Mycobacterium phage Rabbs

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Liefievirus; unclassified Liefievirus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A481VT29|A0A481VT29_9CAUD Uncharacterized protein OS=Mycobacterium phage Rabbs OX=2530143 GN=56 PE=4 SV=1
MM1 pKa = 7.32MGTLGALCGLAGDD14 pKa = 4.44ALIAVGFALRR24 pKa = 11.84EE25 pKa = 3.98HH26 pKa = 7.08DD27 pKa = 4.93RR28 pKa = 11.84GFSDD32 pKa = 5.1YY33 pKa = 11.23EE34 pKa = 4.98AGDD37 pKa = 3.76YY38 pKa = 9.18LAPAEE43 pKa = 4.71PPAPTDD49 pKa = 3.87DD50 pKa = 4.47LSIPLGDD57 pKa = 3.46VRR59 pKa = 11.84AALDD63 pKa = 4.04DD64 pKa = 4.62LSDD67 pKa = 3.91SKK69 pKa = 11.36LLNIAATIIAGWKK82 pKa = 9.54PILLKK87 pKa = 8.89TTGDD91 pKa = 3.55LTDD94 pKa = 3.23VDD96 pKa = 4.41VLVDD100 pKa = 3.79ALRR103 pKa = 11.84DD104 pKa = 3.36RR105 pKa = 11.84ATQYY109 pKa = 11.77AVVEE113 pKa = 4.37ADD115 pKa = 4.45DD116 pKa = 4.03VPPAAGAPNSSPEE129 pKa = 3.93RR130 pKa = 11.84GEE132 pKa = 3.83

Molecular weight: 13.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A481VT35|A0A481VT35_9CAUD Uncharacterized protein OS=Mycobacterium phage Rabbs OX=2530143 GN=46 PE=4 SV=1
MM1 pKa = 7.07SWEE4 pKa = 4.87PILAALGGYY13 pKa = 10.12LFFAGRR19 pKa = 11.84DD20 pKa = 3.35VFRR23 pKa = 11.84AVMQSEE29 pKa = 4.28WMFGRR34 pKa = 11.84ILAADD39 pKa = 3.76LRR41 pKa = 11.84VIDD44 pKa = 3.55RR45 pKa = 11.84AQRR48 pKa = 11.84RR49 pKa = 11.84KK50 pKa = 10.45AKK52 pKa = 10.39

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

13836

42

1341

212.9

23.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.103 ± 0.612

0.882 ± 0.138

6.931 ± 0.307

5.789 ± 0.401

3.007 ± 0.182

9.027 ± 0.524

2.175 ± 0.199

4.568 ± 0.205

3.469 ± 0.255

7.596 ± 0.235

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.125 ± 0.122

3.108 ± 0.193

6.172 ± 0.368

3.361 ± 0.192

7.04 ± 0.506

5.066 ± 0.33

5.934 ± 0.253

6.678 ± 0.217

1.988 ± 0.182

1.98 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski