 ± 0.035
1.72ThrTyr: 1.72 ± 0.064
0.0ThrXaa: 0.0 ± 0.0
Val
6.586ValAla: 6.586 ± 0.123
0.712ValCys: 0.712 ± 0.046
4.866ValAsp: 4.866 ± 0.117
6.115ValGlu: 6.115 ± 0.137
4.033ValPhe: 4.033 ± 0.101
6.095ValGly: 6.095 ± 0.124
1.301ValHis: 1.301 ± 0.06
5.565ValIle: 5.565 ± 0.126
5.148ValLys: 5.148 ± 0.124
9.48ValLeu: 9.48 ± 0.147
1.925ValMet: 1.925 ± 0.069
3.149ValAsn: 3.149 ± 0.092
3.906ValPro: 3.906 ± 0.105
2.425ValGln: 2.425 ± 0.078
3.801ValArg: 3.801 ± 0.099
5.362ValSer: 5.362 ± 0.119
4.466ValThr: 4.466 ± 0.116
8.226ValVal: 8.226 ± 0.165
0.835ValTrp: 0.835 ± 0.049
2.657ValTyr: 2.657 ± 0.079
0.0ValXaa: 0.0 ± 0.0
Trp
0.833TrpAla: 0.833 ± 0.04
0.086TrpCys: 0.086 ± 0.016
0.726TrpAsp: 0.726 ± 0.042
0.826TrpGlu: 0.826 ± 0.049
0.587TrpPhe: 0.587 ± 0.041
0.817TrpGly: 0.817 ± 0.044
0.234TrpHis: 0.234 ± 0.023
0.792TrpIle: 0.792 ± 0.046
0.762TrpLys: 0.762 ± 0.044
1.358TrpLeu: 1.358 ± 0.061
0.23TrpMet: 0.23 ± 0.021
0.555TrpAsn: 0.555 ± 0.035
0.382TrpPro: 0.382 ± 0.031
0.494TrpGln: 0.494 ± 0.031
0.553TrpArg: 0.553 ± 0.034
0.737TrpSer: 0.737 ± 0.048
0.566TrpThr: 0.566 ± 0.035
0.783TrpVal: 0.783 ± 0.044
0.237TrpTrp: 0.237 ± 0.022
0.416TrpTyr: 0.416 ± 0.034
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.532TyrAla: 2.532 ± 0.079
0.312TyrCys: 0.312 ± 0.024
1.831TyrAsp: 1.831 ± 0.064
2.373TyrGlu: 2.373 ± 0.082
1.688TyrPhe: 1.688 ± 0.066
2.625TyrGly: 2.625 ± 0.081
0.678TyrHis: 0.678 ± 0.037
1.856TyrIle: 1.856 ± 0.069
1.815TyrLys: 1.815 ± 0.063
3.569TyrLeu: 3.569 ± 0.087
0.728TyrMet: 0.728 ± 0.043
1.206TyrAsn: 1.206 ± 0.053
1.501TyrPro: 1.501 ± 0.059
1.033TyrGln: 1.033 ± 0.054
1.697TyrArg: 1.697 ± 0.059
1.895TyrSer: 1.895 ± 0.077
1.674TyrThr: 1.674 ± 0.063
2.982TyrVal: 2.982 ± 0.072
0.516TyrTrp: 0.516 ± 0.034
1.365TyrTyr: 1.365 ± 0.054
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1481 proteins (439573 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski