Streptomyces gancidicus BKS 13-15

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces; Streptomyces gancidicus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6555 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M3DXF3|M3DXF3_9ACTN Crotonyl CoA reductase OS=Streptomyces gancidicus BKS 13-15 OX=1284664 GN=H114_25179 PE=4 SV=1
MM1 pKa = 7.27TSRR4 pKa = 11.84GKK6 pKa = 7.63TLKK9 pKa = 9.36LTAVSTLVVLALTGFSTGRR28 pKa = 11.84GHH30 pKa = 7.26GGGSGSHH37 pKa = 7.14DD38 pKa = 3.28GGGGGGCSSSSQNHH52 pKa = 6.65DD53 pKa = 3.46SSSGGSHH60 pKa = 7.03RR61 pKa = 11.84DD62 pKa = 3.45YY63 pKa = 11.57YY64 pKa = 11.46DD65 pKa = 5.18DD66 pKa = 6.14DD67 pKa = 7.19DD68 pKa = 6.59YY69 pKa = 12.09DD70 pKa = 4.98DD71 pKa = 6.11DD72 pKa = 4.81YY73 pKa = 12.1GSGSSGGSEE82 pKa = 3.64ATASPEE88 pKa = 3.96DD89 pKa = 3.62ATVTLVDD96 pKa = 4.22CASEE100 pKa = 4.09EE101 pKa = 4.21TPYY104 pKa = 10.67ATVEE108 pKa = 4.14VTNDD112 pKa = 3.02STTSGLFSVTVVFRR126 pKa = 11.84DD127 pKa = 3.36AAGEE131 pKa = 4.0EE132 pKa = 4.43LVSRR136 pKa = 11.84TEE138 pKa = 4.6DD139 pKa = 3.0IFVTEE144 pKa = 4.44GQTTPARR151 pKa = 11.84IEE153 pKa = 3.97VDD155 pKa = 3.89DD156 pKa = 4.33EE157 pKa = 4.88ASVPLIDD164 pKa = 5.2EE165 pKa = 5.05CDD167 pKa = 3.85LDD169 pKa = 5.52DD170 pKa = 4.45YY171 pKa = 12.01APAAA175 pKa = 4.17

Molecular weight: 17.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M3CT10|M3CT10_9ACTN Uncharacterized protein OS=Streptomyces gancidicus BKS 13-15 OX=1284664 GN=H114_19485 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILASRR35 pKa = 11.84RR36 pKa = 11.84SKK38 pKa = 10.75GRR40 pKa = 11.84ARR42 pKa = 11.84LSAA45 pKa = 3.91

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6555

0

6555

2106892

22

3847

321.4

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.674 ± 0.053

0.764 ± 0.009

6.248 ± 0.028

5.883 ± 0.029

2.66 ± 0.016

9.485 ± 0.029

2.314 ± 0.015

2.829 ± 0.02

2.029 ± 0.025

10.345 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.693 ± 0.012

1.656 ± 0.017

6.191 ± 0.029

2.622 ± 0.019

8.402 ± 0.034

4.772 ± 0.021

6.161 ± 0.025

8.714 ± 0.028

1.511 ± 0.012

2.046 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski