Amino acid dipepetide frequency for Acinetobacter phage Ac42

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.911AlaAla: 3.911 ± 0.322
0.57AlaCys: 0.57 ± 0.116
3.398AlaAsp: 3.398 ± 0.236
4.006AlaGlu: 4.006 ± 0.298
2.62AlaPhe: 2.62 ± 0.222
4.537AlaGly: 4.537 ± 0.37
1.082AlaHis: 1.082 ± 0.149
5.107AlaIle: 5.107 ± 0.306
4.936AlaLys: 4.936 ± 0.348
5.676AlaLeu: 5.676 ± 0.338
1.291AlaMet: 1.291 ± 0.161
3.683AlaAsn: 3.683 ± 0.287
2.05AlaPro: 2.05 ± 0.2
2.563AlaGln: 2.563 ± 0.241
2.639AlaArg: 2.639 ± 0.239
4.385AlaSer: 4.385 ± 0.339
3.949AlaThr: 3.949 ± 0.397
4.044AlaVal: 4.044 ± 0.268
0.949AlaTrp: 0.949 ± 0.11
2.259AlaTyr: 2.259 ± 0.202
0.0AlaXaa: 0.0 ± 0.0
Cys
0.645CysAla: 0.645 ± 0.102
0.152CysCys: 0.152 ± 0.062
0.608CysAsp: 0.608 ± 0.106
0.702CysGlu: 0.702 ± 0.107
0.551CysPhe: 0.551 ± 0.1
0.664CysGly: 0.664 ± 0.118
0.285CysHis: 0.285 ± 0.093
0.664CysIle: 0.664 ± 0.106
0.664CysLys: 0.664 ± 0.111
0.816CysLeu: 0.816 ± 0.135
0.361CysMet: 0.361 ± 0.084
0.721CysAsn: 0.721 ± 0.134
0.532CysPro: 0.532 ± 0.098
0.38CysGln: 0.38 ± 0.09
0.645CysArg: 0.645 ± 0.114
0.816CysSer: 0.816 ± 0.13
0.645CysThr: 0.645 ± 0.124
0.683CysVal: 0.683 ± 0.111
0.152CysTrp: 0.152 ± 0.058
0.399CysTyr: 0.399 ± 0.092
0.0CysXaa: 0.0 ± 0.0
Asp
3.835AspAla: 3.835 ± 0.266
0.721AspCys: 0.721 ± 0.109
3.417AspAsp: 3.417 ± 0.29
4.234AspGlu: 4.234 ± 0.262
3.493AspPhe: 3.493 ± 0.277
3.987AspGly: 3.987 ± 0.253
1.234AspHis: 1.234 ± 0.139
4.196AspIle: 4.196 ± 0.276
4.101AspLys: 4.101 ± 0.302
5.695AspLeu: 5.695 ± 0.328
1.652AspMet: 1.652 ± 0.175
2.601AspAsn: 2.601 ± 0.251
2.297AspPro: 2.297 ± 0.228
2.62AspGln: 2.62 ± 0.213
2.126AspArg: 2.126 ± 0.182
3.873AspSer: 3.873 ± 0.252
3.151AspThr: 3.151 ± 0.261
4.499AspVal: 4.499 ± 0.252
1.139AspTrp: 1.139 ± 0.161
2.943AspTyr: 2.943 ± 0.295
0.0AspXaa: 0.0 ± 0.0
Glu
4.746GluAla: 4.746 ± 0.416
1.044GluCys: 1.044 ± 0.136
3.474GluAsp: 3.474 ± 0.26
4.291GluGlu: 4.291 ± 0.333
3.038GluPhe: 3.038 ± 0.277
3.284GluGly: 3.284 ± 0.28
1.367GluHis: 1.367 ± 0.153
4.917GluIle: 4.917 ± 0.317
4.499GluLys: 4.499 ± 0.329
7.081GluLeu: 7.081 ± 0.433
2.031GluMet: 2.031 ± 0.215
3.778GluAsn: 3.778 ± 0.256
1.766GluPro: 1.766 ± 0.173
3.057GluGln: 3.057 ± 0.278
2.791GluArg: 2.791 ± 0.212
4.196GluSer: 4.196 ± 0.299
4.12GluThr: 4.12 ± 0.267
4.727GluVal: 4.727 ± 0.279
0.645GluTrp: 0.645 ± 0.115
3.36GluTyr: 3.36 ± 0.303
0.0GluXaa: 0.0 ± 0.0
Phe
2.734PheAla: 2.734 ± 0.228
0.304PheCys: 0.304 ± 0.076
3.911PheAsp: 3.911 ± 0.267
3.474PheGlu: 3.474 ± 0.262
1.443PhePhe: 1.443 ± 0.176
3.303PheGly: 3.303 ± 0.24
0.513PheHis: 0.513 ± 0.103
2.791PheIle: 2.791 ± 0.256
3.683PheLys: 3.683 ± 0.253
3.151PheLeu: 3.151 ± 0.249
1.158PheMet: 1.158 ± 0.165
2.848PheAsn: 2.848 ± 0.276
1.329PhePro: 1.329 ± 0.138
0.949PheGln: 0.949 ± 0.14
1.879PheArg: 1.879 ± 0.195
3.151PheSer: 3.151 ± 0.232
2.772PheThr: 2.772 ± 0.217
3.797PheVal: 3.797 ± 0.235
0.475PheTrp: 0.475 ± 0.103
1.974PheTyr: 1.974 ± 0.194
0.0PheXaa: 0.0 ± 0.0
Gly
3.607GlyAla: 3.607 ± 0.27
0.475GlyCys: 0.475 ± 0.09
3.55GlyAsp: 3.55 ± 0.295
3.645GlyGlu: 3.645 ± 0.249
2.772GlyPhe: 2.772 ± 0.229
3.038GlyGly: 3.038 ± 0.253
0.987GlyHis: 0.987 ± 0.143
4.044GlyIle: 4.044 ± 0.271
4.291GlyLys: 4.291 ± 0.346
4.936GlyLeu: 4.936 ± 0.35
1.936GlyMet: 1.936 ± 0.189
3.265GlyAsn: 3.265 ± 0.307
1.538GlyPro: 1.538 ± 0.129
2.468GlyGln: 2.468 ± 0.2
2.506GlyArg: 2.506 ± 0.168
4.423GlySer: 4.423 ± 0.415
4.177GlyThr: 4.177 ± 0.42
4.12GlyVal: 4.12 ± 0.263
0.778GlyTrp: 0.778 ± 0.164
2.392GlyTyr: 2.392 ± 0.248
0.0GlyXaa: 0.0 ± 0.0
His
0.968HisAla: 0.968 ± 0.156
0.171HisCys: 0.171 ± 0.056
1.025HisAsp: 1.025 ± 0.144
1.196HisGlu: 1.196 ± 0.142
0.873HisPhe: 0.873 ± 0.104
1.272HisGly: 1.272 ± 0.172
0.532HisHis: 0.532 ± 0.1
1.291HisIle: 1.291 ± 0.144
1.5HisLys: 1.5 ± 0.187
1.69HisLeu: 1.69 ± 0.197
0.342HisMet: 0.342 ± 0.079
1.025HisAsn: 1.025 ± 0.142
0.911HisPro: 0.911 ± 0.121
0.589HisGln: 0.589 ± 0.093
0.608HisArg: 0.608 ± 0.113
1.31HisSer: 1.31 ± 0.174
1.139HisThr: 1.139 ± 0.132
1.538HisVal: 1.538 ± 0.175
0.228HisTrp: 0.228 ± 0.073
0.854HisTyr: 0.854 ± 0.155
0.0HisXaa: 0.0 ± 0.0
Ile
4.993IleAla: 4.993 ± 0.38
0.987IleCys: 0.987 ± 0.146
5.733IleAsp: 5.733 ± 0.322
5.145IleGlu: 5.145 ± 0.338
2.525IlePhe: 2.525 ± 0.22
4.272IleGly: 4.272 ± 0.273
1.348IleHis: 1.348 ± 0.15
4.253IleIle: 4.253 ± 0.306
5.828IleLys: 5.828 ± 0.354
4.822IleLeu: 4.822 ± 0.321
1.519IleMet: 1.519 ± 0.162
4.329IleAsn: 4.329 ± 0.299
2.487IlePro: 2.487 ± 0.231
2.392IleGln: 2.392 ± 0.221
2.772IleArg: 2.772 ± 0.19
4.632IleSer: 4.632 ± 0.274
4.423IleThr: 4.423 ± 0.366
5.107IleVal: 5.107 ± 0.336
0.399IleTrp: 0.399 ± 0.091
2.297IleTyr: 2.297 ± 0.22
0.0IleXaa: 0.0 ± 0.0
Lys
5.088LysAla: 5.088 ± 0.372
0.778LysCys: 0.778 ± 0.137
4.12LysAsp: 4.12 ± 0.333
5.316LysGlu: 5.316 ± 0.338
3.664LysPhe: 3.664 ± 0.29
3.835LysGly: 3.835 ± 0.262
1.728LysHis: 1.728 ± 0.167
5.183LysIle: 5.183 ± 0.311
4.31LysLys: 4.31 ± 0.359
6.36LysLeu: 6.36 ± 0.382
2.525LysMet: 2.525 ± 0.235
3.74LysAsn: 3.74 ± 0.275
2.677LysPro: 2.677 ± 0.225
2.259LysGln: 2.259 ± 0.232
3.284LysArg: 3.284 ± 0.249
4.822LysSer: 4.822 ± 0.366
4.784LysThr: 4.784 ± 0.314
5.164LysVal: 5.164 ± 0.343
0.93LysTrp: 0.93 ± 0.124
3.151LysTyr: 3.151 ± 0.249
0.0LysXaa: 0.0 ± 0.0
Leu
5.619LeuAla: 5.619 ± 0.317
0.835LeuCys: 0.835 ± 0.144
5.525LeuAsp: 5.525 ± 0.368
6.303LeuGlu: 6.303 ± 0.415
3.702LeuPhe: 3.702 ± 0.273
3.987LeuGly: 3.987 ± 0.266
1.5LeuHis: 1.5 ± 0.172
5.582LeuIle: 5.582 ± 0.321
6.797LeuLys: 6.797 ± 0.426
5.43LeuLeu: 5.43 ± 0.35
2.449LeuMet: 2.449 ± 0.245
5.278LeuAsn: 5.278 ± 0.312
3.265LeuPro: 3.265 ± 0.269
3.038LeuGln: 3.038 ± 0.232
2.848LeuArg: 2.848 ± 0.197
5.714LeuSer: 5.714 ± 0.28
4.689LeuThr: 4.689 ± 0.338
4.841LeuVal: 4.841 ± 0.326
0.74LeuTrp: 0.74 ± 0.124
3.189LeuTyr: 3.189 ± 0.247
0.0LeuXaa: 0.0 ± 0.0
Met
1.823MetAla: 1.823 ± 0.197
0.323MetCys: 0.323 ± 0.097
1.5MetAsp: 1.5 ± 0.163
1.424MetGlu: 1.424 ± 0.167
0.987MetPhe: 0.987 ± 0.142
1.253MetGly: 1.253 ± 0.141
0.475MetHis: 0.475 ± 0.081
2.145MetIle: 2.145 ± 0.186
2.335MetLys: 2.335 ± 0.229
2.088MetLeu: 2.088 ± 0.192
0.683MetMet: 0.683 ± 0.118
2.031MetAsn: 2.031 ± 0.175
0.816MetPro: 0.816 ± 0.12
1.063MetGln: 1.063 ± 0.14
1.158MetArg: 1.158 ± 0.142
2.202MetSer: 2.202 ± 0.172
1.633MetThr: 1.633 ± 0.165
1.31MetVal: 1.31 ± 0.139
0.247MetTrp: 0.247 ± 0.077
1.12MetTyr: 1.12 ± 0.14
0.0MetXaa: 0.0 ± 0.0
Asn
3.607AsnAla: 3.607 ± 0.29
0.589AsnCys: 0.589 ± 0.122
3.322AsnAsp: 3.322 ± 0.281
3.569AsnGlu: 3.569 ± 0.305
2.601AsnPhe: 2.601 ± 0.21
4.101AsnGly: 4.101 ± 0.27
1.158AsnHis: 1.158 ± 0.154
4.158AsnIle: 4.158 ± 0.231
3.645AsnLys: 3.645 ± 0.277
4.784AsnLeu: 4.784 ± 0.281
1.576AsnMet: 1.576 ± 0.185
3.019AsnAsn: 3.019 ± 0.314
2.354AsnPro: 2.354 ± 0.23
2.107AsnGln: 2.107 ± 0.163
2.335AsnArg: 2.335 ± 0.177
3.797AsnSer: 3.797 ± 0.255
3.341AsnThr: 3.341 ± 0.275
3.968AsnVal: 3.968 ± 0.268
0.702AsnTrp: 0.702 ± 0.103
1.823AsnTyr: 1.823 ± 0.208
0.019AsnXaa: 0.019 ± 0.017
Pro
2.031ProAla: 2.031 ± 0.221
0.266ProCys: 0.266 ± 0.066
2.468ProAsp: 2.468 ± 0.222
2.943ProGlu: 2.943 ± 0.221
1.443ProPhe: 1.443 ± 0.198
2.126ProGly: 2.126 ± 0.202
0.551ProHis: 0.551 ± 0.1
2.753ProIle: 2.753 ± 0.234
2.81ProLys: 2.81 ± 0.216
2.411ProLeu: 2.411 ± 0.252
0.816ProMet: 0.816 ± 0.11
2.183ProAsn: 2.183 ± 0.178
0.93ProPro: 0.93 ± 0.13
0.74ProGln: 0.74 ± 0.142
1.329ProArg: 1.329 ± 0.179
2.449ProSer: 2.449 ± 0.203
2.05ProThr: 2.05 ± 0.212
2.43ProVal: 2.43 ± 0.243
0.361ProTrp: 0.361 ± 0.072
1.766ProTyr: 1.766 ± 0.168
0.0ProXaa: 0.0 ± 0.0
Gln
2.734GlnAla: 2.734 ± 0.234
0.342GlnCys: 0.342 ± 0.096
1.842GlnAsp: 1.842 ± 0.179
2.259GlnGlu: 2.259 ± 0.219
1.671GlnPhe: 1.671 ± 0.173
1.766GlnGly: 1.766 ± 0.199
0.57GlnHis: 0.57 ± 0.112
2.981GlnIle: 2.981 ± 0.241
1.728GlnLys: 1.728 ± 0.202
3.095GlnLeu: 3.095 ± 0.233
1.025GlnMet: 1.025 ± 0.127
2.069GlnAsn: 2.069 ± 0.209
1.196GlnPro: 1.196 ± 0.165
1.139GlnGln: 1.139 ± 0.154
1.614GlnArg: 1.614 ± 0.187
2.582GlnSer: 2.582 ± 0.193
2.202GlnThr: 2.202 ± 0.196
2.658GlnVal: 2.658 ± 0.245
0.475GlnTrp: 0.475 ± 0.087
1.747GlnTyr: 1.747 ± 0.185
0.0GlnXaa: 0.0 ± 0.0
Arg
2.582ArgAla: 2.582 ± 0.238
0.456ArgCys: 0.456 ± 0.096
2.373ArgAsp: 2.373 ± 0.204
2.943ArgGlu: 2.943 ± 0.253
2.24ArgPhe: 2.24 ± 0.201
2.525ArgGly: 2.525 ± 0.229
0.987ArgHis: 0.987 ± 0.13
3.0ArgIle: 3.0 ± 0.236
2.734ArgLys: 2.734 ± 0.227
3.911ArgLeu: 3.911 ± 0.247
1.158ArgMet: 1.158 ± 0.144
2.05ArgAsn: 2.05 ± 0.192
1.272ArgPro: 1.272 ± 0.152
1.443ArgGln: 1.443 ± 0.16
2.031ArgArg: 2.031 ± 0.25
2.544ArgSer: 2.544 ± 0.211
2.525ArgThr: 2.525 ± 0.202
2.867ArgVal: 2.867 ± 0.229
0.475ArgTrp: 0.475 ± 0.109
1.652ArgTyr: 1.652 ± 0.174
0.019ArgXaa: 0.019 ± 0.017
Ser
3.873SerAla: 3.873 ± 0.266
0.74SerCys: 0.74 ± 0.127
4.082SerAsp: 4.082 ± 0.267
4.196SerGlu: 4.196 ± 0.299
3.038SerPhe: 3.038 ± 0.253
4.48SerGly: 4.48 ± 0.316
1.158SerHis: 1.158 ± 0.129
5.012SerIle: 5.012 ± 0.33
5.43SerLys: 5.43 ± 0.392
5.221SerLeu: 5.221 ± 0.318
1.709SerMet: 1.709 ± 0.171
3.987SerAsn: 3.987 ± 0.297
2.126SerPro: 2.126 ± 0.238
2.411SerGln: 2.411 ± 0.209
3.208SerArg: 3.208 ± 0.195
4.556SerSer: 4.556 ± 0.316
4.651SerThr: 4.651 ± 0.398
4.215SerVal: 4.215 ± 0.271
0.759SerTrp: 0.759 ± 0.118
2.848SerTyr: 2.848 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
3.531ThrAla: 3.531 ± 0.324
0.664ThrCys: 0.664 ± 0.104
3.398ThrAsp: 3.398 ± 0.265
4.101ThrGlu: 4.101 ± 0.327
2.962ThrPhe: 2.962 ± 0.242
4.291ThrGly: 4.291 ± 0.336
1.101ThrHis: 1.101 ± 0.14
4.196ThrIle: 4.196 ± 0.302
4.082ThrLys: 4.082 ± 0.28
4.613ThrLeu: 4.613 ± 0.366
1.5ThrMet: 1.5 ± 0.188
3.038ThrAsn: 3.038 ± 0.263
2.506ThrPro: 2.506 ± 0.229
2.031ThrGln: 2.031 ± 0.24
2.715ThrArg: 2.715 ± 0.246
4.272ThrSer: 4.272 ± 0.337
3.607ThrThr: 3.607 ± 0.361
4.727ThrVal: 4.727 ± 0.349
0.778ThrTrp: 0.778 ± 0.121
2.506ThrTyr: 2.506 ± 0.216
0.0ThrXaa: 0.0 ± 0.0
Val
3.949ValAla: 3.949 ± 0.293
0.911ValCys: 0.911 ± 0.146
4.347ValAsp: 4.347 ± 0.299
5.43ValGlu: 5.43 ± 0.342
3.455ValPhe: 3.455 ± 0.226
3.17ValGly: 3.17 ± 0.254
1.462ValHis: 1.462 ± 0.184
4.822ValIle: 4.822 ± 0.303
5.904ValLys: 5.904 ± 0.327
5.619ValLeu: 5.619 ± 0.351
1.519ValMet: 1.519 ± 0.164
3.835ValAsn: 3.835 ± 0.33
2.658ValPro: 2.658 ± 0.272
2.62ValGln: 2.62 ± 0.335
3.038ValArg: 3.038 ± 0.244
4.366ValSer: 4.366 ± 0.261
3.816ValThr: 3.816 ± 0.394
4.803ValVal: 4.803 ± 0.297
0.74ValTrp: 0.74 ± 0.115
2.981ValTyr: 2.981 ± 0.26
0.0ValXaa: 0.0 ± 0.0
Trp
0.797TrpAla: 0.797 ± 0.139
0.171TrpCys: 0.171 ± 0.052
0.816TrpAsp: 0.816 ± 0.144
0.816TrpGlu: 0.816 ± 0.111
0.513TrpPhe: 0.513 ± 0.096
0.551TrpGly: 0.551 ± 0.112
0.209TrpHis: 0.209 ± 0.063
0.513TrpIle: 0.513 ± 0.1
1.196TrpLys: 1.196 ± 0.154
0.854TrpLeu: 0.854 ± 0.118
0.418TrpMet: 0.418 ± 0.078
0.645TrpAsn: 0.645 ± 0.103
0.342TrpPro: 0.342 ± 0.067
0.418TrpGln: 0.418 ± 0.096
0.38TrpArg: 0.38 ± 0.09
0.816TrpSer: 0.816 ± 0.137
0.589TrpThr: 0.589 ± 0.094
1.12TrpVal: 1.12 ± 0.139
0.095TrpTrp: 0.095 ± 0.044
0.437TrpTyr: 0.437 ± 0.097
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.582TyrAla: 2.582 ± 0.203
0.589TyrCys: 0.589 ± 0.102
2.905TyrAsp: 2.905 ± 0.304
2.24TyrGlu: 2.24 ± 0.197
2.05TyrPhe: 2.05 ± 0.197
2.506TyrGly: 2.506 ± 0.233
0.816TyrHis: 0.816 ± 0.134
2.658TyrIle: 2.658 ± 0.232
3.246TyrLys: 3.246 ± 0.256
3.076TyrLeu: 3.076 ± 0.233
0.911TyrMet: 0.911 ± 0.13
2.449TyrAsn: 2.449 ± 0.227
1.804TyrPro: 1.804 ± 0.169
1.386TyrGln: 1.386 ± 0.162
1.879TyrArg: 1.879 ± 0.219
2.791TyrSer: 2.791 ± 0.227
2.354TyrThr: 2.354 ± 0.219
2.829TyrVal: 2.829 ± 0.251
0.626TyrTrp: 0.626 ± 0.126
1.538TyrTyr: 1.538 ± 0.208
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.019XaaMet: 0.019 ± 0.017
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.019XaaGln: 0.019 ± 0.017
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.019XaaXaa: 0.019 ± 0.017
Statistics based on 255 proteins (52675 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski