Acinetobacter phage Ac42
Average proteome isoelectric point is 6.17
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 255 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|E5EYP9|E5EYP9_9CAUD Uncharacterized protein OS=Acinetobacter phage Ac42 OX=762660 GN=Ac42p127 PE=4 SV=1
MM1 pKa = 7.39 TLNEE5 pKa = 4.35 YY6 pKa = 10.16 IEE8 pKa = 4.04 NLKK11 pKa = 10.63 EE12 pKa = 4.18 FIKK15 pKa = 10.32 EE16 pKa = 3.82 NPGAGEE22 pKa = 4.29 LEE24 pKa = 4.65 VYY26 pKa = 10.37 ACSDD30 pKa = 4.58 DD31 pKa = 4.28 EE32 pKa = 5.03 GNNCNPVVFTPSKK45 pKa = 10.94 SYY47 pKa = 10.57 IDD49 pKa = 3.78 SDD51 pKa = 3.8 GEE53 pKa = 4.22 RR54 pKa = 11.84 YY55 pKa = 9.79 SEE57 pKa = 4.2 EE58 pKa = 4.78 DD59 pKa = 3.25 LDD61 pKa = 4.28 EE62 pKa = 5.07 YY63 pKa = 11.19 EE64 pKa = 4.94 ADD66 pKa = 3.63 EE67 pKa = 4.8 LSAIVIINN75 pKa = 3.48
Molecular weight: 8.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.839
IPC2_protein 3.694
IPC_protein 3.592
Toseland 3.427
ProMoST 3.757
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.478
Rodwell 3.439
Grimsley 3.35
Solomon 3.541
Lehninger 3.503
Nozaki 3.706
DTASelect 3.821
Thurlkill 3.478
EMBOSS 3.49
Sillero 3.719
Patrickios 0.401
IPC_peptide 3.541
IPC2_peptide 3.694
IPC2.peptide.svr19 3.675
Protein with the highest isoelectric point:
>tr|E5EYH8|E5EYH8_9CAUD Uncharacterized protein OS=Acinetobacter phage Ac42 OX=762660 GN=Ac42p056 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.29 CQDD5 pKa = 3.08 KK6 pKa = 10.01 TYY8 pKa = 10.54 EE9 pKa = 3.88 LLARR13 pKa = 11.84 LLEE16 pKa = 4.29 KK17 pKa = 11.04 NKK19 pKa = 10.16 LLKK22 pKa = 9.62 IDD24 pKa = 3.7 QMRR27 pKa = 11.84 VTSMKK32 pKa = 10.02 RR33 pKa = 11.84 RR34 pKa = 11.84 NIEE37 pKa = 3.73 SLKK40 pKa = 9.9 YY41 pKa = 10.47 SKK43 pKa = 11.05 NLLAIKK49 pKa = 10.0 NVNEE53 pKa = 4.6 DD54 pKa = 3.32 GRR56 pKa = 11.84 SSRR59 pKa = 11.84 MAKK62 pKa = 10.1 RR63 pKa = 11.84 LAKK66 pKa = 10.2 QVDD69 pKa = 4.24 KK70 pKa = 10.47 IQKK73 pKa = 10.28 RR74 pKa = 11.84 IDD76 pKa = 3.62 SLDD79 pKa = 3.33 KK80 pKa = 10.63 LQYY83 pKa = 10.79 NLFVEE88 pKa = 5.22 RR89 pKa = 11.84 KK90 pKa = 8.81 QIGLAIVKK98 pKa = 9.59 IRR100 pKa = 11.84 AFRR103 pKa = 11.84 NSARR107 pKa = 11.84 HH108 pKa = 4.52 FTTKK112 pKa = 10.51 GEE114 pKa = 3.76 HH115 pKa = 5.8 HH116 pKa = 6.29 MRR118 pKa = 11.84 YY119 pKa = 8.54 HH120 pKa = 6.46 QLLEE124 pKa = 4.27 DD125 pKa = 3.77 TLFTLSRR132 pKa = 11.84 TGYY135 pKa = 9.12 YY136 pKa = 10.66 SMRR139 pKa = 11.84 HH140 pKa = 4.88 YY141 pKa = 10.8 EE142 pKa = 4.47 WGDD145 pKa = 2.77 SWTSS149 pKa = 3.14
Molecular weight: 17.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.2
IPC2_protein 9.545
IPC_protein 9.663
Toseland 10.365
ProMoST 9.955
Dawson 10.496
Bjellqvist 10.131
Wikipedia 10.643
Rodwell 11.008
Grimsley 10.555
Solomon 10.54
Lehninger 10.511
Nozaki 10.335
DTASelect 10.131
Thurlkill 10.379
EMBOSS 10.745
Sillero 10.409
Patrickios 10.701
IPC_peptide 10.54
IPC2_peptide 8.697
IPC2.peptide.svr19 8.624
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
255
0
255
52675
18
1294
206.6
23.39
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.365 ± 0.185
1.109 ± 0.062
6.181 ± 0.108
6.823 ± 0.157
4.564 ± 0.106
5.895 ± 0.179
1.906 ± 0.076
7.026 ± 0.108
7.372 ± 0.195
8.133 ± 0.146
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.662 ± 0.084
5.657 ± 0.144
3.486 ± 0.081
3.569 ± 0.095
4.178 ± 0.098
6.77 ± 0.149
6.062 ± 0.227
6.944 ± 0.116
1.169 ± 0.061
4.123 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here