Zetapapillomavirus 1
Average proteome isoelectric point is 5.95
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q8BE76|Q8BE76_9PAPI Protein E6 OS=Zetapapillomavirus 1 OX=333919 GN=E6 PE=3 SV=1
MM1 pKa = 7.66 IGNGSPSLRR10 pKa = 11.84 EE11 pKa = 3.71 IVLSEE16 pKa = 4.4 LPQSLADD23 pKa = 3.64 PAEE26 pKa = 4.43 AEE28 pKa = 4.24 SEE30 pKa = 4.2 EE31 pKa = 4.48 EE32 pKa = 4.04 EE33 pKa = 5.03 VEE35 pKa = 4.29 VEE37 pKa = 3.95 LDD39 pKa = 3.34 AVRR42 pKa = 11.84 PQAPYY47 pKa = 10.4 AVCTVCCRR55 pKa = 11.84 CGEE58 pKa = 4.17 KK59 pKa = 10.62 VGLCVLATDD68 pKa = 4.21 EE69 pKa = 5.31 GIHH72 pKa = 6.0 GLEE75 pKa = 3.84 EE76 pKa = 4.25 LLFEE80 pKa = 4.98 ALQLFCAQCAPPIGRR95 pKa = 11.84 HH96 pKa = 4.7 GRR98 pKa = 3.45
Molecular weight: 10.51 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.078
IPC2_protein 4.368
IPC_protein 4.228
Toseland 4.088
ProMoST 4.304
Dawson 4.139
Bjellqvist 4.291
Wikipedia 3.973
Rodwell 4.062
Grimsley 4.012
Solomon 4.139
Lehninger 4.088
Nozaki 4.266
DTASelect 4.304
Thurlkill 4.088
EMBOSS 3.999
Sillero 4.329
Patrickios 0.947
IPC_peptide 4.151
IPC2_peptide 4.317
IPC2.peptide.svr19 4.238
Protein with the highest isoelectric point:
>tr|Q8BE74|Q8BE74_9PAPI Replication protein E1 OS=Zetapapillomavirus 1 OX=333919 GN=E1 PE=3 SV=1
MM1 pKa = 7.42 MEE3 pKa = 4.48 TLRR6 pKa = 11.84 QRR8 pKa = 11.84 LDD10 pKa = 3.21 AVQEE14 pKa = 4.0 KK15 pKa = 10.52 LMNLLEE21 pKa = 4.72 EE22 pKa = 4.9 GSSDD26 pKa = 4.07 LSSQICYY33 pKa = 7.63 WQAVRR38 pKa = 11.84 KK39 pKa = 9.68 EE40 pKa = 4.02 NVLLYY45 pKa = 9.07 YY46 pKa = 10.6 ARR48 pKa = 11.84 EE49 pKa = 4.09 KK50 pKa = 10.94 GLSRR54 pKa = 11.84 LGLQMVPHH62 pKa = 6.81 KK63 pKa = 10.68 AVSQSQAKK71 pKa = 8.67 QAIHH75 pKa = 6.38 MEE77 pKa = 4.89 LILLSLQGSSYY88 pKa = 9.39 EE89 pKa = 4.03 QEE91 pKa = 3.72 PWTLSDD97 pKa = 4.25 CSWEE101 pKa = 4.01 RR102 pKa = 11.84 WLQAPINCLKK112 pKa = 9.89 KK113 pKa = 10.59 DD114 pKa = 3.66 PVIVEE119 pKa = 3.73 VVYY122 pKa = 11.0 DD123 pKa = 4.07 GNSEE127 pKa = 3.9 NANWYY132 pKa = 6.44 TLWGLIYY139 pKa = 10.13 YY140 pKa = 7.53 QTFEE144 pKa = 4.39 GDD146 pKa = 3.35 WMCTRR151 pKa = 11.84 GQCDD155 pKa = 3.0 HH156 pKa = 6.96 SGLYY160 pKa = 10.06 YY161 pKa = 10.53 EE162 pKa = 4.89 EE163 pKa = 5.05 EE164 pKa = 3.96 GHH166 pKa = 5.99 KK167 pKa = 9.97 RR168 pKa = 11.84 YY169 pKa = 9.47 YY170 pKa = 10.48 VHH172 pKa = 7.48 FIDD175 pKa = 5.56 DD176 pKa = 3.59 AARR179 pKa = 11.84 YY180 pKa = 9.13 SKK182 pKa = 9.66 TRR184 pKa = 11.84 TWEE187 pKa = 3.86 VRR189 pKa = 11.84 CRR191 pKa = 11.84 NQIYY195 pKa = 10.2 LPSIPVTSTPPQSPSHH211 pKa = 6.77 IDD213 pKa = 3.6 LPDD216 pKa = 3.71 GAAGGGPNQSPRR228 pKa = 11.84 PGALAVSPQEE238 pKa = 4.14 PPKK241 pKa = 10.55 KK242 pKa = 9.74 RR243 pKa = 11.84 YY244 pKa = 9.44 RR245 pKa = 11.84 SPADD249 pKa = 3.41 TVSSSRR255 pKa = 11.84 LSGGLRR261 pKa = 11.84 CPADD265 pKa = 2.85 WCRR268 pKa = 11.84 RR269 pKa = 11.84 KK270 pKa = 9.89 LQRR273 pKa = 11.84 TSAPTWVPPSVSEE286 pKa = 4.32 VPEE289 pKa = 4.16 APEE292 pKa = 4.36 GSVSEE297 pKa = 4.37 TGGASPGVDD306 pKa = 2.73 STTGRR311 pKa = 11.84 GNDD314 pKa = 3.76 PAPVPLEE321 pKa = 3.76 AAFAPIVIFQGGTNQCKK338 pKa = 9.99 CYY340 pKa = 9.91 RR341 pKa = 11.84 WRR343 pKa = 11.84 LKK345 pKa = 10.08 KK346 pKa = 9.96 RR347 pKa = 11.84 HH348 pKa = 6.22 RR349 pKa = 11.84 SLFVAITTTYY359 pKa = 10.23 FWTGDD364 pKa = 3.1 KK365 pKa = 10.92 GGQRR369 pKa = 11.84 VGNARR374 pKa = 11.84 LMVTFSSDD382 pKa = 2.98 LQRR385 pKa = 11.84 RR386 pKa = 11.84 LLLATVPPPRR396 pKa = 11.84 GVTATSFTLTPSS408 pKa = 3.08
Molecular weight: 45.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.529
IPC2_protein 7.79
IPC_protein 7.629
Toseland 7.19
ProMoST 8.17
Dawson 8.317
Bjellqvist 8.712
Wikipedia 8.244
Rodwell 8.331
Grimsley 7.205
Solomon 8.39
Lehninger 8.404
Nozaki 8.873
DTASelect 8.419
Thurlkill 8.463
EMBOSS 8.521
Sillero 8.799
Patrickios 4.177
IPC_peptide 8.39
IPC2_peptide 7.805
IPC2.peptide.svr19 7.904
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6
0
6
2248
98
620
374.7
41.76
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.45 ± 0.488
2.224 ± 0.704
5.694 ± 0.568
6.361 ± 0.636
3.826 ± 0.572
7.829 ± 1.116
2.002 ± 0.113
3.692 ± 0.273
3.915 ± 0.734
8.763 ± 0.464
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.957 ± 0.226
3.781 ± 0.684
7.073 ± 0.708
4.181 ± 0.593
6.717 ± 0.362
7.473 ± 0.724
5.738 ± 0.53
7.251 ± 0.475
1.957 ± 0.358
3.114 ± 0.253
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here